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The map label for this gene is potB [H]

Identifier: 158422857

GI number: 158422857

Start: 1383108

End: 1384004

Strand: Direct

Name: potB [H]

Synonym: AZC_1233

Alternate gene names: 158422857

Gene position: 1383108-1384004 (Clockwise)

Preceding gene: 158422856

Following gene: 158422858

Centisome position: 25.76

GC content: 68.12

Gene sequence:

>897_bases
ATGAGCGCGGTTGCCCATCTCGCCCCCGCCCGCGAGGGGGCGCCCGCCTGGATGGCCCCGGCGCTCATGCTGCTGCCGGC
GCTGCTCATCGTGTTCGGCGTGGTGCTGGTGCCGCTGGTCATGACGGCGCTCCTGTCCTTCCATTCCTTCTCCAGCTCCA
CCGGCATCTCGCCGGGCCTGTCGCTGGAGAACTGGGCCGAGATCGCCGCCGATCCCTATTATGGCGAGATGTTCCTGCGC
ACCTTCCGCATCGCCGGTGTGGTGACGCTGGCCTGCTGCCTCATCGGCGCGCCGGAGGCCTATGTCATCCACCGCATGAA
GGCGCCCTGGAAGGGCATCTTCCTGGTGGTCACGCTTGGTCCGCTGCTCATTTCCGTGGTGGCGCGCACCCTTGGCTGGG
CGCTCCTGTTCGGCGGCCGCAACGGCGTCGTCAACAAGGCGCTGATGGACCTCGGCGTGCTCTCCCAGCCCATCCCCTTC
ATGTTCACCGAGGGTGGGGTGATGGTGGCGCTCACCCATGTGATGGTGCCCTTCATGGTGCTCTCCGTCTGGGCCGTGCT
GCAGCGGCTCGACCCGCGCACGGAGAATGCCGCCGCCTCGCTCGGCGCCTCGCAATTCACCATCCTCACCCGCATCGTGC
TGCCGCAGGTGCTGCCCGGCATCCTGTCCGGCGCCATAATCGTCTTCGCCCTGTCGGCGAGCTCCTTCGCCACCCCCGCC
ATCATCGGCGGCCGGCGGCTGAAGGTGGCGGCGACGCTGGCCTATGACGAGTTCCTCAACACGCTCAACTGGCCGCTCGG
CGCCGCCGTGGCGGTGCTGCTGCTCGCCGCCATCGCCGCCATCGTCATCGGCTGCAACACGTTGGTGGAGCGGCGTTATG
CGAGGATGACCCAATGA

Upstream 100 bases:

>100_bases
TCGTGTGCCAGAATGACGGCGCGCCGAAGCCGGAGGAGGGCGAGAGCGTCCATCTCTCCTGGCGCCCGGAGGATGCCGGC
CTGCGGGCGGGAGAGGGGCG

Downstream 100 bases:

>100_bases
GCCGCAACGGTCCCCTCGCGCTCGTCTTCCATGCGCTGGTCATGGTGTTCATGCTGGCGCCCATCCTCATCGTGTGCGTG
GTCGCCTTCACGCCCGAGGG

Product: ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSAVAHLAPAREGAPAWMAPALMLLPALLIVFGVVLVPLVMTALLSFHSFSSSTGISPGLSLENWAEIAADPYYGEMFLR
TFRIAGVVTLACCLIGAPEAYVIHRMKAPWKGIFLVVTLGPLLISVVARTLGWALLFGGRNGVVNKALMDLGVLSQPIPF
MFTEGGVMVALTHVMVPFMVLSVWAVLQRLDPRTENAAASLGASQFTILTRIVLPQVLPGILSGAIIVFALSASSFATPA
IIGGRRLKVAATLAYDEFLNTLNWPLGAAVAVLLLAAIAAIVIGCNTLVERRYARMTQ

Sequences:

>Translated_298_residues
MSAVAHLAPAREGAPAWMAPALMLLPALLIVFGVVLVPLVMTALLSFHSFSSSTGISPGLSLENWAEIAADPYYGEMFLR
TFRIAGVVTLACCLIGAPEAYVIHRMKAPWKGIFLVVTLGPLLISVVARTLGWALLFGGRNGVVNKALMDLGVLSQPIPF
MFTEGGVMVALTHVMVPFMVLSVWAVLQRLDPRTENAAASLGASQFTILTRIVLPQVLPGILSGAIIVFALSASSFATPA
IIGGRRLKVAATLAYDEFLNTLNWPLGAAVAVLLLAAIAAIVIGCNTLVERRYARMTQ
>Mature_297_residues
SAVAHLAPAREGAPAWMAPALMLLPALLIVFGVVLVPLVMTALLSFHSFSSSTGISPGLSLENWAEIAADPYYGEMFLRT
FRIAGVVTLACCLIGAPEAYVIHRMKAPWKGIFLVVTLGPLLISVVARTLGWALLFGGRNGVVNKALMDLGVLSQPIPFM
FTEGGVMVALTHVMVPFMVLSVWAVLQRLDPRTENAAASLGASQFTILTRIVLPQVLPGILSGAIIVFALSASSFATPAI
IGGRRLKVAATLAYDEFLNTLNWPLGAAVAVLLLAAIAAIVIGCNTLVERRYARMTQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=262, Percent_Identity=29.3893129770992, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=30.9090909090909, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787713, Length=228, Percent_Identity=29.8245614035088, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788764, Length=239, Percent_Identity=25.1046025104602, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31622; Mature: 31491

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVAHLAPAREGAPAWMAPALMLLPALLIVFGVVLVPLVMTALLSFHSFSSSTGISPGL
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SLENWAEIAADPYYGEMFLRTFRIAGVVTLACCLIGAPEAYVIHRMKAPWKGIFLVVTLG
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHH
PLLISVVARTLGWALLFGGRNGVVNKALMDLGVLSQPIPFMFTEGGVMVALTHVMVPFMV
HHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHH
LSVWAVLQRLDPRTENAAASLGASQFTILTRIVLPQVLPGILSGAIIVFALSASSFATPA
HHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
IIGGRRLKVAATLAYDEFLNTLNWPLGAAVAVLLLAAIAAIVIGCNTLVERRYARMTQ
HCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAVAHLAPAREGAPAWMAPALMLLPALLIVFGVVLVPLVMTALLSFHSFSSSTGISPGL
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SLENWAEIAADPYYGEMFLRTFRIAGVVTLACCLIGAPEAYVIHRMKAPWKGIFLVVTLG
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHH
PLLISVVARTLGWALLFGGRNGVVNKALMDLGVLSQPIPFMFTEGGVMVALTHVMVPFMV
HHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHH
LSVWAVLQRLDPRTENAAASLGASQFTILTRIVLPQVLPGILSGAIIVFALSASSFATPA
HHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
IIGGRRLKVAATLAYDEFLNTLNWPLGAAVAVLLLAAIAAIVIGCNTLVERRYARMTQ
HCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]