| Definition | Azorhizobium caulinodans ORS 571, complete genome. |
|---|---|
| Accession | NC_009937 |
| Length | 5,369,772 |
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The map label for this gene is nasA [H]
Identifier: 158422303
GI number: 158422303
Start: 747820
End: 750519
Strand: Reverse
Name: nasA [H]
Synonym: AZC_0679
Alternate gene names: 158422303
Gene position: 750519-747820 (Counterclockwise)
Preceding gene: 158422304
Following gene: 158422302
Centisome position: 13.98
GC content: 72.04
Gene sequence:
>2700_bases ATGAACGCCCCGCTTTCCTCGCCTCAGGCGACATCGACCACCTGTCCCTATTGCGGCGTCGGCTGCGGCGTGAAGGCGAC GCCCGATGGGCTCGGCGGCGCATTGATCGAGGGCGATGCCGCGCACCCGGCCAATCTCGGCCGGCTGTGCTCCAAGGGCG CGGCGCTGGGCGAGACCATCGGGCTGGAGACCCGGCTGCTCCATCCCATGATGCGCGAGGGCGCCGGCGATCTGCGGCGG GTGACGTGGGATTCCGCACTCGACCGGGTGGCGGAGGGCTTCCGCCGCGTGCTCGCCGAGCATGGGCCGCAGGCGGTGGC CTTCTATCTCTCCGGCCAGTTGCTCACGGAGGATTATTACGCCGCCAACAAGCTCGCCAAGGGCTTCCTCGGCACGGCGA ACGTGGACACCAATTCGCGCCTCTGCATGGCTTCGTCCGTCGCCGGGCACAAGCGGGCCTTCGGCTCGGATACGGTGCCG GGATCGTATGAGGATCTGGAACAAGCCGACCTCATCGTCCTCACAGGCTCCAACACCGCCTGGTGCCATCCGGTGCTGTT CCGCCGCATGGTGGCGGCGCGGCAGGCGCGCGGCACGAAGCTCGTGGTCATCGACCCCCGCCGCACCGCCACGGCCGAGG AAGCGGACCTGTTTCTGCCCCTCGCCCACGGCACGGACGCGGCGCTGTTCTCCGGCCTGCTGGTGCATCTGACGGACACC GGCGCGCTCGATGAGACCTTCATCGCGCAGCACACCGAGGGATTTGCGGAAACGCTGTCGGCCGCGCGGGCGGAAACGCC GGACCTTGCCGCCACCGCCCGCATCACGGGCTTAGCCGAAGCGGATGTGGCGCGCTTCTTCGCGCTGTTCCGGGCCACGG ACCGAGTGGTGACCTGCTACTCGCAGGGCGTCAACCAGTCCCGGCGCGGCACGGACAAGGTAAACGCCATCCTCAACTGC CACCTCGCCACCGGCCGCATCGGGCGCGAAGGCATGGGGCCCTTCTCGCTCACCGGCCAGCCCAATGCCATGGGCGGGCG CGAGGTGGGCGGGCTCGCCAACCAGTTGGCCGCCCATATGGGCTTCTCTCCCGCTGAAGTGGACCGCGTGCGCCGCTTCT GGGGCGCGCCCCACATGGCGCAGGCGGAGGGGCTGAAGGCGGTGGACATGTTCGCCGCCATGGAGCGCGGAGAGATCAAG GCGCTGTGGGTCATGGGCACCAATCCGGCCGTCAGCCTGCCGCGCGCGGATGCCGCCCGACGTGCCATGGCCGGACTCGA CCTCTTCGTGCTGTCCGAGAACGTGCTCGGTACGGACACCGCCGGCTGCGCCCCCCATGTGCTGCTGCCCGCCGTCGCCT GGGGCGAGAAAGACGGCACGGTGACGAATTCCGAACGCCGCATCTCCCGCCAACGCCCTTTCCTCGCCCCCGCCGGCGAG GCGAAGCCCGACTGGTGGGTGCTGGCGCAGGTGGCCCGCCGCCTCGGCCATGGGGAGGCTTTCGACTGGCGCGGCCCGGC GGACATCTTCCGCGAGCACGCGGCCCTCTCCGCTTTCGAGAACAACGGCACGCGCGACTTCGATCTCTCCGGCCTCGCGG ACCTGACCGATGCCGCCTATGAGACCCTCGCCCCGGTGCAATGGCCGGTGCGCACGGCGTCGGACGGGCCGCATGGCACC GAACGGCTGTTCGGCACCGGCGGCTTCTTCACCGCCTCCCGCCGAGCGCGCTTCGTGACCCCCGCCCCCGTCGCCCCGGC CCCGGTGGCGGAAGACTTCCCGCTGCTGCTCAACACCGGCCGCATCCGCGACCAGTGGCACACCATGACCCGCACGGGCC TCTCGCCCCGGCTCGGCAGCCACATCTCCGCGCCCTTCGTGACCGTGCATCCGGACGACGCGCGGGCCGCCGGCCTGACC GACGGCGCTCTGGCGCAGGTGGAAACCGCCCATGGCCGCGCCGTGCTGGAGGTGCGCCTCGACGACGGCCAGCGGCCCGG CACGGTGTTCGCACCCATCCATTGGAGCGCGACCAATTCCTCCGGCGGGCGCGTGGGCGCGCTGGTGCATCCGCTCACCG ATCCCGTCTCCGGCCAGCCGGACCTCAAGGCGACGCCGGCCCGCCTCGCGCCGGTGGACGCGCCCGCGCGGGGGGTCCTG CTCGCCCGCAGCCGCGTCTCGCCACCGCCGGGCCTTGGTTTTGCCCGCGCGCGGCTGGAGGGTGGACACGGCTGGCGGCT CGCGGGCTCTGTCGATGTCGCCGCATGGGCCGCCTATGCGCAGGCCCTCGCCGACGATCTTGGCCTTGCGGACAGCTTCT GCGACATGGCGGACACGGCGGGCGGCCATTATCGCGCCGCCGCCTTCACCGCCTCGGGCGCGCTCGCCTTTGCTCTGGTG CTGGCGCCGGAGGCCGAGGTGCCGGCCTGGGATGTGTGGGTGCAGCGGCTCGCCCGCGGCACGCTCACCGAGGACGATCG GCGCCTCGTGCTCTCCGGCCGCGCTTCGGATGGGGCAGCCGCCTGCGGGCCGCTGGTCTGCGCCTGCTTCGGCGTGCCGC GCGGCGCGATCGCGCAGGCCATCCGCGAGGGGGCGCATGATGCCGCCGCCATCGGCGCGCGGCTCAAGGCTGGCACCAAT TGCGGCTCCTGCCTTCCGGAGCTGAAAGCGCTGCTCGTGGACATGCTGGCCCCCGCCTGA
Upstream 100 bases:
>100_bases CGGGGCCGAGGGCGGTGCCCGCTTCGTGGCGGAACTGAAGAAGGCCGGCCGCTATCAGGCGGACGTCTACTGAGCGCAGG AAAACCGGAGACAGCGCCTC
Downstream 100 bases:
>100_bases CGCCCGCTCGACAGCGGGCACAAGCGATGGCAAACGCACGCCATGTTTCAGCGTGCATTGCTTGCGGTTGTCACCGCCTC GATTGTGATGATGTCAGGGC
Product: nitrate reductase large subunit protein
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 899; Mature: 899
Protein sequence:
>899_residues MNAPLSSPQATSTTCPYCGVGCGVKATPDGLGGALIEGDAAHPANLGRLCSKGAALGETIGLETRLLHPMMREGAGDLRR VTWDSALDRVAEGFRRVLAEHGPQAVAFYLSGQLLTEDYYAANKLAKGFLGTANVDTNSRLCMASSVAGHKRAFGSDTVP GSYEDLEQADLIVLTGSNTAWCHPVLFRRMVAARQARGTKLVVIDPRRTATAEEADLFLPLAHGTDAALFSGLLVHLTDT GALDETFIAQHTEGFAETLSAARAETPDLAATARITGLAEADVARFFALFRATDRVVTCYSQGVNQSRRGTDKVNAILNC HLATGRIGREGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFSPAEVDRVRRFWGAPHMAQAEGLKAVDMFAAMERGEIK ALWVMGTNPAVSLPRADAARRAMAGLDLFVLSENVLGTDTAGCAPHVLLPAVAWGEKDGTVTNSERRISRQRPFLAPAGE AKPDWWVLAQVARRLGHGEAFDWRGPADIFREHAALSAFENNGTRDFDLSGLADLTDAAYETLAPVQWPVRTASDGPHGT ERLFGTGGFFTASRRARFVTPAPVAPAPVAEDFPLLLNTGRIRDQWHTMTRTGLSPRLGSHISAPFVTVHPDDARAAGLT DGALAQVETAHGRAVLEVRLDDGQRPGTVFAPIHWSATNSSGGRVGALVHPLTDPVSGQPDLKATPARLAPVDAPARGVL LARSRVSPPPGLGFARARLEGGHGWRLAGSVDVAAWAAYAQALADDLGLADSFCDMADTAGGHYRAAAFTASGALAFALV LAPEAEVPAWDVWVQRLARGTLTEDDRRLVLSGRASDGAAACGPLVCACFGVPRGAIAQAIREGAHDAAAIGARLKAGTN CGSCLPELKALLVDMLAPA
Sequences:
>Translated_899_residues MNAPLSSPQATSTTCPYCGVGCGVKATPDGLGGALIEGDAAHPANLGRLCSKGAALGETIGLETRLLHPMMREGAGDLRR VTWDSALDRVAEGFRRVLAEHGPQAVAFYLSGQLLTEDYYAANKLAKGFLGTANVDTNSRLCMASSVAGHKRAFGSDTVP GSYEDLEQADLIVLTGSNTAWCHPVLFRRMVAARQARGTKLVVIDPRRTATAEEADLFLPLAHGTDAALFSGLLVHLTDT GALDETFIAQHTEGFAETLSAARAETPDLAATARITGLAEADVARFFALFRATDRVVTCYSQGVNQSRRGTDKVNAILNC HLATGRIGREGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFSPAEVDRVRRFWGAPHMAQAEGLKAVDMFAAMERGEIK ALWVMGTNPAVSLPRADAARRAMAGLDLFVLSENVLGTDTAGCAPHVLLPAVAWGEKDGTVTNSERRISRQRPFLAPAGE AKPDWWVLAQVARRLGHGEAFDWRGPADIFREHAALSAFENNGTRDFDLSGLADLTDAAYETLAPVQWPVRTASDGPHGT ERLFGTGGFFTASRRARFVTPAPVAPAPVAEDFPLLLNTGRIRDQWHTMTRTGLSPRLGSHISAPFVTVHPDDARAAGLT DGALAQVETAHGRAVLEVRLDDGQRPGTVFAPIHWSATNSSGGRVGALVHPLTDPVSGQPDLKATPARLAPVDAPARGVL LARSRVSPPPGLGFARARLEGGHGWRLAGSVDVAAWAAYAQALADDLGLADSFCDMADTAGGHYRAAAFTASGALAFALV LAPEAEVPAWDVWVQRLARGTLTEDDRRLVLSGRASDGAAACGPLVCACFGVPRGAIAQAIREGAHDAAAIGARLKAGTN CGSCLPELKALLVDMLAPA >Mature_899_residues MNAPLSSPQATSTTCPYCGVGCGVKATPDGLGGALIEGDAAHPANLGRLCSKGAALGETIGLETRLLHPMMREGAGDLRR VTWDSALDRVAEGFRRVLAEHGPQAVAFYLSGQLLTEDYYAANKLAKGFLGTANVDTNSRLCMASSVAGHKRAFGSDTVP GSYEDLEQADLIVLTGSNTAWCHPVLFRRMVAARQARGTKLVVIDPRRTATAEEADLFLPLAHGTDAALFSGLLVHLTDT GALDETFIAQHTEGFAETLSAARAETPDLAATARITGLAEADVARFFALFRATDRVVTCYSQGVNQSRRGTDKVNAILNC HLATGRIGREGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFSPAEVDRVRRFWGAPHMAQAEGLKAVDMFAAMERGEIK ALWVMGTNPAVSLPRADAARRAMAGLDLFVLSENVLGTDTAGCAPHVLLPAVAWGEKDGTVTNSERRISRQRPFLAPAGE AKPDWWVLAQVARRLGHGEAFDWRGPADIFREHAALSAFENNGTRDFDLSGLADLTDAAYETLAPVQWPVRTASDGPHGT ERLFGTGGFFTASRRARFVTPAPVAPAPVAEDFPLLLNTGRIRDQWHTMTRTGLSPRLGSHISAPFVTVHPDDARAAGLT DGALAQVETAHGRAVLEVRLDDGQRPGTVFAPIHWSATNSSGGRVGALVHPLTDPVSGQPDLKATPARLAPVDAPARGVL LARSRVSPPPGLGFARARLEGGHGWRLAGSVDVAAWAAYAQALADDLGLADSFCDMADTAGGHYRAAAFTASGALAFALV LAPEAEVPAWDVWVQRLARGTLTEDDRRLVLSGRASDGAAACGPLVCACFGVPRGAIAQAIREGAHDAAAIGARLKAGTN CGSCLPELKALLVDMLAPA
Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]
COG id: COG0243
COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]
Homologues:
Organism=Escherichia coli, GI3868721, Length=713, Percent_Identity=27.0687237026648, Blast_Score=284, Evalue=2e-77, Organism=Escherichia coli, GI1788534, Length=800, Percent_Identity=28.5, Blast_Score=273, Evalue=3e-74, Organism=Escherichia coli, GI3868719, Length=628, Percent_Identity=24.6815286624204, Blast_Score=154, Evalue=3e-38, Organism=Escherichia coli, GI3868720, Length=594, Percent_Identity=25.2525252525253, Blast_Score=152, Evalue=1e-37, Organism=Escherichia coli, GI1787778, Length=709, Percent_Identity=25.9520451339915, Blast_Score=127, Evalue=2e-30, Organism=Escherichia coli, GI1787870, Length=755, Percent_Identity=22.7814569536424, Blast_Score=105, Evalue=1e-23, Organism=Escherichia coli, GI171474008, Length=544, Percent_Identity=22.4264705882353, Blast_Score=93, Evalue=6e-20, Organism=Escherichia coli, GI87081797, Length=316, Percent_Identity=25, Blast_Score=79, Evalue=1e-15, Organism=Escherichia coli, GI145693196, Length=495, Percent_Identity=24.8484848484848, Blast_Score=79, Evalue=2e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR009010 - InterPro: IPR007419 - InterPro: IPR006657 - InterPro: IPR006656 - InterPro: IPR006963 - InterPro: IPR006655 [H]
Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]
EC number: =1.7.99.4 [H]
Molecular weight: Translated: 94564; Mature: 94564
Theoretical pI: Translated: 6.72; Mature: 6.72
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNAPLSSPQATSTTCPYCGVGCGVKATPDGLGGALIEGDAAHPANLGRLCSKGAALGETI CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHH GLETRLLHPMMREGAGDLRRVTWDSALDRVAEGFRRVLAEHGPQAVAFYLSGQLLTEDYY CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEHHHHH AANKLAKGFLGTANVDTNSRLCMASSVAGHKRAFGSDTVPGSYEDLEQADLIVLTGSNTA HHHHHHHCCCCCCCCCCCCCEEEEHHCCHHHHCCCCCCCCCCHHHHCCCCEEEEECCCCC WCHPVLFRRMVAARQARGTKLVVIDPRRTATAEEADLFLPLAHGTDAALFSGLLVHLTDT CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHCCEEEEEECC GALDETFIAQHTEGFAETLSAARAETPDLAATARITGLAEADVARFFALFRATDRVVTCY CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH SQGVNQSRRGTDKVNAILNCHLATGRIGREGMGPFSLTGQPNAMGGREVGGLANQLAAHM HHCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHC GFSPAEVDRVRRFWGAPHMAQAEGLKAVDMFAAMERGEIKALWVMGTNPAVSLPRADAAR CCCHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHH RAMAGLDLFVLSENVLGTDTAGCAPHVLLPAVAWGEKDGTVTNSERRISRQRPFLAPAGE HHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC AKPDWWVLAQVARRLGHGEAFDWRGPADIFREHAALSAFENNGTRDFDLSGLADLTDAAY CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH ETLAPVQWPVRTASDGPHGTERLFGTGGFFTASRRARFVTPAPVAPAPVAEDFPLLLNTG HHHCCCCCCCCCCCCCCCCCHHHEECCCEEECCCCCEEECCCCCCCCCCCCCCCEEEECC RIRDQWHTMTRTGLSPRLGSHISAPFVTVHPDDARAAGLTDGALAQVETAHGRAVLEVRL CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHEEECCCCEEEEEEE DDGQRPGTVFAPIHWSATNSSGGRVGALVHPLTDPVSGQPDLKATPARLAPVDAPARGVL CCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE LARSRVSPPPGLGFARARLEGGHGWRLAGSVDVAAWAAYAQALADDLGLADSFCDMADTA EECCCCCCCCCCCHHHHEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC GGHYRAAAFTASGALAFALVLAPEAEVPAWDVWVQRLARGTLTEDDRRLVLSGRASDGAA CCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH ACGPLVCACFGVPRGAIAQAIREGAHDAAAIGARLKAGTNCGSCLPELKALLVDMLAPA HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MNAPLSSPQATSTTCPYCGVGCGVKATPDGLGGALIEGDAAHPANLGRLCSKGAALGETI CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHH GLETRLLHPMMREGAGDLRRVTWDSALDRVAEGFRRVLAEHGPQAVAFYLSGQLLTEDYY CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEHHHHH AANKLAKGFLGTANVDTNSRLCMASSVAGHKRAFGSDTVPGSYEDLEQADLIVLTGSNTA HHHHHHHCCCCCCCCCCCCCEEEEHHCCHHHHCCCCCCCCCCHHHHCCCCEEEEECCCCC WCHPVLFRRMVAARQARGTKLVVIDPRRTATAEEADLFLPLAHGTDAALFSGLLVHLTDT CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHCCEEEEEECC GALDETFIAQHTEGFAETLSAARAETPDLAATARITGLAEADVARFFALFRATDRVVTCY CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH SQGVNQSRRGTDKVNAILNCHLATGRIGREGMGPFSLTGQPNAMGGREVGGLANQLAAHM HHCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHC GFSPAEVDRVRRFWGAPHMAQAEGLKAVDMFAAMERGEIKALWVMGTNPAVSLPRADAAR CCCHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHH RAMAGLDLFVLSENVLGTDTAGCAPHVLLPAVAWGEKDGTVTNSERRISRQRPFLAPAGE HHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCC AKPDWWVLAQVARRLGHGEAFDWRGPADIFREHAALSAFENNGTRDFDLSGLADLTDAAY CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH ETLAPVQWPVRTASDGPHGTERLFGTGGFFTASRRARFVTPAPVAPAPVAEDFPLLLNTG HHHCCCCCCCCCCCCCCCCCHHHEECCCEEECCCCCEEECCCCCCCCCCCCCCCEEEECC RIRDQWHTMTRTGLSPRLGSHISAPFVTVHPDDARAAGLTDGALAQVETAHGRAVLEVRL CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHEEECCCCEEEEEEE DDGQRPGTVFAPIHWSATNSSGGRVGALVHPLTDPVSGQPDLKATPARLAPVDAPARGVL CCCCCCCEEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE LARSRVSPPPGLGFARARLEGGHGWRLAGSVDVAAWAAYAQALADDLGLADSFCDMADTA EECCCCCCCCCCCHHHHEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCC GGHYRAAAFTASGALAFALVLAPEAEVPAWDVWVQRLARGTLTEDDRRLVLSGRASDGAA CCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCH ACGPLVCACFGVPRGAIAQAIREGAHDAAAIGARLKAGTNCGSCLPELKALLVDMLAPA HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8468296 [H]