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The map label for this gene is proP [H]

Identifier: 158422654

GI number: 158422654

Start: 1156057

End: 1157490

Strand: Direct

Name: proP [H]

Synonym: AZC_1030

Alternate gene names: 158422654

Gene position: 1156057-1157490 (Clockwise)

Preceding gene: 158422653

Following gene: 158422655

Centisome position: 21.53

GC content: 68.27

Gene sequence:

>1434_bases
GTGCAGCGTGCCTTTTGGGGGGCTCGTGATCCGTTCTCCTGCGTTCCGGGGACGGCCGGCGCGGCCGGCCGTGCTGTTCA
AGTGTGGGCCTCGCTCAATGCCGTCCGCAGCGTCCTGCATCGGGGGGCTGTCATGACGAGCCTTGCGGATGCCGGCGCCG
CCGCTGCTCCAGGCCAGGCGGCCCCCGCGCCCCTCTCCCTGCGCCTCGTGCTCGCGGGCAGCATCGGCAACACGCTGGAG
TGGTACGACTTCGGCGCCTATGGCTTTCTCGCGGCCATCTTCGCCCGCAACTTCTTTCCGGCGGATAGCGGCTTTGCCGC
TTTGCTGGCGGCCTATGGCATGATGGCCGCCTCCTTCCTCATGCGCCCGGTGGGCGGCGTCATCTTCGGCCACATCGGGG
ATCGCTACGGGCGCCGCCATGCGCTCTATCTCTCGGCGGCGCTCATGTGCGTCTCCACCGTCGCCATCGGTCTCCTGCCC
ACCTATGCCACCGCCGGCATGGCGGCACCCATCCTCCTTCTGGCTCTGCGTCTGTTACAGGGCGTCTCCATCGGCGGGGA
ATATTCCTCCTCCGTCGTTTTCCTCGCCGAGAGTGCCAAGCCGCGCTGGCGTGGGCTGCTGCTCAGCCTGTGCTCCATGG
GGGCCATGGGCGGCACGATGCTCGGGTCGCTCGTCGGGGCCGCGACCGCCTCCTTCCTGTCGCAGGACGAACTGATCGCC
TGGGGCTGGCGCATGCCCTTCTTCGTCGGCCTGCTGCTGGGCGGCACCACGCTCTGGCTGCGACATGGCCTGCAATCCGC
GCCCGAGCGCTCAACCGAGCAGCCGCGCAGCCCCCTGCTGCAAGCTTTCGCCGACGACTGGCGCGCCATTGTCATCTGCG
CCGTCATCAATCTCGCGGGCGGGGTGAACACCTACACGCTCTTCGTCTATCTCGTGACCTATATGGAGCAGGTGGACGGC
CTCTCCCCCGGCAGTGCGCTGCAGGTCAATACGGCGAGCATGGTCGTCGCCGTCGTGCTCATGCCGATGTTTGCCGCGCT
GTCTGATGTGATCGGCCGCAAGCGTGTGCTCGTGGCGTCGCTGCTCGGCTTCATCCTGTTCGGCTGGCCGCTCTTCCGGC
TGGTGAGCGGAGGCACGGAGGAGGGGGCGATGCTGGGCCAGCTCGGCTTTGCCGTCCTCATCTCGGCCTATACGGGCGTC
TCGCCGGCGGTGCTCGCGGAGCTCTTCAAGCGCGCCACCCGCTGCTCGGCGGCGGCGGTGAGCAACAATGTGAGCCGGGG
CATCGCGGGCGGCACCGCGCCCATGGTGGGCGTCTATCTGGTGAGCGGCCTGCACGACCCCATGGGGCCGGCCCTCTACA
TCATCTGCATCGCGCTGATCTCGCTGATCGCCACGTTGTGCATGACCGACCGGACACGGCTCCCGCTGCCATGA

Upstream 100 bases:

>100_bases
CTGCTGCCTGACATGCCGGCGCGCGCCTCCAGAGCTTTCTCTGGATGGGGTGCGACGAAAGCTTGGCTTTAGGCGGATCC
CGTCGTTACCCTGTTAGGCA

Downstream 100 bases:

>100_bases
GGTGGGACAAGGCTTCCGCCGATTTTGCGGATGATGGATCCGGAGAGGGGGCCGTGTCCGTCGCGATCGTCAGTGAGCGC
CCCTCGGCCCGCCTCGTTGC

Product: proline/betaine transporter 1

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MQRAFWGARDPFSCVPGTAGAAGRAVQVWASLNAVRSVLHRGAVMTSLADAGAAAAPGQAAPAPLSLRLVLAGSIGNTLE
WYDFGAYGFLAAIFARNFFPADSGFAALLAAYGMMAASFLMRPVGGVIFGHIGDRYGRRHALYLSAALMCVSTVAIGLLP
TYATAGMAAPILLLALRLLQGVSIGGEYSSSVVFLAESAKPRWRGLLLSLCSMGAMGGTMLGSLVGAATASFLSQDELIA
WGWRMPFFVGLLLGGTTLWLRHGLQSAPERSTEQPRSPLLQAFADDWRAIVICAVINLAGGVNTYTLFVYLVTYMEQVDG
LSPGSALQVNTASMVVAVVLMPMFAALSDVIGRKRVLVASLLGFILFGWPLFRLVSGGTEEGAMLGQLGFAVLISAYTGV
SPAVLAELFKRATRCSAAAVSNNVSRGIAGGTAPMVGVYLVSGLHDPMGPALYIICIALISLIATLCMTDRTRLPLP

Sequences:

>Translated_477_residues
MQRAFWGARDPFSCVPGTAGAAGRAVQVWASLNAVRSVLHRGAVMTSLADAGAAAAPGQAAPAPLSLRLVLAGSIGNTLE
WYDFGAYGFLAAIFARNFFPADSGFAALLAAYGMMAASFLMRPVGGVIFGHIGDRYGRRHALYLSAALMCVSTVAIGLLP
TYATAGMAAPILLLALRLLQGVSIGGEYSSSVVFLAESAKPRWRGLLLSLCSMGAMGGTMLGSLVGAATASFLSQDELIA
WGWRMPFFVGLLLGGTTLWLRHGLQSAPERSTEQPRSPLLQAFADDWRAIVICAVINLAGGVNTYTLFVYLVTYMEQVDG
LSPGSALQVNTASMVVAVVLMPMFAALSDVIGRKRVLVASLLGFILFGWPLFRLVSGGTEEGAMLGQLGFAVLISAYTGV
SPAVLAELFKRATRCSAAAVSNNVSRGIAGGTAPMVGVYLVSGLHDPMGPALYIICIALISLIATLCMTDRTRLPLP
>Mature_477_residues
MQRAFWGARDPFSCVPGTAGAAGRAVQVWASLNAVRSVLHRGAVMTSLADAGAAAAPGQAAPAPLSLRLVLAGSIGNTLE
WYDFGAYGFLAAIFARNFFPADSGFAALLAAYGMMAASFLMRPVGGVIFGHIGDRYGRRHALYLSAALMCVSTVAIGLLP
TYATAGMAAPILLLALRLLQGVSIGGEYSSSVVFLAESAKPRWRGLLLSLCSMGAMGGTMLGSLVGAATASFLSQDELIA
WGWRMPFFVGLLLGGTTLWLRHGLQSAPERSTEQPRSPLLQAFADDWRAIVICAVINLAGGVNTYTLFVYLVTYMEQVDG
LSPGSALQVNTASMVVAVVLMPMFAALSDVIGRKRVLVASLLGFILFGWPLFRLVSGGTEEGAMLGQLGFAVLISAYTGV
SPAVLAELFKRATRCSAAAVSNNVSRGIAGGTAPMVGVYLVSGLHDPMGPALYIICIALISLIATLCMTDRTRLPLP

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=32.387706855792, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1788942, Length=411, Percent_Identity=32.6034063260341, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=29.7619047619048, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=30.8788598574822, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI87082404, Length=414, Percent_Identity=24.8792270531401, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082231, Length=176, Percent_Identity=32.3863636363636, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49882; Mature: 49882

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRAFWGARDPFSCVPGTAGAAGRAVQVWASLNAVRSVLHRGAVMTSLADAGAAAAPGQA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
APAPLSLRLVLAGSIGNTLEWYDFGAYGFLAAIFARNFFPADSGFAALLAAYGMMAASFL
CCCCEEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
MRPVGGVIFGHIGDRYGRRHALYLSAALMCVSTVAIGLLPTYATAGMAAPILLLALRLLQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVSIGGEYSSSVVFLAESAKPRWRGLLLSLCSMGAMGGTMLGSLVGAATASFLSQDELIA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
WGWRMPFFVGLLLGGTTLWLRHGLQSAPERSTEQPRSPLLQAFADDWRAIVICAVINLAG
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GVNTYTLFVYLVTYMEQVDGLSPGSALQVNTASMVVAVVLMPMFAALSDVIGRKRVLVAS
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFILFGWPLFRLVSGGTEEGAMLGQLGFAVLISAYTGVSPAVLAELFKRATRCSAAAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SNNVSRGIAGGTAPMVGVYLVSGLHDPMGPALYIICIALISLIATLCMTDRTRLPLP
HCCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQRAFWGARDPFSCVPGTAGAAGRAVQVWASLNAVRSVLHRGAVMTSLADAGAAAAPGQA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
APAPLSLRLVLAGSIGNTLEWYDFGAYGFLAAIFARNFFPADSGFAALLAAYGMMAASFL
CCCCEEEEEEEECCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
MRPVGGVIFGHIGDRYGRRHALYLSAALMCVSTVAIGLLPTYATAGMAAPILLLALRLLQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVSIGGEYSSSVVFLAESAKPRWRGLLLSLCSMGAMGGTMLGSLVGAATASFLSQDELIA
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEE
WGWRMPFFVGLLLGGTTLWLRHGLQSAPERSTEQPRSPLLQAFADDWRAIVICAVINLAG
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GVNTYTLFVYLVTYMEQVDGLSPGSALQVNTASMVVAVVLMPMFAALSDVIGRKRVLVAS
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGFILFGWPLFRLVSGGTEEGAMLGQLGFAVLISAYTGVSPAVLAELFKRATRCSAAAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
SNNVSRGIAGGTAPMVGVYLVSGLHDPMGPALYIICIALISLIATLCMTDRTRLPLP
HCCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]