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The map label for this gene is potH [H]

Identifier: 158423594

GI number: 158423594

Start: 2272079

End: 2273011

Strand: Reverse

Name: potH [H]

Synonym: AZC_1970

Alternate gene names: 158423594

Gene position: 2273011-2272079 (Counterclockwise)

Preceding gene: 158423595

Following gene: 158423593

Centisome position: 42.33

GC content: 65.38

Gene sequence:

>933_bases
ATGCGACCGCGTGCGCCCCTCACTCTCCGCCGCGCCAGTGGCGGTCAGTCCGAAGCCCTTTCGAAACCAAGGCCCATTGC
CATGGCGACCACCGCGCCCACCGCCTATGACGTGAAACTCGCCTTTCCACTGGGCGCGTTTCTGGCCGTCTTCTTCCTCG
CGCCGCTGGCGGTGCTGCTGGTGCTGAGCTTTCAAGTGGACCCCAAGGGCACCGCCTATGGGCTCAACCAGTATGGCCAG
TTTCTGGGCGATGCCTTCAGCCTCGGCGTGCTGGGCAACACTCTGCTGCTCGGCCTCAAGGTCACGGCGCTGTGCCTGCT
CATGGGCTATCCGGTGGCCTATGCCTATACGCGCGTCGGGCCGCGACTGAAGAGCCTCATCATCCTCATCGTGCTGATGC
CGCTCCTCACCTCCGTGGTGGTGCGCACCTTTGCCTGGATCGTCATTCTGGGGCGGCAGGGCATCGTCAATTCCGCCCTC
ATGTCCATGGGGATCATCTCGACGCCGCTGCGCCTCATCTATACCGACCTCGGCCTCGTGGTGGCGCTGGCGCAGGTGCA
GATGCCGCTCATGGTGCTGCCGCTCATCACCGCTATCGGGCGTATCGACCCGAACCTCACGGACGCCTCGCTCTCGCTGG
GCTCCGGGGCCTGGCGCACCTTCCTGCGGGTGACGCTGCCGCTGTCGCTGCCGGGCATCATCGCCGGCTCCATGCTCACC
TATGCCGCGGCCATCACCGCCTTCATCACCCAGAGCCTCGTGGGCGGCGGGCAGATGCTGTTCATGCCCATGTACATCTA
TCAGCAGGCCTCCACGCTCCAGAACTGGCCGTTCGCGGCCGCCATTTCCATCATCTTCCTCGTCGCCGTGCTGGCGGTGG
TGATGGTGTTCAACCTGCTCGGCCGCCTGTCGCGCGGCTATGCCAGCACCTGA

Upstream 100 bases:

>100_bases
GCCATGCGCGGAGGTGTCCTATCAACCGCCGCCCGTCGCGCCGGCGGCCCGGCGTGGATGCCCGGGACGAGCCCGGGCAT
GACGAACCCGAGGGCCGCAC

Downstream 100 bases:

>100_bases
GGACGAGCCATGCGGCGCGACTGGGAAACCTTCTCCTTCCACCTCGTCATCGGCCTCATCGCGGTGCTGGCGCTGATCCT
GCTGGTGGCCCCGACGGCGA

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRPRAPLTLRRASGGQSEALSKPRPIAMATTAPTAYDVKLAFPLGAFLAVFFLAPLAVLLVLSFQVDPKGTAYGLNQYGQ
FLGDAFSLGVLGNTLLLGLKVTALCLLMGYPVAYAYTRVGPRLKSLIILIVLMPLLTSVVVRTFAWIVILGRQGIVNSAL
MSMGIISTPLRLIYTDLGLVVALAQVQMPLMVLPLITAIGRIDPNLTDASLSLGSGAWRTFLRVTLPLSLPGIIAGSMLT
YAAAITAFITQSLVGGGQMLFMPMYIYQQASTLQNWPFAAAISIIFLVAVLAVVMVFNLLGRLSRGYAST

Sequences:

>Translated_310_residues
MRPRAPLTLRRASGGQSEALSKPRPIAMATTAPTAYDVKLAFPLGAFLAVFFLAPLAVLLVLSFQVDPKGTAYGLNQYGQ
FLGDAFSLGVLGNTLLLGLKVTALCLLMGYPVAYAYTRVGPRLKSLIILIVLMPLLTSVVVRTFAWIVILGRQGIVNSAL
MSMGIISTPLRLIYTDLGLVVALAQVQMPLMVLPLITAIGRIDPNLTDASLSLGSGAWRTFLRVTLPLSLPGIIAGSMLT
YAAAITAFITQSLVGGGQMLFMPMYIYQQASTLQNWPFAAAISIIFLVAVLAVVMVFNLLGRLSRGYAST
>Mature_310_residues
MRPRAPLTLRRASGGQSEALSKPRPIAMATTAPTAYDVKLAFPLGAFLAVFFLAPLAVLLVLSFQVDPKGTAYGLNQYGQ
FLGDAFSLGVLGNTLLLGLKVTALCLLMGYPVAYAYTRVGPRLKSLIILIVLMPLLTSVVVRTFAWIVILGRQGIVNSAL
MSMGIISTPLRLIYTDLGLVVALAQVQMPLMVLPLITAIGRIDPNLTDASLSLGSGAWRTFLRVTLPLSLPGIIAGSMLT
YAAAITAFITQSLVGGGQMLFMPMYIYQQASTLQNWPFAAAISIIFLVAVLAVVMVFNLLGRLSRGYAST

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=233, Percent_Identity=33.4763948497854, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1787369, Length=213, Percent_Identity=31.924882629108, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787713, Length=207, Percent_Identity=29.4685990338164, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=30.4932735426009, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33083; Mature: 33083

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRAPLTLRRASGGQSEALSKPRPIAMATTAPTAYDVKLAFPLGAFLAVFFLAPLAVLL
CCCCCCEEEECCCCCCHHHCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
VLSFQVDPKGTAYGLNQYGQFLGDAFSLGVLGNTLLLGLKVTALCLLMGYPVAYAYTRVG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHC
PRLKSLIILIVLMPLLTSVVVRTFAWIVILGRQGIVNSALMSMGIISTPLRLIYTDLGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VALAQVQMPLMVLPLITAIGRIDPNLTDASLSLGSGAWRTFLRVTLPLSLPGIIAGSMLT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
YAAAITAFITQSLVGGGQMLFMPMYIYQQASTLQNWPFAAAISIIFLVAVLAVVMVFNLL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GRLSRGYAST
HHHHCCCCCC
>Mature Secondary Structure
MRPRAPLTLRRASGGQSEALSKPRPIAMATTAPTAYDVKLAFPLGAFLAVFFLAPLAVLL
CCCCCCEEEECCCCCCHHHCCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
VLSFQVDPKGTAYGLNQYGQFLGDAFSLGVLGNTLLLGLKVTALCLLMGYPVAYAYTRVG
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHC
PRLKSLIILIVLMPLLTSVVVRTFAWIVILGRQGIVNSALMSMGIISTPLRLIYTDLGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VALAQVQMPLMVLPLITAIGRIDPNLTDASLSLGSGAWRTFLRVTLPLSLPGIIAGSMLT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
YAAAITAFITQSLVGGGQMLFMPMYIYQQASTLQNWPFAAAISIIFLVAVLAVVMVFNLL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GRLSRGYAST
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]