Click here to switch to the map view.

The map label for this gene is glyS [H]

Identifier: 158422656

GI number: 158422656

Start: 1158932

End: 1161166

Strand: Direct

Name: glyS [H]

Synonym: AZC_1032

Alternate gene names: 158422656

Gene position: 1158932-1161166 (Clockwise)

Preceding gene: 158422655

Following gene: 158422657

Centisome position: 21.58

GC content: 68.95

Gene sequence:

>2235_bases
ATGCCCGATCTGCTCCTTGAACTGTTCTCCGAAGAAATCCCCGCCCGCATGCAGGCGCAGGCCGCCGACACGCTGCGCAA
GCTCGTCACCGACGCGCTGGTGGAGCGGGGCCTGCTCTATGAGGGCGCCAAGGCCTTCGTGACGCCGCGCCGCCTCGCGC
TCGCCGTCCACGGCTTGCCCGGCCGGCAGGCGGACAGCCGGGAAGAACGCAAGGGTCCGCGTATGGGCGCGCCGGAAGGC
GCCATCCAGGGCTTCCTGAAGAGCGCGGGCCTTGCCTCCATCGATCAGGCGAAGATCGAGAGCGACCCCAAGAAGGGTGA
GTTCTACGTCGCGGTCATCGAGCGCCCCGGCAAGGCGACCACCGAGGTGATTGCCGAGATCGTGCCCGAGATCGTGCGCA
CCTTTCCGTGGCCGAAGTCCATGCGCTGGGGCACCGGCACCATGCGCTGGGTGCGGCCGCTGCATTCCATCGTCTGCACC
TTCGGCACCGAGGTGGAAGACCCCGAGGTGGTGCGCTTCGAGGTGGAGGGTATCGTCTCCGGCGACGTGACCCGTGGCCA
CCGCTTCCTCGCCCCCGAGCCCTTCGCGGTGCGGCGGCTCGAGGACTGGATGGCCAAGCTTGAGGCGGCGAAGGTTGTCG
CCGACCGCGACCGCCGCAAGGCCATCATCCTCAACGACGCCAAGGATCTTGCCCTCGTGCACGGGCTGGAGCTGGTGGAG
GACGAGGGCCTGCTGGAGGAGGTGTGCGGCCTCGTGGAATGGCCCGTCACCCTCATGGGCAGCTTCGAGGAGCGCTTCCT
CGACATTCCCGACGAGGTGATCCGCGCCACCATCCGCGCCAACCAGAAGTGCTTCGTGCTGCGCGATCCGCGCACGGGGC
GCCTCGCCAACCGCTTCATCCTCGTCTCCAACATTGCCGCCACCGACGGCGGTGCGGCCATCGTGGCAGGCAACGAGCGC
GTGATCCGCGCCCGCCTGTCGGATGCCAAATTCTTCTGGGATACCGACCGCCGCACCCCCATGGCGGACCGGATGTCCAA
GTTCGACGCCATCACCTTCCACGAGAAGCTCGGCTCTCAGGGCGCGCGCATCAACCGCATCGTGGCGCTGGCCCGCGAGA
TCGCGCCCCGCGTCGGCGCCGACGTGGACCTGACCGAACAGGCCGCGCGTCTCGCCAAGGCGGACCTGCTCACGGAAGTG
GTGGGCGAGTTCCCCGAGTTGCAGGGCCTCATGGGCCGCTATTATGCCCAGCACGAGAAGCTGCCGCCGGAAGTCGCAGC
CGCCTGCGAGGAGCATTACAAGCCGCAGGGGCCGTCCGACCGCGTGCCCACCGCGCCCGTCTCCATCGCCGTGGCGCTGG
CGGACAAGCTCGACACGCTGGTGGGCTTCTGGGCCATCGACGAGAAGCCGACCGGATCCAAGGACCCCTACGCGCTGCGC
CGTGCTGCGCTGGGCGTCATCCGCATCATTCTCGACAACAATCTGCGCCTGCCGCTGCGCCCGCTGTTGGTGAGCGCCTC
GCGCACCATCCTCGGCGAGGAGCATTTCGAGCGCCACCCGCACGATGTGTTCCGTCTGGAGACCATCGCCGACAAGGTGG
TGAGCGATCTCCTGCAGGGGCTCGCCCGCCGCCTCGCCAACCTGCCCGCGGATGCGGACGTGGAGAGCGCCGAGCGCGTG
GAGGGCATGACGCTGTCCGGTGACCTCGCCGACGACCTGCTGGCCTTCTTCGGCGACCGCCTGAAGGTGCATCTGCGCGA
CCGTGGCGCTCGCCACGACCTCGTGGATGCCGTCTTCGCGCTGCCGGGGCAGGACGACCTGCTGCTGGTGGATCGCCGCG
TGGCGGCCCTCGCGGCCTTCCTGGAGACCGAGGACGGGCGCACGTTGCTCGCTGGCTATCGCCGCGCGGTGAACATCCTG
CGCATCGAGGAGAAGAAGGACGGCGTGGCCTACAAGGGCCCGTCCGAGGCCAAGCTCCTGAAGGAGCCGGCGGAGGAGCA
TCTCGCCGCCGCCATCGAGACCGTGAAGCGGCAGGTGGCGGATGCGGTGGCGCGCGAGGACTTCGCCGCCGCCATGGGCG
CGCTCGCCCATCTGCGGGGTCCGGTGGACGCCTTCTTCGAAGGCGTGACGGTGAACGCGCCGGAGGCCGCGCTGCGCATC
AACCGGCTGAAGCTGCTGGATGAAATCCGCTCTGCTACGCGCGGCATCGCGGATTTCGACCGCATCGAAGGTTGA

Upstream 100 bases:

>100_bases
ACAAGATGGGCCGCCCCGTCTCGACGCGGCGGCCGAAAAGCTCTAGCACACGGGCAGACATTCGATCCTCACGAACCGGG
GCCCAGCGCCGCGCGATCCC

Downstream 100 bases:

>100_bases
GGTAAACGGGGTCTGAATCCGCTCTGTCCCGTTAACGCAAGTCTTTCTTAAGATAGATGAAGAAAACGCCCGCCGGCTGA
TCCCGGCGGGCGTTTTGCGT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MPDLLLELFSEEIPARMQAQAADTLRKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLPGRQADSREERKGPRMGAPEG
AIQGFLKSAGLASIDQAKIESDPKKGEFYVAVIERPGKATTEVIAEIVPEIVRTFPWPKSMRWGTGTMRWVRPLHSIVCT
FGTEVEDPEVVRFEVEGIVSGDVTRGHRFLAPEPFAVRRLEDWMAKLEAAKVVADRDRRKAIILNDAKDLALVHGLELVE
DEGLLEEVCGLVEWPVTLMGSFEERFLDIPDEVIRATIRANQKCFVLRDPRTGRLANRFILVSNIAATDGGAAIVAGNER
VIRARLSDAKFFWDTDRRTPMADRMSKFDAITFHEKLGSQGARINRIVALAREIAPRVGADVDLTEQAARLAKADLLTEV
VGEFPELQGLMGRYYAQHEKLPPEVAAACEEHYKPQGPSDRVPTAPVSIAVALADKLDTLVGFWAIDEKPTGSKDPYALR
RAALGVIRIILDNNLRLPLRPLLVSASRTILGEEHFERHPHDVFRLETIADKVVSDLLQGLARRLANLPADADVESAERV
EGMTLSGDLADDLLAFFGDRLKVHLRDRGARHDLVDAVFALPGQDDLLLVDRRVAALAAFLETEDGRTLLAGYRRAVNIL
RIEEKKDGVAYKGPSEAKLLKEPAEEHLAAAIETVKRQVADAVAREDFAAAMGALAHLRGPVDAFFEGVTVNAPEAALRI
NRLKLLDEIRSATRGIADFDRIEG

Sequences:

>Translated_744_residues
MPDLLLELFSEEIPARMQAQAADTLRKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLPGRQADSREERKGPRMGAPEG
AIQGFLKSAGLASIDQAKIESDPKKGEFYVAVIERPGKATTEVIAEIVPEIVRTFPWPKSMRWGTGTMRWVRPLHSIVCT
FGTEVEDPEVVRFEVEGIVSGDVTRGHRFLAPEPFAVRRLEDWMAKLEAAKVVADRDRRKAIILNDAKDLALVHGLELVE
DEGLLEEVCGLVEWPVTLMGSFEERFLDIPDEVIRATIRANQKCFVLRDPRTGRLANRFILVSNIAATDGGAAIVAGNER
VIRARLSDAKFFWDTDRRTPMADRMSKFDAITFHEKLGSQGARINRIVALAREIAPRVGADVDLTEQAARLAKADLLTEV
VGEFPELQGLMGRYYAQHEKLPPEVAAACEEHYKPQGPSDRVPTAPVSIAVALADKLDTLVGFWAIDEKPTGSKDPYALR
RAALGVIRIILDNNLRLPLRPLLVSASRTILGEEHFERHPHDVFRLETIADKVVSDLLQGLARRLANLPADADVESAERV
EGMTLSGDLADDLLAFFGDRLKVHLRDRGARHDLVDAVFALPGQDDLLLVDRRVAALAAFLETEDGRTLLAGYRRAVNIL
RIEEKKDGVAYKGPSEAKLLKEPAEEHLAAAIETVKRQVADAVAREDFAAAMGALAHLRGPVDAFFEGVTVNAPEAALRI
NRLKLLDEIRSATRGIADFDRIEG
>Mature_743_residues
PDLLLELFSEEIPARMQAQAADTLRKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLPGRQADSREERKGPRMGAPEGA
IQGFLKSAGLASIDQAKIESDPKKGEFYVAVIERPGKATTEVIAEIVPEIVRTFPWPKSMRWGTGTMRWVRPLHSIVCTF
GTEVEDPEVVRFEVEGIVSGDVTRGHRFLAPEPFAVRRLEDWMAKLEAAKVVADRDRRKAIILNDAKDLALVHGLELVED
EGLLEEVCGLVEWPVTLMGSFEERFLDIPDEVIRATIRANQKCFVLRDPRTGRLANRFILVSNIAATDGGAAIVAGNERV
IRARLSDAKFFWDTDRRTPMADRMSKFDAITFHEKLGSQGARINRIVALAREIAPRVGADVDLTEQAARLAKADLLTEVV
GEFPELQGLMGRYYAQHEKLPPEVAAACEEHYKPQGPSDRVPTAPVSIAVALADKLDTLVGFWAIDEKPTGSKDPYALRR
AALGVIRIILDNNLRLPLRPLLVSASRTILGEEHFERHPHDVFRLETIADKVVSDLLQGLARRLANLPADADVESAERVE
GMTLSGDLADDLLAFFGDRLKVHLRDRGARHDLVDAVFALPGQDDLLLVDRRVAALAAFLETEDGRTLLAGYRRAVNILR
IEEKKDGVAYKGPSEAKLLKEPAEEHLAAAIETVKRQVADAVAREDFAAAMGALAHLRGPVDAFFEGVTVNAPEAALRIN
RLKLLDEIRSATRGIADFDRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=753, Percent_Identity=33.8645418326693, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 82093; Mature: 81962

