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The map label for this gene is citE [H]

Identifier: 158423465

GI number: 158423465

Start: 2106118

End: 2106927

Strand: Reverse

Name: citE [H]

Synonym: AZC_1841

Alternate gene names: 158423465

Gene position: 2106927-2106118 (Counterclockwise)

Preceding gene: 158423469

Following gene: 158423464

Centisome position: 39.24

GC content: 71.48

Gene sequence:

>810_bases
ATGACCACCTTCCTTCATACCGAGCGCCTCGTGGCGCTCTTCGTGCCCGGTGATCGGCCGGAACGCTTCGGCAAGGCGCT
CGCCGCCGGGGCCGATGCGGTGATCGTCGATCTGGAGGATGCGGTGGCGCCCGGCGACAAGGGCAAGGCCCGCGACGCCA
TCGCCGCCCACTGGCCGACAGAGGGCCGGCTTCTGCTGCGCATCAATGGCTGCGGCACCCCCTTCCTCGCGGACGACCTC
GCCCTCGCCCGCCGGCTGCGCCTCAGCGGCATCATCCTGCCCAAGGCCGAGCAGGCGCAGAACATCTCGACCGTCCATGC
GGCCGTGCCGGACGTGCCGGTGATCGCGCTCATCGAGAGCGCGCTGGGCCTTGCCAATGCGCGCGCGCTCGCGGCCACCC
ATGGGGTCGCCCGGCTCGCCTTCGGCTCCATCGACTATTGCGCCGATCTCGGCAGCAGCCACAGCCGCGAGGCCTTGCTG
ATGGCGCGCTCGGAACTCGTGCTCGCCTCCCGCCTTGCCCGCCGCGCCGGCCCCATCGACGGCGTCACGGCGACGGTCGC
GGACGCAACGCTGGCGCGGGACGATGCCGCTTATGCGGCCGGGCTCGGCTTCGCCGGCAAGCTGTGCATCCATCCGAGCC
AGATCGCGCCGGTGCTGGCCGGCTTTGCCCCCAGCCCCTCGGACATCGCATGGGCCCAGCGCATCCTCGCTGCGGCATCC
GAAGGGGCGGTGAAGGTGGATGGCGCCATGGTGGATGCGCCGGTCCGGATTCGGGCCGAGCAGATCCTGAAGCGTGCGGA
GATCGGCTGA

Upstream 100 bases:

>100_bases
GCGCCCATAGGACTTTGCGATGGCGGCGCTTTGATTTTGCTATTTGCCCCGGCGTCCGCGCACGGCACTTTAGGCGCCGT
CCGACAGCGGGGGGCTGCCC

Downstream 100 bases:

>100_bases
GCGGCAGGCATTTCGCGTCGGCCGCGCTCCCGTGGCATGGTGGCGCCGCCCGCACCGGCGGGCGGTCCGGAGTTCCCATG
CGCCTCGCCCGTATCCTTGC

Product: citrate lyase beta chain

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTTFLHTERLVALFVPGDRPERFGKALAAGADAVIVDLEDAVAPGDKGKARDAIAAHWPTEGRLLLRINGCGTPFLADDL
ALARRLRLSGIILPKAEQAQNISTVHAAVPDVPVIALIESALGLANARALAATHGVARLAFGSIDYCADLGSSHSREALL
MARSELVLASRLARRAGPIDGVTATVADATLARDDAAYAAGLGFAGKLCIHPSQIAPVLAGFAPSPSDIAWAQRILAAAS
EGAVKVDGAMVDAPVRIRAEQILKRAEIG

Sequences:

>Translated_269_residues
MTTFLHTERLVALFVPGDRPERFGKALAAGADAVIVDLEDAVAPGDKGKARDAIAAHWPTEGRLLLRINGCGTPFLADDL
ALARRLRLSGIILPKAEQAQNISTVHAAVPDVPVIALIESALGLANARALAATHGVARLAFGSIDYCADLGSSHSREALL
MARSELVLASRLARRAGPIDGVTATVADATLARDDAAYAAGLGFAGKLCIHPSQIAPVLAGFAPSPSDIAWAQRILAAAS
EGAVKVDGAMVDAPVRIRAEQILKRAEIG
>Mature_268_residues
TTFLHTERLVALFVPGDRPERFGKALAAGADAVIVDLEDAVAPGDKGKARDAIAAHWPTEGRLLLRINGCGTPFLADDLA
LARRLRLSGIILPKAEQAQNISTVHAAVPDVPVIALIESALGLANARALAATHGVARLAFGSIDYCADLGSSHSREALLM
ARSELVLASRLARRAGPIDGVTATVADATLARDDAAYAAGLGFAGKLCIHPSQIAPVLAGFAPSPSDIAWAQRILAAASE
GAVKVDGAMVDAPVRIRAEQILKRAEIG

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=293, Percent_Identity=29.0102389078498, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI87081764, Length=288, Percent_Identity=31.25, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557458, Length=301, Percent_Identity=29.5681063122924, Blast_Score=101, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 27879; Mature: 27748

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFLHTERLVALFVPGDRPERFGKALAAGADAVIVDLEDAVAPGDKGKARDAIAAHWPT
CCCEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCEEEECCC
EGRLLLRINGCGTPFLADDLALARRLRLSGIILPKAEQAQNISTVHAAVPDVPVIALIES
CCEEEEEECCCCCCHHHHHHHHHHHHHHCEEECCCCHHHCCCHHHHHHCCCCHHHHHHHH
ALGLANARALAATHGVARLAFGSIDYCADLGSSHSREALLMARSELVLASRLARRAGPID
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GVTATVADATLARDDAAYAAGLGFAGKLCIHPSQIAPVLAGFAPSPSDIAWAQRILAAAS
CCCHHHHHHHHHCCCHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
EGAVKVDGAMVDAPVRIRAEQILKRAEIG
CCCEEECCEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTFLHTERLVALFVPGDRPERFGKALAAGADAVIVDLEDAVAPGDKGKARDAIAAHWPT
CCEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCEEEECCC
EGRLLLRINGCGTPFLADDLALARRLRLSGIILPKAEQAQNISTVHAAVPDVPVIALIES
CCEEEEEECCCCCCHHHHHHHHHHHHHHCEEECCCCHHHCCCHHHHHHCCCCHHHHHHHH
ALGLANARALAATHGVARLAFGSIDYCADLGSSHSREALLMARSELVLASRLARRAGPID
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GVTATVADATLARDDAAYAAGLGFAGKLCIHPSQIAPVLAGFAPSPSDIAWAQRILAAAS
CCCHHHHHHHHHCCCHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
EGAVKVDGAMVDAPVRIRAEQILKRAEIG
CCCEEECCEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]