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The map label for this gene is fis [H]

Identifier: 158422383

GI number: 158422383

Start: 857627

End: 859711

Strand: Reverse

Name: fis [H]

Synonym: AZC_0759

Alternate gene names: 158422383

Gene position: 859711-857627 (Counterclockwise)

Preceding gene: 158422388

Following gene: 158422381

Centisome position: 16.01

GC content: 68.35

Gene sequence:

>2085_bases
ATGCCGGAGTGGGTCCTTACGCTAAGCAAGCCACGTGCCAGCGCCCACCGAGCCGTGCGATCAAGGACGGCGAAGGATTT
CACGCCAGCGACTGGATTTACGAATTTTCGTCATTTACGATACTTCAGATCCCACTGGCGAAAATTCGTAATGACGCTCC
CGCAGGCCGCACCGCTCGCCGCGCAGTTGCCGGAGACAGCAACGCCGCCCTCGCCTTTCGGCCCGGCCTTCCTGCACGGC
GCCGAACCGGCGTTGGTGGTCGATCTGGAGACCGCGCGCATCCGGGACGCCAATGCCGCCGCATGTCGGCTGCTGGGCCA
CTGGCGCCTCGCACTGCAAGGGATGACTGTGTTCGACCTGCATCCGCAGCAGACCGGGCCGCTCATCGTCTTCACGCAGG
CGGTGCTGGAACGGGGCAGCTTCTGGACCCGCACGCTCCAGCCCCAGCACGCCGACGGCCGGCAGCTGGACGTAGAGTGT
GCCGGCACGCTCATGCCCACCGCCGAGGGCCGGCTGCTCCTGCTCACCCTCTGCGATCTCGACGCCCGTCACCGGCGCGA
TCTAGAGGCGCGGGCCGAGGCCTTCATGCACGAAGGTCTTGCGGAATGGCAGCGGGCCGACCGGCTGTTCGCGGACATCG
AGCGCGAGAACCGGCTCATTTTGAGGGCGGTGGGCGAAGGCATCTATGGGGTGAATGCGGAAGGCCGCACCACCTTCGTC
AATCCCGCCGCCGAGCAGATCCTCGGCTGGACGGCAGAGGAGATGATCGGCCGCGACATGCACGCCATGGTGCATCACCA
TCATCACGACGGCTCGGCCTATCCGAACACCACCTGCCCCATCTATGCAGCCTTCCGCGATGGCGCCGTCCACCATGTGG
AGAACGAGGTGTTCTGGCATCGCTCCGGCCGGCCGGTGTGGGTGGAATACACCTCCACCCCGCTGCGCGACCGGGGCCGC
CTCATCGGCGCCGTGGTGGTGTTCCGCGATGTGACGCAGAAGCGGGAGGCCAACGAGCAGCTTCGGGCCGCGCTGGCCGA
GGTGGACCGCCTGCGCGAGCGGCTGGAGATGGAGAATGCCTACCTTCAGGAGGAAATCCGCGCCGAGACCAGCCATCGCG
GCATCATCGGCCGCTCGGAGGCCATCAAGAAGGTGCTGAGGCAGGTGGACCTTGTCGCCCCGACCGACGCCACCGTCCTC
ATCACGGGCGAGAGCGGCACCGGCAAGGAGCTGATCGCCCGCGCCATCCACGATGCATCGGAGCGACGGGAGCGCCCGCT
CATCCGCGTCAATTGCGCGGCCATCCCGCGCGAGCTGTTCGAGAGCGAATTCTTCGGCCATGTGCGCGGCGCCTTCACCG
GTGCCCTGCGCGATCGCGTGGGCCGGTTCGAGCTGGCGGACCGGGGCACGCTCTTCCTCGACGAGGTGGGCGAGATCCCG
CTGGAGCTCCAGTCGAAACTGCTGCGCGTGCTTCAGGAGGGGCAGTTCGAGCGCATCGGCGAGGAACGCACGCGGCAGGT
GGATGTGCGCATCATCGCCGCCACCAATCGCGACCTTAAGGCCGCCGTGCGGGCCGGGCGCTTCCGGGAGGACCTGTTCT
ACCGGCTCGACGTCATGCCCATCGAATCCGTGCCCCTGCGCGAGCGACGAGAGGACATCCCGCTCCTTGCGGCGCATTTC
CTCGACGGCGTGCGGCGCAAGCCGGCCGATGGACCGCTCCAGCTCAGCGCCGGCGACATGCGCCGCCTCCAGACCTATGA
CTGGCCCGGCAATGTCCGCGAGCTTCAGAACGTGATCGAGCGCGGCATCATCCTCGCGCGCAATGGCCGCCTGCACATCG
ATCTTCCGGACCGGGAGGCCCCCGCCACCGCGCAGCCGGTACCGCCTTCCGCCGAACTGGAAACCGAGGCCGATCGTCGC
CTGCGGCTGCGGACCAGCATCCTCGCGGCGCTGGAGGCGGCGGGCGGCAAGGTCTCCGGTCCCGGCGGGGCTGCCGAACG
CCTCGGCCTCAAGCCGACGACCCTCGCCTCCCGCATGAAGACGCTCGGGATCACGCCGGGGCGTGCCTCACGCTCCGGCG
CCTGA

Upstream 100 bases:

>100_bases
CCGCGGAGCGATGCCGGCCGCAGGAGCAGCCGGATAACAAGCGTGAAAACCGCCGCACAGGATCAAAGCGCGCCATGAAC
CGCCTCGCTCGCAGAAGGAG

Downstream 100 bases:

>100_bases
AACGGCCGCGGACGCTCACCCTCCGGCAGCGATCCGTCGGCAGTGGTTGAGCGCGGCTTCGATGAGGTTGTCGCTGGCCT
CCGGCGTGCGGAAGGCGGAA

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MPEWVLTLSKPRASAHRAVRSRTAKDFTPATGFTNFRHLRYFRSHWRKFVMTLPQAAPLAAQLPETATPPSPFGPAFLHG
AEPALVVDLETARIRDANAAACRLLGHWRLALQGMTVFDLHPQQTGPLIVFTQAVLERGSFWTRTLQPQHADGRQLDVEC
AGTLMPTAEGRLLLLTLCDLDARHRRDLEARAEAFMHEGLAEWQRADRLFADIERENRLILRAVGEGIYGVNAEGRTTFV
NPAAEQILGWTAEEMIGRDMHAMVHHHHHDGSAYPNTTCPIYAAFRDGAVHHVENEVFWHRSGRPVWVEYTSTPLRDRGR
LIGAVVVFRDVTQKREANEQLRAALAEVDRLRERLEMENAYLQEEIRAETSHRGIIGRSEAIKKVLRQVDLVAPTDATVL
ITGESGTGKELIARAIHDASERRERPLIRVNCAAIPRELFESEFFGHVRGAFTGALRDRVGRFELADRGTLFLDEVGEIP
LELQSKLLRVLQEGQFERIGEERTRQVDVRIIAATNRDLKAAVRAGRFREDLFYRLDVMPIESVPLRERREDIPLLAAHF
LDGVRRKPADGPLQLSAGDMRRLQTYDWPGNVRELQNVIERGIILARNGRLHIDLPDREAPATAQPVPPSAELETEADRR
LRLRTSILAALEAAGGKVSGPGGAAERLGLKPTTLASRMKTLGITPGRASRSGA

Sequences:

>Translated_694_residues
MPEWVLTLSKPRASAHRAVRSRTAKDFTPATGFTNFRHLRYFRSHWRKFVMTLPQAAPLAAQLPETATPPSPFGPAFLHG
AEPALVVDLETARIRDANAAACRLLGHWRLALQGMTVFDLHPQQTGPLIVFTQAVLERGSFWTRTLQPQHADGRQLDVEC
AGTLMPTAEGRLLLLTLCDLDARHRRDLEARAEAFMHEGLAEWQRADRLFADIERENRLILRAVGEGIYGVNAEGRTTFV
NPAAEQILGWTAEEMIGRDMHAMVHHHHHDGSAYPNTTCPIYAAFRDGAVHHVENEVFWHRSGRPVWVEYTSTPLRDRGR
LIGAVVVFRDVTQKREANEQLRAALAEVDRLRERLEMENAYLQEEIRAETSHRGIIGRSEAIKKVLRQVDLVAPTDATVL
ITGESGTGKELIARAIHDASERRERPLIRVNCAAIPRELFESEFFGHVRGAFTGALRDRVGRFELADRGTLFLDEVGEIP
LELQSKLLRVLQEGQFERIGEERTRQVDVRIIAATNRDLKAAVRAGRFREDLFYRLDVMPIESVPLRERREDIPLLAAHF
LDGVRRKPADGPLQLSAGDMRRLQTYDWPGNVRELQNVIERGIILARNGRLHIDLPDREAPATAQPVPPSAELETEADRR
LRLRTSILAALEAAGGKVSGPGGAAERLGLKPTTLASRMKTLGITPGRASRSGA
>Mature_693_residues
PEWVLTLSKPRASAHRAVRSRTAKDFTPATGFTNFRHLRYFRSHWRKFVMTLPQAAPLAAQLPETATPPSPFGPAFLHGA
EPALVVDLETARIRDANAAACRLLGHWRLALQGMTVFDLHPQQTGPLIVFTQAVLERGSFWTRTLQPQHADGRQLDVECA
GTLMPTAEGRLLLLTLCDLDARHRRDLEARAEAFMHEGLAEWQRADRLFADIERENRLILRAVGEGIYGVNAEGRTTFVN
PAAEQILGWTAEEMIGRDMHAMVHHHHHDGSAYPNTTCPIYAAFRDGAVHHVENEVFWHRSGRPVWVEYTSTPLRDRGRL
IGAVVVFRDVTQKREANEQLRAALAEVDRLRERLEMENAYLQEEIRAETSHRGIIGRSEAIKKVLRQVDLVAPTDATVLI
TGESGTGKELIARAIHDASERRERPLIRVNCAAIPRELFESEFFGHVRGAFTGALRDRVGRFELADRGTLFLDEVGEIPL
ELQSKLLRVLQEGQFERIGEERTRQVDVRIIAATNRDLKAAVRAGRFREDLFYRLDVMPIESVPLRERREDIPLLAAHFL
DGVRRKPADGPLQLSAGDMRRLQTYDWPGNVRELQNVIERGIILARNGRLHIDLPDREAPATAQPVPPSAELETEADRRL
RLRTSILAALEAAGGKVSGPGGAAERLGLKPTTLASRMKTLGITPGRASRSGA

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=54.6783625730994, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI87082117, Length=348, Percent_Identity=50.5747126436782, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI1790437, Length=349, Percent_Identity=45.5587392550143, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=54.2372881355932, Blast_Score=265, Evalue=1e-71,
Organism=Escherichia coli, GI1788550, Length=323, Percent_Identity=47.0588235294118, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=51.7241379310345, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=43.9759036144578, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1789233, Length=238, Percent_Identity=45.7983193277311, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI87081872, Length=234, Percent_Identity=47.8632478632479, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786524, Length=321, Percent_Identity=40.809968847352, Blast_Score=196, Evalue=6e-51,
Organism=Escherichia coli, GI1787583, Length=472, Percent_Identity=31.1440677966102, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081858, Length=294, Percent_Identity=35.0340136054422, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1789828, Length=265, Percent_Identity=33.9622641509434, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1788381, Length=140, Percent_Identity=34.2857142857143, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 77755; Mature: 77624

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEWVLTLSKPRASAHRAVRSRTAKDFTPATGFTNFRHLRYFRSHWRKFVMTLPQAAPLA
CCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
AQLPETATPPSPFGPAFLHGAEPALVVDLETARIRDANAAACRLLGHWRLALQGMTVFDL
HHCCCCCCCCCCCCCHHHCCCCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHCCCEEEEC
HPQQTGPLIVFTQAVLERGSFWTRTLQPQHADGRQLDVECAGTLMPTAEGRLLLLTLCDL
CCCCCCCEEEEEHHHHHCCCCEEECCCCCCCCCCEEEEEECCEECCCCCCCEEEEEECCC
DARHRRDLEARAEAFMHEGLAEWQRADRLFADIERENRLILRAVGEGIYGVNAEGRTTFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCEEEE
NPAAEQILGWTAEEMIGRDMHAMVHHHHHDGSAYPNTTCPIYAAFRDGAVHHVENEVFWH
CHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHCCCCEEEE
RSGRPVWVEYTSTPLRDRGRLIGAVVVFRDVTQKREANEQLRAALAEVDRLRERLEMENA
CCCCEEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQEEIRAETSHRGIIGRSEAIKKVLRQVDLVAPTDATVLITGESGTGKELIARAIHDAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
ERRERPLIRVNCAAIPRELFESEFFGHVRGAFTGALRDRVGRFELADRGTLFLDEVGEIP
HHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEHHHCCCC
LELQSKLLRVLQEGQFERIGEERTRQVDVRIIAATNRDLKAAVRAGRFREDLFYRLDVMP
HHHHHHHHHHHHCCCHHHHHHHHCEEEEEEEEEECCCHHHHHHHHCCHHHHHHHEEECCC
IESVPLRERREDIPLLAAHFLDGVRRKPADGPLQLSAGDMRRLQTYDWPGNVRELQNVIE
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHCCCCCCCCHHHHHHHHH
RGIILARNGRLHIDLPDREAPATAQPVPPSAELETEADRRLRLRTSILAALEAAGGKVSG
CCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PGGAAERLGLKPTTLASRMKTLGITPGRASRSGA
CCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PEWVLTLSKPRASAHRAVRSRTAKDFTPATGFTNFRHLRYFRSHWRKFVMTLPQAAPLA
CCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
AQLPETATPPSPFGPAFLHGAEPALVVDLETARIRDANAAACRLLGHWRLALQGMTVFDL
HHCCCCCCCCCCCCCHHHCCCCCEEEEEECHHHCCCCCHHHHHHHHHHHHHHCCCEEEEC
HPQQTGPLIVFTQAVLERGSFWTRTLQPQHADGRQLDVECAGTLMPTAEGRLLLLTLCDL
CCCCCCCEEEEEHHHHHCCCCEEECCCCCCCCCCEEEEEECCEECCCCCCCEEEEEECCC
DARHRRDLEARAEAFMHEGLAEWQRADRLFADIERENRLILRAVGEGIYGVNAEGRTTFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCCCCEEEE
NPAAEQILGWTAEEMIGRDMHAMVHHHHHDGSAYPNTTCPIYAAFRDGAVHHVENEVFWH
CHHHHHHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHCCCCEEEE
RSGRPVWVEYTSTPLRDRGRLIGAVVVFRDVTQKREANEQLRAALAEVDRLRERLEMENA
CCCCEEEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQEEIRAETSHRGIIGRSEAIKKVLRQVDLVAPTDATVLITGESGTGKELIARAIHDAS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
ERRERPLIRVNCAAIPRELFESEFFGHVRGAFTGALRDRVGRFELADRGTLFLDEVGEIP
HHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEHHHCCCC
LELQSKLLRVLQEGQFERIGEERTRQVDVRIIAATNRDLKAAVRAGRFREDLFYRLDVMP
HHHHHHHHHHHHCCCHHHHHHHHCEEEEEEEEEECCCHHHHHHHHCCHHHHHHHEEECCC
IESVPLRERREDIPLLAAHFLDGVRRKPADGPLQLSAGDMRRLQTYDWPGNVRELQNVIE
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCEEECCCCHHHHCCCCCCCCHHHHHHHHH
RGIILARNGRLHIDLPDREAPATAQPVPPSAELETEADRRLRLRTSILAALEAAGGKVSG
CCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
PGGAAERLGLKPTTLASRMKTLGITPGRASRSGA
CCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]