Click here to switch to the map view.

The map label for this gene is rutF2 [H]

Identifier: 158422340

GI number: 158422340

Start: 800925

End: 801530

Strand: Reverse

Name: rutF2 [H]

Synonym: AZC_0716

Alternate gene names: 158422340

Gene position: 801530-800925 (Counterclockwise)

Preceding gene: 158422346

Following gene: 158422325

Centisome position: 14.93

GC content: 71.29

Gene sequence:

>606_bases
TTGCACAGCAACATCCGTCCCCGCCGGGACTTCAGGTCCGGAACGATCCATGCCCGCCTTGCCGCCCTCCGCCTCGCCCG
CCGGGACCCGCGCGTGACCGCGCCCGCCGCCACCGCCGGCCACTCCCCCTCCGAGGGCTATCGCGCGGGCATGCGCCATC
TTGCGGGCGCCGTGAGCGTGCTCACCGTGGGCGAGGGCGACAGCCGAACCGGCCTCACCGCCTCTTCGCTCACCTCCTTC
GCGGTGGACCCGCCGACCCTTCTCATCTGCATCAACAAGGCCTCGTCCGTGCGCGCGGAACTCCTGGCGCGCAAGGTTTT
CGCCGTGAATGTCCTGGCGGAGGGCCAGCGCCGCGTGGCGGAGACCTTCGCCGGCATGACCGGCCTGCACGGGGCGGCAC
GATTCGAGGGCACGCCCTGGTCGGTGATGGAGACGGGGGCGCCGGTGATCGACGACGCGCTCGTCGCCTTCGACTGCCGG
CTCGACGAGATCATCGAGCGCCATTCTCACATGATTGTGCTCGGCCGCGTGCTGGCGACGCGCGTCGACGATACGGCGCG
TCCCCTCCTCTACTGGCACCGGGACTTCCGCACCCTCGCCCCCTGA

Upstream 100 bases:

>100_bases
CGCACCCGCGCTGCCAATCGCCAAGAACTGACCTAATTTGGTTTGATCCAGCCTGAGCTGTGCACTCGTACCCTCGCAAA
GCGGGCGCGCCCCGCCTACA

Downstream 100 bases:

>100_bases
GTCGCTGCGGCGGCCCAAGGCAAATGGACAAACAAAAAGGCGGGCCCCGAAGGACCCGCCTTTTGCATTCCCGAACGACC
GGCCCGCAGCTCAGGCGGCG

Product: actinorhodin polyketide dimerase

Products: NA

Alternate protein names: FMN reductase 2; NAD(P)H:flavin oxidoreductase 2 [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MHSNIRPRRDFRSGTIHARLAALRLARRDPRVTAPAATAGHSPSEGYRAGMRHLAGAVSVLTVGEGDSRTGLTASSLTSF
AVDPPTLLICINKASSVRAELLARKVFAVNVLAEGQRRVAETFAGMTGLHGAARFEGTPWSVMETGAPVIDDALVAFDCR
LDEIIERHSHMIVLGRVLATRVDDTARPLLYWHRDFRTLAP

Sequences:

>Translated_201_residues
MHSNIRPRRDFRSGTIHARLAALRLARRDPRVTAPAATAGHSPSEGYRAGMRHLAGAVSVLTVGEGDSRTGLTASSLTSF
AVDPPTLLICINKASSVRAELLARKVFAVNVLAEGQRRVAETFAGMTGLHGAARFEGTPWSVMETGAPVIDDALVAFDCR
LDEIIERHSHMIVLGRVLATRVDDTARPLLYWHRDFRTLAP
>Mature_201_residues
MHSNIRPRRDFRSGTIHARLAALRLARRDPRVTAPAATAGHSPSEGYRAGMRHLAGAVSVLTVGEGDSRTGLTASSLTSF
AVDPPTLLICINKASSVRAELLARKVFAVNVLAEGQRRVAETFAGMTGLHGAARFEGTPWSVMETGAPVIDDALVAFDCR
LDEIIERHSHMIVLGRVLATRVDDTARPLLYWHRDFRTLAP

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 21830; Mature: 21830

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSNIRPRRDFRSGTIHARLAALRLARRDPRVTAPAATAGHSPSEGYRAGMRHLAGAVSV
CCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEE
LTVGEGDSRTGLTASSLTSFAVDPPTLLICINKASSVRAELLARKVFAVNVLAEGQRRVA
EEECCCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFAGMTGLHGAARFEGTPWSVMETGAPVIDDALVAFDCRLDEIIERHSHMIVLGRVLAT
HHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCEEHHHHHHHH
RVDDTARPLLYWHRDFRTLAP
HCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHSNIRPRRDFRSGTIHARLAALRLARRDPRVTAPAATAGHSPSEGYRAGMRHLAGAVSV
CCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEE
LTVGEGDSRTGLTASSLTSFAVDPPTLLICINKASSVRAELLARKVFAVNVLAEGQRRVA
EEECCCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ETFAGMTGLHGAARFEGTPWSVMETGAPVIDDALVAFDCRLDEIIERHSHMIVLGRVLAT
HHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCEEHHHHHHHH
RVDDTARPLLYWHRDFRTLAP
HCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA