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The map label for this gene is narI [H]

Identifier: 158423052

GI number: 158423052

Start: 1629390

End: 1630169

Strand: Direct

Name: narI [H]

Synonym: AZC_1428

Alternate gene names: 158423052

Gene position: 1629390-1630169 (Clockwise)

Preceding gene: 158423051

Following gene: 158423053

Centisome position: 30.34

GC content: 66.54

Gene sequence:

>780_bases
ATGTCCGCGCTCAACTCGGCCCTGTTCGGCTGGTACCCCTATCTCTGTCTCACCGTCTTCCTGTTCGGCAGCCTCATCCG
CTTCGATCGGGAGCAATACACGTGGAAGACCGGCTCCTCGCAGCTCCTGCGCCGCCGCCAGCTGCGCCTCGGCTCGAACC
TCTTCCACATCGGCATCCTCGCCATCTTCGCCGGCCACACGGTGGGTCTGCTGACGCCCATCTGGGTGTTCGATGCGATG
GGCGTCAGCCATTCCTTCAAGCAGGGACTGGCCATCACGGCCGGCGGCATCGCCGGTGTGCTGTGCTTCATCGGCATCTC
GCTCCTGGCGCACCGCCGGCTGTTCGATCCGCGCATCCGCGCCACGTCGAGCTTCGGCGATACGGCCATCCTGCTCATCC
TGTGGGTGCAGCTCACCCTCGGCCTCTTCACCATCTTCGTCTCGCTCGGCCATATGGACGGGCATGAGATGGTGAAGTTC
ATGAACTGGGCGCAGGGCATCCTCACCCTCCAGCCGCAGGCGGCGGCCTATGTGGCGGACGTGAACCCCATCTTCAAGGC
GCACATGCTGCTGGGGCTCACCATCTTCCTCATCTTCCCCTTCACCCGCCTCGTGCATGTGTGGAGCGCGCCGGTCTGGT
ATCTGGGCCGCCCCGGCTATCAGGTGGTGCGCACGCGCTTCGCCCATGGCTCCGGCCAGCCGCGCCGCACGGCATCCGCG
CCGGTCGTCGCTCCGGCGCCCCGTGCCACGTCCATTCCCGCACAGCGTCCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CGGGGAGGCGCTCGATGGCTGTTCCGTGGATCGCCTGCGCACCCAGATCCGCGCCGCCCAGCGCGACGCGCGCCTTGCCA
CCGCCTGAGGGAGACGATCC

Downstream 100 bases:

>100_bases
AGGCCATGGTTACGCTCGTCATCGACCATGCCGCCACCCGCGCCGATCATTCCCACGACCACCCCCATGAGCACGCGCCG
CTGGCACGGGACACGGCGCC

Product: nitrate reductase subunit gamma protein

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSALNSALFGWYPYLCLTVFLFGSLIRFDREQYTWKTGSSQLLRRRQLRLGSNLFHIGILAIFAGHTVGLLTPIWVFDAM
GVSHSFKQGLAITAGGIAGVLCFIGISLLAHRRLFDPRIRATSSFGDTAILLILWVQLTLGLFTIFVSLGHMDGHEMVKF
MNWAQGILTLQPQAAAYVADVNPIFKAHMLLGLTIFLIFPFTRLVHVWSAPVWYLGRPGYQVVRTRFAHGSGQPRRTASA
PVVAPAPRATSIPAQRPAE

Sequences:

>Translated_259_residues
MSALNSALFGWYPYLCLTVFLFGSLIRFDREQYTWKTGSSQLLRRRQLRLGSNLFHIGILAIFAGHTVGLLTPIWVFDAM
GVSHSFKQGLAITAGGIAGVLCFIGISLLAHRRLFDPRIRATSSFGDTAILLILWVQLTLGLFTIFVSLGHMDGHEMVKF
MNWAQGILTLQPQAAAYVADVNPIFKAHMLLGLTIFLIFPFTRLVHVWSAPVWYLGRPGYQVVRTRFAHGSGQPRRTASA
PVVAPAPRATSIPAQRPAE
>Mature_258_residues
SALNSALFGWYPYLCLTVFLFGSLIRFDREQYTWKTGSSQLLRRRQLRLGSNLFHIGILAIFAGHTVGLLTPIWVFDAMG
VSHSFKQGLAITAGGIAGVLCFIGISLLAHRRLFDPRIRATSSFGDTAILLILWVQLTLGLFTIFVSLGHMDGHEMVKFM
NWAQGILTLQPQAAAYVADVNPIFKAHMLLGLTIFLIFPFTRLVHVWSAPVWYLGRPGYQVVRTRFAHGSGQPRRTASAP
VVAPAPRATSIPAQRPAE

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=226, Percent_Identity=51.3274336283186, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1787738, Length=223, Percent_Identity=49.7757847533632, Blast_Score=200, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 28693; Mature: 28562

Theoretical pI: Translated: 11.35; Mature: 11.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALNSALFGWYPYLCLTVFLFGSLIRFDREQYTWKTGSSQLLRRRQLRLGSNLFHIGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCHHHHHHH
AIFAGHTVGLLTPIWVFDAMGVSHSFKQGLAITAGGIAGVLCFIGISLLAHRRLFDPRIR
HHHHCCHHHHHHHHHHHHHHCCCHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHCCCHHH
ATSSFGDTAILLILWVQLTLGLFTIFVSLGHMDGHEMVKFMNWAQGILTLQPQAAAYVAD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCHHHHHHC
VNPIFKAHMLLGLTIFLIFPFTRLVHVWSAPVWYLGRPGYQVVRTRFAHGSGQPRRTASA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCC
PVVAPAPRATSIPAQRPAE
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SALNSALFGWYPYLCLTVFLFGSLIRFDREQYTWKTGSSQLLRRRQLRLGSNLFHIGIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHCCCHHHHHHH
AIFAGHTVGLLTPIWVFDAMGVSHSFKQGLAITAGGIAGVLCFIGISLLAHRRLFDPRIR
HHHHCCHHHHHHHHHHHHHHCCCHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHCCCHHH
ATSSFGDTAILLILWVQLTLGLFTIFVSLGHMDGHEMVKFMNWAQGILTLQPQAAAYVAD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCHHHHHHC
VNPIFKAHMLLGLTIFLIFPFTRLVHVWSAPVWYLGRPGYQVVRTRFAHGSGQPRRTASA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCC
PVVAPAPRATSIPAQRPAE
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]