BacMap

Definition	Azorhizobium caulinodans ORS 571, complete genome.
Accession	NC_009937
Length	5,369,772

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The map label for this gene is fixR [H]

Identifier: 158422820

GI number: 158422820

Start: 1338898

End: 1339710

Strand: Reverse

Name: fixR [H]

Synonym: AZC_1196

Alternate gene names: 158422820

Gene position: 1339710-1338898 (Counterclockwise)

Preceding gene: 158422821

Following gene: 158422818

Centisome position: 24.95

GC content: 67.04

Gene sequence:

>813_bases
GTGCTGCCTGCGGTTCAAACAAGACGTTGCACCGCAGCATCCTCCCTTCCGAAAGTCCGCACCATGGTCGCCGTTTCCAA
CCAGCCGCCGCGCATCATGCTCCTGACCGGCGCCTCGCGCGGCATCGGCCACGCCACGGTCAAGCGCTTCTCCAGCGCCG
GCTGGCGCGTCATCACCTGCTCGCGCCAGCCGTTCCCGGAACAGTGCCCCTGGGCAGCCGGCCCCGAGGACCACATCCAG
GTGGATCTGGGCAATCCGGACGACACGCTGGCGGCGGTGGAGGAAATCAAGAACCGCCTGCCCAATGGCGAACTGCACGC
GCTCGTCAACAATGCCGGCATCTCGCCCAAGGGCGAGGGCGGCTCGCGGCTCGGCACGCTGGACACCCCCCACGACGTGT
GGATGCAGGTGTTCCAGGTGAATTTCTTCGCCTCCATCATGCTGGCGCGCGGGCTGGTCTCTGAACTGGAGAAGGCGCAG
GGCTCGGTTGTGAACGTCACCTCCATCGCCGGCTCGCGGGTGCATCCGTTCGCGGGTGCGGCCTATGCCACCTCGAAGGC
GGCCCTTGCGGCCCTGACCCGTGAGATGGCGGCGGACTTCGGGCCGCGCGGCGTGCGGGTGAACGCCATTGCGCCGGGCG
AGATCGACACGGCCATTCTTTCTCCGGGCACGGACAAGATCGTGGCGCAGATCCCCCTGCGGCGCCTCGGCACGCCGGAC
GAGGTGGCGAAGATCATCTACGTGCTGTGCACGGAGACCTCGTCCTATCTCAATGGCGCGGAAATCCTCATCAACGGCGG
TCAGCACGTCTGA

Upstream 100 bases:

>100_bases
TCCGGCTGCACGACCGCGTCGTCTTCAAGCGGGAAAATCCGGAAATCGACTGGGAAAAGAGCCGTCTTTATCCCTGATCC
TTGACCGTCGGGCGCTGCCC

Downstream 100 bases:

>100_bases
GCCGCCGAAGGCTCAGAGCCAGCGCCGCCATTTGAAGAAGAGGTAGGGCAGGATGCCCGACACCACCATGGCGCAGAGCG
CCAGGGGATAGCCGTAGGTC

Product: short-chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLPAVQTRRCTAASSLPKVRTMVAVSNQPPRIMLLTGASRGIGHATVKRFSSAGWRVITCSRQPFPEQCPWAAGPEDHIQ
VDLGNPDDTLAAVEEIKNRLPNGELHALVNNAGISPKGEGGSRLGTLDTPHDVWMQVFQVNFFASIMLARGLVSELEKAQ
GSVVNVTSIAGSRVHPFAGAAYATSKAALAALTREMAADFGPRGVRVNAIAPGEIDTAILSPGTDKIVAQIPLRRLGTPD
EVAKIIYVLCTETSSYLNGAEILINGGQHV

Sequences:

>Translated_270_residues
MLPAVQTRRCTAASSLPKVRTMVAVSNQPPRIMLLTGASRGIGHATVKRFSSAGWRVITCSRQPFPEQCPWAAGPEDHIQ
VDLGNPDDTLAAVEEIKNRLPNGELHALVNNAGISPKGEGGSRLGTLDTPHDVWMQVFQVNFFASIMLARGLVSELEKAQ
GSVVNVTSIAGSRVHPFAGAAYATSKAALAALTREMAADFGPRGVRVNAIAPGEIDTAILSPGTDKIVAQIPLRRLGTPD
EVAKIIYVLCTETSSYLNGAEILINGGQHV
>Mature_270_residues
MLPAVQTRRCTAASSLPKVRTMVAVSNQPPRIMLLTGASRGIGHATVKRFSSAGWRVITCSRQPFPEQCPWAAGPEDHIQ
VDLGNPDDTLAAVEEIKNRLPNGELHALVNNAGISPKGEGGSRLGTLDTPHDVWMQVFQVNFFASIMLARGLVSELEKAQ
GSVVNVTSIAGSRVHPFAGAAYATSKAALAALTREMAADFGPRGVRVNAIAPGEIDTAILSPGTDKIVAQIPLRRLGTPD
EVAKIIYVLCTETSSYLNGAEILINGGQHV

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=29.296875, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI59889578, Length=240, Percent_Identity=29.5833333333333, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI5031737, Length=248, Percent_Identity=29.4354838709677, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=29.3436293436293, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=27.8688524590164, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI126723750, Length=251, Percent_Identity=27.4900398406374, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI7705925, Length=252, Percent_Identity=29.7619047619048, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI20149619, Length=230, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI19923817, Length=286, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI119392083, Length=191, Percent_Identity=31.9371727748691, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4503301, Length=239, Percent_Identity=28.8702928870293, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI109715829, Length=241, Percent_Identity=26.9709543568465, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI10190704, Length=289, Percent_Identity=25.6055363321799, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI66933014, Length=174, Percent_Identity=26.4367816091954, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI33667109, Length=193, Percent_Identity=30.5699481865285, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504503, Length=197, Percent_Identity=27.9187817258883, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI150247226, Length=208, Percent_Identity=27.4038461538462, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI40548397, Length=208, Percent_Identity=25, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI214010158, Length=208, Percent_Identity=25, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI214010156, Length=208, Percent_Identity=25, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI22758144, Length=208, Percent_Identity=25, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787335, Length=251, Percent_Identity=27.0916334661355, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI87082100, Length=256, Percent_Identity=29.6875, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788459, Length=200, Percent_Identity=33.5, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI2367365, Length=246, Percent_Identity=31.7073170731707, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI2367175, Length=256, Percent_Identity=29.296875, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786812, Length=252, Percent_Identity=28.1746031746032, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787905, Length=248, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=25.6, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789378, Length=149, Percent_Identity=31.5436241610738, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI87082160, Length=262, Percent_Identity=26.7175572519084, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787820, Length=231, Percent_Identity=27.7056277056277, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789208, Length=179, Percent_Identity=29.0502793296089, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=251, Percent_Identity=31.8725099601594, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17555706, Length=248, Percent_Identity=29.0322580645161, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=32.4427480916031, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=33.0827067669173, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=259, Percent_Identity=30.1158301158301, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=30.1526717557252, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=261, Percent_Identity=30.2681992337165, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=257, Percent_Identity=29.1828793774319, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17562990, Length=256, Percent_Identity=31.640625, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17562908, Length=263, Percent_Identity=31.5589353612167, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=258, Percent_Identity=30.2325581395349, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72000259, Length=258, Percent_Identity=29.8449612403101, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=270, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=260, Percent_Identity=29.2307692307692, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17563726, Length=257, Percent_Identity=29.1828793774319, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=27.0161290322581, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=258, Percent_Identity=28.6821705426357, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=260, Percent_Identity=29.6153846153846, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=28.1368821292776, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=269, Percent_Identity=28.2527881040892, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=263, Percent_Identity=29.6577946768061, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=29.9242424242424, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115534694, Length=251, Percent_Identity=27.8884462151394, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538486, Length=262, Percent_Identity=25.5725190839695, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=258, Percent_Identity=28.6821705426357, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=266, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=208, Percent_Identity=27.4038461538462, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322067, Length=196, Percent_Identity=33.6734693877551, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324126, Length=142, Percent_Identity=30.2816901408451, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323882, Length=204, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355319, Length=249, Percent_Identity=31.3253012048193, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643142, Length=256, Percent_Identity=37.109375, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24639444, Length=248, Percent_Identity=31.0483870967742, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI23397609, Length=251, Percent_Identity=30.6772908366534, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24644337, Length=200, Percent_Identity=35, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=30.078125, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571526, Length=263, Percent_Identity=31.1787072243346, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644339, Length=260, Percent_Identity=29.6153846153846, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21358495, Length=204, Percent_Identity=28.4313725490196, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737361, Length=266, Percent_Identity=28.9473684210526, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24656154, Length=278, Percent_Identity=26.6187050359712, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19922628, Length=278, Percent_Identity=26.6187050359712, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 28608; Mature: 28608

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAVQTRRCTAASSLPKVRTMVAVSNQPPRIMLLTGASRGIGHATVKRFSSAGWRVITC
CCCCCCCCCHHHHHCCCHHEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
SRQPFPEQCPWAAGPEDHIQVDLGNPDDTLAAVEEIKNRLPNGELHALVNNAGISPKGEG
CCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GSRLGTLDTPHDVWMQVFQVNFFASIMLARGLVSELEKAQGSVVNVTSIAGSRVHPFAGA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
AYATSKAALAALTREMAADFGPRGVRVNAIAPGEIDTAILSPGTDKIVAQIPLRRLGTPD
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCHHHEECCHHHCCCHH
EVAKIIYVLCTETSSYLNGAEILINGGQHV
HHHHHHHHHCCCCHHHCCCEEEEEECCCCC
>Mature Secondary Structure
MLPAVQTRRCTAASSLPKVRTMVAVSNQPPRIMLLTGASRGIGHATVKRFSSAGWRVITC
CCCCCCCCCHHHHHCCCHHEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEE
SRQPFPEQCPWAAGPEDHIQVDLGNPDDTLAAVEEIKNRLPNGELHALVNNAGISPKGEG
CCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
GSRLGTLDTPHDVWMQVFQVNFFASIMLARGLVSELEKAQGSVVNVTSIAGSRVHPFAGA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
AYATSKAALAALTREMAADFGPRGVRVNAIAPGEIDTAILSPGTDKIVAQIPLRRLGTPD
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEECCCCCHHHEECCHHHCCCHH
EVAKIIYVLCTETSSYLNGAEILINGGQHV
HHHHHHHHHCCCCHHHCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3313281; 11157954; 12597275 [H]