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELFSEEIPARMQAQAADTLRKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHCCHHHHEEEEECCC
GRQADSREERKGPRMGAPEGAIQGFLKSAGLASIDQAKIESDPKKGEFYVAVIERPGKAT
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHH
TEVIAEIVPEIVRTFPWPKSMRWGTGTMRWVRPLHSIVCTFGTEVEDPEVVRFEVEGIVS
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEE
GDVTRGHRFLAPEPFAVRRLEDWMAKLEAAKVVADRDRRKAIILNDAKDLALVHGLELVE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHC
DEGLLEEVCGLVEWPVTLMGSFEERFLDIPDEVIRATIRANQKCFVLRDPRTGRLANRFI
CCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHCEEE
LVSNIAATDGGAAIVAGNERVIRARLSDAKFFWDTDRRTPMADRMSKFDAITFHEKLGSQ
EEEEEEECCCCEEEEECCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
GARINRIVALAREIAPRVGADVDLTEQAARLAKADLLTEVVGEFPELQGLMGRYYAQHEK
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
LPPEVAAACEEHYKPQGPSDRVPTAPVSIAVALADKLDTLVGFWAIDEKPTGSKDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHH
RAALGVIRIILDNNLRLPLRPLLVSASRTILGEEHFERHPHDVFRLETIADKVVSDLLQG
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LARRLANLPADADVESAERVEGMTLSGDLADDLLAFFGDRLKVHLRDRGARHDLVDAVFA
HHHHHHCCCCCCCCCHHHHHCCCEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
LPGQDDLLLVDRRVAALAAFLETEDGRTLLAGYRRAVNILRIEEKKDGVAYKGPSEAKLL
CCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHH
KEPAEEHLAAAIETVKRQVADAVAREDFAAAMGALAHLRGPVDAFFEGVTVNAPEAALRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHH
NRLKLLDEIRSATRGIADFDRIEG
HHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
PDLLLELFSEEIPARMQAQAADTLRKLVTDALVERGLLYEGAKAFVTPRRLALAVHGLP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHCCHHHHEEEEECCC
GRQADSREERKGPRMGAPEGAIQGFLKSAGLASIDQAKIESDPKKGEFYVAVIERPGKAT
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEECCCCHH
TEVIAEIVPEIVRTFPWPKSMRWGTGTMRWVRPLHSIVCTFGTEVEDPEVVRFEVEGIVS
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEE
GDVTRGHRFLAPEPFAVRRLEDWMAKLEAAKVVADRDRRKAIILNDAKDLALVHGLELVE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHC
DEGLLEEVCGLVEWPVTLMGSFEERFLDIPDEVIRATIRANQKCFVLRDPRTGRLANRFI
CCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHCEEE
LVSNIAATDGGAAIVAGNERVIRARLSDAKFFWDTDRRTPMADRMSKFDAITFHEKLGSQ
EEEEEEECCCCEEEEECCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
GARINRIVALAREIAPRVGADVDLTEQAARLAKADLLTEVVGEFPELQGLMGRYYAQHEK
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
LPPEVAAACEEHYKPQGPSDRVPTAPVSIAVALADKLDTLVGFWAIDEKPTGSKDPYALR
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHH
RAALGVIRIILDNNLRLPLRPLLVSASRTILGEEHFERHPHDVFRLETIADKVVSDLLQG
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LARRLANLPADADVESAERVEGMTLSGDLADDLLAFFGDRLKVHLRDRGARHDLVDAVFA
HHHHHHCCCCCCCCCHHHHHCCCEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
LPGQDDLLLVDRRVAALAAFLETEDGRTLLAGYRRAVNILRIEEKKDGVAYKGPSEAKLL
CCCCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHH
KEPAEEHLAAAIETVKRQVADAVAREDFAAAMGALAHLRGPVDAFFEGVTVNAPEAALRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHH
NRLKLLDEIRSATRGIADFDRIEG
HHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA