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The map label for this gene is rimO

Identifier: 158422878

GI number: 158422878

Start: 1402899

End: 1404251

Strand: Reverse

Name: rimO

Synonym: AZC_1254

Alternate gene names: 158422878

Gene position: 1404251-1402899 (Counterclockwise)

Preceding gene: 158422880

Following gene: 158422876

Centisome position: 26.15

GC content: 66.96

Gene sequence:

>1353_bases
ATGGCCGAACCCACCAGCACGCTCTCGACCGCAACCCCGGCGGCGCCCCGCATCTCCTTCGTTTCGCTGGGATGCCCCAA
GGCTCTGGTGGACAGCGAGCGCATCGTCACGCGCCTCAGGGCGGAAGGCTATGAGCTGACGCGCAACCACGACGGCGCCG
ATCTCGTCATCGTGAACACCTGCGGCTTCCTCGACAGCGCCAAGGCGGAAAGCCTCTCGGCCATCGGCGAGGCGCTGGCG
GAGAACGGCAAGGTGGTCGTGACCGGCTGCATGGGCGCCGAGCCGGAGCAGATCCGCGCCGTCCACCCGAGCGTGCTCGC
CATCACCGGCCCGCAGCAATATGAAAGCGTGCTTGACGCCGTGCACCAGGCGGTGCCGCCCAAGCACGATCCCTTCCTGG
ACCTCGTGCCCGAACAGGGCGTGAAGCTCACGCCGCGCCACTACGCCTATCTGAAGATTTCAGAAGGCTGCAACAACCGC
TGCACCTTCTGCATCATCCCGAAGCTGCGCGGCGATCTCGTGAGCCGCCCGCTGAACGAGGTGATGCGCGAGGCGGAAAA
GCTGGTGGCGGCCGGCGTGAAGGAACTGCTCGTCATCTCGCAGGACACCTCGGCCTATGGCGTCGACCTGAAATATGCCG
AGAGCCGCTGGAAGGACCGCGACTGGGCGACCCGCTTCTTTGATCTGGCCGATGCGCTGGGCGATCTCGGCGCCTGGGTG
CGCCTCCATTACGTCTATCCCTATCCGCACGTGGACCGGGTGATGGAGCTGATGGCGAGCGGCAAGGTGCTGCCCTATCT
CGACATTCCCTTCCAGCACGCCAGCCCCACCGTGCTGCGGCGGATGAAGCGTCCGGCGGCGCAGGAGAAGACGCTGGCCC
GCGTGCTGGCGTGGCGGGATGCGGTGCCCGACATCACCCTGCGCTCCACCTTCATCGTCGGCTTCCCCGGCGAGACCGAG
GAGGAGTTCCAGGAACTGCTGGACTTCCTCGACGAAGCACAGCTCGACCGCGTCGGTTGCTTCAAGTTCGAGCCGGTGGC
GGGCGCACCCGCCAATGCGCTGGAAAATCCGGTGCCGGACGAGGTGAAGGCCGAGCGCTACGACCGCTTCATGCTGAAGC
AGCAGGCAATCTCCGCCCGCCGCCTCAAGCGTAAGGTGGGCACCCGCCAGCAGGTCATCATCGACAGCGTGACGCCCGGC
GGCGCCATCGGCCGCACCAAGGGCGATGCGCCGGAGATCGACGGCTCGGTGAAGATCGCCTCCCGGCGCCCGCTGCGCGT
GGGCGAGATCGCCACGGTGAAGATCGAGGCGGCGGACGCCTATGATCTGATCGGCTCTGCCGTCGGCTTCTGA

Upstream 100 bases:

>100_bases
CCCGCGCCGGGCAGCCATGCGGCAGCGGGATTGACGGGCGGGTCAACCTCCCGTAACGCCTCTGCGCTCTCTGATACCAA
ACGGACCTGCGCGCCACATC

Downstream 100 bases:

>100_bases
CGCGGCGGCTACCCGCCGCCCCTTGAAATACCCGACACAGATACCCTCTCGTCCAGCCTTAAGCGGAGAGGGACGCGTCA
TCGCGTCCCAAGGCGGATGC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAEPTSTLSTATPAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTRNHDGADLVIVNTCGFLDSAKAESLSAIGEALA
ENGKVVVTGCMGAEPEQIRAVHPSVLAITGPQQYESVLDAVHQAVPPKHDPFLDLVPEQGVKLTPRHYAYLKISEGCNNR
CTFCIIPKLRGDLVSRPLNEVMREAEKLVAAGVKELLVISQDTSAYGVDLKYAESRWKDRDWATRFFDLADALGDLGAWV
RLHYVYPYPHVDRVMELMASGKVLPYLDIPFQHASPTVLRRMKRPAAQEKTLARVLAWRDAVPDITLRSTFIVGFPGETE
EEFQELLDFLDEAQLDRVGCFKFEPVAGAPANALENPVPDEVKAERYDRFMLKQQAISARRLKRKVGTRQQVIIDSVTPG
GAIGRTKGDAPEIDGSVKIASRRPLRVGEIATVKIEAADAYDLIGSAVGF

Sequences:

>Translated_450_residues
MAEPTSTLSTATPAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTRNHDGADLVIVNTCGFLDSAKAESLSAIGEALA
ENGKVVVTGCMGAEPEQIRAVHPSVLAITGPQQYESVLDAVHQAVPPKHDPFLDLVPEQGVKLTPRHYAYLKISEGCNNR
CTFCIIPKLRGDLVSRPLNEVMREAEKLVAAGVKELLVISQDTSAYGVDLKYAESRWKDRDWATRFFDLADALGDLGAWV
RLHYVYPYPHVDRVMELMASGKVLPYLDIPFQHASPTVLRRMKRPAAQEKTLARVLAWRDAVPDITLRSTFIVGFPGETE
EEFQELLDFLDEAQLDRVGCFKFEPVAGAPANALENPVPDEVKAERYDRFMLKQQAISARRLKRKVGTRQQVIIDSVTPG
GAIGRTKGDAPEIDGSVKIASRRPLRVGEIATVKIEAADAYDLIGSAVGF
>Mature_449_residues
AEPTSTLSTATPAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTRNHDGADLVIVNTCGFLDSAKAESLSAIGEALAE
NGKVVVTGCMGAEPEQIRAVHPSVLAITGPQQYESVLDAVHQAVPPKHDPFLDLVPEQGVKLTPRHYAYLKISEGCNNRC
TFCIIPKLRGDLVSRPLNEVMREAEKLVAAGVKELLVISQDTSAYGVDLKYAESRWKDRDWATRFFDLADALGDLGAWVR
LHYVYPYPHVDRVMELMASGKVLPYLDIPFQHASPTVLRRMKRPAAQEKTLARVLAWRDAVPDITLRSTFIVGFPGETEE
EFQELLDFLDEAQLDRVGCFKFEPVAGAPANALENPVPDEVKAERYDRFMLKQQAISARRLKRKVGTRQQVIIDSVTPGG
AIGRTKGDAPEIDGSVKIASRRPLRVGEIATVKIEAADAYDLIGSAVGF

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=400, Percent_Identity=29.75, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=24.6346555323591, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=24.6346555323591, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1787057, Length=442, Percent_Identity=65.6108597285068, Blast_Score=592, Evalue=1e-170,
Organism=Escherichia coli, GI1786882, Length=416, Percent_Identity=28.125, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=476, Percent_Identity=25, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71996771, Length=306, Percent_Identity=27.1241830065359, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=25.5765199161426, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922432, Length=384, Percent_Identity=25.78125, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_AZOC5 (A8I0G1)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001524170.1
- ProteinModelPortal:   A8I0G1
- SMR:   A8I0G1
- GeneID:   5691116
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_1254
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- BioCyc:   ACAU438753:AZC_1254-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49432; Mature: 49301

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPTSTLSTATPAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTRNHDGADLVIVNT
CCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEE
CGFLDSAKAESLSAIGEALAENGKVVVTGCMGAEPEQIRAVHPSVLAITGPQQYESVLDA
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEEEEECHHHHHHHHHH
VHQAVPPKHDPFLDLVPEQGVKLTPRHYAYLKISEGCNNRCTFCIIPKLRGDLVSRPLNE
HHHHCCCCCCCCEECCCCCCCEECCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHH
VMREAEKLVAAGVKELLVISQDTSAYGVDLKYAESRWKDRDWATRFFDLADALGDLGAWV
HHHHHHHHHHHCHHHHEEEECCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHCCEE
RLHYVYPYPHVDRVMELMASGKVLPYLDIPFQHASPTVLRRMKRPAAQEKTLARVLAWRD
EEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
AVPDITLRSTFIVGFPGETEEEFQELLDFLDEAQLDRVGCFKFEPVAGAPANALENPVPD
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHCCCCCC
EVKAERYDRFMLKQQAISARRLKRKVGTRQQVIIDSVTPGGAIGRTKGDAPEIDGSVKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEC
SRRPLRVGEIATVKIEAADAYDLIGSAVGF
CCCCCEECCEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
AEPTSTLSTATPAAPRISFVSLGCPKALVDSERIVTRLRAEGYELTRNHDGADLVIVNT
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEE
CGFLDSAKAESLSAIGEALAENGKVVVTGCMGAEPEQIRAVHPSVLAITGPQQYESVLDA
CCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEEEEECHHHHHHHHHH
VHQAVPPKHDPFLDLVPEQGVKLTPRHYAYLKISEGCNNRCTFCIIPKLRGDLVSRPLNE
HHHHCCCCCCCCEECCCCCCCEECCCEEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHH
VMREAEKLVAAGVKELLVISQDTSAYGVDLKYAESRWKDRDWATRFFDLADALGDLGAWV
HHHHHHHHHHHCHHHHEEEECCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHCCEE
RLHYVYPYPHVDRVMELMASGKVLPYLDIPFQHASPTVLRRMKRPAAQEKTLARVLAWRD
EEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
AVPDITLRSTFIVGFPGETEEEFQELLDFLDEAQLDRVGCFKFEPVAGAPANALENPVPD
CCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHCCCCCC
EVKAERYDRFMLKQQAISARRLKRKVGTRQQVIIDSVTPGGAIGRTKGDAPEIDGSVKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEC
SRRPLRVGEIATVKIEAADAYDLIGSAVGF
CCCCCEECCEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA