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The map label for this gene is pcaF [H]

Identifier: 158422620

GI number: 158422620

Start: 1112918

End: 1114123

Strand: Direct

Name: pcaF [H]

Synonym: AZC_0996

Alternate gene names: 158422620

Gene position: 1112918-1114123 (Clockwise)

Preceding gene: 158422619

Following gene: 158422621

Centisome position: 20.73

GC content: 69.98

Gene sequence:

>1206_bases
ATGACCGAAGCTTATATCTGCGACTATATCCGCACCCCCATCGGCCGCTTCGGCGGCGCGCTGTCCGCCGTGCGGGCCGA
CGATCTCGGGGCCATTCCGCTGAAGGCGCTCGTCGCCCGCAATGCCGGTCTCGACTGGGAGAAGGTGGACGACGTGATCT
TCGGCTGCGCCAACCAGGCTGGCGAGGACAATCGCAACGTCGCCCGCATGTCGCTGCTGCTGGCGGGCCTCCCGCTCTCC
ATCGGCGGCACCACCATGAACCGCCTGTGCGGCTCGGGCATGGATGCGGTGGCGACCGCCGCCCGCGCCATCAAGGCGGG
CGAGGCGGACCTCATCATCGCCGGCGGCGTGGAGAGCATGAGCCGCGCGCCCCTCGTCATGCCGAAGGCGGACGCAGCCT
TCTCCCGCCGCGCGGAAATCTACGACACCACCATCGGCTGGCGCTTCGTCAATCCGCTGATGAAGGAGATGTATGGCGTC
GATTCCATGCCGGAGACCGGCGACAATGTCGCCATCGACTACAAGGTCTCCCGCGAGGATCAGGACGCCTTCGCCGTCCG
CAGCCAGGAGAAGGCCTCGGCCGCTCAGGCCAATGGCCGGCTGGCGCGCGAAATCACGCCCGTGACCCTGCCGCAGAAGA
AGGGTGATCCGGTCGTGGTGGAGCGCGACGAGCATCCCCGCGCCACGAGCCTTGAGGCGCTCGCCAAGCTCAAGCCCGTG
AACCGCATGGCGGGAGCGACCGTCACCGCGGGCAATGCTTCGGGCGTGAACGACGGCGCCGCGGCGCTCATCATCGCCTC
GGAAGCCGCCGCGAAGGCCCATGGCCTGACGCCCATCGCCCGCGTGCTCGGCGGCGCGGTGGCCGGCGTTCCGCCCCGCG
TCATGGGCATCGGCCCGGCGCCGGCCAGCCAGAAGCTCATGGCCCGCCTCGGTCTCACGCAGGACCAGTTCGACGTGATC
GAACTGAACGAGGCCTTCGCCTCTCAGGGCCTCGCCACGCTGCGCCTGCTGGGCATTGCGGATGACGATGCGCGCGTGAA
CTGCAATGGCGGCGCCATCGCTCTCGGCCATCCGCTCGGCATGTCCGGCGCCCGCATCACCGGCACCGCGGCACTGGAAC
TGACCGTGGGCGGCGGCCGCCGGGCGCTGGCGACCATGTGCATCGGCGTCGGCCAGGGCATCGCGGTGGCGCTGGAGCGC
GTCTGA

Upstream 100 bases:

>100_bases
CGCTCTCCCTTGAAGAGCTTCAGGCGATGACCGGCGCGCGCCTCCACATCGACGGCCCGGTGGCCGATCTCGTCGCGCCC
GAGCTGTGAGCGGGGATGCC

Downstream 100 bases:

>100_bases
CCCCTCTCGAAAAGGGTGTTCGGGGCGTCGGTGGGCTCGGGCCTGCCGGCGCCCCTTTTCTTTGGTGCGCCCCTCCGCAT
GACTGCGGCCAAACGCCCGA

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTEAYICDYIRTPIGRFGGALSAVRADDLGAIPLKALVARNAGLDWEKVDDVIFGCANQAGEDNRNVARMSLLLAGLPLS
IGGTTMNRLCGSGMDAVATAARAIKAGEADLIIAGGVESMSRAPLVMPKADAAFSRRAEIYDTTIGWRFVNPLMKEMYGV
DSMPETGDNVAIDYKVSREDQDAFAVRSQEKASAAQANGRLAREITPVTLPQKKGDPVVVERDEHPRATSLEALAKLKPV
NRMAGATVTAGNASGVNDGAAALIIASEAAAKAHGLTPIARVLGGAVAGVPPRVMGIGPAPASQKLMARLGLTQDQFDVI
ELNEAFASQGLATLRLLGIADDDARVNCNGGAIALGHPLGMSGARITGTAALELTVGGGRRALATMCIGVGQGIAVALER
V

Sequences:

>Translated_401_residues
MTEAYICDYIRTPIGRFGGALSAVRADDLGAIPLKALVARNAGLDWEKVDDVIFGCANQAGEDNRNVARMSLLLAGLPLS
IGGTTMNRLCGSGMDAVATAARAIKAGEADLIIAGGVESMSRAPLVMPKADAAFSRRAEIYDTTIGWRFVNPLMKEMYGV
DSMPETGDNVAIDYKVSREDQDAFAVRSQEKASAAQANGRLAREITPVTLPQKKGDPVVVERDEHPRATSLEALAKLKPV
NRMAGATVTAGNASGVNDGAAALIIASEAAAKAHGLTPIARVLGGAVAGVPPRVMGIGPAPASQKLMARLGLTQDQFDVI
ELNEAFASQGLATLRLLGIADDDARVNCNGGAIALGHPLGMSGARITGTAALELTVGGGRRALATMCIGVGQGIAVALER
V
>Mature_400_residues
TEAYICDYIRTPIGRFGGALSAVRADDLGAIPLKALVARNAGLDWEKVDDVIFGCANQAGEDNRNVARMSLLLAGLPLSI
GGTTMNRLCGSGMDAVATAARAIKAGEADLIIAGGVESMSRAPLVMPKADAAFSRRAEIYDTTIGWRFVNPLMKEMYGVD
SMPETGDNVAIDYKVSREDQDAFAVRSQEKASAAQANGRLAREITPVTLPQKKGDPVVVERDEHPRATSLEALAKLKPVN
RMAGATVTAGNASGVNDGAAALIIASEAAAKAHGLTPIARVLGGAVAGVPPRVMGIGPAPASQKLMARLGLTQDQFDVIE
LNEAFASQGLATLRLLGIADDDARVNCNGGAIALGHPLGMSGARITGTAALELTVGGGRRALATMCIGVGQGIAVALERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=391, Percent_Identity=42.4552429667519, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=40.0510204081633, Blast_Score=252, Evalue=5e-67,
Organism=Homo sapiens, GI4557237, Length=405, Percent_Identity=36.0493827160494, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=38.5964912280702, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.8501170960187, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI194353979, Length=287, Percent_Identity=31.0104529616725, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=66.4179104477612, Blast_Score=516, Evalue=1e-148,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=47.029702970297, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=43.3915211970075, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI48994986, Length=411, Percent_Identity=41.6058394160584, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=31.7073170731707, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=42.2885572139304, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI25147385, Length=406, Percent_Identity=34.4827586206897, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=34.7290640394089, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=32.7906976744186, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=406, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=37.468982630273, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322031, Length=395, Percent_Identity=35.6962025316456, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=41.2935323383085, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI17648125, Length=404, Percent_Identity=40.0990099009901, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24640423, Length=401, Percent_Identity=34.4139650872818, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.1320754716981, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24585051, Length=398, Percent_Identity=23.1155778894472, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921506, Length=354, Percent_Identity=23.1638418079096, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41436; Mature: 41305

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAYICDYIRTPIGRFGGALSAVRADDLGAIPLKALVARNAGLDWEKVDDVIFGCANQA
CCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCC
GEDNRNVARMSLLLAGLPLSIGGTTMNRLCGSGMDAVATAARAIKAGEADLIIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPLVMPKADAAFSRRAEIYDTTIGWRFVNPLMKEMYGVDSMPETGDNVAIDYKVSRED
CCCCEEECCCCHHHHCCCHHEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCC
QDAFAVRSQEKASAAQANGRLAREITPVTLPQKKGDPVVVERDEHPRATSLEALAKLKPV
CHHHHHHCHHHHHHHHHCCCEEHEECCEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCH
NRMAGATVTAGNASGVNDGAAALIIASEAAAKAHGLTPIARVLGGAVAGVPPRVMGIGPA
HHHCCCEEECCCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHCCHHCCCCCCEEECCCC
PASQKLMARLGLTQDQFDVIELNEAFASQGLATLRLLGIADDDARVNCNGGAIALGHPLG
CHHHHHHHHHCCCCCCCCEEEHHHHHHHCCCEEEEEEEECCCCCEEECCCCEEEECCCCC
MSGARITGTAALELTVGGGRRALATMCIGVGQGIAVALERV
CCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
TEAYICDYIRTPIGRFGGALSAVRADDLGAIPLKALVARNAGLDWEKVDDVIFGCANQA
CCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCC
GEDNRNVARMSLLLAGLPLSIGGTTMNRLCGSGMDAVATAARAIKAGEADLIIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SRAPLVMPKADAAFSRRAEIYDTTIGWRFVNPLMKEMYGVDSMPETGDNVAIDYKVSRED
CCCCEEECCCCHHHHCCCHHEECCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCC
QDAFAVRSQEKASAAQANGRLAREITPVTLPQKKGDPVVVERDEHPRATSLEALAKLKPV
CHHHHHHCHHHHHHHHHCCCEEHEECCEECCCCCCCCEEEECCCCCCHHHHHHHHHHCCH
NRMAGATVTAGNASGVNDGAAALIIASEAAAKAHGLTPIARVLGGAVAGVPPRVMGIGPA
HHHCCCEEECCCCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHCCHHCCCCCCEEECCCC
PASQKLMARLGLTQDQFDVIELNEAFASQGLATLRLLGIADDDARVNCNGGAIALGHPLG
CHHHHHHHHHCCCCCCCCEEEHHHHHHHCCCEEEEEEEECCCCCEEECCCCEEEECCCCC
MSGARITGTAALELTVGGGRRALATMCIGVGQGIAVALERV
CCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]