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The map label for this gene is nifN [H]

Identifier: 158422661

GI number: 158422661

Start: 1163424

End: 1164791

Strand: Reverse

Name: nifN [H]

Synonym: AZC_1037

Alternate gene names: 158422661

Gene position: 1164791-1163424 (Counterclockwise)

Preceding gene: 158422662

Following gene: 158422660

Centisome position: 21.69

GC content: 66.08

Gene sequence:

>1368_bases
ATGGCCACCGTCGTCCACTCCAGGAAGGCCTGCGTCGTCAATCCCCTGAAGATGAGCCAGCCGATCGGTGGCGCGCTCGC
CTTCATGGGTTTGCGCGGGGCCATGCCGCTGCTGCACGGTTCGCAGGGCTGCACCTCCTTCGGCCTAGTGCTGTTCGTGC
GGCACTTCAAGGAGGCCATCCCCATGCAGACCACCGCCATGAGCGAGGTGGCCACGGTTCTCGGCGGCTACGAGAATGTG
GAACAGGCGGTGCTCAACATCCACAAGCGCACCAAGCCGGAGATCATCGGCATCTGTTCCACGGGCGTCACCGAGACCAA
GGGCGACGACGTGGAGGGCTATCTCAAGCTCATCCGCGAGAAGCATCCGGAGGTCGCCGACCTGCCCATGATCTATGTCT
CCACCCCGGACTTCAAGGACGCCTTCCAGGATGGCTGGGAGAAAACCGTCACCCGCATGGTCGAGGTGCTGGTGGAGGTA
CCGGATCCGGCCAAGCGCAACGCCAAGCGGGTGAATGTGTTGCCGGGCAGCCACCTCACGCCCGGCGACCTTGATGAGCT
GCGCACCATCATCGAGGACTTCGGCCTCATCCCCTCCTTCCTGCCGGATCTCGCCGGCTCGCTGGACGGGCATATTCCCG
ACGAATTCACCCCCACCACCATCGGCGGCATCGGCGTGGACGAGATCGCCGCCATGGGCGAGGCGGGCTGGACGATTGCC
ATCGGCGCGCAGATGCGCCGGGCGGCCGAGGCCATGGAGAAGAAGACCGGCGTGCCCTTCCGCCTGTTCGAGCGGCTGTG
CGGGCTGGAGGCGAATGATGAATTCATCATGTTCCTCAGCCAGATCTCCGGCCGCCCGGTGCCGCGCAAGTATCGCCGCC
AGCGCGGCCAGCTGGTGGATGCCATGCTGGACGCCCATTTCCACATCGGCGGCCGCCGGCTTGCCATCGGCGCCGAACCG
GACCTCCTGTTCGACATGTCGTCCGTGCTGCACGAGATGGGGGCGCATGTGACCGCCGCCGTCACCACCACCCATTCGCC
GGTGCTGGAGCGGGTCCACACCGACGAGGTGCTGATCGGCGATCTGGAGGATCTGGAGCGCCTCGCCAAGGAGCGCGAGT
GCGATCTGCTCATCACCCATTCGCACGGGCGGCAGGCGGCCTCCCGGCTCAACATTCCCTTCTACCGGGCGGGCATTCCC
ATGTTCGACCGGCTGGGCGCCGGCCACCAGCTCTCCGTGGGCTATCGCGGCACCCGCACCACGCTCTTCACCATCGCCAA
CCTTCTCATCAGTGACCACGAGGCCAACCACGACGCCACGCCCGACACCTGGCGGACGGACGGCACTCATGCCGCTCCCA
TGCATTGA

Upstream 100 bases:

>100_bases
GCGGCTGCGGCGCCTGCGCCATCCGCATCGAGGAGATCCTGGCCGAGCCCCTGCCCGCGGTGGCAGCGGCGCCGGCGCTG
CGGGCGGCGGAGTGAGCGCC

Downstream 100 bases:

>100_bases
TCGGCCAACGCAAGGACGCACGCCCATGAAGATCGCCTTCGCGACCCAGGATTTGAAGCGCGTGGATGCCCATTTCGGCT
GGGCCAAGAACATCGCCATC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MATVVHSRKACVVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAIPMQTTAMSEVATVLGGYENV
EQAVLNIHKRTKPEIIGICSTGVTETKGDDVEGYLKLIREKHPEVADLPMIYVSTPDFKDAFQDGWEKTVTRMVEVLVEV
PDPAKRNAKRVNVLPGSHLTPGDLDELRTIIEDFGLIPSFLPDLAGSLDGHIPDEFTPTTIGGIGVDEIAAMGEAGWTIA
IGAQMRRAAEAMEKKTGVPFRLFERLCGLEANDEFIMFLSQISGRPVPRKYRRQRGQLVDAMLDAHFHIGGRRLAIGAEP
DLLFDMSSVLHEMGAHVTAAVTTTHSPVLERVHTDEVLIGDLEDLERLAKERECDLLITHSHGRQAASRLNIPFYRAGIP
MFDRLGAGHQLSVGYRGTRTTLFTIANLLISDHEANHDATPDTWRTDGTHAAPMH

