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The map label for this gene is nodW [H]

Identifier: 158422986

GI number: 158422986

Start: 1536521

End: 1536979

Strand: Direct

Name: nodW [H]

Synonym: AZC_1362

Alternate gene names: 158422986

Gene position: 1536521-1536979 (Clockwise)

Preceding gene: 158422985

Following gene: 158422989

Centisome position: 28.61

GC content: 65.36

Gene sequence:

>459_bases
TTGTGGACGGGCCCGCCTATGTTGGGGACAGGACGGCGCTGGCGCCGTCAGTGCCATAGAGGTGTGTTTCGCGTGTCCCA
CGTTCCGATGATTTCGATCATCGACGATGACGAGTCCGTGCGTCTCGCCACAGAAAGTCTCGTCCGCTCCCTTGGACTGG
GCGCCAGCGCTTTCGCCTCCGCCGAAGCCTTCCTGAGCTCGCCGGAGCGGCAGAGTACCGCGTGTGTCATCACCGATGTT
CAGATGCCCGGCATGAGCGGCATCGATCTGCAGGCGCGCCTGCGCTCCGATGGCGATGGCGTCCCGCTCATCTTCATCAC
TGCCTTTCCCGAGGAGAGGATCCGCAGCCAGGCCCACGCCGGCGGCGCAATCGGCTTCCTGGCAAAGCCCTTCGAGGGCG
ACGAGATGATCCGGTGCATCGACGATGCCCTGCGGACGACGGGGCAGGCGCGCCCTTGA

Upstream 100 bases:

>100_bases
AAATGGAAGCCCAGTCCCTGGCCGATCTCGTACGCATGGCGGAGGCGCTGGGCATCCGCGCGGCGAAAGGATAGCGGAGC
AAACAAGTGTATGGTTCAGA

Downstream 100 bases:

>100_bases
GCCGGGCGCGGCGCGGACGGCTGACGCGGGTCATGTCCCTCCTGTCGACGGCGCATGTGAAATCACGCGACGGACTATCG
ATCGACGGAGGTTCAGATCG

Product: two-component response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MWTGPPMLGTGRRWRRQCHRGVFRVSHVPMISIIDDDESVRLATESLVRSLGLGASAFASAEAFLSSPERQSTACVITDV
QMPGMSGIDLQARLRSDGDGVPLIFITAFPEERIRSQAHAGGAIGFLAKPFEGDEMIRCIDDALRTTGQARP

Sequences:

>Translated_152_residues
MWTGPPMLGTGRRWRRQCHRGVFRVSHVPMISIIDDDESVRLATESLVRSLGLGASAFASAEAFLSSPERQSTACVITDV
QMPGMSGIDLQARLRSDGDGVPLIFITAFPEERIRSQAHAGGAIGFLAKPFEGDEMIRCIDDALRTTGQARP
>Mature_152_residues
MWTGPPMLGTGRRWRRQCHRGVFRVSHVPMISIIDDDESVRLATESLVRSLGLGASAFASAEAFLSSPERQSTACVITDV
QMPGMSGIDLQARLRSDGDGVPLIFITAFPEERIRSQAHAGGAIGFLAKPFEGDEMIRCIDDALRTTGQARP

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=26.9565217391304, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 16528; Mature: 16528

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTGPPMLGTGRRWRRQCHRGVFRVSHVPMISIIDDDESVRLATESLVRSLGLGASAFAS
CCCCCCCCCCCHHHHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHH
AEAFLSSPERQSTACVITDVQMPGMSGIDLQARLRSDGDGVPLIFITAFPEERIRSQAHA
HHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHCC
GGAIGFLAKPFEGDEMIRCIDDALRTTGQARP
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MWTGPPMLGTGRRWRRQCHRGVFRVSHVPMISIIDDDESVRLATESLVRSLGLGASAFAS
CCCCCCCCCCCHHHHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHH
AEAFLSSPERQSTACVITDVQMPGMSGIDLQARLRSDGDGVPLIFITAFPEERIRSQAHA
HHHHHCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCHHHHHHHHCC
GGAIGFLAKPFEGDEMIRCIDDALRTTGQARP
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]