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The map label for this gene is ltaA [H]

Identifier: 158423547

GI number: 158423547

Start: 2225895

End: 2226968

Strand: Reverse

Name: ltaA [H]

Synonym: AZC_1923

Alternate gene names: 158423547

Gene position: 2226968-2225895 (Counterclockwise)

Preceding gene: 158423548

Following gene: 158423542

Centisome position: 41.47

GC content: 68.81

Gene sequence:

>1074_bases
ATGTCGGTCCAAGAGAATGTCGCGGGAACGCTGGCGCGGCCGTCCGCAGATCAGGCGCCCGTGGATCTGCGCAGCGACAC
CGTGACCCGTCCCACCGCCGCCATGTACGAGCGGATGATGGCTGCCGAAGTGGGTGATGATGGCCTCGACGGCGACCCCA
CGGCCCGCGAGCTGGAGGCCTATACGGCCCAGACCTTGGGCAAGGAGGCGGGCCTGTTCGTGCCGAGCTGCACCATGGCC
AATCTCCTTGCAATTCTCGCGCAGGCGGAGCGTGGCGAGCAGGTGGTGCTGGAAAGCTCCGCCCACATCTACACCTCTGA
ACGGGGATCGGGCACCTTGACCGGGCTGTTCTATCTCCCGGTTCCCGGCACGGCCGGCGCGATGGATGTCAACGCGCTTG
AAGACGCGCTCACCGCCACCGGCCATCGCCTGCGCACCGCGCTCGTGGGCATGGAAACCTCCCACAACAATGCCGGCGGC
GCCGTGCTCCCGCTCGCGCACATGCGCACCGTCCACGACCTCGCCCATGCCCGTGGCGCGCGCGTGCACCTCGACGGCGC
GCGTCTGTTCAACGCCGCCGTGGCGCTGGATGTGCGCCCGGCGGAGATCGCGCAGTTCGCCGACACCGTGTCGCTGTGCC
TGTCCAAGGGCCTGTCTGCACCGGTGGGGGCCATCCTCGCCGGCCCGAGGGACACCATCGCGAAGTGCCGCCCCCTGCGG
CGGATGCTCGGCGGCGCACAGCGGCAGATTGGCATCATGGCCGCGGCCGGGCTTGAAGCCGTGCGCACCATGGGACCGCG
CCTCGCCGAGGACCATCGCCGGGCCCGGCGCCTCAGCGAGGGCGTCAATGCGCTGGCCGGCACGATTTCGGCGAGCAGCC
CACAGACGAACATCATCCAGATCGACGTGTCGCGATCCGGCCGCGACAGCGGGGCGTGGGTCCAGCGTCTGGAAGGCATC
GGCCTCCTCACCCGGCCGTGGGGAAAACAAAAATTACGTTGCGTCACGCATCGCGAGATCGACGATGCCGCCATTGAACG
CGCCATCGCCGCCTTCGCAAAAGCTCTCGTCTAG

Upstream 100 bases:

>100_bases
TGAGGACTGCCCCAAGAGCGAATTCTTCGACCATGTCGAGGCCCGGCACGAGCGGACCCGACTGTTCCTTTCCAAGATTC
TCAAGCAGTAGGGTGCCACG

Downstream 100 bases:

>100_bases
GAGACAACGCGACCCTTCAAACGCTGAGGGCTGCCCCCTACACCGCAAGAGGCAATGCTCGTTCCAGAGCAAACACCAAA
TCAGAGTATGTGAAGCGAAG

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSVQENVAGTLARPSADQAPVDLRSDTVTRPTAAMYERMMAAEVGDDGLDGDPTARELEAYTAQTLGKEAGLFVPSCTMA
NLLAILAQAERGEQVVLESSAHIYTSERGSGTLTGLFYLPVPGTAGAMDVNALEDALTATGHRLRTALVGMETSHNNAGG
AVLPLAHMRTVHDLAHARGARVHLDGARLFNAAVALDVRPAEIAQFADTVSLCLSKGLSAPVGAILAGPRDTIAKCRPLR
RMLGGAQRQIGIMAAAGLEAVRTMGPRLAEDHRRARRLSEGVNALAGTISASSPQTNIIQIDVSRSGRDSGAWVQRLEGI
GLLTRPWGKQKLRCVTHREIDDAAIERAIAAFAKALV

Sequences:

>Translated_357_residues
MSVQENVAGTLARPSADQAPVDLRSDTVTRPTAAMYERMMAAEVGDDGLDGDPTARELEAYTAQTLGKEAGLFVPSCTMA
NLLAILAQAERGEQVVLESSAHIYTSERGSGTLTGLFYLPVPGTAGAMDVNALEDALTATGHRLRTALVGMETSHNNAGG
AVLPLAHMRTVHDLAHARGARVHLDGARLFNAAVALDVRPAEIAQFADTVSLCLSKGLSAPVGAILAGPRDTIAKCRPLR
RMLGGAQRQIGIMAAAGLEAVRTMGPRLAEDHRRARRLSEGVNALAGTISASSPQTNIIQIDVSRSGRDSGAWVQRLEGI
GLLTRPWGKQKLRCVTHREIDDAAIERAIAAFAKALV
>Mature_356_residues
SVQENVAGTLARPSADQAPVDLRSDTVTRPTAAMYERMMAAEVGDDGLDGDPTARELEAYTAQTLGKEAGLFVPSCTMAN
LLAILAQAERGEQVVLESSAHIYTSERGSGTLTGLFYLPVPGTAGAMDVNALEDALTATGHRLRTALVGMETSHNNAGGA
VLPLAHMRTVHDLAHARGARVHLDGARLFNAAVALDVRPAEIAQFADTVSLCLSKGLSAPVGAILAGPRDTIAKCRPLRR
MLGGAQRQIGIMAAAGLEAVRTMGPRLAEDHRRARRLSEGVNALAGTISASSPQTNIIQIDVSRSGRDSGAWVQRLEGIG
LLTRPWGKQKLRCVTHREIDDAAIERAIAAFAKALV

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=264, Percent_Identity=43.1818181818182, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI193206522, Length=364, Percent_Identity=36.5384615384615, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI193206520, Length=364, Percent_Identity=36.5384615384615, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI193206524, Length=364, Percent_Identity=36.5384615384615, Blast_Score=210, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6320789, Length=352, Percent_Identity=30.6818181818182, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355439, Length=367, Percent_Identity=39.5095367847411, Blast_Score=210, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 37646; Mature: 37515

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVQENVAGTLARPSADQAPVDLRSDTVTRPTAAMYERMMAAEVGDDGLDGDPTARELEA
CCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YTAQTLGKEAGLFVPSCTMANLLAILAQAERGEQVVLESSAHIYTSERGSGTLTGLFYLP
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCEEEEEEEEE
VPGTAGAMDVNALEDALTATGHRLRTALVGMETSHNNAGGAVLPLAHMRTVHDLAHARGA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCC
RVHLDGARLFNAAVALDVRPAEIAQFADTVSLCLSKGLSAPVGAILAGPRDTIAKCRPLR
EEEECHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHH
RMLGGAQRQIGIMAAAGLEAVRTMGPRLAEDHRRARRLSEGVNALAGTISASSPQTNIIQ
HHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
IDVSRSGRDSGAWVQRLEGIGLLTRPWGKQKLRCVTHREIDDAAIERAIAAFAKALV
EEECCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVQENVAGTLARPSADQAPVDLRSDTVTRPTAAMYERMMAAEVGDDGLDGDPTARELEA
CCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YTAQTLGKEAGLFVPSCTMANLLAILAQAERGEQVVLESSAHIYTSERGSGTLTGLFYLP
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCEEEEEEEEE
VPGTAGAMDVNALEDALTATGHRLRTALVGMETSHNNAGGAVLPLAHMRTVHDLAHARGA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCC
RVHLDGARLFNAAVALDVRPAEIAQFADTVSLCLSKGLSAPVGAILAGPRDTIAKCRPLR
EEEECHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHH
RMLGGAQRQIGIMAAAGLEAVRTMGPRLAEDHRRARRLSEGVNALAGTISASSPQTNIIQ
HHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
IDVSRSGRDSGAWVQRLEGIGLLTRPWGKQKLRCVTHREIDDAAIERAIAAFAKALV
EEECCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]