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The map label for this gene is lysR [H]

Identifier: 158422948

GI number: 158422948

Start: 1482138

End: 1483046

Strand: Direct

Name: lysR [H]

Synonym: AZC_1324

Alternate gene names: 158422948

Gene position: 1482138-1483046 (Clockwise)

Preceding gene: 158422940

Following gene: 158422952

Centisome position: 27.6

GC content: 68.1

Gene sequence:

>909_bases
GTGGCGCGGGCTGGAAGCGTCAGCCGCGCCTCGGTCATCCTGAACGTCGCGCAACCCGCCCTCAGCCGCCAGTTGATGAA
GCTGGAGGAGGAGCTCGGCGTCAGCCTGTTCGTGCGTCACGGGCGCGGCGTGCGGCTGACCCGCGCCGGCAGCTCGCTGC
TGGAGCACGCCAACGCCGTGCTGCGGCAGGTGGACCAGATCTCGGAACTCGTGCGGGCGCCGGAGACGAGCTTCGCCGGC
CATGTGGTGCTCGGCGTGCCGCCGGCGGCAGGCCTCATCATCGCTCCGGTGGTGGTGGAAATCCTGCGCGAACGCTGGCC
GCATGCCTCGCTCCACGTGCGCGAGGGCATCAGTTCCTCTCTGGAGGAATGGCTGCTCGACCGCCGCCTCGATCTCGCGA
TCCTCTATAATCCGCCGCCGCTGGACGGCATCGTCCTGACGCCGATCCTGCACGAGCGGATGGTGGCCGCCGGTCCCATT
ACCGAGAGTCAGATGAGCGCGCGTACCATCAACTGGCGGGACCTTTCCGGCCTGCCGCTGATCCTGCCGTCTCTGCCCCA
CAGCAACCGCCGCCTGATCGAACAGGCGGCCATCCAGCGTGGCGCCCGCCTCGACGTGGTGTTCGAGGTGGACAGCGTCA
CCATGACCAAGGCCATGATCAAGCGGGGCCTTGGCTGGACCATTCTGGCCCATGCTGCGGTGGCGGGGGAGGCGGAGCGG
GGAGAATTGCAGGTGCGGGTCATCGACCGGCCCCCGCTCATCTCCACGGTCTCCATCGGCATCCCGACGGAAACCCAGTC
CACGCCGCTTGCCCATGAGCTCTTCGATGTGGTGCGCGGCGTCATCGTGGAGCGTGTGGCTGCAGGCGCGTGGTACGGCG
CCAAGGTGGTAGATGACTATGTCCGCTGA

Upstream 100 bases:

>100_bases
AGGTGCCTGCCAAAACGGCGTCCATCACGAGCGTCCGTGCGGCGGCAACCGCCGATGGAGGTGCGCCATCGACATCCGTG
GACTTCGCTATTTCGTCCAG

Downstream 100 bases:

>100_bases
CATGCGGGTTTCACTCACCGGCCTTGGCGGATGCGGGGACCGCCACGGCCGACGCCTGCGGGCCGGCACCGGCCGGCGCG
GCGAGCTCGGCCATGTAGCT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MARAGSVSRASVILNVAQPALSRQLMKLEEELGVSLFVRHGRGVRLTRAGSSLLEHANAVLRQVDQISELVRAPETSFAG
HVVLGVPPAAGLIIAPVVVEILRERWPHASLHVREGISSSLEEWLLDRRLDLAILYNPPPLDGIVLTPILHERMVAAGPI
TESQMSARTINWRDLSGLPLILPSLPHSNRRLIEQAAIQRGARLDVVFEVDSVTMTKAMIKRGLGWTILAHAAVAGEAER
GELQVRVIDRPPLISTVSIGIPTETQSTPLAHELFDVVRGVIVERVAAGAWYGAKVVDDYVR

Sequences:

>Translated_302_residues
MARAGSVSRASVILNVAQPALSRQLMKLEEELGVSLFVRHGRGVRLTRAGSSLLEHANAVLRQVDQISELVRAPETSFAG
HVVLGVPPAAGLIIAPVVVEILRERWPHASLHVREGISSSLEEWLLDRRLDLAILYNPPPLDGIVLTPILHERMVAAGPI
TESQMSARTINWRDLSGLPLILPSLPHSNRRLIEQAAIQRGARLDVVFEVDSVTMTKAMIKRGLGWTILAHAAVAGEAER
GELQVRVIDRPPLISTVSIGIPTETQSTPLAHELFDVVRGVIVERVAAGAWYGAKVVDDYVR
>Mature_301_residues
ARAGSVSRASVILNVAQPALSRQLMKLEEELGVSLFVRHGRGVRLTRAGSSLLEHANAVLRQVDQISELVRAPETSFAGH
VVLGVPPAAGLIIAPVVVEILRERWPHASLHVREGISSSLEEWLLDRRLDLAILYNPPPLDGIVLTPILHERMVAAGPIT
ESQMSARTINWRDLSGLPLILPSLPHSNRRLIEQAAIQRGARLDVVFEVDSVTMTKAMIKRGLGWTILAHAAVAGEAERG
ELQVRVIDRPPLISTVSIGIPTETQSTPLAHELFDVVRGVIVERVAAGAWYGAKVVDDYVR

Specific function: Transcriptional Activator For The Hut, Put And Ure Operons And Repressor For The Gdh And Gltb Operons In Response To Nitrogen Limitation. Negative Regulator Of Its Own Expression (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=231, Percent_Identity=30.7359307359307, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693105, Length=229, Percent_Identity=29.6943231441048, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790399, Length=240, Percent_Identity=28.3333333333333, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787601, Length=157, Percent_Identity=32.484076433121, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=248, Percent_Identity=29.4354838709677, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788481, Length=255, Percent_Identity=25.0980392156863, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787530, Length=277, Percent_Identity=25.2707581227437, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788887, Length=234, Percent_Identity=26.0683760683761, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789506, Length=213, Percent_Identity=24.8826291079812, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI2367136, Length=236, Percent_Identity=30.9322033898305, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=229, Percent_Identity=25.3275109170306, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=24.1803278688525, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787879, Length=225, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787806, Length=225, Percent_Identity=24.8888888888889, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32920; Mature: 32789

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAGSVSRASVILNVAQPALSRQLMKLEEELGVSLFVRHGRGVRLTRAGSSLLEHANAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEHHHHHHHHHHHHH
LRQVDQISELVRAPETSFAGHVVLGVPPAAGLIIAPVVVEILRERWPHASLHVREGISSS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LEEWLLDRRLDLAILYNPPPLDGIVLTPILHERMVAAGPITESQMSARTINWRDLSGLPL
HHHHHHHCCCCEEEEECCCCCCCEEECHHHHHHHHHCCCCCHHHHHHEECCCCCCCCCCE
ILPSLPHSNRRLIEQAAIQRGARLDVVFEVDSVTMTKAMIKRGLGWTILAHAAVAGEAER
EECCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCC
GELQVRVIDRPPLISTVSIGIPTETQSTPLAHELFDVVRGVIVERVAAGAWYGAKVVDDY
CCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VR
CC
>Mature Secondary Structure 
ARAGSVSRASVILNVAQPALSRQLMKLEEELGVSLFVRHGRGVRLTRAGSSLLEHANAV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEHHHHHHHHHHHHH
LRQVDQISELVRAPETSFAGHVVLGVPPAAGLIIAPVVVEILRERWPHASLHVREGISSS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LEEWLLDRRLDLAILYNPPPLDGIVLTPILHERMVAAGPITESQMSARTINWRDLSGLPL
HHHHHHHCCCCEEEEECCCCCCCEEECHHHHHHHHHCCCCCHHHHHHEECCCCCCCCCCE
ILPSLPHSNRRLIEQAAIQRGARLDVVFEVDSVTMTKAMIKRGLGWTILAHAAVAGEAER
EECCCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCC
GELQVRVIDRPPLISTVSIGIPTETQSTPLAHELFDVVRGVIVERVAAGAWYGAKVVDDY
CCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9524276; 12910271 [H]