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The map label for this gene is pcaJ [H]

Identifier: 158422619

GI number: 158422619

Start: 1112217

End: 1112906

Strand: Direct

Name: pcaJ [H]

Synonym: AZC_0995

Alternate gene names: 158422619

Gene position: 1112217-1112906 (Clockwise)

Preceding gene: 158422618

Following gene: 158422620

Centisome position: 20.71

GC content: 66.09

Gene sequence:

>690_bases
ATGACTGAGACCCTTCAGAACGACGGCAAGCTGTCGAACGCCCAGATTGCCTGGCGCGCGGCGCAGGACATTGCCGATGG
CGCCTATGTGAACCTCGGCATCGGCTTCCCCGAGATGGTGGCCAATTATCCGGTCGAGGGCCGCGAGGTGATCTTCCACA
CCGAGAACGGCATCCTCAATTTCGGCGCGCCGCCGCCGGCCGGTCAGGAAGACTGGGACCTTATCAATGCCGGCAAGAAG
GCGGTGACGCTGCGGCCGGGTGCGGCCTTCTTCCACCATGCGGACAGCTTCGCCATGGTGCGCGGCGGGCATCTGGATGT
GGCCATCCTCGGCGCCTATCAGGTGTCGCAGAATGGCGATCTCGCCAACTGGCGCGTCGGCAGCAAGGGCGTGCCGGCGG
TGGGCGGCGCCATGGATCTGGTGCATGGGGCGAAGCAGGTCTTCGTCATCACCGAGCACCAGACCAAGTCCGGCGAGCCC
AAGCTCGTGGAAAGCTGCGCCTTTCCGCTGACCGGCGTCGCCTGCGTCACCCGCGTCTATACGAGCCACGCGGTGGTGGA
TATTCGCGGCGGCCGCTTCATCCTGCGCGAGAAGATCCCGACGCTCTCCCTTGAAGAGCTTCAGGCGATGACCGGCGCGC
GCCTCCACATCGACGGCCCGGTGGCCGATCTCGTCGCGCCCGAGCTGTGA

Upstream 100 bases:

>100_bases
CCCGAGCACGTCATCACACCCGGCATCTTCGTGGACCGGGTGGTCGAGGTCGCCGATGCGCGACAGGAAGAGGACCTCAT
CCGGGCGGGAGCGGCCTACA

Downstream 100 bases:

>100_bases
GCGGGGATGCCATGACCGAAGCTTATATCTGCGACTATATCCGCACCCCCATCGGCCGCTTCGGCGGCGCGCTGTCCGCC
GTGCGGGCCGACGATCTCGG

Product: 3-oxoadipate CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTETLQNDGKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVANYPVEGREVIFHTENGILNFGAPPPAGQEDWDLINAGKK
AVTLRPGAAFFHHADSFAMVRGGHLDVAILGAYQVSQNGDLANWRVGSKGVPAVGGAMDLVHGAKQVFVITEHQTKSGEP
KLVESCAFPLTGVACVTRVYTSHAVVDIRGGRFILREKIPTLSLEELQAMTGARLHIDGPVADLVAPEL

Sequences:

>Translated_229_residues
MTETLQNDGKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVANYPVEGREVIFHTENGILNFGAPPPAGQEDWDLINAGKK
AVTLRPGAAFFHHADSFAMVRGGHLDVAILGAYQVSQNGDLANWRVGSKGVPAVGGAMDLVHGAKQVFVITEHQTKSGEP
KLVESCAFPLTGVACVTRVYTSHAVVDIRGGRFILREKIPTLSLEELQAMTGARLHIDGPVADLVAPEL
>Mature_228_residues
TETLQNDGKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVANYPVEGREVIFHTENGILNFGAPPPAGQEDWDLINAGKKA
VTLRPGAAFFHHADSFAMVRGGHLDVAILGAYQVSQNGDLANWRVGSKGVPAVGGAMDLVHGAKQVFVITEHQTKSGEPK
LVESCAFPLTGVACVTRVYTSHAVVDIRGGRFILREKIPTLSLEELQAMTGARLHIDGPVADLVAPEL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=204, Percent_Identity=46.5686274509804, Blast_Score=184, Evalue=8e-47,
Organism=Homo sapiens, GI4557817, Length=205, Percent_Identity=45.3658536585366, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=42.7184466019417, Blast_Score=161, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17531517, Length=210, Percent_Identity=45.2380952380952, Blast_Score=176, Evalue=7e-45,
Organism=Drosophila melanogaster, GI21358325, Length=205, Percent_Identity=43.4146341463415, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24655794, Length=205, Percent_Identity=43.4146341463415, Blast_Score=175, Evalue=3e-44,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 24420; Mature: 24289

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLQNDGKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVANYPVEGREVIFHTENGILN
CCCCCCCCCCCCCCEEEEEHHHHCCCCCEEEECCCCHHHHHCCCCCCCEEEEEECCCEEE
FGAPPPAGQEDWDLINAGKKAVTLRPGAAFFHHADSFAMVRGGHLDVAILGAYQVSQNGD
CCCCCCCCCCCCHHHHCCCEEEEECCCCCEEECCCCEEEEECCCEEEEEEEEEEECCCCC
LANWRVGSKGVPAVGGAMDLVHGAKQVFVITEHQTKSGEPKLVESCAFPLTGVACVTRVY
CCEEEECCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHH
TSHAVVDIRGGRFILREKIPTLSLEELQAMTGARLHIDGPVADLVAPEL
HCEEEEEECCCEEEEECCCCCCCHHHHHHHHCCEEEECCCHHHHCCCCC
>Mature Secondary Structure 
TETLQNDGKLSNAQIAWRAAQDIADGAYVNLGIGFPEMVANYPVEGREVIFHTENGILN
CCCCCCCCCCCCCEEEEEHHHHCCCCCEEEECCCCHHHHHCCCCCCCEEEEEECCCEEE
FGAPPPAGQEDWDLINAGKKAVTLRPGAAFFHHADSFAMVRGGHLDVAILGAYQVSQNGD
CCCCCCCCCCCCHHHHCCCEEEEECCCCCEEECCCCEEEEECCCEEEEEEEEEEECCCCC
LANWRVGSKGVPAVGGAMDLVHGAKQVFVITEHQTKSGEPKLVESCAFPLTGVACVTRVY
CCEEEECCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHH
TSHAVVDIRGGRFILREKIPTLSLEELQAMTGARLHIDGPVADLVAPEL
HCEEEEEECCCEEEEECCCCCCCHHHHHHHHCCEEEECCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]