Click here to switch to the map view.

The map label for this gene is lysE [C]

Identifier: 158422634

GI number: 158422634

Start: 1134197

End: 1134859

Strand: Reverse

Name: lysE [C]

Synonym: AZC_1010

Alternate gene names: 158422634

Gene position: 1134859-1134197 (Counterclockwise)

Preceding gene: 158422635

Following gene: 158422633

Centisome position: 21.13

GC content: 68.93

Gene sequence:

>663_bases
TTGCCGGGCGGGGTTGACCTCGTGCGCGCGCGGGCGCACACACGGGGCATGGATTTTGCCACCCTCTCGCTCTTTGCGGT
TGCCCTTGCGGTCGCGGCCGTGATCCCCGGCCCCGCCGTGGTGGCACTCGTGACGCGGACGCTGGCGCGGGGCACGCGCG
GCGCACTCACCTTCTCCGCCGCCCTCATCCTCGGGGACGTGCTGTGGCTCGCCGCCGCCGTGCTCGGCCTCTCCGCGCTC
GCTGCAACGCTGGGCAGCTTCTTCTTCCTCGTGCGACTGGCGGGCGCCGCCTATCTGCTGCTGCTCGCCTGGCGGCTGTG
GCGCGATCCCGGCCATCCGGTCGATCCCACGCAGGTGCCGCAGACGGAGACGCGTGGCAGCCTGTTCGGCGCAGGGCTCG
CGCTCTCCATGGGCAATCCCAAGACCATGGCCTTCTACCTCGCCATGCTGCCGGCAATGCTGGATCTGGACCATCTGACC
GCGGTCGGCTTTGCCGAGATGGCGGTCGCCATCGTGCTGGTCCTGACGGGCGTTTTCGCCTTTTACGTGCACATGGCCAA
CCGTGCGCGCCACCTCATGACGCGCGCCTCGGCCATGAAGCTCCTAAATCGTGTGTGCGGAACCGTTTTGGCGGGCACCG
CGGTGGCGGTTGCCACCAAATAA

Upstream 100 bases:

>100_bases
ACCTTTACGAGCGCGTGCCGCTGGGCACCCGCGTGATCGTCATCAACAACTGATCCTGACGGCGCCGGCTTCGGCGCCGT
TTTTCTTTGCCTTCAAGGCT

Downstream 100 bases:

>100_bases
CCGCGGCTTTGCGGTACAAGACTCCTCAAGCGTTTCAACGATTCGAGAAATCCAACATGGCCGAACCCATGCCCGCGGCG
TCCCCCGAGGAATATGGCGC

Product: LysE family protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MPGGVDLVRARAHTRGMDFATLSLFAVALAVAAVIPGPAVVALVTRTLARGTRGALTFSAALILGDVLWLAAAVLGLSAL
AATLGSFFFLVRLAGAAYLLLLAWRLWRDPGHPVDPTQVPQTETRGSLFGAGLALSMGNPKTMAFYLAMLPAMLDLDHLT
AVGFAEMAVAIVLVLTGVFAFYVHMANRARHLMTRASAMKLLNRVCGTVLAGTAVAVATK

Sequences:

>Translated_220_residues
MPGGVDLVRARAHTRGMDFATLSLFAVALAVAAVIPGPAVVALVTRTLARGTRGALTFSAALILGDVLWLAAAVLGLSAL
AATLGSFFFLVRLAGAAYLLLLAWRLWRDPGHPVDPTQVPQTETRGSLFGAGLALSMGNPKTMAFYLAMLPAMLDLDHLT
AVGFAEMAVAIVLVLTGVFAFYVHMANRARHLMTRASAMKLLNRVCGTVLAGTAVAVATK
>Mature_219_residues
PGGVDLVRARAHTRGMDFATLSLFAVALAVAAVIPGPAVVALVTRTLARGTRGALTFSAALILGDVLWLAAAVLGLSALA
ATLGSFFFLVRLAGAAYLLLLAWRLWRDPGHPVDPTQVPQTETRGSLFGAGLALSMGNPKTMAFYLAMLPAMLDLDHLTA
VGFAEMAVAIVLVLTGVFAFYVHMANRARHLMTRASAMKLLNRVCGTVLAGTAVAVATK

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=175, Percent_Identity=26.8571428571429, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22985; Mature: 22854

Theoretical pI: Translated: 11.32; Mature: 11.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGGVDLVRARAHTRGMDFATLSLFAVALAVAAVIPGPAVVALVTRTLARGTRGALTFSA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHH
ALILGDVLWLAAAVLGLSALAATLGSFFFLVRLAGAAYLLLLAWRLWRDPGHPVDPTQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QTETRGSLFGAGLALSMGNPKTMAFYLAMLPAMLDLDHLTAVGFAEMAVAIVLVLTGVFA
CCHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYVHMANRARHLMTRASAMKLLNRVCGTVLAGTAVAVATK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
PGGVDLVRARAHTRGMDFATLSLFAVALAVAAVIPGPAVVALVTRTLARGTRGALTFSA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHH
ALILGDVLWLAAAVLGLSALAATLGSFFFLVRLAGAAYLLLLAWRLWRDPGHPVDPTQVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
QTETRGSLFGAGLALSMGNPKTMAFYLAMLPAMLDLDHLTAVGFAEMAVAIVLVLTGVFA
CCHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYVHMANRARHLMTRASAMKLLNRVCGTVLAGTAVAVATK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]