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The map label for this gene is proS

Identifier: 158423310

GI number: 158423310

Start: 1928192

End: 1929514

Strand: Direct

Name: proS

Synonym: AZC_1686

Alternate gene names: 158423310

Gene position: 1928192-1929514 (Clockwise)

Preceding gene: 158423309

Following gene: 158423311

Centisome position: 35.91

GC content: 65.08

Gene sequence:

>1323_bases
ATGCGCCTGTCCCGCTACTTCCTGCCCATTCTCCGCGAGGTTCCGAAGGAAGCGGACATCGTCTCGCACCGCCTCATGCT
GCGCGCCGGCATGATCCGGCAGGAGAGCGCCGGCATCTATGCCTGGCTGCCGCTGGGCCTGCGCGTGCTGAACAAGATCT
GCAACATCATCCGCGAGGAGCAGAATCGGACCGGCGCGGTCGAACTGCTGATGCCCACCATCCAGTCCGCGGACCTGTGG
CGCGAGAGCGGCCGCTATGACGACTATGGCAAGGAGATGCTGCGCATCAAGGACCGGCACGAGCGCGACATGCTGTTCGG
GCCGACCAACGAGGAGATGATCACCGAGATCGTCCGCGGCTCCATCAAGTCCTACAAGAGCCTGCCGCTGAACCTCTACC
ACATCCAGTGGAAGTTCCGGGACGAGGTGCGCCCGCGCTTCGGCGTCTACCGCTCGCGTGAATTCCTGATGAAGGACGCC
TATTCCTTCGATCTGGACGCGGAGGGCGCCAAGCACTCCTACAACAAGATGTTCGTCGCCTATCTGCGCACCTTCGCGCG
CATGGGCCTCAAAGCCATCCCCATGGTGGCCGACACCGGCCCCATCGGCGGCAACCTCTCGCACGAATTCATCATTCTCG
CCTCCACCGGCGAGAGCGAGGTGTTCTGCCATGGCGACTATCTGGAGATGGCGCCCCCGCCCGCGGACGTGAATTTCGAT
GACGCGGCGGCCATCCAGCAGGTGGTGAACGACTGGACCACCCTCTATGCCGCCACCGAGGAGAAGCACGACGCCGCCAC
CTTCGCGGCGATTCCCGCGGAGCGCCAGATGGCGGCGCGGGGTATCGAAGTCGGTCACATCTTCTATTTCGGCACCAAGT
ACAGCGCGCCCTTCAACGCCAAGGTGCTGGGGCCGGACGGCGCCGAGCATCTCATCCACATGGGCTCCTACGGCATCGGC
CCGTCCCGTCTCGTGGCCGCCATGATCGAGGCGTCCCATGACGACGCGGGAATCATCTGGCCCGACGCGGTCGCGCCGTT
CCAGGTGGGCATCCTCAATCTGAAGGTGGGCGACAGCGCCGTGGATGCGGCCTGCGCCGATCTTTACGCGAAACTAACCG
CTGCTGGCGTCGACGTACTGTACGACGACACGGACGAGCGGGCCGGCTCGAAGTTCGCGACCGCGGACCTGATCGGCCTG
CCCTGGCAGATCCTGGTGGGGCCGAAGAGCCTCGCCGACGGCAAGGTGGAACTGAAGCGGCGTGTGGACGGCTCGCGCGA
GCTGGTCACGCCCGCCGAGGCATTCGAGAGGCTCAAGGGATGA

Upstream 100 bases:

>100_bases
CAAGAGAGCGACCGGCCGTCTCGGGCTTGTGTTTTGCGGGGCCTTCCGCTTTCTGTCACCGGCACGTTTCTTTCAAACGC
CAAACCCTCAGAGTCTGCCG

Downstream 100 bases:

>100_bases
CGGGACGGCAGCGCATGGCCGCAGCCAAGGGTTCGCGGGCCGCCCCGCCGGACGCCGAACCCATGGGCACACGGCCATTC
GCTGCCTTCGAATGGATGCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRLSRYFLPILREVPKEADIVSHRLMLRAGMIRQESAGIYAWLPLGLRVLNKICNIIREEQNRTGAVELLMPTIQSADLW
RESGRYDDYGKEMLRIKDRHERDMLFGPTNEEMITEIVRGSIKSYKSLPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDA
YSFDLDAEGAKHSYNKMFVAYLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPPADVNFD
DAAAIQQVVNDWTTLYAATEEKHDAATFAAIPAERQMAARGIEVGHIFYFGTKYSAPFNAKVLGPDGAEHLIHMGSYGIG
PSRLVAAMIEASHDDAGIIWPDAVAPFQVGILNLKVGDSAVDAACADLYAKLTAAGVDVLYDDTDERAGSKFATADLIGL
PWQILVGPKSLADGKVELKRRVDGSRELVTPAEAFERLKG

Sequences:

>Translated_440_residues
MRLSRYFLPILREVPKEADIVSHRLMLRAGMIRQESAGIYAWLPLGLRVLNKICNIIREEQNRTGAVELLMPTIQSADLW
RESGRYDDYGKEMLRIKDRHERDMLFGPTNEEMITEIVRGSIKSYKSLPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDA
YSFDLDAEGAKHSYNKMFVAYLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPPADVNFD
DAAAIQQVVNDWTTLYAATEEKHDAATFAAIPAERQMAARGIEVGHIFYFGTKYSAPFNAKVLGPDGAEHLIHMGSYGIG
PSRLVAAMIEASHDDAGIIWPDAVAPFQVGILNLKVGDSAVDAACADLYAKLTAAGVDVLYDDTDERAGSKFATADLIGL
PWQILVGPKSLADGKVELKRRVDGSRELVTPAEAFERLKG
>Mature_440_residues
MRLSRYFLPILREVPKEADIVSHRLMLRAGMIRQESAGIYAWLPLGLRVLNKICNIIREEQNRTGAVELLMPTIQSADLW
RESGRYDDYGKEMLRIKDRHERDMLFGPTNEEMITEIVRGSIKSYKSLPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDA
YSFDLDAEGAKHSYNKMFVAYLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPPADVNFD
DAAAIQQVVNDWTTLYAATEEKHDAATFAAIPAERQMAARGIEVGHIFYFGTKYSAPFNAKVLGPDGAEHLIHMGSYGIG
PSRLVAAMIEASHDDAGIIWPDAVAPFQVGILNLKVGDSAVDAACADLYAKLTAAGVDVLYDDTDERAGSKFATADLIGL
PWQILVGPKSLADGKVELKRRVDGSRELVTPAEAFERLKG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=433, Percent_Identity=35.1039260969977, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=57.9185520361991, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI115532348, Length=414, Percent_Identity=32.6086956521739, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI193203271, Length=95, Percent_Identity=32.6315789473684, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=263, Percent_Identity=33.8403041825095, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656200, Length=428, Percent_Identity=30.3738317757009, Blast_Score=205, Evalue=6e-53,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_AZOC5 (A8I438)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001524602.1
- ProteinModelPortal:   A8I438
- SMR:   A8I438
- GeneID:   5688875
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1686
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- BioCyc:   ACAU438753:AZC_1686-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49024; Mature: 49024

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPILREVPKEADIVSHRLMLRAGMIRQESAGIYAWLPLGLRVLNKICNIIREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEEHHHHHHHHHHHHHHHHHH
QNRTGAVELLMPTIQSADLWRESGRYDDYGKEMLRIKDRHERDMLFGPTNEEMITEIVRG
CCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHH
SIKSYKSLPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDAYSFDLDAEGAKHSYNKMFVA
HHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
YLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPPADVNFD
HHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCC
DAAAIQQVVNDWTTLYAATEEKHDAATFAAIPAERQMAARGIEVGHIFYFGTKYSAPFNA
HHHHHHHHHHHHHHEEEECCCCCCCHHEEECCCHHHHHHCCCEEEEEEEEECEECCCCCE
KVLGPDGAEHLIHMGSYGIGPSRLVAAMIEASHDDAGIIWPDAVAPFQVGILNLKVGDSA
EEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEEEECCHH
VDAACADLYAKLTAAGVDVLYDDTDERAGSKFATADLIGLPWQILVGPKSLADGKVELKR
HHHHHHHHHHHHHHCCCEEEECCCCHHCCCCCCHHHHHCCCEEEEECCCHHCCCHHHHHH
RVDGSRELVTPAEAFERLKG
HCCCCCCEECHHHHHHHHCC
>Mature Secondary Structure
MRLSRYFLPILREVPKEADIVSHRLMLRAGMIRQESAGIYAWLPLGLRVLNKICNIIREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCEEEEHHHHHHHHHHHHHHHHHH
QNRTGAVELLMPTIQSADLWRESGRYDDYGKEMLRIKDRHERDMLFGPTNEEMITEIVRG
CCCCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHH
SIKSYKSLPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDAYSFDLDAEGAKHSYNKMFVA
HHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
YLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPPADVNFD
HHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCC
DAAAIQQVVNDWTTLYAATEEKHDAATFAAIPAERQMAARGIEVGHIFYFGTKYSAPFNA
HHHHHHHHHHHHHHEEEECCCCCCCHHEEECCCHHHHHHCCCEEEEEEEEECEECCCCCE
KVLGPDGAEHLIHMGSYGIGPSRLVAAMIEASHDDAGIIWPDAVAPFQVGILNLKVGDSA
EEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEEEECCHH
VDAACADLYAKLTAAGVDVLYDDTDERAGSKFATADLIGLPWQILVGPKSLADGKVELKR
HHHHHHHHHHHHHHCCCEEEECCCCHHCCCCCCHHHHHCCCEEEEECCCHHCCCHHHHHH
RVDGSRELVTPAEAFERLKG
HCCCCCCEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA