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The map label for this gene is folC [H]

Identifier: 158422649

GI number: 158422649

Start: 1149505

End: 1150812

Strand: Direct

Name: folC [H]

Synonym: AZC_1025

Alternate gene names: 158422649

Gene position: 1149505-1150812 (Clockwise)

Preceding gene: 158422648

Following gene: 158422650

Centisome position: 21.41

GC content: 72.78

Gene sequence:

>1308_bases
GTGAGCCCACTCGGCGCGCTGCTGGAGCGCCTCGCGGCGCTCCACCCCAAGAAGATCGACCTGTCGCTGGAGCGCATGGA
GCGCCTGGCGGCGGCGCTCGGCAATCCCGAGCGCCGGCTGCCGCCCGTCGTCCATGTGGCGGGCACCAACGGCAAGGGCT
CCACCGTCGCCTACCTGCGCGCCGCGCTGGAGGCGGCCGGACGGCGCGTCCACACCTACACCTCGCCACACCTCGTGCGC
TTCAACGAGCGCATCCGCCTCGCCGGTGAACTGGTGGACGATGCGCGGCTGTGCGATGCGCTGGAGCGCGTGGCGGCGGC
CAATGACGGTGGCCCGATCACCTTCTTCGAAGTGACGACGGCGGCCGCCTTCCTCCTGTTCGCGGAGACCCCGGCGGACG
TGCTGCTGCTGGAGGTGGGCCTCGGCGGCCGCCTCGACGCGACTAACATCATCGAGGCGCCGGTCGCGAGCGTCATCACG
CCCATCTCCATCGACCATGTGGAATTCCTCGGCGATACGCTGGAAGCCATTGCCGGTGAGAAGGCGGGCATCATCAAGCC
GCGCGTGCCGGTGATCGTGGGCGAGCAGCCGGACGAGGCGCTGGCGGTGATCGAGCGCACCGCCGCCCGCCGCCGGGCAC
CGCTGTTCGCGCGCGGCCAGCAGTGGACGGTGCAGGAGGAGGCGGGCCGCCTCGTCTATTCCGACGAGGATGGACTGCTG
GACCTGCCGCGCCCGCGTCTCATGGGGCCGCACCAGTTCGACAATGCCGGCCTTGCGGTGGCCACGCTGCGGGCCGTGCC
CGAGTTGCGCGTGCCGCCGCCGGCGCTTGAGGCCGGCATCCGGGGCGCCGACTGGCCCGCCCGTCTCCAGCGCCTGCCCG
TCGGGCCGCTGGTCGCCCGCGCGCCACGCGAGGCGGAGGTCTGGCTCGACGGCGGCCACAATGCCGGCGGCGGTCAGGCG
CTGGCCCATGCGCTGGCGGAACTGGAAGAGCGCGTGCCCCGGCCGCTGGTGCTGGTGGTCGGCATGCTGGCCAACAAGGA
CGTGTCCGGTTTCCTCGGCGCCTTTGCAGGACTCGCCCGCGAGGCCATCTGCGTACCGGTGCCGCCGGAACACAACGGCA
CGGCGCCGGACGACCTCGCACGCATCGCCGCCGGGCTCGGCTTTTCCGCTTCCACGGCGGGCGATGTGGGCGCGGCGCTG
GACGGCCTTGCGCGCTATCCGCTGACGCCGCCACCCCGCGTCCTCGTCTGCGGCTCGCTCTATCTCGCCGGGACGGTGCT
CGCCGCCAACGAGACGCTGCCGAACTGA

Upstream 100 bases:

>100_bases
ACCGCCACCAGATGCGCGAGACGCTGGCCCGCGTCTGTCGTCTCATGATGAAGGCGCCCGGCGTGCCCCCCAAGGGCCGT
CTTCCGGCCCCCGCCGCCGC

Downstream 100 bases:

>100_bases
TTATCTCGACAGGCCAGATGGATAGGGCCGGGTCGTCCGAGCGGCGCCCAACGGCGGTTGCAGATTCTCGCAATGCGCAA
GCCTCGCGCAACCCATGTCT

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSPLGALLERLAALHPKKIDLSLERMERLAAALGNPERRLPPVVHVAGTNGKGSTVAYLRAALEAAGRRVHTYTSPHLVR
FNERIRLAGELVDDARLCDALERVAAANDGGPITFFEVTTAAAFLLFAETPADVLLLEVGLGGRLDATNIIEAPVASVIT
PISIDHVEFLGDTLEAIAGEKAGIIKPRVPVIVGEQPDEALAVIERTAARRRAPLFARGQQWTVQEEAGRLVYSDEDGLL
DLPRPRLMGPHQFDNAGLAVATLRAVPELRVPPPALEAGIRGADWPARLQRLPVGPLVARAPREAEVWLDGGHNAGGGQA
LAHALAELEERVPRPLVLVVGMLANKDVSGFLGAFAGLAREAICVPVPPEHNGTAPDDLARIAAGLGFSASTAGDVGAAL
DGLARYPLTPPPRVLVCGSLYLAGTVLAANETLPN

Sequences:

