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The map label for this gene is nodW [H]

Identifier: 158422985

GI number: 158422985

Start: 1535838

End: 1536494

Strand: Direct

Name: nodW [H]

Synonym: AZC_1361

Alternate gene names: 158422985

Gene position: 1535838-1536494 (Clockwise)

Preceding gene: 158422984

Following gene: 158422986

Centisome position: 28.6

GC content: 65.75

Gene sequence:

>657_bases
GTGATCAGCCGTACCCAAGGCCGAAGCCCGACCCCTACTCAGGGACCCGTCGTCATCGTCGTGGACGACGACGCAGACCT
GCGCGATGCCTTGACCAGCCTGTTCCGCTCCATCGGCCTGGAGGTGGTGTCGTTCGGATCGGCTCCGGAATTGCTCGCCG
CCAAGCTGCCGGACACGCCCCGCTGCCTCGTTCTGGACATCCGCCTGCCCGGCGTCAGCGGCCTTGATTTCCAGAGCCAG
CTTCAGAGGTCCGATCTGGACCTGCCCATCATCTTCATGACCGGGCATGGCGATATCCCCATGACGGTGCGGGCGATGAA
GGCGGGCGCGGTGGATTTCCTCACCAAGCCCTTCCGGGACCAGGACATGCTGGATGCGGTTTCGGCCGCCATCGAGATCG
ACCGCACCCGGCGCGCCGAGCGGGAAGCCGCATCGCACCTGCGCGCCCTTTACGCCACGCTGACCGAGCGTGAGAGGCAG
ATCATGGCGCTCGTGACGTCCGGCCTCATGAACAAGCAGGTGGCGGGCGAGGTGGGCCTCAGCGAGATCACCGTGAAGAT
TCACCGCGGCAACGTCATGCGGAAAATGGAAGCCCAGTCCCTGGCCGATCTCGTACGCATGGCGGAGGCGCTGGGCATCC
GCGCGGCGAAAGGATAG

Upstream 100 bases:

>100_bases
GGATTCTGGCCGAGCCTGCGCCGCAAGGCGGCCTGCGCCTGACCTTCACCCTCCCGCTCGCCGGCGATCTTCCGCCGGCG
CCCCTCAAGGAACCTCTCTC

Downstream 100 bases:

>100_bases
CGGAGCAAACAAGTGTATGGTTCAGATTGTGGACGGGCCCGCCTATGTTGGGGACAGGACGGCGCTGGCGCCGTCAGTGC
CATAGAGGTGTGTTTCGCGT

Product: two-component response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MISRTQGRSPTPTQGPVVIVVDDDADLRDALTSLFRSIGLEVVSFGSAPELLAAKLPDTPRCLVLDIRLPGVSGLDFQSQ
LQRSDLDLPIIFMTGHGDIPMTVRAMKAGAVDFLTKPFRDQDMLDAVSAAIEIDRTRRAEREAASHLRALYATLTERERQ
IMALVTSGLMNKQVAGEVGLSEITVKIHRGNVMRKMEAQSLADLVRMAEALGIRAAKG

Sequences:

>Translated_218_residues
MISRTQGRSPTPTQGPVVIVVDDDADLRDALTSLFRSIGLEVVSFGSAPELLAAKLPDTPRCLVLDIRLPGVSGLDFQSQ
LQRSDLDLPIIFMTGHGDIPMTVRAMKAGAVDFLTKPFRDQDMLDAVSAAIEIDRTRRAEREAASHLRALYATLTERERQ
IMALVTSGLMNKQVAGEVGLSEITVKIHRGNVMRKMEAQSLADLVRMAEALGIRAAKG
>Mature_218_residues
MISRTQGRSPTPTQGPVVIVVDDDADLRDALTSLFRSIGLEVVSFGSAPELLAAKLPDTPRCLVLDIRLPGVSGLDFQSQ
LQRSDLDLPIIFMTGHGDIPMTVRAMKAGAVDFLTKPFRDQDMLDAVSAAIEIDRTRRAEREAASHLRALYATLTERERQ
IMALVTSGLMNKQVAGEVGLSEITVKIHRGNVMRKMEAQSLADLVRMAEALGIRAAKG

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=38.2608695652174, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788222, Length=202, Percent_Identity=28.2178217821782, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1790102, Length=194, Percent_Identity=29.8969072164948, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786747, Length=158, Percent_Identity=24.6835443037975, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=30.2521008403361, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788521, Length=202, Percent_Identity=30.6930693069307, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23722; Mature: 23722

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRTQGRSPTPTQGPVVIVVDDDADLRDALTSLFRSIGLEVVSFGSAPELLAAKLPDTP
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHEEECCCCHHHHHHCCCCCC
RCLVLDIRLPGVSGLDFQSQLQRSDLDLPIIFMTGHGDIPMTVRAMKAGAVDFLTKPFRD
CEEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHCCCCC
QDMLDAVSAAIEIDRTRRAEREAASHLRALYATLTERERQIMALVTSGLMNKQVAGEVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SEITVKIHRGNVMRKMEAQSLADLVRMAEALGIRAAKG
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MISRTQGRSPTPTQGPVVIVVDDDADLRDALTSLFRSIGLEVVSFGSAPELLAAKLPDTP
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHEEECCCCHHHHHHCCCCCC
RCLVLDIRLPGVSGLDFQSQLQRSDLDLPIIFMTGHGDIPMTVRAMKAGAVDFLTKPFRD
CEEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHHCCCCC
QDMLDAVSAAIEIDRTRRAEREAASHLRALYATLTERERQIMALVTSGLMNKQVAGEVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SEITVKIHRGNVMRKMEAQSLADLVRMAEALGIRAAKG
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]