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The map label for this gene is hsrA [H]

Identifier: 158422726

GI number: 158422726

Start: 1229040

End: 1230578

Strand: Direct

Name: hsrA [H]

Synonym: AZC_1102

Alternate gene names: 158422726

Gene position: 1229040-1230578 (Clockwise)

Preceding gene: 158422724

Following gene: 158422728

Centisome position: 22.89

GC content: 68.81

Gene sequence:

>1539_bases
TTGTTGAGTCTGCCGATGCCGTCCCCGCACGTCTCCCAGCCGTCCCCCGAGGCCCCGCCCGCATCCCATGCCCGCAAGGG
CGGCCCCCGCCGCCAGCCGGGCTACCGGGCCGTGGCGCTGATCGTGGCAAGCGCCATGTTCATGGAGCAACTGGACGCGA
CGGTGCTCGCGACCGCCTTGCCGGCGATGGCGCGGGACTTCGGCGTGACGGCGCCGCACATGAGCATCGCGCTCACCTCC
TACCTGCTCTCGCTCGCCGTCTTCATTCCCGCCAGCGGCTGGGCTGCGGACCGGTTCGGCTCGCGCACGGTGTTCCGCCT
CGCCATCGCCGTCTTCACCATCGGCTCGCTTCTGTGCGCGCAGTCCCAGAGCCTCGCCTTCCTCGTCTTCGCCCGCCTGT
TGCAGGGCATGGGCGGGGCGATGATGATCCCGGTCGGCCGCCTCGTGCTGCTGCGCTCCACCACCAAGGCGGACCTCGTC
TCGGCCATGAGCTGGCTGCTGGTGCCGGCGATGATCGGCCCGATGGTCGGTCCGCCGCTCGGCGGCTTCCTCGTCACCTT
CCTCGACTGGCGCTGGATCTTCTACATCAACCTGCCCATCGGCGTGCTGGGGCTGGTGCTGGTCAGCATCTTCATCGCCG
ACAGCCGGGCCGAGAAGCCGGACCGCTTCGATGGCCTCGGCCTCGTTCTCTCGGGTGCGTCGCTGGGCGGCCTGCTGTTC
GCCTTCGAGCTGTCGAGCCGGGGCGAGGGGGATGCCCGCATCACCTTCGGCCTGATGGTGATGGGCGTCCTGAGTGGCCT
TGCGTATCTCCGTCACGCCCGCAAGCTCTCGGCGGCGGGTGGCCATCCGATCCTCGATCTGTCGCTGATGAACATCGAGA
CGTTCCGCCTCTCGGTGATCGCCGGCAACATCACCCGCATCACCCAGGGCGCCCAGCCCTTCCTGATCCCGCTCATGCTC
CAGCTCGGCTTCGGCATGACGGCGGCGGAGGCGGGCATGATCTCGCTCTCCACAGCCGTCGGCTCGCTGCTGATGAAGGG
CGTGGCGCCGAAGGTGCTGCGCCGCTACGGCTTCCGCAACACGTTGCTCGTCAACGGCATCATCTGCACCGGCACCTATG
CGGTCTGCGCCCTCATGCGCCCCGACTGGCCCCACTGGTCCATCTACGTGCTGCTGACCGCCGCCGGCTTCTTCATGTCG
CTCCAGTTCACCGCCTACAATACGGTCGCCTATGACGAGGTGCCGGCGGCGCGCATGAGCGCGGCCGCCAGCTTCTACAG
CACCTTCCAGCAGCTCATGCTCTCCATGGGCATCTGCGTCGCCGGCTTCGCGCTCGCCACCTCCATGGCCGCCAACGGCC
GTTCCGTGGCCGGCTTGGCGGATTTCTCAGTGGCCTTCGTCACCGTCACCACCATCGCCATGGCGGCGACCTTCTGGAAC
GCGCGCCTGCCCCATGATGCGGGTGCCGACATTTCCGGCCACCGCCGCCGGCGGAGCTTGGCTGCCGCCGTCAACGAGGC
GACGGACGCCCCGGACTGA

Upstream 100 bases:

>100_bases
GCGCAATCCGCGCTTTTCGCTGGCGGGAGCGAGGATTAGACGGAATAGATAGCTCCGGCTTCCGCCCTGTTCGCGTCGCG
CCCTGCGCGGCGGTGCGTCG

Downstream 100 bases:

>100_bases
GGGACATCACACCGGCGCCCGGCCGGGGGCGCGAAAAGGCCGGGCGCCGGTTCCGGCCGCGCCGGTCAGGCGACGGCCGG
CTGCCGGCTCCACGAGGGGA

Product: drug resistance translocase

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MLSLPMPSPHVSQPSPEAPPASHARKGGPRRQPGYRAVALIVASAMFMEQLDATVLATALPAMARDFGVTAPHMSIALTS
YLLSLAVFIPASGWAADRFGSRTVFRLAIAVFTIGSLLCAQSQSLAFLVFARLLQGMGGAMMIPVGRLVLLRSTTKADLV
SAMSWLLVPAMIGPMVGPPLGGFLVTFLDWRWIFYINLPIGVLGLVLVSIFIADSRAEKPDRFDGLGLVLSGASLGGLLF
AFELSSRGEGDARITFGLMVMGVLSGLAYLRHARKLSAAGGHPILDLSLMNIETFRLSVIAGNITRITQGAQPFLIPLML
QLGFGMTAAEAGMISLSTAVGSLLMKGVAPKVLRRYGFRNTLLVNGIICTGTYAVCALMRPDWPHWSIYVLLTAAGFFMS
LQFTAYNTVAYDEVPAARMSAAASFYSTFQQLMLSMGICVAGFALATSMAANGRSVAGLADFSVAFVTVTTIAMAATFWN
ARLPHDAGADISGHRRRRSLAAAVNEATDAPD

Sequences:

>Translated_512_residues
MLSLPMPSPHVSQPSPEAPPASHARKGGPRRQPGYRAVALIVASAMFMEQLDATVLATALPAMARDFGVTAPHMSIALTS
YLLSLAVFIPASGWAADRFGSRTVFRLAIAVFTIGSLLCAQSQSLAFLVFARLLQGMGGAMMIPVGRLVLLRSTTKADLV
SAMSWLLVPAMIGPMVGPPLGGFLVTFLDWRWIFYINLPIGVLGLVLVSIFIADSRAEKPDRFDGLGLVLSGASLGGLLF
AFELSSRGEGDARITFGLMVMGVLSGLAYLRHARKLSAAGGHPILDLSLMNIETFRLSVIAGNITRITQGAQPFLIPLML
QLGFGMTAAEAGMISLSTAVGSLLMKGVAPKVLRRYGFRNTLLVNGIICTGTYAVCALMRPDWPHWSIYVLLTAAGFFMS
LQFTAYNTVAYDEVPAARMSAAASFYSTFQQLMLSMGICVAGFALATSMAANGRSVAGLADFSVAFVTVTTIAMAATFWN
ARLPHDAGADISGHRRRRSLAAAVNEATDAPD
>Mature_512_residues
MLSLPMPSPHVSQPSPEAPPASHARKGGPRRQPGYRAVALIVASAMFMEQLDATVLATALPAMARDFGVTAPHMSIALTS
YLLSLAVFIPASGWAADRFGSRTVFRLAIAVFTIGSLLCAQSQSLAFLVFARLLQGMGGAMMIPVGRLVLLRSTTKADLV
SAMSWLLVPAMIGPMVGPPLGGFLVTFLDWRWIFYINLPIGVLGLVLVSIFIADSRAEKPDRFDGLGLVLSGASLGGLLF
AFELSSRGEGDARITFGLMVMGVLSGLAYLRHARKLSAAGGHPILDLSLMNIETFRLSVIAGNITRITQGAQPFLIPLML
QLGFGMTAAEAGMISLSTAVGSLLMKGVAPKVLRRYGFRNTLLVNGIICTGTYAVCALMRPDWPHWSIYVLLTAAGFFMS
LQFTAYNTVAYDEVPAARMSAAASFYSTFQQLMLSMGICVAGFALATSMAANGRSVAGLADFSVAFVTVTTIAMAATFWN
ARLPHDAGADISGHRRRRSLAAAVNEATDAPD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=465, Percent_Identity=37.6344086021505, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1788392, Length=465, Percent_Identity=34.4086021505376, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=26.056338028169, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788710, Length=407, Percent_Identity=26.5356265356265, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI87081983, Length=329, Percent_Identity=27.355623100304, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6325455, Length=148, Percent_Identity=33.1081081081081, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=219, Percent_Identity=28.310502283105, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=30.0653594771242, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=292, Percent_Identity=27.0547945205479, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54350; Mature: 54350

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSLPMPSPHVSQPSPEAPPASHARKGGPRRQPGYRAVALIVASAMFMEQLDATVLATAL
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PAMARDFGVTAPHMSIALTSYLLSLAVFIPASGWAADRFGSRTVFRLAIAVFTIGSLLCA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
QSQSLAFLVFARLLQGMGGAMMIPVGRLVLLRSTTKADLVSAMSWLLVPAMIGPMVGPPL
CCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCH
GGFLVTFLDWRWIFYINLPIGVLGLVLVSIFIADSRAEKPDRFDGLGLVLSGASLGGLLF
HHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEE
AFELSSRGEGDARITFGLMVMGVLSGLAYLRHARKLSAAGGHPILDLSLMNIETFRLSVI
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEE
AGNITRITQGAQPFLIPLMLQLGFGMTAAEAGMISLSTAVGSLLMKGVAPKVLRRYGFRN
ECCHHHHCCCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCC
TLLVNGIICTGTYAVCALMRPDWPHWSIYVLLTAAGFFMSLQFTAYNTVAYDEVPAARMS
EEEECCEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEEECEEECCCCCHHHHH
AAASFYSTFQQLMLSMGICVAGFALATSMAANGRSVAGLADFSVAFVTVTTIAMAATFWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHC
ARLPHDAGADISGHRRRRSLAAAVNEATDAPD
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSLPMPSPHVSQPSPEAPPASHARKGGPRRQPGYRAVALIVASAMFMEQLDATVLATAL
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
PAMARDFGVTAPHMSIALTSYLLSLAVFIPASGWAADRFGSRTVFRLAIAVFTIGSLLCA
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
QSQSLAFLVFARLLQGMGGAMMIPVGRLVLLRSTTKADLVSAMSWLLVPAMIGPMVGPPL
CCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCH
GGFLVTFLDWRWIFYINLPIGVLGLVLVSIFIADSRAEKPDRFDGLGLVLSGASLGGLLF
HHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCEEE
AFELSSRGEGDARITFGLMVMGVLSGLAYLRHARKLSAAGGHPILDLSLMNIETFRLSVI
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEEEEE
AGNITRITQGAQPFLIPLMLQLGFGMTAAEAGMISLSTAVGSLLMKGVAPKVLRRYGFRN
ECCHHHHCCCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCC
TLLVNGIICTGTYAVCALMRPDWPHWSIYVLLTAAGFFMSLQFTAYNTVAYDEVPAARMS
EEEECCEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEEECEEECCCCCHHHHH
AAASFYSTFQQLMLSMGICVAGFALATSMAANGRSVAGLADFSVAFVTVTTIAMAATFWN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHC
ARLPHDAGADISGHRRRRSLAAAVNEATDAPD
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]