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The map label for this gene is nuoE1 [H]

Identifier: 158423295

GI number: 158423295

Start: 1912209

End: 1912883

Strand: Direct

Name: nuoE1 [H]

Synonym: AZC_1671

Alternate gene names: 158423295

Gene position: 1912209-1912883 (Clockwise)

Preceding gene: 158423294

Following gene: 158423296

Centisome position: 35.61

GC content: 69.33

Gene sequence:

>675_bases
ATGTCCGTCCGTCGTCTCGCTGCCGAGCAGCCCGCCAGCTTCGCCTTCTCTCCGGAGAACGAGGCCTGGGCCGATCGCCA
GATCGCCAAGTATCCCGAAGGCCGGCAGGCCAGCGCCGTGATCCCGCTCCTGTGGAAGGCGCAGGAGCAGTTCGGCGGCT
GGCTCCCCGAGCCCGCGATCCGGGTCGTCGCCGACAAGCTCGGCATGGCCTATATCCGCGTGCTCGAGATCGCCACCTTC
TACACGATGTTCAACCTTGAGCCCGTGGGCCGCCACTTCGTGCAGCTCTGCGGCACGACGCCCTGCGCCCTGCGCGGCGC
CGACAAGCTCAAGGAAGTGTGCCATCGCAAGATCGGCCCCGAGCGGCACGTGACGGAAGACGGTGCGCTGTCCTGGCTCG
AGGTGGAATGCCTCGGCGCCTGCGCCAACGCGCCGATGGTCCAGGTGAACTACGACTATTTCGAGGACCTCACCCCCGAG
AGCCTCGAGAAGCTGCTGGATGATCTCCAGGCAGGGCGCCCGGTGAAGACCGGCTCCCAGACCGGCCGTCAGGCCTCGGC
GCCCGAGGGTGGCCGCACGTCCCTGACGGATCCCTCGCTCTACAACCGCAAGCCGGCCGAGCCGGCGGCGGCCGCCGACG
CGCCGAAGACCGGCGCCGGCGATGCGGAGGCTTGA

Upstream 100 bases:

>100_bases
CGCAGGTGCTGAAGCTCTACAAGAACGGCGTCACCGTCTATTGCGCGATCGCGCAGGCGGGCGGTGCCTATAACAACTGT
CACGTAGCCGGAACGCGCTG

Downstream 100 bases:

>100_bases
GCCATGCTTCAGGACAAGGACCGCATCTTCACGAACATCTACGGCTACGAGGACAGTGGCCTCAAGGGTGCTCTGAAGCG
CGGCAACTGGGACGGCACCA

Product: NADH dehydrogenase

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 1; NDH-1 subunit E 1 [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSVRRLAAEQPASFAFSPENEAWADRQIAKYPEGRQASAVIPLLWKAQEQFGGWLPEPAIRVVADKLGMAYIRVLEIATF
YTMFNLEPVGRHFVQLCGTTPCALRGADKLKEVCHRKIGPERHVTEDGALSWLEVECLGACANAPMVQVNYDYFEDLTPE
SLEKLLDDLQAGRPVKTGSQTGRQASAPEGGRTSLTDPSLYNRKPAEPAAAADAPKTGAGDAEA

Sequences:

>Translated_224_residues
MSVRRLAAEQPASFAFSPENEAWADRQIAKYPEGRQASAVIPLLWKAQEQFGGWLPEPAIRVVADKLGMAYIRVLEIATF
YTMFNLEPVGRHFVQLCGTTPCALRGADKLKEVCHRKIGPERHVTEDGALSWLEVECLGACANAPMVQVNYDYFEDLTPE
SLEKLLDDLQAGRPVKTGSQTGRQASAPEGGRTSLTDPSLYNRKPAEPAAAADAPKTGAGDAEA
>Mature_223_residues
SVRRLAAEQPASFAFSPENEAWADRQIAKYPEGRQASAVIPLLWKAQEQFGGWLPEPAIRVVADKLGMAYIRVLEIATFY
TMFNLEPVGRHFVQLCGTTPCALRGADKLKEVCHRKIGPERHVTEDGALSWLEVECLGACANAPMVQVNYDYFEDLTPES
LEKLLDDLQAGRPVKTGSQTGRQASAPEGGRTSLTDPSLYNRKPAEPAAAADAPKTGAGDAEA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=186, Percent_Identity=47.8494623655914, Blast_Score=183, Evalue=1e-46,
Organism=Escherichia coli, GI1788621, Length=159, Percent_Identity=31.4465408805031, Blast_Score=84, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17561328, Length=187, Percent_Identity=49.7326203208556, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI18859877, Length=185, Percent_Identity=45.945945945946, Blast_Score=170, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21355353, Length=184, Percent_Identity=39.1304347826087, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24373; Mature: 24242

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRLAAEQPASFAFSPENEAWADRQIAKYPEGRQASAVIPLLWKAQEQFGGWLPEPAI
CCHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
RVVADKLGMAYIRVLEIATFYTMFNLEPVGRHFVQLCGTTPCALRGADKLKEVCHRKIGP
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCC
ERHVTEDGALSWLEVECLGACANAPMVQVNYDYFEDLTPESLEKLLDDLQAGRPVKTGSQ
CCCCCCCCCCEEHHHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
TGRQASAPEGGRTSLTDPSLYNRKPAEPAAAADAPKTGAGDAEA
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVRRLAAEQPASFAFSPENEAWADRQIAKYPEGRQASAVIPLLWKAQEQFGGWLPEPAI
CHHHHHHCCCCCEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
RVVADKLGMAYIRVLEIATFYTMFNLEPVGRHFVQLCGTTPCALRGADKLKEVCHRKIGP
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCC
ERHVTEDGALSWLEVECLGACANAPMVQVNYDYFEDLTPESLEKLLDDLQAGRPVKTGSQ
CCCCCCCCCCEEHHHHHHHHHCCCCEEEEEHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
TGRQASAPEGGRTSLTDPSLYNRKPAEPAAAADAPKTGAGDAEA
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]