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The map label for this gene is ybiS [H]

Identifier: 158422818

GI number: 158422818

Start: 1336650

End: 1337252

Strand: Reverse

Name: ybiS [H]

Synonym: AZC_1194

Alternate gene names: 158422818

Gene position: 1337252-1336650 (Counterclockwise)

Preceding gene: 158422820

Following gene: 158422815

Centisome position: 24.9

GC content: 64.68

Gene sequence:

>603_bases
ATGCGGGCGCGGGCGATTGCGGCTGTTTTGACGGGTATTGTGCTAGCGGGCTGCCAGACCAACCAGCTTCAACCGGCGCC
GGAGGCTTCCCTCAAGCCGCGCGACAAGCAGCTTCTCGCGAATGCGCCGTATCCGAAGGTGAAGCCGGAAGGCGAATTCG
CCCGTCAGATCGTGAGCTACTCCGGCAAGGAGAAGCCCGGCACCATCGTGGTCGATACCGACAAGCGCATGCTCTACTAC
GTGCAGGGCGACGGCACCGCGATCCGCTACGGCGTGACCGTGGGCGAAGAGAGCCTGGCGTTCCGTGGCGAAGCGAAGGT
GGGTCGCAAGGCCGAATGGCCGAGTTGGACCCCCACCCCGGAAATCCACCAGCGCATCGCCGGCCTGCCAAGTTACGTCA
ACGGCGGCCCGCAGAACCCGATGGGTGCCCGCGCGCTGTACCTCTATCAGGGCAACCGCGACACGCTCTTCCGCATCCAT
GGCACCAACCAGCCCGAACTGCTTGGTCAGGCCATCTCCTCCGGCTGCATCCGCATGCTGAACGAGGACGTGATCGATCT
GTTCAACCGCGTGCCCGTCGGCGCCAACGTGGTGGTTCTCTGA

Upstream 100 bases:

>100_bases
CATGGCAGCACGGTGGCCCCTTCAAGGCCCGGGAGGGTTAGGTTAATCCAATCCTGACATCTCGATTCGACGAGATTTTC
CAAACATTGAAGAGGCGGCC

Downstream 100 bases:

>100_bases
ACTGCACGCGCCGCGACGGCCTGAACAAGAAAACCCCGCCGGGCGCACCGGCGGGGTTTTTCTTTGGGTTGATGCCGCCA
GATACCTGCGCTCTGCCTCG

Product: ErfK/YbiS/YcfS/YnhG precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MRARAIAAVLTGIVLAGCQTNQLQPAPEASLKPRDKQLLANAPYPKVKPEGEFARQIVSYSGKEKPGTIVVDTDKRMLYY
VQGDGTAIRYGVTVGEESLAFRGEAKVGRKAEWPSWTPTPEIHQRIAGLPSYVNGGPQNPMGARALYLYQGNRDTLFRIH
GTNQPELLGQAISSGCIRMLNEDVIDLFNRVPVGANVVVL

Sequences:

>Translated_200_residues
MRARAIAAVLTGIVLAGCQTNQLQPAPEASLKPRDKQLLANAPYPKVKPEGEFARQIVSYSGKEKPGTIVVDTDKRMLYY
VQGDGTAIRYGVTVGEESLAFRGEAKVGRKAEWPSWTPTPEIHQRIAGLPSYVNGGPQNPMGARALYLYQGNRDTLFRIH
GTNQPELLGQAISSGCIRMLNEDVIDLFNRVPVGANVVVL
>Mature_200_residues
MRARAIAAVLTGIVLAGCQTNQLQPAPEASLKPRDKQLLANAPYPKVKPEGEFARQIVSYSGKEKPGTIVVDTDKRMLYY
VQGDGTAIRYGVTVGEESLAFRGEAKVGRKAEWPSWTPTPEIHQRIAGLPSYVNGGPQNPMGARALYLYQGNRDTLFRIH
GTNQPELLGQAISSGCIRMLNEDVIDLFNRVPVGANVVVL

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=137, Percent_Identity=39.4160583941606, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788299, Length=139, Percent_Identity=37.410071942446, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787968, Length=139, Percent_Identity=40.2877697841727, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787356, Length=143, Percent_Identity=32.1678321678322, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 21802; Mature: 21802

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARAIAAVLTGIVLAGCQTNQLQPAPEASLKPRDKQLLANAPYPKVKPEGEFARQIVSY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
SGKEKPGTIVVDTDKRMLYYVQGDGTAIRYGVTVGEESLAFRGEAKVGRKAEWPSWTPTP
CCCCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCH
EIHQRIAGLPSYVNGGPQNPMGARALYLYQGNRDTLFRIHGTNQPELLGQAISSGCIRML
HHHHHHHCCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
NEDVIDLFNRVPVGANVVVL
HHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MRARAIAAVLTGIVLAGCQTNQLQPAPEASLKPRDKQLLANAPYPKVKPEGEFARQIVSY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
SGKEKPGTIVVDTDKRMLYYVQGDGTAIRYGVTVGEESLAFRGEAKVGRKAEWPSWTPTP
CCCCCCCEEEEECCCEEEEEEECCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCH
EIHQRIAGLPSYVNGGPQNPMGARALYLYQGNRDTLFRIHGTNQPELLGQAISSGCIRML
HHHHHHHCCCHHHCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHH
NEDVIDLFNRVPVGANVVVL
HHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]