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The map label for this gene is poxB [H]

Identifier: 158422346

GI number: 158422346

Start: 807589

End: 809328

Strand: Reverse

Name: poxB [H]

Synonym: AZC_0722

Alternate gene names: 158422346

Gene position: 809328-807589 (Counterclockwise)

Preceding gene: 158422348

Following gene: 158422340

Centisome position: 15.07

GC content: 67.3

Gene sequence:

>1740_bases
ATGGAGGACCTCATGGCCGGAGTACGAGTTGCGGACGTCATGGCGCGCACGCTGGCGGCCGCCGGTGTGGAGCGTGTGTG
GGGCGTGGTGGGCGACAGCCTCAACGGCTTCACCGACGCCCTGCGCACCATTCCGCAGGTGCAGTGGATGCACGTGCGCC
ACGAGGAAGTGGCCGCTTTCGCCGCCGGCGCCGAAGCGCAGCTCACCGGCAAGCTCGCCGTGTGTGCCGGCTCCTGCGGG
CCGGGCAACCTCCACCTCATCAATGGCCTGTTCGATTGCCACCGCACCCGCACGCCGGTGCTGGCCATCGCTGCCCATAT
TCCGAGCGCGGAGATCGGCTCGGGCTATTTCCAGGAGACGCATCCCGAACAACTGTTCAAGGAGTGCAGCCACTACTGCG
AATTCGTCTCCAGCCCCTCCCAGATGCCCCGCATCATGGAGATCGCCATGCGCACGGCGATCCAGAAGCAGGGCGTGGCG
GTGATCGTCATTCCCGGCGACGTGGCGCTCCAGGCGGCGGAAGGCCCGACCGAGCCACGCTGGACCGCCATCGCCCCGCC
CACCGTCGTGCCGGCTGACAGCGAGCTTGAAAAGCTCATGGCGCTGGTCGACGGCGCGAGGCGCATCACCATCCTCGCCG
GCGGCGGCTGCGAGGGTGCCCATGACGAGGTGGTGGCCCTCGCCGCCGCGCTGAAGGCGCCCATGGTTCACACGCTGCGC
GGCAAGCCCTTCGTGGAATATGACAATCCCTACGACGTGGGCATGACCGGCTTCATCGGTTTCCCCTCCGGCTATCACGC
CATGCTGGAGGCCGACCTGCTGCTGATGGTGGGCACCGACTTCCCCTATCGCCAGTTCTATCCCACCGACGCCAAGGTGG
CGCAGATCGACATCAGGCCGGAAAACCTCGGCCGCCGCACGCGGCTCGACCTCGGCCTCGTGGGCGATGTGAAGGCGACC
CTCGCCACCCTGCTGCCGCGCCTGAAGCCCCGCACGGACGGGGATTTCCTCGCCAAGGTGCAGGAGCATTACAAGACCTC
CCGCCGCGACCTCGACGCGCTGGCGGTGGGCAATCCGGGCACCAAGCCCATCCATCCGCAATATCTCGCCAAGGTGATCG
ACGAAGTCGCGGCGCAGGACGCGCTGTTCAGCGTGGATGTGGGCGAGACGACGGTCTGGGCCGCGCGCTACATCGCCATG
AACGGCAAGCGCCGGCTCATCGGCTCGCTGATCCACGGCTCCATGGCCAATGCCATGCCGCAGGCGCTGGGCGCGCAGGC
GGCCCATCCCGGCCGGCAGGTGGTCTCGCTTTCGGGCGATGGCGGCTTCGCCATGCTGATGGGCGACATCCTCTCGCTCC
GCCAGCTCCAGCTGCCGGTGAAGATCGTGATCTTCAACAATGGCCTGCTGGGCTTCGTGGACATGGAGATGAAGGCCGCG
GGCTTCCTGAACGTGGGCACGGACCTTCAGAACCCGGATTTCGCCGCCATGGCCAATGCCATCGGCATCCTCGGCATCCG
CGTGGAAGACCCCGCCGACGTGAAGGGCGCACTTCAGAAGGCCTTTGCCCATGACGGCCCGGCGGTGGTGGACGTGGTGA
CGGACAAGATGGAACTGGTGATGCCCCCGGCCATCAAGGCCGAGCAGGTGGCCGGCTTCTCGCTCTATGCCGCCCGCGCC
ATCCTGAACGGGCGCGGCGACGAACTGCTCGAACTCGCCAAGCGCAACCTCTTCCGCTGA

Upstream 100 bases:

>100_bases
GATAGCCATGCATCTTGCCGACATGCTTTCCGGATATGCCGGGAACCGCAAGGCACGCAATGACTTGCCCCAGGCAATGA
GGGCGGGCCGCAGCAAACGG

Downstream 100 bases:

>100_bases
GGCGCGAGAAGGCGGCGGGCCACCCGCCGCCTTCGGATCATCGAAGAGCCGGGCGCTCCCCCGCCTCAGATCGGCCCGCA
ATCGGGCGGCACGCGCAGGT

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MEDLMAGVRVADVMARTLAAAGVERVWGVVGDSLNGFTDALRTIPQVQWMHVRHEEVAAFAAGAEAQLTGKLAVCAGSCG
PGNLHLINGLFDCHRTRTPVLAIAAHIPSAEIGSGYFQETHPEQLFKECSHYCEFVSSPSQMPRIMEIAMRTAIQKQGVA
VIVIPGDVALQAAEGPTEPRWTAIAPPTVVPADSELEKLMALVDGARRITILAGGGCEGAHDEVVALAAALKAPMVHTLR
GKPFVEYDNPYDVGMTGFIGFPSGYHAMLEADLLLMVGTDFPYRQFYPTDAKVAQIDIRPENLGRRTRLDLGLVGDVKAT
LATLLPRLKPRTDGDFLAKVQEHYKTSRRDLDALAVGNPGTKPIHPQYLAKVIDEVAAQDALFSVDVGETTVWAARYIAM
NGKRRLIGSLIHGSMANAMPQALGAQAAHPGRQVVSLSGDGGFAMLMGDILSLRQLQLPVKIVIFNNGLLGFVDMEMKAA
GFLNVGTDLQNPDFAAMANAIGILGIRVEDPADVKGALQKAFAHDGPAVVDVVTDKMELVMPPAIKAEQVAGFSLYAARA
ILNGRGDELLELAKRNLFR

Sequences:

>Translated_579_residues
MEDLMAGVRVADVMARTLAAAGVERVWGVVGDSLNGFTDALRTIPQVQWMHVRHEEVAAFAAGAEAQLTGKLAVCAGSCG
PGNLHLINGLFDCHRTRTPVLAIAAHIPSAEIGSGYFQETHPEQLFKECSHYCEFVSSPSQMPRIMEIAMRTAIQKQGVA
VIVIPGDVALQAAEGPTEPRWTAIAPPTVVPADSELEKLMALVDGARRITILAGGGCEGAHDEVVALAAALKAPMVHTLR
GKPFVEYDNPYDVGMTGFIGFPSGYHAMLEADLLLMVGTDFPYRQFYPTDAKVAQIDIRPENLGRRTRLDLGLVGDVKAT
LATLLPRLKPRTDGDFLAKVQEHYKTSRRDLDALAVGNPGTKPIHPQYLAKVIDEVAAQDALFSVDVGETTVWAARYIAM
NGKRRLIGSLIHGSMANAMPQALGAQAAHPGRQVVSLSGDGGFAMLMGDILSLRQLQLPVKIVIFNNGLLGFVDMEMKAA
GFLNVGTDLQNPDFAAMANAIGILGIRVEDPADVKGALQKAFAHDGPAVVDVVTDKMELVMPPAIKAEQVAGFSLYAARA
ILNGRGDELLELAKRNLFR
>Mature_579_residues
MEDLMAGVRVADVMARTLAAAGVERVWGVVGDSLNGFTDALRTIPQVQWMHVRHEEVAAFAAGAEAQLTGKLAVCAGSCG
PGNLHLINGLFDCHRTRTPVLAIAAHIPSAEIGSGYFQETHPEQLFKECSHYCEFVSSPSQMPRIMEIAMRTAIQKQGVA
VIVIPGDVALQAAEGPTEPRWTAIAPPTVVPADSELEKLMALVDGARRITILAGGGCEGAHDEVVALAAALKAPMVHTLR
GKPFVEYDNPYDVGMTGFIGFPSGYHAMLEADLLLMVGTDFPYRQFYPTDAKVAQIDIRPENLGRRTRLDLGLVGDVKAT
LATLLPRLKPRTDGDFLAKVQEHYKTSRRDLDALAVGNPGTKPIHPQYLAKVIDEVAAQDALFSVDVGETTVWAARYIAM
NGKRRLIGSLIHGSMANAMPQALGAQAAHPGRQVVSLSGDGGFAMLMGDILSLRQLQLPVKIVIFNNGLLGFVDMEMKAA
GFLNVGTDLQNPDFAAMANAIGILGIRVEDPADVKGALQKAFAHDGPAVVDVVTDKMELVMPPAIKAEQVAGFSLYAARA
ILNGRGDELLELAKRNLFR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=561, Percent_Identity=24.0641711229947, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1787096, Length=570, Percent_Identity=61.7543859649123, Blast_Score=721, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=28.7795992714025, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=26.5342960288809, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1786717, Length=571, Percent_Identity=27.3204903677758, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788716, Length=549, Percent_Identity=22.2222222222222, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17531299, Length=555, Percent_Identity=27.2072072072072, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=26.5709156193896, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=574, Percent_Identity=23.8675958188153, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=480, Percent_Identity=23.3333333333333, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=24.468085106383, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62032; Mature: 62032

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDLMAGVRVADVMARTLAAAGVERVWGVVGDSLNGFTDALRTIPQVQWMHVRHEEVAAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHEEEHHHHHHHHH
AAGAEAQLTGKLAVCAGSCGPGNLHLINGLFDCHRTRTPVLAIAAHIPSAEIGSGYFQET
HCCCCCEEECEEEEEECCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCC
HPEQLFKECSHYCEFVSSPSQMPRIMEIAMRTAIQKQGVAVIVIPGDVALQAAEGPTEPR
CHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCC
WTAIAPPTVVPADSELEKLMALVDGARRITILAGGGCEGAHDEVVALAAALKAPMVHTLR
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHC
GKPFVEYDNPYDVGMTGFIGFPSGYHAMLEADLLLMVGTDFPYRQFYPTDAKVAQIDIRP
CCEEEECCCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEECH
ENLGRRTRLDLGLVGDVKATLATLLPRLKPRTDGDFLAKVQEHYKTSRRDLDALAVGNPG
HHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCC
TKPIHPQYLAKVIDEVAAQDALFSVDVGETTVWAARYIAMNGKRRLIGSLIHGSMANAMP
CCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QALGAQAAHPGRQVVSLSGDGGFAMLMGDILSLRQLQLPVKIVIFNNGLLGFVDMEMKAA
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEHHHC
GFLNVGTDLQNPDFAAMANAIGILGIRVEDPADVKGALQKAFAHDGPAVVDVVTDKMELV
CEEECCCCCCCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHC
MPPAIKAEQVAGFSLYAARAILNGRGDELLELAKRNLFR
CCCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MEDLMAGVRVADVMARTLAAAGVERVWGVVGDSLNGFTDALRTIPQVQWMHVRHEEVAAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHEEEHHHHHHHHH
AAGAEAQLTGKLAVCAGSCGPGNLHLINGLFDCHRTRTPVLAIAAHIPSAEIGSGYFQET
HCCCCCEEECEEEEEECCCCCCCEEEEHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCC
HPEQLFKECSHYCEFVSSPSQMPRIMEIAMRTAIQKQGVAVIVIPGDVALQAAEGPTEPR
CHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCC
WTAIAPPTVVPADSELEKLMALVDGARRITILAGGGCEGAHDEVVALAAALKAPMVHTLR
EEEECCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHC
GKPFVEYDNPYDVGMTGFIGFPSGYHAMLEADLLLMVGTDFPYRQFYPTDAKVAQIDIRP
CCEEEECCCCCCCCCEEEEECCCCHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEEEECH
ENLGRRTRLDLGLVGDVKATLATLLPRLKPRTDGDFLAKVQEHYKTSRRDLDALAVGNPG
HHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCC
TKPIHPQYLAKVIDEVAAQDALFSVDVGETTVWAARYIAMNGKRRLIGSLIHGSMANAMP
CCCCCHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QALGAQAAHPGRQVVSLSGDGGFAMLMGDILSLRQLQLPVKIVIFNNGLLGFVDMEMKAA
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEHHHC
GFLNVGTDLQNPDFAAMANAIGILGIRVEDPADVKGALQKAFAHDGPAVVDVVTDKMELV
CEEECCCCCCCCCHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHC
MPPAIKAEQVAGFSLYAARAILNGRGDELLELAKRNLFR
CCCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]