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The map label for this gene is trmH [H]

Identifier: 158422502

GI number: 158422502

Start: 979699

End: 980544

Strand: Reverse

Name: trmH [H]

Synonym: AZC_0878

Alternate gene names: 158422502

Gene position: 980544-979699 (Counterclockwise)

Preceding gene: 158422503

Following gene: 158422501

Centisome position: 18.26

GC content: 71.87

Gene sequence:

>846_bases
ATGTCCGATCGTCCCGAACGCCCGCCCTTCCGCTCCGCCGGCCGCCCCTTCGCGGCCAAAGGCGGTGCCCGCCCCTTTCC
GCCCAAGCGCAAGGGCGGCCCCGGCCGCCCGCCGGCCTGGGAGGGCGAGAATGACGTGGCCCTGCTCTATGGCTGGCATA
CGGTGAGCGAGGCGCTGGCCAATCCGGCGCGGCGCTTCCGCAAGATCCTCGCCACGGAGAACGCCGCCAACCGCCTGCAG
GAGGAGGGCTTCCCCCTCCCCGTCGCGCCGGAGATCGTGCGCCCTTCCGAGATCGACAAGCTGCTCGGTTCGGACGCGGT
GCATCAGGGCCTGCTGGCGGAGGCCGAACACCTGCCCTCCCCGCCCCTCAAGAAGGTGGCGAAGTCGGACCTCGTTCTGG
TGCTCGACCAGATCACCGATCCGCACAATGTGGGCGCCATCGTGCGCTCCGCCGCCGCCTTTGCGGTGGGCGCCATCGTC
ACCACGGCCCGCCACAGCCCCGTCGCCACCGGCGTGCTGGCGAAAAGCGCCTCCGGCGGGCTGGAACACGTGCCCTTCTG
CCTCGTGACCAACCTCGCCCGGGCGCTCGAGGAACTGAAGGCCTCCGGCATGCTCGTGGTGGGCCTCGACAGCGAAGGTC
CGGCCGATCTCGAGGACACGGCCATGCGCCCGCCGATGGCACTGGTGCTGGGCGCGGAAGGCAAGGGCCTGCGGCAGCTG
ACGCGGGAGACCTGCGACGTGCTCGCCCGCATCGACCTGCCCGGCGCCATCAAGAGCCTCAACGTCTCCAATGCCGCCGC
CCTGTCGCTCGGCATCGCCCGCCGCGCCCTGAAGCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
ACCGATGCTCGCCGCCAAGGGGATGGAGAAGCCGTAAGCGAAAGCGGCCGGGCGCGGACGGCCCCTTGCGCCGCCGCGCC
GGCTCTGACAAAGCCTTCCC

Downstream 100 bases:

>100_bases
GCAGGACACCAACAACGGCTGGCGCCGGTCGCGCCGGCCGGTTCGTTTGCAAGCTAATAATCTCAAATAATGCGCGCGCC
CCTTTGCGCGGCTAAACACG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSDRPERPPFRSAGRPFAAKGGARPFPPKRKGGPGRPPAWEGENDVALLYGWHTVSEALANPARRFRKILATENAANRLQ
EEGFPLPVAPEIVRPSEIDKLLGSDAVHQGLLAEAEHLPSPPLKKVAKSDLVLVLDQITDPHNVGAIVRSAAAFAVGAIV
TTARHSPVATGVLAKSASGGLEHVPFCLVTNLARALEELKASGMLVVGLDSEGPADLEDTAMRPPMALVLGAEGKGLRQL
TRETCDVLARIDLPGAIKSLNVSNAAALSLGIARRALKRAG

Sequences:

>Translated_281_residues
MSDRPERPPFRSAGRPFAAKGGARPFPPKRKGGPGRPPAWEGENDVALLYGWHTVSEALANPARRFRKILATENAANRLQ
EEGFPLPVAPEIVRPSEIDKLLGSDAVHQGLLAEAEHLPSPPLKKVAKSDLVLVLDQITDPHNVGAIVRSAAAFAVGAIV
TTARHSPVATGVLAKSASGGLEHVPFCLVTNLARALEELKASGMLVVGLDSEGPADLEDTAMRPPMALVLGAEGKGLRQL
TRETCDVLARIDLPGAIKSLNVSNAAALSLGIARRALKRAG
>Mature_280_residues
SDRPERPPFRSAGRPFAAKGGARPFPPKRKGGPGRPPAWEGENDVALLYGWHTVSEALANPARRFRKILATENAANRLQE
EGFPLPVAPEIVRPSEIDKLLGSDAVHQGLLAEAEHLPSPPLKKVAKSDLVLVLDQITDPHNVGAIVRSAAAFAVGAIVT
TARHSPVATGVLAKSASGGLEHVPFCLVTNLARALEELKASGMLVVGLDSEGPADLEDTAMRPPMALVLGAEGKGLRQLT
RETCDVLARIDLPGAIKSLNVSNAAALSLGIARRALKRAG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=186, Percent_Identity=34.4086021505376, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=33.0578512396694, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1788935, Length=151, Percent_Identity=35.0993377483444, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790083, Length=153, Percent_Identity=30.718954248366, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=255, Percent_Identity=28.2352941176471, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=285, Percent_Identity=28.4210526315789, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29562; Mature: 29431

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRPERPPFRSAGRPFAAKGGARPFPPKRKGGPGRPPAWEGENDVALLYGWHTVSEALA
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHH
NPARRFRKILATENAANRLQEEGFPLPVAPEIVRPSEIDKLLGSDAVHQGLLAEAEHLPS
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
PPLKKVAKSDLVLVLDQITDPHNVGAIVRSAAAFAVGAIVTTARHSPVATGVLAKSASGG
CHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
LEHVPFCLVTNLARALEELKASGMLVVGLDSEGPADLEDTAMRPPMALVLGAEGKGLRQL
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHCCCCEEEEECCCCCHHHHH
TRETCDVLARIDLPGAIKSLNVSNAAALSLGIARRALKRAG
HHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDRPERPPFRSAGRPFAAKGGARPFPPKRKGGPGRPPAWEGENDVALLYGWHTVSEALA
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHH
NPARRFRKILATENAANRLQEEGFPLPVAPEIVRPSEIDKLLGSDAVHQGLLAEAEHLPS
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCC
PPLKKVAKSDLVLVLDQITDPHNVGAIVRSAAAFAVGAIVTTARHSPVATGVLAKSASGG
CHHHHHHHCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
LEHVPFCLVTNLARALEELKASGMLVVGLDSEGPADLEDTAMRPPMALVLGAEGKGLRQL
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHCCCCEEEEECCCCCHHHHH
TRETCDVLARIDLPGAIKSLNVSNAAALSLGIARRALKRAG
HHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]