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The map label for this gene is phbC

Identifier: 158423469

GI number: 158423469

Start: 2109918

End: 2111669

Strand: Reverse

Name: phbC

Synonym: AZC_1845

Alternate gene names: 158423469

Gene position: 2111669-2109918 (Counterclockwise)

Preceding gene: 158423476

Following gene: 158423465

Centisome position: 39.33

GC content: 63.64

Gene sequence:

>1752_bases
ATGGAGGCGTTCGCCCAGAACCTTGCGAAGATGGTGGAGGAAGGCGGCAAGGCCGTCGCCGCTTACATGCGTCCGCGCGA
GGAGGGCAAGCCGGACGACATGACGGACGATATCGCCGATGCGCTCAAGACCATCGGCGAGGTGGCCAATTACTGGATGT
CCGATCCAAAGCGCTCCTTCGAGGCCCAGTCGCGCCTCATGATGGGCTACATGGGCGTCTGGGCGGGGGCGCTCCAGAAG
CTCTCCGGCGAGAAGGCCGAGCCCATCGCGAAGGCCGACCCCAAGGACGGCCGCTTCAAGGACCCGGAATGGGAAAGCCC
GTTCTTCGACGCCCTGAAGCAGACCTATCTCGTGACCAGCAACTGGGCCGAGTCCATGGTCAAGGAGGCCGAGGGGCTCG
ATCCCCACACCAAGCACAAGGCCGAATTCCTGGTGCGCCAGCTCTCGAATGCGGTGGCGCCCTCGAACTTCCTCATGACC
AACCCGGAGCTGATCCGCGAAACGCTCTCCTCCAGCGGCGAGAACCTCGTGCGCGGCATGAAGAATCTGGCCGAGGATCT
GGTGGAGGGCAAAGGCGATCTCAAGATCCGCCAGACGGACATGAGCGCCTTCGAGGTGGGCCGCAATCTGGCGCTCAGCC
CCGGCAAGGTCATCTTCGAGACCGAGCTGATGCAGCTCATCCAGTATGCGCCCTCGACGCCCAGCGTGAAGAAGACGCCG
GTGCTGATCGTGCCGCCCTGGATCAACAAGTTCTACATCCTCGACCTGACGCCCGAGAAATCCCTCATCAAGTGGATGGT
GGATCAGGGGCTGACGGTCTTCGTCATCTCGTGGGTCAATCCGGACGCCCGTCTCGCCGACAAGGGCTTCGACGACTACA
TGCGCGATGGCATCTTCGCCGCGCTCGATGCGGTGGAGAAGGCGACCGGCGAGCATCAGGCGCACACCATCGGCTATTGC
GTGGGCGGCACGCTGCTCGCGGTCACGCTTGCCTACATGGCGGCGACCGGCGATGACCGGGTGGCGAGCTCCACCTTCCT
CACCACCCAGATCGACTTCACCCACGCGGGCGATCTCAAGGTCTTCGTGGACGAGGCGCAGCTCTCGGTCATCGAGCGTC
GCATGAAGGAGATGGGCTATCTTGAAGGACGCAAGATGGCCGACGCCTTCAACATGCTGCGGTCCAACGACCTGATCTGG
CCCTATGTGGTGAACAATTATCTCAAGGGGAAGCAGCCGTTCCCCTTCGATCTTCTGTTCTGGAATGCCGATTCCACCCG
CATGCCGGCGGCGAACCACTCCTATTACCTGCGCAACTGCTATCTCCAGAACAACATCGCCAAGGGGCTGGCGGAGATCG
CGGGCGTCAAGATCGACATGGGCAAGGTGACGATCCCGGTCTATTCGCTCGCCACCCGCGAGGACCATATCGCGCCGCCC
AACTCCGCTTATATCGGTGCAGGCCTGCTCGGCGGCCCCGTGCGCTTCGTGCTGGCCGGGTCCGGCCATATTGCGGGCGT
GGTCAATCCGCCGGTGAAGCACAAGTACCAGTACTGGACCGGCGGTCCGACCGGCGGGGACTATGACGTCTGGCTGAAGG
GGGCGCAGGAGCACAAGGGCTCGTGGTGGCCGGATTGGGCGCAGTGGTTCAGCGCCCTGCATCCGGACGAGGTCCCTGCC
CGCGAGCCAGGCGGCAGCGCGTTCAATCCCATCGAGGATGCCCCCGGCCGCTACGTACGCGAGAAGTCCTGA

Upstream 100 bases:

>100_bases
CCGTCGGGAAAACGATCTATAAAGGACGGAATCGTCTCAAGGAATCCCCATGGCCTCGCCGAGCGACGTGACCACGACAG
AGCGCCCTGCCTCCGTAAAC

Downstream 100 bases:

>100_bases
GCCGCAGGCTGTCACCTGCCTTAATCGGCTAGCGTCGCCGGCCATGTGCCGTCGCGCACCTGCTGCTGAAGGATGGTGAG
CGCCAGTTGCTCCACCGCCC

Product: poly-beta-hydroxybutyrate polymerase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MEAFAQNLAKMVEEGGKAVAAYMRPREEGKPDDMTDDIADALKTIGEVANYWMSDPKRSFEAQSRLMMGYMGVWAGALQK
LSGEKAEPIAKADPKDGRFKDPEWESPFFDALKQTYLVTSNWAESMVKEAEGLDPHTKHKAEFLVRQLSNAVAPSNFLMT
NPELIRETLSSSGENLVRGMKNLAEDLVEGKGDLKIRQTDMSAFEVGRNLALSPGKVIFETELMQLIQYAPSTPSVKKTP
VLIVPPWINKFYILDLTPEKSLIKWMVDQGLTVFVISWVNPDARLADKGFDDYMRDGIFAALDAVEKATGEHQAHTIGYC
VGGTLLAVTLAYMAATGDDRVASSTFLTTQIDFTHAGDLKVFVDEAQLSVIERRMKEMGYLEGRKMADAFNMLRSNDLIW
PYVVNNYLKGKQPFPFDLLFWNADSTRMPAANHSYYLRNCYLQNNIAKGLAEIAGVKIDMGKVTIPVYSLATREDHIAPP
NSAYIGAGLLGGPVRFVLAGSGHIAGVVNPPVKHKYQYWTGGPTGGDYDVWLKGAQEHKGSWWPDWAQWFSALHPDEVPA
REPGGSAFNPIEDAPGRYVREKS

Sequences:

>Translated_583_residues
MEAFAQNLAKMVEEGGKAVAAYMRPREEGKPDDMTDDIADALKTIGEVANYWMSDPKRSFEAQSRLMMGYMGVWAGALQK
LSGEKAEPIAKADPKDGRFKDPEWESPFFDALKQTYLVTSNWAESMVKEAEGLDPHTKHKAEFLVRQLSNAVAPSNFLMT
NPELIRETLSSSGENLVRGMKNLAEDLVEGKGDLKIRQTDMSAFEVGRNLALSPGKVIFETELMQLIQYAPSTPSVKKTP
VLIVPPWINKFYILDLTPEKSLIKWMVDQGLTVFVISWVNPDARLADKGFDDYMRDGIFAALDAVEKATGEHQAHTIGYC
VGGTLLAVTLAYMAATGDDRVASSTFLTTQIDFTHAGDLKVFVDEAQLSVIERRMKEMGYLEGRKMADAFNMLRSNDLIW
PYVVNNYLKGKQPFPFDLLFWNADSTRMPAANHSYYLRNCYLQNNIAKGLAEIAGVKIDMGKVTIPVYSLATREDHIAPP
NSAYIGAGLLGGPVRFVLAGSGHIAGVVNPPVKHKYQYWTGGPTGGDYDVWLKGAQEHKGSWWPDWAQWFSALHPDEVPA
REPGGSAFNPIEDAPGRYVREKS
>Mature_583_residues
MEAFAQNLAKMVEEGGKAVAAYMRPREEGKPDDMTDDIADALKTIGEVANYWMSDPKRSFEAQSRLMMGYMGVWAGALQK
LSGEKAEPIAKADPKDGRFKDPEWESPFFDALKQTYLVTSNWAESMVKEAEGLDPHTKHKAEFLVRQLSNAVAPSNFLMT
NPELIRETLSSSGENLVRGMKNLAEDLVEGKGDLKIRQTDMSAFEVGRNLALSPGKVIFETELMQLIQYAPSTPSVKKTP
VLIVPPWINKFYILDLTPEKSLIKWMVDQGLTVFVISWVNPDARLADKGFDDYMRDGIFAALDAVEKATGEHQAHTIGYC
VGGTLLAVTLAYMAATGDDRVASSTFLTTQIDFTHAGDLKVFVDEAQLSVIERRMKEMGYLEGRKMADAFNMLRSNDLIW
PYVVNNYLKGKQPFPFDLLFWNADSTRMPAANHSYYLRNCYLQNNIAKGLAEIAGVKIDMGKVTIPVYSLATREDHIAPP
NSAYIGAGLLGGPVRFVLAGSGHIAGVVNPPVKHKYQYWTGGPTGGDYDVWLKGAQEHKGSWWPDWAQWFSALHPDEVPA
REPGGSAFNPIEDAPGRYVREKS

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHBC_AZOC5 (O66392)

Other databases:

- EMBL:   AJ006237
- EMBL:   AP009384
- RefSeq:   YP_001524761.1
- ProteinModelPortal:   O66392
- GeneID:   5691865
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1845
- HOGENOM:   HBG294095
- OMA:   IYNLATR
- ProtClustDB:   CLSK980042
- BioCyc:   ACAU438753:AZC_1845-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941
- TIGRFAMs:   TIGR01838

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N

EC number: NA

Molecular weight: Translated: 64740; Mature: 64740

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: ACT_SITE 320-320

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFAQNLAKMVEEGGKAVAAYMRPREEGKPDDMTDDIADALKTIGEVANYWMSDPKRSF
CCHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
EAQSRLMMGYMGVWAGALQKLSGEKAEPIAKADPKDGRFKDPEWESPFFDALKQTYLVTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEC
NWAESMVKEAEGLDPHTKHKAEFLVRQLSNAVAPSNFLMTNPELIRETLSSSGENLVRGM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCHHHHHHH
KNLAEDLVEGKGDLKIRQTDMSAFEVGRNLALSPGKVIFETELMQLIQYAPSTPSVKKTP
HHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCC
VLIVPPWINKFYILDLTPEKSLIKWMVDQGLTVFVISWVNPDARLADKGFDDYMRDGIFA
EEEECCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHH
ALDAVEKATGEHQAHTIGYCVGGTLLAVTLAYMAATGDDRVASSTFLTTQIDFTHAGDLK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEE
VFVDEAQLSVIERRMKEMGYLEGRKMADAFNMLRSNDLIWPYVVNNYLKGKQPFPFDLLF
EEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCEEEEE
WNADSTRMPAANHSYYLRNCYLQNNIAKGLAEIAGVKIDMGKVTIPVYSLATREDHIAPP
ECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCEEECCEEEEEEEEEECCCCCCCCC
NSAYIGAGLLGGPVRFVLAGSGHIAGVVNPPVKHKYQYWTGGPTGGDYDVWLKGAQEHKG
CCCEECCCCCCCCEEEEEECCCCEEECCCCCHHCCEEEECCCCCCCCHHEEECCCHHCCC
SWWPDWAQWFSALHPDEVPAREPGGSAFNPIEDAPGRYVREKS
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEAFAQNLAKMVEEGGKAVAAYMRPREEGKPDDMTDDIADALKTIGEVANYWMSDPKRSF
CCHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
EAQSRLMMGYMGVWAGALQKLSGEKAEPIAKADPKDGRFKDPEWESPFFDALKQTYLVTS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEC
NWAESMVKEAEGLDPHTKHKAEFLVRQLSNAVAPSNFLMTNPELIRETLSSSGENLVRGM
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCHHHHHHH
KNLAEDLVEGKGDLKIRQTDMSAFEVGRNLALSPGKVIFETELMQLIQYAPSTPSVKKTP
HHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCC
VLIVPPWINKFYILDLTPEKSLIKWMVDQGLTVFVISWVNPDARLADKGFDDYMRDGIFA
EEEECCCCCCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHH
ALDAVEKATGEHQAHTIGYCVGGTLLAVTLAYMAATGDDRVASSTFLTTQIDFTHAGDLK
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEE
VFVDEAQLSVIERRMKEMGYLEGRKMADAFNMLRSNDLIWPYVVNNYLKGKQPFPFDLLF
EEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCEEEEE
WNADSTRMPAANHSYYLRNCYLQNNIAKGLAEIAGVKIDMGKVTIPVYSLATREDHIAPP
ECCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCEEECCEEEEEEEEEECCCCCCCCC
NSAYIGAGLLGGPVRFVLAGSGHIAGVVNPPVKHKYQYWTGGPTGGDYDVWLKGAQEHKG
CCCEECCCCCCCCEEEEEECCCCEEECCCCCHHCCEEEECCCCCCCCHHEEECCCHHCCC
SWWPDWAQWFSALHPDEVPAREPGGSAFNPIEDAPGRYVREKS
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438