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The map label for this gene is dctA [H]

Identifier: 158422949

GI number: 158422949

Start: 1483057

End: 1484430

Strand: Reverse

Name: dctA [H]

Synonym: AZC_1325

Alternate gene names: 158422949

Gene position: 1484430-1483057 (Counterclockwise)

Preceding gene: 158422950

Following gene: 158422947

Centisome position: 27.64

GC content: 65.65

Gene sequence:

>1374_bases
ATGCGGCCCAATAGCAAAGCCGCCGGAAGCAGCGGCGGCAGCACGGGAGTGAAGTTCGTTATGGCCCGCTTACGCCAGCT
CTATGTTCAGGTGATCATCGCCATCCTGCTCGGCATCGCCCTGGGGATTCTGGCGCCGGCGACCGCCGTCGAGATGAAGC
CGCTCGGCGATGCCTTTATCGGGCTGCTGCGGATGATGCTGGGGCCGATCATCTTCTGCTCCGTGGTTCTGGGGCTCACC
CACGTGCGGGATATGGGCCAGCTCGGCCGCCTCGCCTTCAAGGCGCTGCTCTATTTCGAGATCCTCACCACCGTCGGCAT
GGCGCTCGGATTCGTGGTGGTGAACGTGTTCGAGCCCGGCGCGGGCCTCCACGCCACCAGCCTCGCCGTCAACGAGAGCG
TAGCCAGGATCTCGAACACCGCCAGCCACTTCACCGCCGTCGGCTTCTTCCTTTCCATCATTCCCAACACGCTGGTGGAC
GCGTTCGCCAAGGGCGAGATCCTGCAGGTGCTCTTCATCTCCGTGCTCACCGGCGCGGCTCTCAGCATCGGCGGCAAGAA
GGATTCGCCCATCCTGCGCGGCATCGAGGAAGGGCAGGATGTCCTGTTCCGCATCCTCGGCTTCATCATGCGCGTGGCCC
CCATCGGTGCGTTTGGCGCCATCGCGGCGGCCGTCGGCGCTCACGGTTCGGGCACGCTGATCTATCTCGCCAAGCTCGTC
GGCCTTTACTACGTCACGTCCCTCGCCTTCGTCTTCCTGGTCCTTGGCTCAGTAGCTGGCATGGCCGGGCTGTCCATCCT
GAAGCTCATCCGCCTGGTGCGCGAGGAACTGCTGCTGGTGCTCGGCACCGCGTCTGGCGAGGTGGCCCTGCCCCGGCTGA
TGCAGAAGCTGGAAAAGGCCGGCTGCGACGAGGCGGTGGTGGGGTTTGTACTGCCCGCCGGCTACAGCTTCAATCTGGAC
GGCACCGCCATCTACATGGCCATCGCGGTGGGCTTCATCGCCCAGGCCACCGATACACCCTTTTCGCTTGGCCAGCAGAT
CGGCGTGCTCGCCATCATGCTGCTCACCTCAAAGGGCGGCACCACCGTCGCCGGAGGCGCCTTCATCAAGCTCGCCGCCA
CGTTGCAGACGGTGCGTGCGCTGCCGCTCAACGGGCTGGGCCTTCTGTTTGGCGTGGACCGGCTGATGGCCACCTGCACC
GCACTCACCAATGTGGTGGGGAACACCGTTGCCGTGTTTGTCATCGCCCGGTGGGAGAATGCGTTCAACCGGACCAAGTT
CGACAGCTACATGGCCGAGCTCGCCGCGCCGGCCGGTGCCGGCCCGCAGGCGTCGGCCGTGGCGGTCCCCGCATCCGCCA
AGGCCGGTGAGTGA

Upstream 100 bases:

>100_bases
ATTACACCGCCCGGTCGGCACGCTGGGATGAACCATACCGAAACGGCATGGATATGTATCCATCGACGGACTTGGACATA
GCCCGTTCCTGACGTTTGTA

Downstream 100 bases:

>100_bases
AACCCGCATGTCAGCGGACATAGTCATCTACCACCTTGGCGCCGTACCACGCGCCTGCAGCCACACGCTCCACGATGACG
CCGCGCACCACATCGAAGAG

Product: C4-dicarboxylate transporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRPNSKAAGSSGGSTGVKFVMARLRQLYVQVIIAILLGIALGILAPATAVEMKPLGDAFIGLLRMMLGPIIFCSVVLGLT
HVRDMGQLGRLAFKALLYFEILTTVGMALGFVVVNVFEPGAGLHATSLAVNESVARISNTASHFTAVGFFLSIIPNTLVD
AFAKGEILQVLFISVLTGAALSIGGKKDSPILRGIEEGQDVLFRILGFIMRVAPIGAFGAIAAAVGAHGSGTLIYLAKLV
GLYYVTSLAFVFLVLGSVAGMAGLSILKLIRLVREELLLVLGTASGEVALPRLMQKLEKAGCDEAVVGFVLPAGYSFNLD
GTAIYMAIAVGFIAQATDTPFSLGQQIGVLAIMLLTSKGGTTVAGGAFIKLAATLQTVRALPLNGLGLLFGVDRLMATCT
ALTNVVGNTVAVFVIARWENAFNRTKFDSYMAELAAPAGAGPQASAVAVPASAKAGE

