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The map label for this gene is meaA [H]

Identifier: 158422616

GI number: 158422616

Start: 1108477

End: 1110576

Strand: Reverse

Name: meaA [H]

Synonym: AZC_0992

Alternate gene names: 158422616

Gene position: 1110576-1108477 (Counterclockwise)

Preceding gene: 158422617

Following gene: 158422615

Centisome position: 20.68

GC content: 66.76

Gene sequence:

>2100_bases
ATGCGGCACAGTGCTCCGCGTCCCGCTTTCGCCCCTGCCCTACGCCTAAAATCGGCATCGACGCCGCCTACGTTGCGGCG
CAAGATATGTCGATCAGAACGTGCAGCAGCGCGAAGGGGAGGGATCATGACAAGCCGGGACAAACCTTGGCTGTTCCGCA
CCTATGCGGGTCACTCGACGGCGGCGGAATCGAACAAGCTCTACCGCGCCAACCTCGCCAAGGGGCAGACGGGCCTTTCG
GTAGCCTTCGATCTGCCCACCCAGACCGGCTATGACAGCGACCATCCCCTCGCACGCGGCGAAGTGGGCAAGGTGGGCGT
CCCCATTTCCCATCTCGGCGACATGCGCACCCTCTTCGAGGGTATTCCGCTGGCCGAGATGAACACCTCCATGACCATCA
ACGCCTGCGCCCCCTGGCTGCTGGCGCTCTATATCGCGGTGGCCGACGAGCAGGGCGCCGACCGGGCAAAGCTCCAGGGC
ACGACGCAGAACGACATCATCAAGGAATATCTCTCGCGCGGCACCTATGTGTTTCCGCCCGCGCCCTCCATGCGGCTCAC
CAAGGACGTGATCGTCTTCACGACCGAGCATCTGCCGCGCTGGAACCCCATGAACGTCTGCTCCTACCATCTGCAGGAGG
CCGGGGCGACGCCGGTGCAGGAGCTGGCCTTCGCGCTCGCGAACGCCATCGCCATCCTCGACACGGTGAAGAAGTCGGGC
GAGGCGGAAGGCGCGGCCTTTGGCGAGGTGGTGGGGCGCATCTCCTTCTTCGTGAATGCCGGCATGCGGTTCATTACCGA
AATGTGCAAGATGCGGGCGTTCGTGGACCTCTGGGACGAGATCTGCGTCAACCGCTACGGCATCACGGACGCCAAGCAGA
AGCTGTTCCGCTATGGCGTGCAGGTGAACTCGCTGGGCCTCACGGAGCAGCAGCCCGAGAACAACGTCTACCGCATCCTC
ATCGAGATGCTGGCGGTGACGCTCTCCAAGAAGGCCCGCGCCCGCGCCGTGCAGCTGCCGGCCTGGAACGAGGCCCTCGG
CCTGCCCCGCTCCTTCGACCAGCAATGGTCGCTGCGGATGCAGCAGGTGCTGGCCTATGAGACGGACCTGCTGGACTATG
GCGACATCTTCGACGGCTCGGTGGAGATCGACCGCAAGGTGGAAGCCCTGAAGGCCGAGGCGCGCGAGGAACTCGCCCGC
ATCGAGGCCATGGGCGGCGCGGTCGCGGCCATCGAGAACAGCTACATGAAGCAGCAGCTGGTGGAATCGAACACCCGCCG
GCTGGAAGCCATCGAGCGCGGCGAGCAGGTGGTCGTCGGCGTCAACCGCTGGACCGAGACCGAGCCCTCGCCACTGACCA
CAGGCGAGGGCGCCATCCTCACCGTGCCGGAGCATGTGGAGCCCGAGCAGGTGTCCCGTCTCAAGGCCTGGCGCGAGGCC
CGCGACCCGGCGGCGGTTGGGGCCGCTATGGACGCGCTGAAGCGTGCGGCGCGCGAAGGCGGCAACATCATGGAGCCCTC
CATCGCCTGCGCCAAGGCGGGCGTCACCACGGGCGAATGGGGCACCGCCCTGCGGGAGATCTTCGGCGAATATCGCGCGC
CCACCGGCGTCGGCCGCGCGGCGCGGGTGGACACCAAAGGCCTCGACGAAGTGCGCAGCAATGTCGAAGCGGTCTCGCAG
AAGCTCGGCCGCCGGCTCAAATTCCTCGTCGGCAAGCCCGGCCTCGACGGCCATTCCAACGGTGCCGAGCAGATCGCCGT
GCGCGCCCGCGATGCGGGCATGGAGGTGGTCTATGAAGGCATCCGCCTCACCCCGGCGGAGATCGTGAATGCCGCGCTGG
AGGAGAGCGTCCATGTGGTCGGCCTCTCCATCCTCTCCGGCTCCCACGTGCCGCTGGTGCGCGACGTGATGGAGCGGATG
CGTGCTGAAGGGCTCTCTGACGTTCCCGTCGTGGTGGGCGGCATCATCCCGCCGGAGGACGAGGCGCAGCTGCGCGGCTT
CGGGGTCGCGGCGGTCTATACGCCGAAGAATTTCGAGCTGAACCGCATCATGGCCGACATCGTCGCCATCGTGGACCGGG
AGGCCCAGGACGCGGCCTGA

Upstream 100 bases:

>100_bases
TCCGATTATCTGCCCGCCATGTTGCGCGTGCAGGAGGAGTTGCGGGTGCTCCTGAGCTGAACCCGTCACCCAAAGACCGG
CACCCCGGGCTCCTGCGGCA

Downstream 100 bases:

>100_bases
CCCGGCCGCCGGTCTCCGCTCGCGAACGAACCGCGTCCGGCGGCATTCCATCGATTCGGACGCGGTTTGATCCGCGCCTG
TTCCCGCGAGGGTGGCGGCC

Product: methylmalonyl-CoA mutase

Products: succinyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MRHSAPRPAFAPALRLKSASTPPTLRRKICRSERAAARRGGIMTSRDKPWLFRTYAGHSTAAESNKLYRANLAKGQTGLS
VAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFEGIPLAEMNTSMTINACAPWLLALYIAVADEQGADRAKLQG
TTQNDIIKEYLSRGTYVFPPAPSMRLTKDVIVFTTEHLPRWNPMNVCSYHLQEAGATPVQELAFALANAIAILDTVKKSG
EAEGAAFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEICVNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRIL
IEMLAVTLSKKARARAVQLPAWNEALGLPRSFDQQWSLRMQQVLAYETDLLDYGDIFDGSVEIDRKVEALKAEAREELAR
IEAMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVNRWTETEPSPLTTGEGAILTVPEHVEPEQVSRLKAWREA
RDPAAVGAAMDALKRAAREGGNIMEPSIACAKAGVTTGEWGTALREIFGEYRAPTGVGRAARVDTKGLDEVRSNVEAVSQ
KLGRRLKFLVGKPGLDGHSNGAEQIAVRARDAGMEVVYEGIRLTPAEIVNAALEESVHVVGLSILSGSHVPLVRDVMERM
RAEGLSDVPVVVGGIIPPEDEAQLRGFGVAAVYTPKNFELNRIMADIVAIVDREAQDAA

Sequences:

>Translated_699_residues
MRHSAPRPAFAPALRLKSASTPPTLRRKICRSERAAARRGGIMTSRDKPWLFRTYAGHSTAAESNKLYRANLAKGQTGLS
VAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFEGIPLAEMNTSMTINACAPWLLALYIAVADEQGADRAKLQG
TTQNDIIKEYLSRGTYVFPPAPSMRLTKDVIVFTTEHLPRWNPMNVCSYHLQEAGATPVQELAFALANAIAILDTVKKSG
EAEGAAFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEICVNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRIL
IEMLAVTLSKKARARAVQLPAWNEALGLPRSFDQQWSLRMQQVLAYETDLLDYGDIFDGSVEIDRKVEALKAEAREELAR
IEAMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVNRWTETEPSPLTTGEGAILTVPEHVEPEQVSRLKAWREA
RDPAAVGAAMDALKRAAREGGNIMEPSIACAKAGVTTGEWGTALREIFGEYRAPTGVGRAARVDTKGLDEVRSNVEAVSQ
KLGRRLKFLVGKPGLDGHSNGAEQIAVRARDAGMEVVYEGIRLTPAEIVNAALEESVHVVGLSILSGSHVPLVRDVMERM
RAEGLSDVPVVVGGIIPPEDEAQLRGFGVAAVYTPKNFELNRIMADIVAIVDREAQDAA
>Mature_699_residues
MRHSAPRPAFAPALRLKSASTPPTLRRKICRSERAAARRGGIMTSRDKPWLFRTYAGHSTAAESNKLYRANLAKGQTGLS
VAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFEGIPLAEMNTSMTINACAPWLLALYIAVADEQGADRAKLQG
TTQNDIIKEYLSRGTYVFPPAPSMRLTKDVIVFTTEHLPRWNPMNVCSYHLQEAGATPVQELAFALANAIAILDTVKKSG
EAEGAAFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEICVNRYGITDAKQKLFRYGVQVNSLGLTEQQPENNVYRIL
IEMLAVTLSKKARARAVQLPAWNEALGLPRSFDQQWSLRMQQVLAYETDLLDYGDIFDGSVEIDRKVEALKAEAREELAR
IEAMGGAVAAIENSYMKQQLVESNTRRLEAIERGEQVVVGVNRWTETEPSPLTTGEGAILTVPEHVEPEQVSRLKAWREA
RDPAAVGAAMDALKRAAREGGNIMEPSIACAKAGVTTGEWGTALREIFGEYRAPTGVGRAARVDTKGLDEVRSNVEAVSQ
KLGRRLKFLVGKPGLDGHSNGAEQIAVRARDAGMEVVYEGIRLTPAEIVNAALEESVHVVGLSILSGSHVPLVRDVMERM
RAEGLSDVPVVVGGIIPPEDEAQLRGFGVAAVYTPKNFELNRIMADIVAIVDREAQDAA

Specific function: Required for the assimilation of methanol and ethanol [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=634, Percent_Identity=35.6466876971609, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI1789284, Length=645, Percent_Identity=39.3798449612403, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25144500, Length=633, Percent_Identity=34.913112164297, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: 5.4.99.2

Molecular weight: Translated: 76418; Mature: 76418

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSAPRPAFAPALRLKSASTPPTLRRKICRSERAAARRGGIMTSRDKPWLFRTYAGHST
CCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
AAESNKLYRANLAKGQTGLSVAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFE
CCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GIPLAEMNTSMTINACAPWLLALYIAVADEQGADRAKLQGTTQNDIIKEYLSRGTYVFPP
CCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHCCCEEECC
APSMRLTKDVIVFTTEHLPRWNPMNVCSYHLQEAGATPVQELAFALANAIAILDTVKKSG
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EAEGAAFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEICVNRYGITDAKQKLFRYGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
QVNSLGLTEQQPENNVYRILIEMLAVTLSKKARARAVQLPAWNEALGLPRSFDQQWSLRM
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHCCCCCCCHHHHHHH
QQVLAYETDLLDYGDIFDGSVEIDRKVEALKAEAREELARIEAMGGAVAAIENSYMKQQL
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VESNTRRLEAIERGEQVVVGVNRWTETEPSPLTTGEGAILTVPEHVEPEQVSRLKAWREA
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
RDPAAVGAAMDALKRAAREGGNIMEPSIACAKAGVTTGEWGTALREIFGEYRAPTGVGRA
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
ARVDTKGLDEVRSNVEAVSQKLGRRLKFLVGKPGLDGHSNGAEQIAVRARDAGMEVVYEG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEHHHHCCHHHHHHC
IRLTPAEIVNAALEESVHVVGLSILSGSHVPLVRDVMERMRAEGLSDVPVVVGGIIPPED
CCCCHHHHHHHHHHCCHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
EAQLRGFGVAAVYTPKNFELNRIMADIVAIVDREAQDAA
CHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MRHSAPRPAFAPALRLKSASTPPTLRRKICRSERAAARRGGIMTSRDKPWLFRTYAGHST
CCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCC
AAESNKLYRANLAKGQTGLSVAFDLPTQTGYDSDHPLARGEVGKVGVPISHLGDMRTLFE
CCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
GIPLAEMNTSMTINACAPWLLALYIAVADEQGADRAKLQGTTQNDIIKEYLSRGTYVFPP
CCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHCCCEEECC
APSMRLTKDVIVFTTEHLPRWNPMNVCSYHLQEAGATPVQELAFALANAIAILDTVKKSG
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EAEGAAFGEVVGRISFFVNAGMRFITEMCKMRAFVDLWDEICVNRYGITDAKQKLFRYGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
QVNSLGLTEQQPENNVYRILIEMLAVTLSKKARARAVQLPAWNEALGLPRSFDQQWSLRM
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHCCCCCCCHHHHHHH
QQVLAYETDLLDYGDIFDGSVEIDRKVEALKAEAREELARIEAMGGAVAAIENSYMKQQL
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VESNTRRLEAIERGEQVVVGVNRWTETEPSPLTTGEGAILTVPEHVEPEQVSRLKAWREA
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
RDPAAVGAAMDALKRAAREGGNIMEPSIACAKAGVTTGEWGTALREIFGEYRAPTGVGRA
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
ARVDTKGLDEVRSNVEAVSQKLGRRLKFLVGKPGLDGHSNGAEQIAVRARDAGMEVVYEG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEHHHHCCHHHHHHC
IRLTPAEIVNAALEESVHVVGLSILSGSHVPLVRDVMERMRAEGLSDVPVVVGGIIPPED
CCCCHHHHHHHHHHCCHHEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCC
EAQLRGFGVAAVYTPKNFELNRIMADIVAIVDREAQDAA
CHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-methylmalonyl-CoA

Specific reaction: (R)-methylmalonyl-CoA = succinyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]