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The map label for this gene is nifE [H]

Identifier: 158422662

GI number: 158422662

Start: 1164797

End: 1166479

Strand: Reverse

Name: nifE [H]

Synonym: AZC_1038

Alternate gene names: 158422662

Gene position: 1166479-1164797 (Counterclockwise)

Preceding gene: 158422663

Following gene: 158422661

Centisome position: 21.72

GC content: 66.07

Gene sequence:

>1683_bases
ATGACGTCGCTCTCGGCCACCATTCAGGACGTGTTCAACGAGCCCGGCTGCGGCAAGAACGCCAACAAGTCCGAGGCCGA
GCGCAAGAAGGGCTGCACCAAGCAGCTCCAGCCCGGCGGCGCCGCCGGCGGCTGCGCCTTCGATGGCGCGAAGATAGCCC
TCCAGCCGCTTACCGACGTCGCTCACCTCGTGCACGGCCCCATCGCCTGCGAGGGTAACAGCTGGGACAACCGCGGCGCC
CGCTCCTCCGGCTCGGACCTCTGGCGCACCAGCTTCACCACCGACATGAACGAGACGGACGTGGTGTTCGGCGGCGAGAA
GCGCCTGTTCAAGGCGATCCGCGAGACCATCGAGAAATATGATCCGCCCGCCGTCTTCGTCTACCAGACCTGCGTGCCGG
CCATGACCGGCGACGACATCAACGCGGTCTGCAAGGCGGCGAAGGAGAAGTTCGGCAAGCCGGTGATCCCGATCAATTCG
CCCGGCTTCGTCGGCCCCAAGAACCTCGGCAACAAGCTCGCGGGCGAAGCCCTGCTGGAGCATGTGATCGGCACCGAGGA
ACCTGAATATACGACGCCCTACGACCTCAACATCATCGGCGAATACAATCTCGCGGGCGAGCTGTGGCAGATCAAGCCGC
TGTTCGACGAGCTCGGCATCCGCATCTTCGCCTCCATCTCCGGCGACGGGCGCTACAAGGAAGTGGCCTATTCCCACCGC
GCCAAGGCGGCGATGATGGTGTGCTCCAAGGCCATGATCAATGTCGCCCGCAAGATGGAGGAGCGCTACGGCATCCCGTT
CTTCGAGGGCTCCTTCTACGGCATCGAGGATTCGAGCGACTCCCTGCGGGAGATCGCGCGCCTGCTCATCGAGCGCGGCG
CCCCGGCGGAACTGATGGAGCGCACCGAGGCGGTGATCGCCCGCGAGGAGGCCAAGGCCTTCGCGGCGATCGCCGCCTAC
AAGCCCCGTTTCCAGGGCAAGAAGGTGCTGCTCATCACCGGCGGCGTGAAGTCCTGGTCGGTGGTCGCGGCTCTTCAGGA
GGCGGGCCTGGAGCTGGTGGGCACTTCGGTGAAGAAGTCCACCCGCGAGGACAAGGAGCGCATCAAGGAGCTGATGGGTC
AGGATGCCCACATGATCGAGGACATGACGCCTCGTGAAATGTACAAGATGCTCAAGGATGCGAAGGCCGATATCATGCTT
TCGGGCGGCCGCTCGCAATTCATCGCGCTGAAGGCCGCGATGCCCTGGCTCGACATCAACCAGGAACGCCACCACGCCTA
TATGGGCTATGTGGGCATGGTGAAGCTGGTGGAGGAGATCGACAAGGCGCTCTTCAACCCCATGTGGGAACAGGTGCGCA
AGCCCGCCCCGTGGGATCGCCCCGGCGAGAGCTGGCAGGCCAAGGCGCTGGCCCAGATGGATGCGGAAGCCGCCGTGCTC
GCCGCCGATCCGGTGGCCGCCGAGGCCGCCCGGCGTGCCAAGAAAGTGTGCCAGTGCAAGAGCGTGGACGTCGGGACCAT
CGAGGATGCCATCCGCGCCCACGGCCTTTCCACCGTGGAGGGCGTGCGCACACACACCAACGCCTCCGGCGGCTGCGGCG
CCTGCGCCATCCGCATCGAGGAGATCCTGGCCGAGCCCCTGCCCGCGGTGGCAGCGGCGCCGGCGCTGCGGGCGGCGGAG
TGA

Upstream 100 bases:

>100_bases
GCGGGGCCTTCGCGCGTTTCGCGCCCCTTATCTCCGATCCTCTGGCGTGACGCCGCCCGGCGCCCACCGTTCCGCCCACG
ATGACTTCCGGAGAAGCCCC

Downstream 100 bases:

>100_bases
GCGCCATGGCCACCGTCGTCCACTCCAGGAAGGCCTGCGTCGTCAATCCCCTGAAGATGAGCCAGCCGATCGGTGGCGCG
CTCGCCTTCATGGGTTTGCG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTSLSATIQDVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDVAHLVHGPIACEGNSWDNRGA
RSSGSDLWRTSFTTDMNETDVVFGGEKRLFKAIRETIEKYDPPAVFVYQTCVPAMTGDDINAVCKAAKEKFGKPVIPINS
PGFVGPKNLGNKLAGEALLEHVIGTEEPEYTTPYDLNIIGEYNLAGELWQIKPLFDELGIRIFASISGDGRYKEVAYSHR
AKAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIEDSSDSLREIARLLIERGAPAELMERTEAVIAREEAKAFAAIAAY
KPRFQGKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKSTREDKERIKELMGQDAHMIEDMTPREMYKMLKDAKADIML
SGGRSQFIALKAAMPWLDINQERHHAYMGYVGMVKLVEEIDKALFNPMWEQVRKPAPWDRPGESWQAKALAQMDAEAAVL
AADPVAAEAARRAKKVCQCKSVDVGTIEDAIRAHGLSTVEGVRTHTNASGGCGACAIRIEEILAEPLPAVAAAPALRAAE

Sequences:

>Translated_560_residues
MTSLSATIQDVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDVAHLVHGPIACEGNSWDNRGA
RSSGSDLWRTSFTTDMNETDVVFGGEKRLFKAIRETIEKYDPPAVFVYQTCVPAMTGDDINAVCKAAKEKFGKPVIPINS
PGFVGPKNLGNKLAGEALLEHVIGTEEPEYTTPYDLNIIGEYNLAGELWQIKPLFDELGIRIFASISGDGRYKEVAYSHR
AKAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIEDSSDSLREIARLLIERGAPAELMERTEAVIAREEAKAFAAIAAY
KPRFQGKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKSTREDKERIKELMGQDAHMIEDMTPREMYKMLKDAKADIML
SGGRSQFIALKAAMPWLDINQERHHAYMGYVGMVKLVEEIDKALFNPMWEQVRKPAPWDRPGESWQAKALAQMDAEAAVL
AADPVAAEAARRAKKVCQCKSVDVGTIEDAIRAHGLSTVEGVRTHTNASGGCGACAIRIEEILAEPLPAVAAAPALRAAE
>Mature_559_residues
TSLSATIQDVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDVAHLVHGPIACEGNSWDNRGAR
SSGSDLWRTSFTTDMNETDVVFGGEKRLFKAIRETIEKYDPPAVFVYQTCVPAMTGDDINAVCKAAKEKFGKPVIPINSP
GFVGPKNLGNKLAGEALLEHVIGTEEPEYTTPYDLNIIGEYNLAGELWQIKPLFDELGIRIFASISGDGRYKEVAYSHRA
KAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIEDSSDSLREIARLLIERGAPAELMERTEAVIAREEAKAFAAIAAYK
PRFQGKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKSTREDKERIKELMGQDAHMIEDMTPREMYKMLKDAKADIMLS
GGRSQFIALKAAMPWLDINQERHHAYMGYVGMVKLVEEIDKALFNPMWEQVRKPAPWDRPGESWQAKALAQMDAEAAVLA
ADPVAAEAARRAKKVCQCKSVDVGTIEDAIRAHGLSTVEGVRTHTNASGGCGACAIRIEEILAEPLPAVAAAPALRAAE

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 60887; Mature: 60756

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSATIQDVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDV
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEHHHHHH
AHLVHGPIACEGNSWDNRGARSSGSDLWRTSFTTDMNETDVVFGGEKRLFKAIRETIEKY
HHHHCCCEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
DPPAVFVYQTCVPAMTGDDINAVCKAAKEKFGKPVIPINSPGFVGPKNLGNKLAGEALLE
CCCEEEEEEHHCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
HVIGTEEPEYTTPYDLNIIGEYNLAGELWQIKPLFDELGIRIFASISGDGRYKEVAYSHR
HHHCCCCCCCCCCCCEEEEECCCCCCCCEEECHHHHHCCEEEEEEECCCCCHHHHHHHHH
AKAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIEDSSDSLREIARLLIERGAPAELME
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCCHHHHH
RTEAVIAREEAKAFAAIAAYKPRFQGKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
TREDKERIKELMGQDAHMIEDMTPREMYKMLKDAKADIMLSGGRSQFIALKAAMPWLDIN
HHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCC
QERHHAYMGYVGMVKLVEEIDKALFNPMWEQVRKPAPWDRPGESWQAKALAQMDAEAAVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEE
AADPVAAEAARRAKKVCQCKSVDVGTIEDAIRAHGLSTVEGVRTHTNASGGCGACAIRIE
ECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHH
EILAEPLPAVAAAPALRAAE
HHHHCCCCHHHHCCCHHCCC
>Mature Secondary Structure 
TSLSATIQDVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKIALQPLTDV
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEHHHHHH
AHLVHGPIACEGNSWDNRGARSSGSDLWRTSFTTDMNETDVVFGGEKRLFKAIRETIEKY
HHHHCCCEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
DPPAVFVYQTCVPAMTGDDINAVCKAAKEKFGKPVIPINSPGFVGPKNLGNKLAGEALLE
CCCEEEEEEHHCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
HVIGTEEPEYTTPYDLNIIGEYNLAGELWQIKPLFDELGIRIFASISGDGRYKEVAYSHR
HHHCCCCCCCCCCCCEEEEECCCCCCCCEEECHHHHHCCEEEEEEECCCCCHHHHHHHHH
AKAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIEDSSDSLREIARLLIERGAPAELME
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCCHHHHH
RTEAVIAREEAKAFAAIAAYKPRFQGKKVLLITGGVKSWSVVAALQEAGLELVGTSVKKS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
TREDKERIKELMGQDAHMIEDMTPREMYKMLKDAKADIMLSGGRSQFIALKAAMPWLDIN
HHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCC
QERHHAYMGYVGMVKLVEEIDKALFNPMWEQVRKPAPWDRPGESWQAKALAQMDAEAAVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCEEE
AADPVAAEAARRAKKVCQCKSVDVGTIEDAIRAHGLSTVEGVRTHTNASGGCGACAIRIE
ECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHH
EILAEPLPAVAAAPALRAAE
HHHHCCCCHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]