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The map label for this gene is dosC [H]

Identifier: 158422828

GI number: 158422828

Start: 1345398

End: 1346606

Strand: Reverse

Name: dosC [H]

Synonym: AZC_1204

Alternate gene names: 158422828

Gene position: 1346606-1345398 (Counterclockwise)

Preceding gene: 158422833

Following gene: 158422824

Centisome position: 25.08

GC content: 68.16

Gene sequence:

>1209_bases
ATGGTCACCCTCGATTTCCAGACGATAGGCTTCGCCGCTGTGGTGCTGGCCACCCTCATGGGTGCGCTGCTGCTGCTGGC
GTGGGCCTATGTCCGGAGCGAGACCTGCCTCATCTTCTGGGGTGTCACGGACCTGTTCTGTGCCGGCTCGGTCGCCGTCC
TGACCGCCGCCAACGGGCGTCTGGGGCCGAACGTGGCGCCCTTCAGCAACGGCTGGCTTCTGACGGGGGCCGCCCTCACC
TATTGCGGCATGCGCGCCTTCGACCGGCGCCCGATCACGCTGCCCATCTGGCTGGCGGTGATCGCCACGCCCCTCTTCTA
TTATATTGCGTGCGTGCTGTTCCTGCCCAACGTGAACGACAGGGTGGTGCTCTACACCTTCATCTGCTGTTGCTGGTTCG
GCGGCTCCGCCTACGTGCTCGCCCACGTGCCGTCCTATGCGCCGGCGTTTTCCCGCATGCTGGTCGCCCTCTTCCTTGCC
GGCATGTGCACCATACAGGTGGTGCGGCTCGCAGGCAGTCTCGCCGGCTTCCGGCCGCTCGGCGGCGACATGACATCAGA
GCAGATGGCCTGGACCTTCGCCGCCGGCCTCTTCTTCACCGTAGGGCTGAACTTCGGCGGCATGTTCATGGTGCTGGACC
GCATGGCCAGCTGTGACGAACTGACCGAACTCAGCAACCGCCGTTCGCTGCTCATGGGCGCCAGCGCCCTGCTGCGCTCG
GCCCTTGCCTCGGGGCGTCCGGTCTCGGTGCTGCTGGTGGATCTCGATCACTTCAAGTCCATCAACGACCGCTTCGGCCA
CCGGACCGGCGATCAGGTGCTGCGGGCCTTCGCGCAGGTTGCGCGCAAGCTGCTGCGCCGGGGCGATCTCGTGGGGCGCT
ATGGCGGCGAGGAATTCTGCCTCGTGCTCAAGGATGCCGGATCGGATGTGGCCTGCGTAATGGCGGAGCGCCTGCGGCGG
CAGGTAATGGACGATCTGCGGGAAGTGGAAGGCCATGCGGTGGAGGCCACGGTCGCGATCGGCGTCGCAAGCCTGGAGGC
CGGGCAGCGCGTGGCCGGCATCGACCGCATGATCGATGCCGCGGACCATGCGCTCTATGCCGCCAAGGACGGAGGCCGCA
ACCGGGTGGTGCTCGCGGCGCCGCTCGTCACGCCCCAGCCGGCGGCCCCGCACGCCGGGGGCGCCGCCGCCTCCACGGGC
GTCGCCTGA

Upstream 100 bases:

>100_bases
GGCAGGCCCAGCGGCGACGAGCTTTTCCTTGCCGCCCCAGAACAGCCGCGCAATGGTATCAGGATTGCGCAATAGCCTGA
TTGTCTCGGCCCCCCGGATT

Downstream 100 bases:

>100_bases
GCTTCTCGCGTCACTCCGGCAAAAGGCGTCCCGAATAGAGCACGTCGCCGCTCGGCAATTCGGATGTGCCGCCTTCCGGC
TCCAGCGTCACCTGGAGCGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MVTLDFQTIGFAAVVLATLMGALLLLAWAYVRSETCLIFWGVTDLFCAGSVAVLTAANGRLGPNVAPFSNGWLLTGAALT
YCGMRAFDRRPITLPIWLAVIATPLFYYIACVLFLPNVNDRVVLYTFICCCWFGGSAYVLAHVPSYAPAFSRMLVALFLA
GMCTIQVVRLAGSLAGFRPLGGDMTSEQMAWTFAAGLFFTVGLNFGGMFMVLDRMASCDELTELSNRRSLLMGASALLRS
ALASGRPVSVLLVDLDHFKSINDRFGHRTGDQVLRAFAQVARKLLRRGDLVGRYGGEEFCLVLKDAGSDVACVMAERLRR
QVMDDLREVEGHAVEATVAIGVASLEAGQRVAGIDRMIDAADHALYAAKDGGRNRVVLAAPLVTPQPAAPHAGGAAASTG
VA

Sequences:

>Translated_402_residues
MVTLDFQTIGFAAVVLATLMGALLLLAWAYVRSETCLIFWGVTDLFCAGSVAVLTAANGRLGPNVAPFSNGWLLTGAALT
YCGMRAFDRRPITLPIWLAVIATPLFYYIACVLFLPNVNDRVVLYTFICCCWFGGSAYVLAHVPSYAPAFSRMLVALFLA
GMCTIQVVRLAGSLAGFRPLGGDMTSEQMAWTFAAGLFFTVGLNFGGMFMVLDRMASCDELTELSNRRSLLMGASALLRS
ALASGRPVSVLLVDLDHFKSINDRFGHRTGDQVLRAFAQVARKLLRRGDLVGRYGGEEFCLVLKDAGSDVACVMAERLRR
QVMDDLREVEGHAVEATVAIGVASLEAGQRVAGIDRMIDAADHALYAAKDGGRNRVVLAAPLVTPQPAAPHAGGAAASTG
VA
>Mature_402_residues
MVTLDFQTIGFAAVVLATLMGALLLLAWAYVRSETCLIFWGVTDLFCAGSVAVLTAANGRLGPNVAPFSNGWLLTGAALT
YCGMRAFDRRPITLPIWLAVIATPLFYYIACVLFLPNVNDRVVLYTFICCCWFGGSAYVLAHVPSYAPAFSRMLVALFLA
GMCTIQVVRLAGSLAGFRPLGGDMTSEQMAWTFAAGLFFTVGLNFGGMFMVLDRMASCDELTELSNRRSLLMGASALLRS
ALASGRPVSVLLVDLDHFKSINDRFGHRTGDQVLRAFAQVARKLLRRGDLVGRYGGEEFCLVLKDAGSDVACVMAERLRR
QVMDDLREVEGHAVEATVAIGVASLEAGQRVAGIDRMIDAADHALYAAKDGGRNRVVLAAPLVTPQPAAPHAGGAAASTG
VA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=40.7407407407407, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787262, Length=168, Percent_Identity=34.5238095238095, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=207, Percent_Identity=36.7149758454106, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=41.0714285714286, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788381, Length=191, Percent_Identity=34.5549738219895, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=32.3699421965318, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787802, Length=162, Percent_Identity=35.8024691358025, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=31.3609467455621, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=188, Percent_Identity=35.6382978723404, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=31.1377245508982, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=186, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788956, Length=171, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=31.1764705882353, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42959; Mature: 42959

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLDFQTIGFAAVVLATLMGALLLLAWAYVRSETCLIFWGVTDLFCAGSVAVLTAANGR
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCEEEEEECCCC
LGPNVAPFSNGWLLTGAALTYCGMRAFDRRPITLPIWLAVIATPLFYYIACVLFLPNVND
CCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
RVVLYTFICCCWFGGSAYVLAHVPSYAPAFSRMLVALFLAGMCTIQVVRLAGSLAGFRPL
CHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGDMTSEQMAWTFAAGLFFTVGLNFGGMFMVLDRMASCDELTELSNRRSLLMGASALLRS
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ALASGRPVSVLLVDLDHFKSINDRFGHRTGDQVLRAFAQVARKLLRRGDLVGRYGGEEFC
HHHCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEE
LVLKDAGSDVACVMAERLRRQVMDDLREVEGHAVEATVAIGVASLEAGQRVAGIDRMIDA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHH
ADHALYAAKDGGRNRVVLAAPLVTPQPAAPHAGGAAASTGVA
HHHHEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVTLDFQTIGFAAVVLATLMGALLLLAWAYVRSETCLIFWGVTDLFCAGSVAVLTAANGR
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCEEEEEECCCC
LGPNVAPFSNGWLLTGAALTYCGMRAFDRRPITLPIWLAVIATPLFYYIACVLFLPNVND
CCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
RVVLYTFICCCWFGGSAYVLAHVPSYAPAFSRMLVALFLAGMCTIQVVRLAGSLAGFRPL
CHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGDMTSEQMAWTFAAGLFFTVGLNFGGMFMVLDRMASCDELTELSNRRSLLMGASALLRS
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
ALASGRPVSVLLVDLDHFKSINDRFGHRTGDQVLRAFAQVARKLLRRGDLVGRYGGEEFC
HHHCCCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEE
LVLKDAGSDVACVMAERLRRQVMDDLREVEGHAVEATVAIGVASLEAGQRVAGIDRMIDA
EEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHHHHHHH
ADHALYAAKDGGRNRVVLAAPLVTPQPAAPHAGGAAASTGVA
HHHHEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]