Sequences:

>Translated_455_residues
MATVVHSRKACVVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAIPMQTTAMSEVATVLGGYENV
EQAVLNIHKRTKPEIIGICSTGVTETKGDDVEGYLKLIREKHPEVADLPMIYVSTPDFKDAFQDGWEKTVTRMVEVLVEV
PDPAKRNAKRVNVLPGSHLTPGDLDELRTIIEDFGLIPSFLPDLAGSLDGHIPDEFTPTTIGGIGVDEIAAMGEAGWTIA
IGAQMRRAAEAMEKKTGVPFRLFERLCGLEANDEFIMFLSQISGRPVPRKYRRQRGQLVDAMLDAHFHIGGRRLAIGAEP
DLLFDMSSVLHEMGAHVTAAVTTTHSPVLERVHTDEVLIGDLEDLERLAKERECDLLITHSHGRQAASRLNIPFYRAGIP
MFDRLGAGHQLSVGYRGTRTTLFTIANLLISDHEANHDATPDTWRTDGTHAAPMH
>Mature_454_residues
ATVVHSRKACVVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAIPMQTTAMSEVATVLGGYENVE
QAVLNIHKRTKPEIIGICSTGVTETKGDDVEGYLKLIREKHPEVADLPMIYVSTPDFKDAFQDGWEKTVTRMVEVLVEVP
DPAKRNAKRVNVLPGSHLTPGDLDELRTIIEDFGLIPSFLPDLAGSLDGHIPDEFTPTTIGGIGVDEIAAMGEAGWTIAI
GAQMRRAAEAMEKKTGVPFRLFERLCGLEANDEFIMFLSQISGRPVPRKYRRQRGQLVDAMLDAHFHIGGRRLAIGAEPD
LLFDMSSVLHEMGAHVTAAVTTTHSPVLERVHTDEVLIGDLEDLERLAKERECDLLITHSHGRQAASRLNIPFYRAGIPM
FDRLGAGHQLSVGYRGTRTTLFTIANLLISDHEANHDATPDTWRTDGTHAAPMH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 49900; Mature: 49769

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVVHSRKACVVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAI
CCCCCCCCCCCEECCHHHCCCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHC
PMQTTAMSEVATVLGGYENVEQAVLNIHKRTKPEIIGICSTGVTETKGDDVEGYLKLIRE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KHPEVADLPMIYVSTPDFKDAFQDGWEKTVTRMVEVLVEVPDPAKRNAKRVNVLPGSHLT
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCCCC
PGDLDELRTIIEDFGLIPSFLPDLAGSLDGHIPDEFTPTTIGGIGVDEIAAMGEAGWTIA
CCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEE
IGAQMRRAAEAMEKKTGVPFRLFERLCGLEANDEFIMFLSQISGRPVPRKYRRQRGQLVD
ECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
AMLDAHFHIGGRRLAIGAEPDLLFDMSSVLHEMGAHVTAAVTTTHSPVLERVHTDEVLIG
HHHHHHHEECCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEC
DLEDLERLAKERECDLLITHSHGRQAASRLNIPFYRAGIPMFDRLGAGHQLSVGYRGTRT
CHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEECCCCHHHHCCCCCEEEECCCCCHH
TLFTIANLLISDHEANHDATPDTWRTDGTHAAPMH
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATVVHSRKACVVNPLKMSQPIGGALAFMGLRGAMPLLHGSQGCTSFGLVLFVRHFKEAI
CCCCCCCCCCEECCHHHCCCCCHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHC
PMQTTAMSEVATVLGGYENVEQAVLNIHKRTKPEIIGICSTGVTETKGDDVEGYLKLIRE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KHPEVADLPMIYVSTPDFKDAFQDGWEKTVTRMVEVLVEVPDPAKRNAKRVNVLPGSHLT
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEECCCCCCC
PGDLDELRTIIEDFGLIPSFLPDLAGSLDGHIPDEFTPTTIGGIGVDEIAAMGEAGWTIA
CCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEE
IGAQMRRAAEAMEKKTGVPFRLFERLCGLEANDEFIMFLSQISGRPVPRKYRRQRGQLVD
ECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
AMLDAHFHIGGRRLAIGAEPDLLFDMSSVLHEMGAHVTAAVTTTHSPVLERVHTDEVLIG
HHHHHHHEECCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEC
DLEDLERLAKERECDLLITHSHGRQAASRLNIPFYRAGIPMFDRLGAGHQLSVGYRGTRT
CHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCEECCCCHHHHCCCCCEEEECCCCCHH
TLFTIANLLISDHEANHDATPDTWRTDGTHAAPMH
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]