>Translated_435_residues
MSPLGALLERLAALHPKKIDLSLERMERLAAALGNPERRLPPVVHVAGTNGKGSTVAYLRAALEAAGRRVHTYTSPHLVR
FNERIRLAGELVDDARLCDALERVAAANDGGPITFFEVTTAAAFLLFAETPADVLLLEVGLGGRLDATNIIEAPVASVIT
PISIDHVEFLGDTLEAIAGEKAGIIKPRVPVIVGEQPDEALAVIERTAARRRAPLFARGQQWTVQEEAGRLVYSDEDGLL
DLPRPRLMGPHQFDNAGLAVATLRAVPELRVPPPALEAGIRGADWPARLQRLPVGPLVARAPREAEVWLDGGHNAGGGQA
LAHALAELEERVPRPLVLVVGMLANKDVSGFLGAFAGLAREAICVPVPPEHNGTAPDDLARIAAGLGFSASTAGDVGAAL
DGLARYPLTPPPRVLVCGSLYLAGTVLAANETLPN
>Mature_434_residues
SPLGALLERLAALHPKKIDLSLERMERLAAALGNPERRLPPVVHVAGTNGKGSTVAYLRAALEAAGRRVHTYTSPHLVRF
NERIRLAGELVDDARLCDALERVAAANDGGPITFFEVTTAAAFLLFAETPADVLLLEVGLGGRLDATNIIEAPVASVITP
ISIDHVEFLGDTLEAIAGEKAGIIKPRVPVIVGEQPDEALAVIERTAARRRAPLFARGQQWTVQEEAGRLVYSDEDGLLD
LPRPRLMGPHQFDNAGLAVATLRAVPELRVPPPALEAGIRGADWPARLQRLPVGPLVARAPREAEVWLDGGHNAGGGQAL
AHALAELEERVPRPLVLVVGMLANKDVSGFLGAFAGLAREAICVPVPPEHNGTAPDDLARIAAGLGFSASTAGDVGAALD
GLARYPLTPPPRVLVCGSLYLAGTVLAANETLPN

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=325, Percent_Identity=30.7692307692308, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI66932984, Length=325, Percent_Identity=30.7692307692308, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788654, Length=427, Percent_Identity=35.5971896955504, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71984923, Length=307, Percent_Identity=30.2931596091205, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=307, Percent_Identity=30.2931596091205, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17553150, Length=307, Percent_Identity=30.6188925081433, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=425, Percent_Identity=30.3529411764706, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6324815, Length=337, Percent_Identity=27.299703264095, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24641571, Length=179, Percent_Identity=36.3128491620112, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24581568, Length=205, Percent_Identity=31.219512195122, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45817; Mature: 45686

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLGALLERLAALHPKKIDLSLERMERLAAALGNPERRLPPVVHVAGTNGKGSTVAYLR
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCCCHHHHHH
AALEAAGRRVHTYTSPHLVRFNERIRLAGELVDDARLCDALERVAAANDGGPITFFEVTT
HHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
AAAFLLFAETPADVLLLEVGLGGRLDATNIIEAPVASVITPISIDHVEFLGDTLEAIAGE
HHHHHHEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KAGIIKPRVPVIVGEQPDEALAVIERTAARRRAPLFARGQQWTVQEEAGRLVYSDEDGLL
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEECCCCEEEHHHCCCEEEECCCCCE
DLPRPRLMGPHQFDNAGLAVATLRAVPELRVPPPALEAGIRGADWPARLQRLPVGPLVAR
ECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHC
APREAEVWLDGGHNAGGGQALAHALAELEERVPRPLVLVVGMLANKDVSGFLGAFAGLAR
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EAICVPVPPEHNGTAPDDLARIAAGLGFSASTAGDVGAALDGLARYPLTPPPRVLVCGSL
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCH
YLAGTVLAANETLPN
HHHHEEEECCCCCCC
>Mature Secondary Structure 
SPLGALLERLAALHPKKIDLSLERMERLAAALGNPERRLPPVVHVAGTNGKGSTVAYLR
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCCCHHHHHH
AALEAAGRRVHTYTSPHLVRFNERIRLAGELVDDARLCDALERVAAANDGGPITFFEVTT
HHHHHCCCEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
AAAFLLFAETPADVLLLEVGLGGRLDATNIIEAPVASVITPISIDHVEFLGDTLEAIAGE
HHHHHHEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
KAGIIKPRVPVIVGEQPDEALAVIERTAARRRAPLFARGQQWTVQEEAGRLVYSDEDGLL
CCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEECCCCEEEHHHCCCEEEECCCCCE
DLPRPRLMGPHQFDNAGLAVATLRAVPELRVPPPALEAGIRGADWPARLQRLPVGPLVAR
ECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHCCCCHHHHC
APREAEVWLDGGHNAGGGQALAHALAELEERVPRPLVLVVGMLANKDVSGFLGAFAGLAR
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EAICVPVPPEHNGTAPDDLARIAAGLGFSASTAGDVGAALDGLARYPLTPPPRVLVCGSL
CCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCH
YLAGTVLAANETLPN
HHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]