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The map label for this gene is gyrB [H]

Identifier: 158422633

GI number: 158422633

Start: 1131714

End: 1134140

Strand: Reverse

Name: gyrB [H]

Synonym: AZC_1009

Alternate gene names: 158422633

Gene position: 1134140-1131714 (Counterclockwise)

Preceding gene: 158422634

Following gene: 158422627

Centisome position: 21.12

GC content: 67.2

Gene sequence:

>2427_bases
ATGGCCGAACCCATGCCCGCGGCGTCCCCCGAGGAATATGGCGCCCAGTCCATTCAGGTCCTGAAGGGTCTGGACGCCGT
GCGCAAGCGCCCCGGCATGTACATCGGCGACACCGACGACGGCTCCGGTCTCCACCACATGGTCTATGAGGTGGTGGACA
ATGCCATCGACGAGGCGCTGGCCGGCCACGCCAAGGAAGTCACCGTCACGCTGAATGCCGACGGCTCCGTGACCGTGACC
GACGACGGGCGCGGCATTCCTACCGGCATCCACGAGGAAGAGGGCGTCTCGGCGGCCGAGGTCATCATGACCCAGCTCCA
CGCCGGCGGTAAGTTCAACCAGAATTCCTACAAGGTTTCCGGCGGCCTGCACGGCGTCGGCGTCTCGGTGGTGAACGCGC
TGTCCACCTATCTCGACCTGACCATCTGGCGGGACGGGAAGGAGCATTACATGCGCTTCCGCCACGGCGACGCGGAAGCG
CCGCTGAAGGTGGTGGGCGATGCCCCGGCGGGCAAGCGCGGCACGAAGGTGACCTTCCTGCCCTCGCCGGAGACTTTCAC
CAAGATCGAGTTCGATTACGCCACACTGGAGCACCGGCTGCGCGAACTCGCCTTCCTGAATTCCGGCGTGCTGATTACCC
TCACCGACGCGCGCGGCCCCGAGCCGAAGCGCGAGGAATTGCACTATGAGGGCGGCGTCGAGGCCTTCGTGCGCTATCTC
GACCGCTCCAAGCAGGCGCTGCTGCAGAAGCCGGTGGTGATCCGCGCCGAGCGCGACGGCATCACCGTGGAAGCCGCCTT
GTCGTGGAACGACGGGTATCACGAGAACATCCTCTGCTTCACCAACAACATCCCGCAGCGCGATCGCGGCACCCACTTCA
CAGGCTTTGCCGCCGCCCTCACCCGGCAGGTGATCGGCTATGCGGACCGTTCCGGCATCGCCAAGAAGGAGAAGGTGGAC
CCCACCGGCGAGGACTGCCGCGAGGGCCTGACCGCCGTTCTCTCCGTGAAGGTGCCGGACCCGAAATTCTCGTCCCAGAC
CAAGGACAAGCTCGTCTCCTCCGAGGTGCGCCCGGTGGTGGAAAGCCTGGTGGGTGACGCGCTCTCCACCTGGTTCGAGG
AGAACCCGAACGAAGGCAAGTCGGTGGTGGGCAAGGTGGTGGAAGCCGCCGCCGCCCGCGAAGCCGCCCGCCGCGCCCGC
GACCTCACCCGCCGCAAGAACCCGCTGGACGTGGCTTCCCTGCCCGGCAAGCTCGCCGACTGCCAGGAGCGCGACCCGGC
CAAGTCCGAAATCTTCATCGTCGAGGGTGACTCGGCCGGCGGCTCCGCCAAGCAGGGCCGCGACCGCGCCTTCCAGGCGG
TGCTGCCGCTCCGCGGCAAGATCCTCAACGTGGAGCGCGCGCGCTTCGACAAGATGCTGTCGTCGGACCAGATCGGCACG
CTCATCACCGCGCTCGGCACCGGCATCGGCCGCGATGACTTCGACATCGCCAAGCTGCGCTACCACAAGATCATCATCAT
GACCGACGCGGACGTGGACGGCTCCCACATCCGCACCCTGCTGCTCACCTTCTTCTTCCGGCAGATGCCGGAGGTGCTTG
AGCGCGGGCACATCTACATCGCCCAGCCGCCGCTGTTCAAAGTCGCGCGCGGCAAGTCCGAGACCTACATCAAGGACGAG
AAGGCGCTGGAAGACTATCTCATCACCGCCGGCCTGGAGGACGCCGCCCTCCATGCCGCTTCCGGCGAGGTCCGCACGGG
CGCGGATCTTGCGGCCGTGGTGGATGTCACCCGCCACATGCGCGGCACGCTGCTGAACCTCCATCCGCGCTATAACCGCT
CGGTGGCCGAGCAGGCCGCCTTCGCCGGAGCGCTGGTGCCCGGCATTCTCGATGACGACGCGACGGCCACCGCCGCCGCC
CAGCGGATTGCCGCCCGGCTCAACATCCTCGCGGAAGAGACAGAGCGCACCTGGGAAGGCGCGGCGGACGACACGGGCAT
CCGCCTCACCCGCAGCGTGCGCGGCGTGCTGGAAACGAGCGTGCTCGACAACGGCTTCATAGCCTCGCCCGAGGCCCGCC
GGCTGGACTCGCTGGCCAGGCAGCTCGACGGCATCTTCACCGGCCCCTCGCTGTTGCGCCGCAAGAGCGAGGAGCATTCG
GTCTTCGGCGCCGTGGACCTGTTCGACGCCATCACCGATGCCGGCCGCAAGGGCCTCTCGCTCCAGCGCTACAAAGGCCT
GGGCGAGATGAACCCGGAGCAGTTGTGGGAGACCACGCTCGACATCAACGCCCGCTCGCTGCTGCAGGTGAAGGTGAAGG
AAGTGGACGCCGCCGACGACCTCTTCAACCGCCTGATGGGCGACGTGGTGGAGCCGCGCCGCGACTTCATCCAGGAAAAT
GCCCTCAAGGCCAGCGTCGACGTCTGA

Upstream 100 bases:

>100_bases
GAACCGTTTTGGCGGGCACCGCGGTGGCGGTTGCCACCAAATAACCGCGGCTTTGCGGTACAAGACTCCTCAAGCGTTTC
AACGATTCGAGAAATCCAAC

Downstream 100 bases:

>100_bases
CGGCGGCGGGATTTCGCGTTAAGGGCGCGGCGACCTTGCCGCCGCGCCCCCTCTGCCGCCTCACCATTCCAGTTGTGCCG
GGGCCGTGGAGGTGGGGCCA

Product: DNA gyrase B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MAEPMPAASPEEYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHAKEVTVTLNADGSVTVT
DDGRGIPTGIHEEEGVSAAEVIMTQLHAGGKFNQNSYKVSGGLHGVGVSVVNALSTYLDLTIWRDGKEHYMRFRHGDAEA
PLKVVGDAPAGKRGTKVTFLPSPETFTKIEFDYATLEHRLRELAFLNSGVLITLTDARGPEPKREELHYEGGVEAFVRYL
DRSKQALLQKPVVIRAERDGITVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAALTRQVIGYADRSGIAKKEKVD
PTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLVGDALSTWFEENPNEGKSVVGKVVEAAAAREAARRAR
DLTRRKNPLDVASLPGKLADCQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSDQIGT
LITALGTGIGRDDFDIAKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVLERGHIYIAQPPLFKVARGKSETYIKDE
KALEDYLITAGLEDAALHAASGEVRTGADLAAVVDVTRHMRGTLLNLHPRYNRSVAEQAAFAGALVPGILDDDATATAAA
QRIAARLNILAEETERTWEGAADDTGIRLTRSVRGVLETSVLDNGFIASPEARRLDSLARQLDGIFTGPSLLRRKSEEHS
VFGAVDLFDAITDAGRKGLSLQRYKGLGEMNPEQLWETTLDINARSLLQVKVKEVDAADDLFNRLMGDVVEPRRDFIQEN
ALKASVDV

Sequences:

>Translated_808_residues
MAEPMPAASPEEYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHAKEVTVTLNADGSVTVT
DDGRGIPTGIHEEEGVSAAEVIMTQLHAGGKFNQNSYKVSGGLHGVGVSVVNALSTYLDLTIWRDGKEHYMRFRHGDAEA
PLKVVGDAPAGKRGTKVTFLPSPETFTKIEFDYATLEHRLRELAFLNSGVLITLTDARGPEPKREELHYEGGVEAFVRYL
DRSKQALLQKPVVIRAERDGITVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAALTRQVIGYADRSGIAKKEKVD
PTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLVGDALSTWFEENPNEGKSVVGKVVEAAAAREAARRAR
DLTRRKNPLDVASLPGKLADCQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSDQIGT
LITALGTGIGRDDFDIAKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVLERGHIYIAQPPLFKVARGKSETYIKDE
KALEDYLITAGLEDAALHAASGEVRTGADLAAVVDVTRHMRGTLLNLHPRYNRSVAEQAAFAGALVPGILDDDATATAAA
QRIAARLNILAEETERTWEGAADDTGIRLTRSVRGVLETSVLDNGFIASPEARRLDSLARQLDGIFTGPSLLRRKSEEHS
VFGAVDLFDAITDAGRKGLSLQRYKGLGEMNPEQLWETTLDINARSLLQVKVKEVDAADDLFNRLMGDVVEPRRDFIQEN
ALKASVDV
>Mature_807_residues
AEPMPAASPEEYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHAKEVTVTLNADGSVTVTD
DGRGIPTGIHEEEGVSAAEVIMTQLHAGGKFNQNSYKVSGGLHGVGVSVVNALSTYLDLTIWRDGKEHYMRFRHGDAEAP
LKVVGDAPAGKRGTKVTFLPSPETFTKIEFDYATLEHRLRELAFLNSGVLITLTDARGPEPKREELHYEGGVEAFVRYLD
RSKQALLQKPVVIRAERDGITVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAALTRQVIGYADRSGIAKKEKVDP
TGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVESLVGDALSTWFEENPNEGKSVVGKVVEAAAAREAARRARD
LTRRKNPLDVASLPGKLADCQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSDQIGTL
ITALGTGIGRDDFDIAKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVLERGHIYIAQPPLFKVARGKSETYIKDEK
ALEDYLITAGLEDAALHAASGEVRTGADLAAVVDVTRHMRGTLLNLHPRYNRSVAEQAAFAGALVPGILDDDATATAAAQ
RIAARLNILAEETERTWEGAADDTGIRLTRSVRGVLETSVLDNGFIASPEARRLDSLARQLDGIFTGPSLLRRKSEEHSV
FGAVDLFDAITDAGRKGLSLQRYKGLGEMNPEQLWETTLDINARSLLQVKVKEVDAADDLFNRLMGDVVEPRRDFIQENA
LKASVDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=566, Percent_Identity=24.5583038869258, Blast_Score=122, Evalue=2e-27,
Organism=Homo sapiens, GI19913406, Length=564, Percent_Identity=24.113475177305, Blast_Score=116, Evalue=7e-26,
Organism=Escherichia coli, GI48994957, Length=801, Percent_Identity=58.5518102372035, Blast_Score=887, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=558, Percent_Identity=39.9641577060932, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=536, Percent_Identity=24.6268656716418, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=512, Percent_Identity=24.21875, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI212645657, Length=174, Percent_Identity=30.4597701149425, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=600, Percent_Identity=25.5, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI17136538, Length=535, Percent_Identity=25.607476635514, Blast_Score=127, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 88237; Mature: 88105

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPMPAASPEEYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEAL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
AGHAKEVTVTLNADGSVTVTDDGRGIPTGIHEEEGVSAAEVIMTQLHAGGKFNQNSYKVS
CCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGVSVVNALSTYLDLTIWRDGKEHYMRFRHGDAEAPLKVVGDAPAGKRGTKVTFL
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEC
PSPETFTKIEFDYATLEHRLRELAFLNSGVLITLTDARGPEPKREELHYEGGVEAFVRYL
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCCCHHHHHHHH
DRSKQALLQKPVVIRAERDGITVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAAL
HHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
TRQVIGYADRSGIAKKEKVDPTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVV
HHHHHHHHCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ESLVGDALSTWFEENPNEGKSVVGKVVEAAAAREAARRARDLTRRKNPLDVASLPGKLAD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHC
CQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSDQIGT
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHCCCHHHH
LITALGTGIGRDDFDIAKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVLERGHIYI
HHHHHHCCCCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLFKVARGKSETYIKDEKALEDYLITAGLEDAALHAASGEVRTGADLAAVVDVTRHM
ECCCHHHHHCCCCCCEECHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHH
RGTLLNLHPRYNRSVAEQAAFAGALVPGILDDDATATAAAQRIAARLNILAEETERTWEG
HCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
AADDTGIRLTRSVRGVLETSVLDNGFIASPEARRLDSLARQLDGIFTGPSLLRRKSEEHS
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHH
VFGAVDLFDAITDAGRKGLSLQRYKGLGEMNPEQLWETTLDINARSLLQVKVKEVDAADD
HHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHEEHHHHCCCHHH
LFNRLMGDVVEPRRDFIQENALKASVDV
HHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEPMPAASPEEYGAQSIQVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
AGHAKEVTVTLNADGSVTVTDDGRGIPTGIHEEEGVSAAEVIMTQLHAGGKFNQNSYKVS
CCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGVSVVNALSTYLDLTIWRDGKEHYMRFRHGDAEAPLKVVGDAPAGKRGTKVTFL
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEC
PSPETFTKIEFDYATLEHRLRELAFLNSGVLITLTDARGPEPKREELHYEGGVEAFVRYL
CCCCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHCCCCCCHHHHHHHH
DRSKQALLQKPVVIRAERDGITVEAALSWNDGYHENILCFTNNIPQRDRGTHFTGFAAAL
HHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHHHHH
TRQVIGYADRSGIAKKEKVDPTGEDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVV
HHHHHHHHCCCCCCCCCCCCCCCHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
ESLVGDALSTWFEENPNEGKSVVGKVVEAAAAREAARRARDLTRRKNPLDVASLPGKLAD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHC
CQERDPAKSEIFIVEGDSAGGSAKQGRDRAFQAVLPLRGKILNVERARFDKMLSSDQIGT
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHCCCHHHH
LITALGTGIGRDDFDIAKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVLERGHIYI
HHHHHHCCCCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLFKVARGKSETYIKDEKALEDYLITAGLEDAALHAASGEVRTGADLAAVVDVTRHM
ECCCHHHHHCCCCCCEECHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHH
RGTLLNLHPRYNRSVAEQAAFAGALVPGILDDDATATAAAQRIAARLNILAEETERTWEG
HCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
AADDTGIRLTRSVRGVLETSVLDNGFIASPEARRLDSLARQLDGIFTGPSLLRRKSEEHS
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCHHHH
VFGAVDLFDAITDAGRKGLSLQRYKGLGEMNPEQLWETTLDINARSLLQVKVKEVDAADD
HHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHEEHHHHCCCHHH
LFNRLMGDVVEPRRDFIQENALKASVDV
HHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]