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The map label for this gene is proP [H]

Identifier: 158422655

GI number: 158422655

Start: 1157544

End: 1158809

Strand: Direct

Name: proP [H]

Synonym: AZC_1031

Alternate gene names: 158422655

Gene position: 1157544-1158809 (Clockwise)

Preceding gene: 158422654

Following gene: 158422656

Centisome position: 21.56

GC content: 68.88

Gene sequence:

>1266_bases
GTGTCCGTCGCGATCGTCAGTGAGCGCCCCTCGGCCCGCCTCGTTGCCGGTGGCGCCATCGGCAACATGCTGGAGTGGTA
CGATTTCGGCGTGTACGGCTTTCTGGCCTCGGTGTTCGCGCGCAACTTCTTTCCCGACAGCAGCGCCTTCATCGGCCTCC
TGTCGGCCTTCGGCATGTTCGCCGCCTCCTTCCTGATGCGGCCGCTGGGTGGCATCCTCTTTGGCCGGGTGGGTGACCGG
CTGGGGCGGCGGCGGGCGCTGTTCCTCTCCGCCATGATGATGACCCTCTCCACGGTGAGCATCGGCCTGCTGCCGACCGC
CGCCAGCGCGGGGGCGATGGCCCCTGTCCTCTTGCTGGCGCTGCGCCTGTTGCAGGGCATTTCCGTGGGCGGCGAGCAGG
CGGCGGCGGTGGTGTTTCTGTCCGAGGCGGCGCCACCCCGGCGGCGCGGGCTGCTCGCCTCCCTTTCCGTGGTGGGGGCG
ACGCTCGGAACCCTGCTGGGCTCGGCCGTGGGCGCCTCGGTCGCCAGCCTGCTGCCCCCGCAGGACATGGCCGACTGGGG
TTGGCGCGTGCCCTTCCTGCTGGGATTGGTGCTGGGCGGGGCGGCGCTGATCCTGCGCGCCGGCAAGGGCGAGCCCGAGC
CAGATCGGACTGCCACGCCCAAGGCCCCCTTGCGGGAGGCTATGCGCGCGCATGGCCGCCAGATCCTCCACGCCATGCTG
ATCGTGGTGGCGGAAGGCAATGTCTTCTATCTCGCCTTTGTCTTCCTCGCGACCTACATGCAGAAGGTGGACGGGCTGCC
GCTCGCCAAATCCCTGCAGATGAGCGTGTTCGGCATGGTGGTGGTGCTCATCGCCAGCCCGGCGGCGGCCCTGCTGTCGG
ACCGGATCGGCCGCAAGAAGGTGCTTCTGGCCGCGCTCGTCGGCCTCATCCTGTGTGCCTGGCCAGTGTTCCGATTGCTG
AGTTCGGACAACCTGCCGCAGATGATCGCCGGGCAGGTCATCCTCGCCCTTCTCGCCTCCTGCTACGGCGGCCCCATGCC
GGCGGCGCTGTGCGAACTCTTCCAACGCCGCACCCGCTGCACCGCATATGCCATCGCCTGGAATTGCGGTGTCGGCTGGG
TCGGCGGGCTCACGCCGATGCTGACCATCTATCTCATGCACCGCTTCGACAGCCCCATGGCGCCGGCGGTGATGGTGATC
GTCCTCTCCTGCGTCTCGCTGGCCGGCGTCCTTGCCATGACGGACAAATCGGTCGGCCCGATCTGA

Upstream 100 bases:

>100_bases
TCGCCACGTTGTGCATGACCGACCGGACACGGCTCCCGCTGCCATGAGGTGGGACAAGGCTTCCGCCGATTTTGCGGATG
ATGGATCCGGAGAGGGGGCC

Downstream 100 bases:

>100_bases
TCCCTCCGCAGGGCGGCCGGGGACAAGATGGGCCGCCCCGTCTCGACGCGGCGGCCGAAAAGCTCTAGCACACGGGCAGA
CATTCGATCCTCACGAACCG

Product: proline/betaine transporter 2

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSVAIVSERPSARLVAGGAIGNMLEWYDFGVYGFLASVFARNFFPDSSAFIGLLSAFGMFAASFLMRPLGGILFGRVGDR
LGRRRALFLSAMMMTLSTVSIGLLPTAASAGAMAPVLLLALRLLQGISVGGEQAAAVVFLSEAAPPRRRGLLASLSVVGA
TLGTLLGSAVGASVASLLPPQDMADWGWRVPFLLGLVLGGAALILRAGKGEPEPDRTATPKAPLREAMRAHGRQILHAML
IVVAEGNVFYLAFVFLATYMQKVDGLPLAKSLQMSVFGMVVVLIASPAAALLSDRIGRKKVLLAALVGLILCAWPVFRLL
SSDNLPQMIAGQVILALLASCYGGPMPAALCELFQRRTRCTAYAIAWNCGVGWVGGLTPMLTIYLMHRFDSPMAPAVMVI
VLSCVSLAGVLAMTDKSVGPI

Sequences:

>Translated_421_residues
MSVAIVSERPSARLVAGGAIGNMLEWYDFGVYGFLASVFARNFFPDSSAFIGLLSAFGMFAASFLMRPLGGILFGRVGDR
LGRRRALFLSAMMMTLSTVSIGLLPTAASAGAMAPVLLLALRLLQGISVGGEQAAAVVFLSEAAPPRRRGLLASLSVVGA
TLGTLLGSAVGASVASLLPPQDMADWGWRVPFLLGLVLGGAALILRAGKGEPEPDRTATPKAPLREAMRAHGRQILHAML
IVVAEGNVFYLAFVFLATYMQKVDGLPLAKSLQMSVFGMVVVLIASPAAALLSDRIGRKKVLLAALVGLILCAWPVFRLL
SSDNLPQMIAGQVILALLASCYGGPMPAALCELFQRRTRCTAYAIAWNCGVGWVGGLTPMLTIYLMHRFDSPMAPAVMVI
VLSCVSLAGVLAMTDKSVGPI
>Mature_420_residues
SVAIVSERPSARLVAGGAIGNMLEWYDFGVYGFLASVFARNFFPDSSAFIGLLSAFGMFAASFLMRPLGGILFGRVGDRL
GRRRALFLSAMMMTLSTVSIGLLPTAASAGAMAPVLLLALRLLQGISVGGEQAAAVVFLSEAAPPRRRGLLASLSVVGAT
LGTLLGSAVGASVASLLPPQDMADWGWRVPFLLGLVLGGAALILRAGKGEPEPDRTATPKAPLREAMRAHGRQILHAMLI
VVAEGNVFYLAFVFLATYMQKVDGLPLAKSLQMSVFGMVVVLIASPAAALLSDRIGRKKVLLAALVGLILCAWPVFRLLS
SDNLPQMIAGQVILALLASCYGGPMPAALCELFQRRTRCTAYAIAWNCGVGWVGGLTPMLTIYLMHRFDSPMAPAVMVIV
LSCVSLAGVLAMTDKSVGPI

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=434, Percent_Identity=32.0276497695853, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1788292, Length=439, Percent_Identity=30.5239179954442, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1788942, Length=352, Percent_Identity=32.9545454545455, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789941, Length=368, Percent_Identity=32.3369565217391, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI87082231, Length=249, Percent_Identity=24.0963855421687, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082404, Length=336, Percent_Identity=23.2142857142857, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 44252; Mature: 44120

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAIVSERPSARLVAGGAIGNMLEWYDFGVYGFLASVFARNFFPDSSAFIGLLSAFGMF
CCEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AASFLMRPLGGILFGRVGDRLGRRRALFLSAMMMTLSTVSIGLLPTAASAGAMAPVLLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LRLLQGISVGGEQAAAVVFLSEAAPPRRRGLLASLSVVGATLGTLLGSAVGASVASLLPP
HHHHHCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QDMADWGWRVPFLLGLVLGGAALILRAGKGEPEPDRTATPKAPLREAMRAHGRQILHAML
CCHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IVVAEGNVFYLAFVFLATYMQKVDGLPLAKSLQMSVFGMVVVLIASPAAALLSDRIGRKK
HHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHH
VLLAALVGLILCAWPVFRLLSSDNLPQMIAGQVILALLASCYGGPMPAALCELFQRRTRC
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TAYAIAWNCGVGWVGGLTPMLTIYLMHRFDSPMAPAVMVIVLSCVSLAGVLAMTDKSVGP
HHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
I
C
>Mature Secondary Structure 
SVAIVSERPSARLVAGGAIGNMLEWYDFGVYGFLASVFARNFFPDSSAFIGLLSAFGMF
CEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AASFLMRPLGGILFGRVGDRLGRRRALFLSAMMMTLSTVSIGLLPTAASAGAMAPVLLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LRLLQGISVGGEQAAAVVFLSEAAPPRRRGLLASLSVVGATLGTLLGSAVGASVASLLPP
HHHHHCCCCCCCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QDMADWGWRVPFLLGLVLGGAALILRAGKGEPEPDRTATPKAPLREAMRAHGRQILHAML
CCHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IVVAEGNVFYLAFVFLATYMQKVDGLPLAKSLQMSVFGMVVVLIASPAAALLSDRIGRKK
HHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHH
VLLAALVGLILCAWPVFRLLSSDNLPQMIAGQVILALLASCYGGPMPAALCELFQRRTRC
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TAYAIAWNCGVGWVGGLTPMLTIYLMHRFDSPMAPAVMVIVLSCVSLAGVLAMTDKSVGP
HHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]