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The map label for this gene is ansP [H]

Identifier: 158423618

GI number: 158423618

Start: 2297975

End: 2299423

Strand: Reverse

Name: ansP [H]

Synonym: AZC_1994

Alternate gene names: 158423618

Gene position: 2299423-2297975 (Counterclockwise)

Preceding gene: 158423619

Following gene: 158423616

Centisome position: 42.82

GC content: 65.77

Gene sequence:

>1449_bases
GTGGCGGGCATGACGATGGCGACTTCAGAAAACCAAACTGAGGCGCCGTCCCCACAGGACAAGGCCGCCTGGCTCGCCTC
CCACGAGGAGGGCTACCATCAGGGCCTCGGCAACCGCCACATCCAGATGATCGCCATCGGCGGCGCCATCGGGACCGGCC
TTTTCCTCGGGGCCGGCTCCCGCCTCCAGGCGGCCGGTCCGTCGCTCGCCCTCGTCTATCTGGTGTGCGGCGTCTTCGCC
TTCCTGATCCTGCGGGCGCTCGGGGAACTGGTGCTGCACCGGCCGAGCAGCGGCAGCTTCGTCTCCTATGCGCGCGAATT
CCTCGGCGAGAAGGCCTCCTTCGTCGCGGGCTGGCTCTATTTCCTCAACTGGGCGATGACGGGCATCGTCGATATCACGG
CGGTCGCGCTCTACTTCAAGTTCTGGATGGTCTTCCAGGATGTGCCGCAATGGGTGTTCGCCCTCGGCGCCCTGTGCGTC
GTCACCTCGCTGAACCTCATCAATGTCAAATGGTTCGGCGAGATGGAGTTCTGGTTCGCGCTGGTGAAGGTGGCGGCACT
TGTGATCTTTCTCGCCGTCGGCGTCATCGTGCTGGTCATGGGCATTCCGGTCGCCGGCCATACCACGGGCCTCCACCTCA
TCACCGAAAATGGCGGCTTCTTCCCGCACGGGGTGCTGCCGGCGCTGGTGCTGGTGCAGGGTGTCGTCTTCGCATATGCG
GGCATCGAACTCGTCGGCATCACCTCGGGCGAGTGCAAGGATGCGCGCAAGGTGCTGCCGGCGGCCATCAACGGCGTCAT
CTGGCGTATCGCCCTCTTCTATGTGGGCTCGGTGGTGCTGCTGGTCTGCCTGCTCCCGTGGAACGTGTACAGTGCCGGGG
AGAGCCCCTTCGTCACCTTCTTCACCGCCATCGGCTCGCCGGGCAACGGCTCGATCATGAATGTGGTGGTGCTGACCGCT
GCCCTGTCGAGCCTCAATTCCGGTCTCTATTCCACCGGGCGCGTGCTGCGCTCGCTCTCCATGGGCGGCTCGGCACCGCC
GCTGCTGGCGCGCCTCAACCGCCATTCGGTGCCCTTCGCGGGCATCATGATTACCGTGAGCTTCTATGCGGTGGGCGTCG
TGCTGAATTATCTCGTGCCGACGCGCGTGTTCGAGATCGTGCTGAACGTGGCCTCGCTCGGCATCCTCAGCACCTGGGCC
TTCATCCTCGTGTGCCAGCTGAAGCTCCGGCAGGCGGTGAAGCGCGGCGAGGCGCAGCCCGTCTCCTTCCGCATGCCGGG
CGCGCCCTTCACCACATGGCTGTCGCTGGCCTTCCTCGCCGGCGTGCTGGTGCTGATGGCCTTCGATTATCCGGACGGCA
CGCTGACCGTCGCCATGATCCCCCTGCTGGCGGTGCTGCTCGTCCTCGGCTGGCACCTCCTGAAGCGCGGAAAGCCGGCG
CGCGCCTGA

Upstream 100 bases:

>100_bases
GACAGGGTGATGAAGCCGATAAAAAGCACACCCCACACCCCGTTACCCGCATACTGATACCGACTACGGCTAGGTTGCCG
CGCAGGGCATCAGCGTTTGG

Downstream 100 bases:

>100_bases
TCAGGCCACCTGCGGCGCGGCGGCACGCAAGGCCGCGCCGCCTATCACATCGCCCATGAATTCCGCGATGACGCTTTCGA
GCGTCGGCCCCACATCCACA

Product: amino acid permease

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MAGMTMATSENQTEAPSPQDKAAWLASHEEGYHQGLGNRHIQMIAIGGAIGTGLFLGAGSRLQAAGPSLALVYLVCGVFA
FLILRALGELVLHRPSSGSFVSYAREFLGEKASFVAGWLYFLNWAMTGIVDITAVALYFKFWMVFQDVPQWVFALGALCV
VTSLNLINVKWFGEMEFWFALVKVAALVIFLAVGVIVLVMGIPVAGHTTGLHLITENGGFFPHGVLPALVLVQGVVFAYA
GIELVGITSGECKDARKVLPAAINGVIWRIALFYVGSVVLLVCLLPWNVYSAGESPFVTFFTAIGSPGNGSIMNVVVLTA
ALSSLNSGLYSTGRVLRSLSMGGSAPPLLARLNRHSVPFAGIMITVSFYAVGVVLNYLVPTRVFEIVLNVASLGILSTWA
FILVCQLKLRQAVKRGEAQPVSFRMPGAPFTTWLSLAFLAGVLVLMAFDYPDGTLTVAMIPLLAVLLVLGWHLLKRGKPA
RA

Sequences:

>Translated_482_residues
MAGMTMATSENQTEAPSPQDKAAWLASHEEGYHQGLGNRHIQMIAIGGAIGTGLFLGAGSRLQAAGPSLALVYLVCGVFA
FLILRALGELVLHRPSSGSFVSYAREFLGEKASFVAGWLYFLNWAMTGIVDITAVALYFKFWMVFQDVPQWVFALGALCV
VTSLNLINVKWFGEMEFWFALVKVAALVIFLAVGVIVLVMGIPVAGHTTGLHLITENGGFFPHGVLPALVLVQGVVFAYA
GIELVGITSGECKDARKVLPAAINGVIWRIALFYVGSVVLLVCLLPWNVYSAGESPFVTFFTAIGSPGNGSIMNVVVLTA
ALSSLNSGLYSTGRVLRSLSMGGSAPPLLARLNRHSVPFAGIMITVSFYAVGVVLNYLVPTRVFEIVLNVASLGILSTWA
FILVCQLKLRQAVKRGEAQPVSFRMPGAPFTTWLSLAFLAGVLVLMAFDYPDGTLTVAMIPLLAVLLVLGWHLLKRGKPA
RA
>Mature_481_residues
AGMTMATSENQTEAPSPQDKAAWLASHEEGYHQGLGNRHIQMIAIGGAIGTGLFLGAGSRLQAAGPSLALVYLVCGVFAF
LILRALGELVLHRPSSGSFVSYAREFLGEKASFVAGWLYFLNWAMTGIVDITAVALYFKFWMVFQDVPQWVFALGALCVV
TSLNLINVKWFGEMEFWFALVKVAALVIFLAVGVIVLVMGIPVAGHTTGLHLITENGGFFPHGVLPALVLVQGVVFAYAG
IELVGITSGECKDARKVLPAAINGVIWRIALFYVGSVVLLVCLLPWNVYSAGESPFVTFFTAIGSPGNGSIMNVVVLTAA
LSSLNSGLYSTGRVLRSLSMGGSAPPLLARLNRHSVPFAGIMITVSFYAVGVVLNYLVPTRVFEIVLNVASLGILSTWAF
ILVCQLKLRQAVKRGEAQPVSFRMPGAPFTTWLSLAFLAGVLVLMAFDYPDGTLTVAMIPLLAVLLVLGWHLLKRGKPAR
A

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081915, Length=469, Percent_Identity=68.6567164179104, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=465, Percent_Identity=37.8494623655914, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1786302, Length=458, Percent_Identity=39.5196506550218, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1790653, Length=460, Percent_Identity=37.6086956521739, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI48994972, Length=444, Percent_Identity=39.6396396396396, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1786602, Length=448, Percent_Identity=37.7232142857143, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1789017, Length=452, Percent_Identity=33.1858407079646, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87081708, Length=454, Percent_Identity=32.3788546255507, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1788480, Length=415, Percent_Identity=33.7349397590361, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI87081869, Length=196, Percent_Identity=26.530612244898, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320772, Length=472, Percent_Identity=30.5084745762712, Blast_Score=199, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6324061, Length=461, Percent_Identity=29.5010845986985, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322892, Length=429, Percent_Identity=33.5664335664336, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6324924, Length=439, Percent_Identity=30.9794988610478, Blast_Score=172, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6324990, Length=463, Percent_Identity=28.9416846652268, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322967, Length=412, Percent_Identity=29.6116504854369, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324059, Length=461, Percent_Identity=30.8026030368764, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324981, Length=416, Percent_Identity=31.25, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321629, Length=407, Percent_Identity=27.7641277641278, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321053, Length=467, Percent_Identity=27.4089935760171, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6324553, Length=396, Percent_Identity=31.3131313131313, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319824, Length=436, Percent_Identity=26.605504587156, Blast_Score=146, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6319543, Length=436, Percent_Identity=26.8348623853211, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320717, Length=415, Percent_Identity=26.7469879518072, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319608, Length=469, Percent_Identity=24.9466950959488, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320251, Length=425, Percent_Identity=25.1764705882353, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319542, Length=429, Percent_Identity=24.2424242424242, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320364, Length=517, Percent_Identity=21.6634429400387, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51581; Mature: 51450

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGMTMATSENQTEAPSPQDKAAWLASHEEGYHQGLGNRHIQMIAIGGAIGTGLFLGAGS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCC
RLQAAGPSLALVYLVCGVFAFLILRALGELVLHRPSSGSFVSYAREFLGEKASFVAGWLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
FLNWAMTGIVDITAVALYFKFWMVFQDVPQWVFALGALCVVTSLNLINVKWFGEMEFWFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHH
LVKVAALVIFLAVGVIVLVMGIPVAGHTTGLHLITENGGFFPHGVLPALVLVQGVVFAYA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
GIELVGITSGECKDARKVLPAAINGVIWRIALFYVGSVVLLVCLLPWNVYSAGESPFVTF
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHH
FTAIGSPGNGSIMNVVVLTAALSSLNSGLYSTGRVLRSLSMGGSAPPLLARLNRHSVPFA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHH
GIMITVSFYAVGVVLNYLVPTRVFEIVLNVASLGILSTWAFILVCQLKLRQAVKRGEAQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSFRMPGAPFTTWLSLAFLAGVLVLMAFDYPDGTLTVAMIPLLAVLLVLGWHLLKRGKPA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RA
CC
>Mature Secondary Structure 
AGMTMATSENQTEAPSPQDKAAWLASHEEGYHQGLGNRHIQMIAIGGAIGTGLFLGAGS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCC
RLQAAGPSLALVYLVCGVFAFLILRALGELVLHRPSSGSFVSYAREFLGEKASFVAGWLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
FLNWAMTGIVDITAVALYFKFWMVFQDVPQWVFALGALCVVTSLNLINVKWFGEMEFWFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHH
LVKVAALVIFLAVGVIVLVMGIPVAGHTTGLHLITENGGFFPHGVLPALVLVQGVVFAYA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
GIELVGITSGECKDARKVLPAAINGVIWRIALFYVGSVVLLVCLLPWNVYSAGESPFVTF
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHH
FTAIGSPGNGSIMNVVVLTAALSSLNSGLYSTGRVLRSLSMGGSAPPLLARLNRHSVPFA
HHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHH
GIMITVSFYAVGVVLNYLVPTRVFEIVLNVASLGILSTWAFILVCQLKLRQAVKRGEAQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSFRMPGAPFTTWLSLAFLAGVLVLMAFDYPDGTLTVAMIPLLAVLLVLGWHLLKRGKPA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
RA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]