Sequences:

>Translated_457_residues
MRPNSKAAGSSGGSTGVKFVMARLRQLYVQVIIAILLGIALGILAPATAVEMKPLGDAFIGLLRMMLGPIIFCSVVLGLT
HVRDMGQLGRLAFKALLYFEILTTVGMALGFVVVNVFEPGAGLHATSLAVNESVARISNTASHFTAVGFFLSIIPNTLVD
AFAKGEILQVLFISVLTGAALSIGGKKDSPILRGIEEGQDVLFRILGFIMRVAPIGAFGAIAAAVGAHGSGTLIYLAKLV
GLYYVTSLAFVFLVLGSVAGMAGLSILKLIRLVREELLLVLGTASGEVALPRLMQKLEKAGCDEAVVGFVLPAGYSFNLD
GTAIYMAIAVGFIAQATDTPFSLGQQIGVLAIMLLTSKGGTTVAGGAFIKLAATLQTVRALPLNGLGLLFGVDRLMATCT
ALTNVVGNTVAVFVIARWENAFNRTKFDSYMAELAAPAGAGPQASAVAVPASAKAGE
>Mature_457_residues
MRPNSKAAGSSGGSTGVKFVMARLRQLYVQVIIAILLGIALGILAPATAVEMKPLGDAFIGLLRMMLGPIIFCSVVLGLT
HVRDMGQLGRLAFKALLYFEILTTVGMALGFVVVNVFEPGAGLHATSLAVNESVARISNTASHFTAVGFFLSIIPNTLVD
AFAKGEILQVLFISVLTGAALSIGGKKDSPILRGIEEGQDVLFRILGFIMRVAPIGAFGAIAAAVGAHGSGTLIYLAKLV
GLYYVTSLAFVFLVLGSVAGMAGLSILKLIRLVREELLLVLGTASGEVALPRLMQKLEKAGCDEAVVGFVLPAGYSFNLD
GTAIYMAIAVGFIAQATDTPFSLGQQIGVLAIMLLTSKGGTTVAGGAFIKLAATLQTVRALPLNGLGLLFGVDRLMATCT
ALTNVVGNTVAVFVIARWENAFNRTKFDSYMAELAAPAGAGPQASAVAVPASAKAGE

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=474, Percent_Identity=25.1054852320675, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI21314632, Length=401, Percent_Identity=24.1895261845387, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI40254478, Length=420, Percent_Identity=24.7619047619048, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI5032093, Length=412, Percent_Identity=25.7281553398058, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI169790839, Length=137, Percent_Identity=32.8467153284672, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI223468564, Length=283, Percent_Identity=25.4416961130742, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI194239697, Length=266, Percent_Identity=26.6917293233083, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI301601644, Length=150, Percent_Identity=28, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI223468566, Length=283, Percent_Identity=25.4416961130742, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789947, Length=424, Percent_Identity=46.6981132075472, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1790514, Length=433, Percent_Identity=38.7990762124711, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1788024, Length=405, Percent_Identity=24.1975308641975, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71996953, Length=462, Percent_Identity=25.7575757575758, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17537407, Length=385, Percent_Identity=26.7532467532467, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI193206505, Length=440, Percent_Identity=26.8181818181818, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193206654, Length=455, Percent_Identity=24.3956043956044, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71983099, Length=433, Percent_Identity=23.5565819861432, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71983106, Length=433, Percent_Identity=23.5565819861432, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17541374, Length=395, Percent_Identity=24.3037974683544, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583025, Length=388, Percent_Identity=22.4226804123711, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17137668, Length=388, Percent_Identity=22.4226804123711, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583023, Length=388, Percent_Identity=22.4226804123711, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 47279; Mature: 47279

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MRPNSKAAGSSGGSTGVKFVMARLRQLYVQVIIAILLGIALGILAPATAVEMKPLGDAFI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHH
GLLRMMLGPIIFCSVVLGLTHVRDMGQLGRLAFKALLYFEILTTVGMALGFVVVNVFEPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGLHATSLAVNESVARISNTASHFTAVGFFLSIIPNTLVDAFAKGEILQVLFISVLTGAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LSIGGKKDSPILRGIEEGQDVLFRILGFIMRVAPIGAFGAIAAAVGAHGSGTLIYLAKLV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHH
GLYYVTSLAFVFLVLGSVAGMAGLSILKLIRLVREELLLVLGTASGEVALPRLMQKLEKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GCDEAVVGFVLPAGYSFNLDGTAIYMAIAVGFIAQATDTPFSLGQQIGVLAIMLLTSKGG
CCCHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
TTVAGGAFIKLAATLQTVRALPLNGLGLLFGVDRLMATCTALTNVVGNTVAVFVIARWEN
CEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEHHHHH
AFNRTKFDSYMAELAAPAGAGPQASAVAVPASAKAGE
HHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA