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The map label for this gene is gsiB [H]

Identifier: 158423610

GI number: 158423610

Start: 2289233

End: 2290819

Strand: Reverse

Name: gsiB [H]

Synonym: AZC_1986

Alternate gene names: 158423610

Gene position: 2290819-2289233 (Counterclockwise)

Preceding gene: 158423612

Following gene: 158423609

Centisome position: 42.66

GC content: 63.64

Gene sequence:

>1587_bases
ATGCGTCGGCACTTTTCGACCCTTCTCGCCACCGTGATGGTGGGCCTGTGCCTCGGCGCCGCCGCCGATGCCAAGACCTT
CCGCTGGGCGCGCGGCCAGGACGCACCCACCCTCGATCCTCACACCGAGAATTCGAACCAGGTCTTCGGCCTGCTGCACC
AGTTCTATGAACCGCTCGTGCAGCGCGACGAGACGGGCCAACTGGTGCCCTGCCTCGCGCTGAGCTGGGAACTGAAGCCG
AGCGACCCGACTGTCTGGATCTTCAAGCTGCGGCCGAACGTCACCTTTCACAATGGCGACAAGTTCACCGCCGACGACGC
CGTCTTCTCCCTGAAGCGCGCCATGGACCCGCTGGCGGGCCTGCGCACCACGCTCGCCGGTGTCACCGACGTGACCAAGG
TGGACGACCTCACCATCGAGGTGAAGACGGCGGCCCCCATGCCGCTGCTGGTGAACCAGATCACCAATGTCTTCATGATG
GACAAGAAGTGGGCCGAGGCGAACAACGTCGCCCGCCCGCAGGACTTCAAGAACAAGGAAGAGACCTTCGCCGTCCGCAA
CGAGAACGGCACCGGTCCCTATATCGTCACCAGCCGCGAGCCGGACTCACGCACGGTGATGAAGCTGAACGACGCCTATT
GGGGCAAGGGCCAGTTCCCGCTCCAGGCCACGGACGTCGTCTATAGCGTCATCGCCTCCGGCCCCACCCGCGTCGCCGCC
TTGCTCTCGGGCGAGGTGGACTTCGTGCAGGACATGCCGGTGCAGGACATTGATCGCGTGGCCCAGACGCCTGGCCTCAA
GGTGAATACGGGCGCCGAAGTCCGCTCCATCTTCTTCGGCCTCAACGGCGGTTCGCCGGAACTGCCCTCCTCCGACGTGA
AGGGCAAGAACCCGCTGGCCGACAAGCGCGTGCGCCAGGCCATCGAGATCGCCATCAACCGCGAGGCGATCCAGAAGGTG
GTGATGGGCGGCCAGTCCGCCCCCTCCGGCACGCTCATCCCGCCCGGCGTGAACGGTTATTCCAAGGCGCTCGACGCCTA
TCCGAAGTGGGACATCGCCAAGGCCAAGGCGCTGATGGCGGAAGCGGGTTATCCGAACGGCTTCACCGTGAAGATGAACT
GCCCGAACAACCGGTACATCAATGACGAGAAGATCTGCCAGGCCACCGTCGGCTTCCTCGGCCAGATCGGCATCAAGGCG
ACGCTGGATGCCCGCCCCATGGCCCAGCACACGCCCACCATCCTGAAGGACGAGACGGACTTCTACATGCTGGGCTGGGG
CGTTGCCTCGTTCGACGCCGCTTATACGCTCGACAGCATCTACCACAGCCGCGACAAGGTCTATGGCGCCTATAACGCCA
CCCGCGTCGTCAATCCCGAGCTCGACAAGAAGATCGAGGCGCTCGCAACCGAAGTGGACACCGCCAAGCGCAACGCGCTG
CTGGCGGAGGTTCTCGCGCAAGTGAAGGACGAGGCCTACTTCATCCCCATCCATAACCAGCTTCTTGCCTGGGGCATGAA
GAAGAACATCGACGTGAAGGTGCAGCCCGAGAACCAGCTGTTCGTGAAGTACGTCAAGATCGACTGA

Upstream 100 bases:

>100_bases
TGCGCAAAACCACCCTTGGAGGAATTGTAGCCCGAGGGAAAGCCAGTCTAGCATCCTGCGCCAACGATAAAACCAGAACC
TTCCGCTGGAGAATAGCTGA

Downstream 100 bases:

>100_bases
CCGGAAACGCGGCCGATCAGGACCTCCTGATCGGCCGCGCCGAATGCCGGGCCCGCGTGGGATCCGGCCTGCCTGCCTTC
CGGCCGCTCTCAGGGCGGCC

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MRRHFSTLLATVMVGLCLGAAADAKTFRWARGQDAPTLDPHTENSNQVFGLLHQFYEPLVQRDETGQLVPCLALSWELKP
SDPTVWIFKLRPNVTFHNGDKFTADDAVFSLKRAMDPLAGLRTTLAGVTDVTKVDDLTIEVKTAAPMPLLVNQITNVFMM
DKKWAEANNVARPQDFKNKEETFAVRNENGTGPYIVTSREPDSRTVMKLNDAYWGKGQFPLQATDVVYSVIASGPTRVAA
LLSGEVDFVQDMPVQDIDRVAQTPGLKVNTGAEVRSIFFGLNGGSPELPSSDVKGKNPLADKRVRQAIEIAINREAIQKV
VMGGQSAPSGTLIPPGVNGYSKALDAYPKWDIAKAKALMAEAGYPNGFTVKMNCPNNRYINDEKICQATVGFLGQIGIKA
TLDARPMAQHTPTILKDETDFYMLGWGVASFDAAYTLDSIYHSRDKVYGAYNATRVVNPELDKKIEALATEVDTAKRNAL
LAEVLAQVKDEAYFIPIHNQLLAWGMKKNIDVKVQPENQLFVKYVKID

Sequences:

>Translated_528_residues
MRRHFSTLLATVMVGLCLGAAADAKTFRWARGQDAPTLDPHTENSNQVFGLLHQFYEPLVQRDETGQLVPCLALSWELKP
SDPTVWIFKLRPNVTFHNGDKFTADDAVFSLKRAMDPLAGLRTTLAGVTDVTKVDDLTIEVKTAAPMPLLVNQITNVFMM
DKKWAEANNVARPQDFKNKEETFAVRNENGTGPYIVTSREPDSRTVMKLNDAYWGKGQFPLQATDVVYSVIASGPTRVAA
LLSGEVDFVQDMPVQDIDRVAQTPGLKVNTGAEVRSIFFGLNGGSPELPSSDVKGKNPLADKRVRQAIEIAINREAIQKV
VMGGQSAPSGTLIPPGVNGYSKALDAYPKWDIAKAKALMAEAGYPNGFTVKMNCPNNRYINDEKICQATVGFLGQIGIKA
TLDARPMAQHTPTILKDETDFYMLGWGVASFDAAYTLDSIYHSRDKVYGAYNATRVVNPELDKKIEALATEVDTAKRNAL
LAEVLAQVKDEAYFIPIHNQLLAWGMKKNIDVKVQPENQLFVKYVKID
>Mature_528_residues
MRRHFSTLLATVMVGLCLGAAADAKTFRWARGQDAPTLDPHTENSNQVFGLLHQFYEPLVQRDETGQLVPCLALSWELKP
SDPTVWIFKLRPNVTFHNGDKFTADDAVFSLKRAMDPLAGLRTTLAGVTDVTKVDDLTIEVKTAAPMPLLVNQITNVFMM
DKKWAEANNVARPQDFKNKEETFAVRNENGTGPYIVTSREPDSRTVMKLNDAYWGKGQFPLQATDVVYSVIASGPTRVAA
LLSGEVDFVQDMPVQDIDRVAQTPGLKVNTGAEVRSIFFGLNGGSPELPSSDVKGKNPLADKRVRQAIEIAINREAIQKV
VMGGQSAPSGTLIPPGVNGYSKALDAYPKWDIAKAKALMAEAGYPNGFTVKMNCPNNRYINDEKICQATVGFLGQIGIKA
TLDARPMAQHTPTILKDETDFYMLGWGVASFDAAYTLDSIYHSRDKVYGAYNATRVVNPELDKKIEALATEVDTAKRNAL
LAEVLAQVKDEAYFIPIHNQLLAWGMKKNIDVKVQPENQLFVKYVKID

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=511, Percent_Identity=24.26614481409, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789887, Length=524, Percent_Identity=24.8091603053435, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1789966, Length=511, Percent_Identity=26.2230919765166, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789397, Length=505, Percent_Identity=25.7425742574257, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787495, Length=526, Percent_Identity=23.574144486692, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87081878, Length=491, Percent_Identity=23.4215885947047, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787551, Length=552, Percent_Identity=22.2826086956522, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787762, Length=528, Percent_Identity=22.5378787878788, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58228; Mature: 58228

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRHFSTLLATVMVGLCLGAAADAKTFRWARGQDAPTLDPHTENSNQVFGLLHQFYEPLV
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QRDETGQLVPCLALSWELKPSDPTVWIFKLRPNVTFHNGDKFTADDAVFSLKRAMDPLAG
CCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LRTTLAGVTDVTKVDDLTIEVKTAAPMPLLVNQITNVFMMDKKWAEANNVARPQDFKNKE
HHHHHHCCCCCCEECCEEEEEECCCCCHHHHHHHHHHEEECCCHHCCCCCCCCCCCCCCC
ETFAVRNENGTGPYIVTSREPDSRTVMKLNDAYWGKGQFPLQATDVVYSVIASGPTRVAA
CEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHH
LLSGEVDFVQDMPVQDIDRVAQTPGLKVNTGAEVRSIFFGLNGGSPELPSSDVKGKNPLA
HHCCCCHHHHCCCHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCH
DKRVRQAIEIAINREAIQKVVMGGQSAPSGTLIPPGVNGYSKALDAYPKWDIAKAKALMA
HHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
EAGYPNGFTVKMNCPNNRYINDEKICQATVGFLGQIGIKATLDARPMAQHTPTILKDETD
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCEEECCCC
FYMLGWGVASFDAAYTLDSIYHSRDKVYGAYNATRVVNPELDKKIEALATEVDTAKRNAL
EEEEECCHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
LAEVLAQVKDEAYFIPIHNQLLAWGMKKNIDVKVQPENQLFVKYVKID
HHHHHHHHCCCEEEEEECHHHHHHCCCCCCEEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MRRHFSTLLATVMVGLCLGAAADAKTFRWARGQDAPTLDPHTENSNQVFGLLHQFYEPLV
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QRDETGQLVPCLALSWELKPSDPTVWIFKLRPNVTFHNGDKFTADDAVFSLKRAMDPLAG
CCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHH
LRTTLAGVTDVTKVDDLTIEVKTAAPMPLLVNQITNVFMMDKKWAEANNVARPQDFKNKE
HHHHHHCCCCCCEECCEEEEEECCCCCHHHHHHHHHHEEECCCHHCCCCCCCCCCCCCCC
ETFAVRNENGTGPYIVTSREPDSRTVMKLNDAYWGKGQFPLQATDVVYSVIASGPTRVAA
CEEEEECCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHH
LLSGEVDFVQDMPVQDIDRVAQTPGLKVNTGAEVRSIFFGLNGGSPELPSSDVKGKNPLA
HHCCCCHHHHCCCHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCH
DKRVRQAIEIAINREAIQKVVMGGQSAPSGTLIPPGVNGYSKALDAYPKWDIAKAKALMA
HHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
EAGYPNGFTVKMNCPNNRYINDEKICQATVGFLGQIGIKATLDARPMAQHTPTILKDETD
HCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCEEECCCC
FYMLGWGVASFDAAYTLDSIYHSRDKVYGAYNATRVVNPELDKKIEALATEVDTAKRNAL
EEEEECCHHHHHHHHHHHHHHHHHHHEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
LAEVLAQVKDEAYFIPIHNQLLAWGMKKNIDVKVQPENQLFVKYVKID
HHHHHHHHCCCEEEEEECHHHHHHCCCCCCEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA