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The map label for this gene is mdlB [H]

Identifier: 158423279

GI number: 158423279

Start: 1894015

End: 1895163

Strand: Reverse

Name: mdlB [H]

Synonym: AZC_1655

Alternate gene names: 158423279

Gene position: 1895163-1894015 (Counterclockwise)

Preceding gene: 158423280

Following gene: 158423259

Centisome position: 35.29

GC content: 69.97

Gene sequence:

>1149_bases
ATGGTGACCCTGCTGAATGTCGAGGACGCACGTCGCCTCGCCCGCCGCCGCCTGCCCCGCGGCCTGTTCGAATATCTCGA
TCGCGGCACGGAGGATGAAGTCTCCATCGCCGGCAACCGGGCCGGGCTCGATGCCATCCGCCTCGCGCCCTTCGCGCTGG
AGGACGTGTCGCAGCGCGCGCAGGACACCGTGCTGTTCGGCACGCCGCAACCCTGCCCGCTGGTGATCGCGCCGACCGCC
GTCGCCGGCCTGATGAGCTATGACGGCGAGGTGGCCATGGCCCGCGCCGCCAAGGCGCATGACATTCCCTTCTGCGTCTC
CACCCAGTCCATGACGTCGATCGAGACCATCGCGCGGGACAGCGGTGCCCGCCTCTGGTTCCAGCTCTATGTGTGGAAGA
ACCGGGCGCGCACCTTCGCCTTGCTCGACCGGGCGGCGGGGGCCGGCGCGGATACGCTGGTGCTCACCGTGGACACCGCC
GTCTCGCCCAAGCGTGAGTACAACCAGCGCAATGGCTTCGGCATCCCGCTCAAGCCCTCGGTGCGGGCGGGCATCGACGT
GCTCTGTCATCCGCGCTGGTTCGCGGACGTCTTCCTGCGCACGCTGCGCACGACCGGCATGCCCACCTACGCGCACTATC
CGGATGAATTCCGGACCGCGCTCGGCCGCGCGGTGGTTGGGGACGAGATCAGCCTTGCCACCGACGTCTCATGGAAGGAT
GTGGCGGCGCTGCGCGCGCACTGGAAGGGGCGGCTGATCCTGAAGGGCATCCTGCGGGCCTCGGACGCCACCCGCGCCAT
CGCCCACGGGGTGGACGGCATCGTGGTCTCCAACCACGGCGCCCGCAATCTCGATTGCGCGCCGCATCCGGCGCATGTCC
TGCCGGCCATCGTCGCAGCGGCGGGCGGACGGCTGACTGTACTCGCAGACAGCGGCGTGCGGCGCGGCAGCGACATCGCC
AAATATCTGGCGCTGGGGGCCGATGGCGTTCTCGTGGGCCGCGCCCCGCTCTACGGCCTTGCCGCCGCCGGTACACCGGG
TGCGTCGCGAGTCATCGAACTGCTGCGGGCAGAACTCGACACCACCATGGCCCTGCTCGGCGTCACCCGGCTCGACCAAC
TCCCCCGCACGCTCCAGATGGAGGCGTGA

Upstream 100 bases:

>100_bases
GTCAAGCCGCAGGAGGCGACGCTGGAAGAGATGGAATCGCTCTGGCAGCAGGCCAAGCAGGCCGAACGGGCCCCCGCGAC
CACGACTGCCGCCTCCTGAC

Downstream 100 bases:

>100_bases
GGGACGGGACCTCCCATCGGCATGGCGTTCGCCCAATCTCACCTAGGGGAAGCTGAACTCCCGCTCAAGGCTGACGCCGA
AGGTCATCTGGTTCACGCCG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MVTLLNVEDARRLARRRLPRGLFEYLDRGTEDEVSIAGNRAGLDAIRLAPFALEDVSQRAQDTVLFGTPQPCPLVIAPTA
VAGLMSYDGEVAMARAAKAHDIPFCVSTQSMTSIETIARDSGARLWFQLYVWKNRARTFALLDRAAGAGADTLVLTVDTA
VSPKREYNQRNGFGIPLKPSVRAGIDVLCHPRWFADVFLRTLRTTGMPTYAHYPDEFRTALGRAVVGDEISLATDVSWKD
VAALRAHWKGRLILKGILRASDATRAIAHGVDGIVVSNHGARNLDCAPHPAHVLPAIVAAAGGRLTVLADSGVRRGSDIA
KYLALGADGVLVGRAPLYGLAAAGTPGASRVIELLRAELDTTMALLGVTRLDQLPRTLQMEA

Sequences:

>Translated_382_residues
MVTLLNVEDARRLARRRLPRGLFEYLDRGTEDEVSIAGNRAGLDAIRLAPFALEDVSQRAQDTVLFGTPQPCPLVIAPTA
VAGLMSYDGEVAMARAAKAHDIPFCVSTQSMTSIETIARDSGARLWFQLYVWKNRARTFALLDRAAGAGADTLVLTVDTA
VSPKREYNQRNGFGIPLKPSVRAGIDVLCHPRWFADVFLRTLRTTGMPTYAHYPDEFRTALGRAVVGDEISLATDVSWKD
VAALRAHWKGRLILKGILRASDATRAIAHGVDGIVVSNHGARNLDCAPHPAHVLPAIVAAAGGRLTVLADSGVRRGSDIA
KYLALGADGVLVGRAPLYGLAAAGTPGASRVIELLRAELDTTMALLGVTRLDQLPRTLQMEA
>Mature_382_residues
MVTLLNVEDARRLARRRLPRGLFEYLDRGTEDEVSIAGNRAGLDAIRLAPFALEDVSQRAQDTVLFGTPQPCPLVIAPTA
VAGLMSYDGEVAMARAAKAHDIPFCVSTQSMTSIETIARDSGARLWFQLYVWKNRARTFALLDRAAGAGADTLVLTVDTA
VSPKREYNQRNGFGIPLKPSVRAGIDVLCHPRWFADVFLRTLRTTGMPTYAHYPDEFRTALGRAVVGDEISLATDVSWKD
VAALRAHWKGRLILKGILRASDATRAIAHGVDGIVVSNHGARNLDCAPHPAHVLPAIVAAAGGRLTVLADSGVRRGSDIA
KYLALGADGVLVGRAPLYGLAAAGTPGASRVIELLRAELDTTMALLGVTRLDQLPRTLQMEA

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=384, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=9e-51,
Organism=Homo sapiens, GI54234014, Length=379, Percent_Identity=32.9815303430079, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI7705393, Length=379, Percent_Identity=32.9815303430079, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1790033, Length=369, Percent_Identity=37.3983739837398, Blast_Score=227, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI193208036, Length=384, Percent_Identity=33.8541666666667, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI32566217, Length=296, Percent_Identity=31.7567567567568, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6323587, Length=383, Percent_Identity=30.0261096605744, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281363140, Length=380, Percent_Identity=34.7368421052632, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI78707190, Length=380, Percent_Identity=34.7368421052632, Blast_Score=197, Evalue=8e-51,
Organism=Drosophila melanogaster, GI78707188, Length=380, Percent_Identity=34.7368421052632, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 41073; Mature: 41073

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLLNVEDARRLARRRLPRGLFEYLDRGTEDEVSIAGNRAGLDAIRLAPFALEDVSQRA
CEEEECHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCHHHHCCHHHHHHHHHHH
QDTVLFGTPQPCPLVIAPTAVAGLMSYDGEVAMARAAKAHDIPFCVSTQSMTSIETIARD
HCEEEECCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHCC
SGARLWFQLYVWKNRARTFALLDRAAGAGADTLVLTVDTAVSPKREYNQRNGFGIPLKPS
CCCEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCC
VRAGIDVLCHPRWFADVFLRTLRTTGMPTYAHYPDEFRTALGRAVVGDEISLATDVSWKD
HHCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHH
VAALRAHWKGRLILKGILRASDATRAIAHGVDGIVVSNHGARNLDCAPHPAHVLPAIVAA
HHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
AGGRLTVLADSGVRRGSDIAKYLALGADGVLVGRAPLYGLAAAGTPGASRVIELLRAELD
CCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHH
TTMALLGVTRLDQLPRTLQMEA
HHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MVTLLNVEDARRLARRRLPRGLFEYLDRGTEDEVSIAGNRAGLDAIRLAPFALEDVSQRA
CEEEECHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCHHHHCCHHHHHHHHHHH
QDTVLFGTPQPCPLVIAPTAVAGLMSYDGEVAMARAAKAHDIPFCVSTQSMTSIETIARD
HCEEEECCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHCC
SGARLWFQLYVWKNRARTFALLDRAAGAGADTLVLTVDTAVSPKREYNQRNGFGIPLKPS
CCCEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCCCCCC
VRAGIDVLCHPRWFADVFLRTLRTTGMPTYAHYPDEFRTALGRAVVGDEISLATDVSWKD
HHCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHH
VAALRAHWKGRLILKGILRASDATRAIAHGVDGIVVSNHGARNLDCAPHPAHVLPAIVAA
HHHHHHHCCCHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
AGGRLTVLADSGVRRGSDIAKYLALGADGVLVGRAPLYGLAAAGTPGASRVIELLRAELD
CCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHH
TTMALLGVTRLDQLPRTLQMEA
HHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]