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The map label for this gene is proP [H]

Identifier: 158422501

GI number: 158422501

Start: 977638

End: 979305

Strand: Reverse

Name: proP [H]

Synonym: AZC_0877

Alternate gene names: 158422501

Gene position: 979305-977638 (Counterclockwise)

Preceding gene: 158422502

Following gene: 158422499

Centisome position: 18.24

GC content: 65.71

Gene sequence:

>1668_bases
ATGTCTGTCACGACTGCTGGCCCGGCCACGGCCCATGCACCGATGACGCGGGAGGAGAAGAAGGTCATCTTCGCCTCGTC
CCTCGGCACGGTGTTCGAGTGGTACGATTTCTATCTCTACGGATCGCTTGCCGCGATCATCGGCGCGCAATTCTTCAGCG
CCTATCCGCCGGCGACCCGCGATATCTTCGCGCTGCTGGCCTTCGCCGCCGGCTTCCTCGTGCGTCCCTTCGGCGCCATC
GTGTTCGGGCGCATCGGCGATCTCGTCGGCCGCAAGTACACCTTCCTCGTCACCATCCTCATCATGGGCCTGTCGACCTT
CATCGTCGGCATCCTGCCCAATGCGGCCACCATCGGCATCGCGGCTCCGATCATCCTGATCGTCCTGCGCCTGCTGCAGG
GCCTCGCGCTCGGCGGCGAGTATGGCGGCGCGGCCACCTACGTGGCGGAGCATGCGCCGCACGGCCGCCGCGGCTTCTAC
ACCTCCTGGATCCAGACCACCGCGACCCTGGGCCTGTTCCTGTCGCTGCTGGTGATCCTGTTCACCCGCTCCATCGTGGG
CGAGCAGAACTTCGCCGCCTGGGGCTGGCGCATTCCCTTCCTGGTGTCCATCGCACTGCTCGGCATCTCGGTGTGGATCC
GCTTCCAGCTCAATGAATCCCCGGCCTTCAAGAAGATGAAGGAAGAGGGCACGACCTCCAAGGCGCCGCTCTCGGAAGCC
TTCGGCCAGTGGAAGAACCTGAAGTTCGTGCTGCTCGCCCTCTTCGGCCTCGTCGCCGGCCAGGGTGTGGTCTGGTACAC
GGGCCAGTTCTATGCCCTGTTCTTCCTGCAATCGATCCTGAAGGTGGATGGCTACACCTCCAACCTGCTGATCGCGTGGT
CGCTGCTGCTGGGCACGGGCTTCTTCGTGTTCTTCGGCTGGCTGTCGGACAAGATCGGCCGCAAGCCCATCATCCTTGCC
GGCTGCCTCATCGCCGCCGCCAGCTACTTCCCGCTCTTCAACGCCATCACCTCCTACGCCAACCCGGCGCTGGAGAATGC
GATCCAGAACGTGAAGGTCACCGTCGTCGCCTCGCCCGGCGAATGCGGCAGCCTCTTCAACCCGGTCGGCACCCGCGTCT
ACTCGGCGCCGTGCGACGTGGCCCGCGACTTCCTCGCCAAGTCCTCGGTGAAGTATTCCACCGAGACCGGCGCGGCGGGC
TCCGGCACCGTGCTGAAGGTCAACGACACCGTGGTGCCCTTCGGCCCCGACTTCGCCAAGACCGCCACGGCGGCGCTCCA
GGCGGCGGGCTACCCGAGCGCGGCGACCAACAAGGACGTGATCAAGATGTCGCATCCGTTCGACATCTTCCAGCCGCGGG
TCGCGGCGGTCATCGGCCTGCTGTTCGTCCTCGTGCTCTTCGTGACCATGGTGTACGGCCCCATCGCCGCCGCGCTGGTG
GAACTCTTCCCCACCCGCGTCCGCTACACCTCCATGTCGCTGCCCTACCATATCGGCAACGGCTGGTTCGGCGGCCTGCT
GCCGGCCACCGCCTTCGCCATGGTGGCGGCCACCGGCGACATCTACTACGGCCTCTGGTACCCGATCGTGATCGCGCTCG
GCACCTTCGTCATCGGCCTGCTGTTCGTGCCGGAGACCAAGGACCGGGACATCGACGACTGGCACTGA

Upstream 100 bases:

>100_bases
GCGGACGGTTGCTCGCAGGTGCCTCAGCTCCCGGGCGCGCCCGCCTCCGCCTTCCGGCCCCATTGGGCCGCCGAGGCACC
CGCAACTGGGAGGAGGAACA

Downstream 100 bases:

>100_bases
GCGGGTTTGCCCCGGCCGCGCGCCGGCCGGGGCTTCCATCCTTCAAAAAGCAACGGCCTCGCGTCACCGCGAGGCCGTTC
GCTTTTCAGCCCTCCCCTTC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MSVTTAGPATAHAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAI
VFGRIGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGIAAPIILIVLRLLQGLALGGEYGGAATYVAEHAPHGRRGFY
TSWIQTTATLGLFLSLLVILFTRSIVGEQNFAAWGWRIPFLVSIALLGISVWIRFQLNESPAFKKMKEEGTTSKAPLSEA
FGQWKNLKFVLLALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGFFVFFGWLSDKIGRKPIILA
GCLIAAASYFPLFNAITSYANPALENAIQNVKVTVVASPGECGSLFNPVGTRVYSAPCDVARDFLAKSSVKYSTETGAAG
SGTVLKVNDTVVPFGPDFAKTATAALQAAGYPSAATNKDVIKMSHPFDIFQPRVAAVIGLLFVLVLFVTMVYGPIAAALV
ELFPTRVRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALGTFVIGLLFVPETKDRDIDDWH

Sequences:

>Translated_555_residues
MSVTTAGPATAHAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAI
VFGRIGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGIAAPIILIVLRLLQGLALGGEYGGAATYVAEHAPHGRRGFY
TSWIQTTATLGLFLSLLVILFTRSIVGEQNFAAWGWRIPFLVSIALLGISVWIRFQLNESPAFKKMKEEGTTSKAPLSEA
FGQWKNLKFVLLALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGFFVFFGWLSDKIGRKPIILA
GCLIAAASYFPLFNAITSYANPALENAIQNVKVTVVASPGECGSLFNPVGTRVYSAPCDVARDFLAKSSVKYSTETGAAG
SGTVLKVNDTVVPFGPDFAKTATAALQAAGYPSAATNKDVIKMSHPFDIFQPRVAAVIGLLFVLVLFVTMVYGPIAAALV
ELFPTRVRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALGTFVIGLLFVPETKDRDIDDWH
>Mature_554_residues
SVTTAGPATAHAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIV
FGRIGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGIAAPIILIVLRLLQGLALGGEYGGAATYVAEHAPHGRRGFYT
SWIQTTATLGLFLSLLVILFTRSIVGEQNFAAWGWRIPFLVSIALLGISVWIRFQLNESPAFKKMKEEGTTSKAPLSEAF
GQWKNLKFVLLALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTGFFVFFGWLSDKIGRKPIILAG
CLIAAASYFPLFNAITSYANPALENAIQNVKVTVVASPGECGSLFNPVGTRVYSAPCDVARDFLAKSSVKYSTETGAAGS
GTVLKVNDTVVPFGPDFAKTATAALQAAGYPSAATNKDVIKMSHPFDIFQPRVAAVIGLLFVLVLFVTMVYGPIAAALVE
LFPTRVRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALGTFVIGLLFVPETKDRDIDDWH

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=332, Percent_Identity=37.0481927710843, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788292, Length=335, Percent_Identity=34.0298507462687, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788942, Length=335, Percent_Identity=33.4328358208955, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789941, Length=349, Percent_Identity=34.9570200573066, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082404, Length=329, Percent_Identity=24.9240121580547, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082231, Length=242, Percent_Identity=26.4462809917355, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59909; Mature: 59777

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTTAGPATAHAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATR
CCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DIFALLAFAAGFLVRPFGAIVFGRIGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AAPIILIVLRLLQGLALGGEYGGAATYVAEHAPHGRRGFYTSWIQTTATLGLFLSLLVIL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FTRSIVGEQNFAAWGWRIPFLVSIALLGISVWIRFQLNESPAFKKMKEEGTTSKAPLSEA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCHHHH
FGQWKNLKFVLLALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTG
HHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FFVFFGWLSDKIGRKPIILAGCLIAAASYFPLFNAITSYANPALENAIQNVKVTVVASPG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECCC
ECGSLFNPVGTRVYSAPCDVARDFLAKSSVKYSTETGAAGSGTVLKVNDTVVPFGPDFAK
CCCHHHCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEECCCCCHHH
TATAALQAAGYPSAATNKDVIKMSHPFDIFQPRVAAVIGLLFVLVLFVTMVYGPIAAALV
HHHHHHHHCCCCCCCCCCCEEEECCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFPTRVRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALGTFVIGL
HHHHHHHEEEEECCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LFVPETKDRDIDDWH
HCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVTTAGPATAHAPMTREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFSAYPPATR
CCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
DIFALLAFAAGFLVRPFGAIVFGRIGDLVGRKYTFLVTILIMGLSTFIVGILPNAATIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AAPIILIVLRLLQGLALGGEYGGAATYVAEHAPHGRRGFYTSWIQTTATLGLFLSLLVIL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FTRSIVGEQNFAAWGWRIPFLVSIALLGISVWIRFQLNESPAFKKMKEEGTTSKAPLSEA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHCCCCCCCCHHHH
FGQWKNLKFVLLALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLLLGTG
HHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FFVFFGWLSDKIGRKPIILAGCLIAAASYFPLFNAITSYANPALENAIQNVKVTVVASPG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECCC
ECGSLFNPVGTRVYSAPCDVARDFLAKSSVKYSTETGAAGSGTVLKVNDTVVPFGPDFAK
CCCHHHCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCEEECCCCCHHH
TATAALQAAGYPSAATNKDVIKMSHPFDIFQPRVAAVIGLLFVLVLFVTMVYGPIAAALV
HHHHHHHHCCCCCCCCCCCEEEECCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELFPTRVRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALGTFVIGL
HHHHHHHEEEEECCCEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LFVPETKDRDIDDWH
HCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]