Click here to switch to the map view.

The map label for this gene is hupH [H]

Identifier: 158422228

GI number: 158422228

Start: 670783

End: 671622

Strand: Direct

Name: hupH [H]

Synonym: AZC_0604

Alternate gene names: 158422228

Gene position: 670783-671622 (Clockwise)

Preceding gene: 158422227

Following gene: 158422229

Centisome position: 12.49

GC content: 70.71

Gene sequence:

>840_bases
ATGGCCTCGCCCTTTGCCATCCAGCCGCCCCTCGGATTCGGCCCCGGCAGCCAGTCGGGAGACGAGGAGGGCCTCGCGTA
TCTGCCCATGCCCTCGGGTATGCGGGTCTATGAGCCCCATCTTCTCGATGCGGACGTGGCGGGCCCCTGCGCCGCCGCGC
TCGCGACGCTGGCGGCGGTGCGCGATGCGCTGGCCCGCTGGCAGCCGGGTGGCCACGAGAGCTTTTCGCTGGCCGGGCTC
GATGCCCGGAACCGCGCCCTGATCGACGAGGCGCTGGGCGAGGGCGAAGTGGCGGTGCGCGTCACCGGCGTCGTGCCGTT
GGAGATCCAGGAAGCGACCTTCGCCGGCGTGTGGCGCGTGCGCGGCGCGGAGAGCGTCGATCGCATCGAGGTGGGGGCTT
TTCCGCGTGGCGCATTGCTGCGCGCCTTCCCGGCGGAATGGCAGATGGACCTTGAGGATATCGCCCGGCCGCGCCCGGGC
GTGGTGAACGCGCCGCCCATCCTGGCCGAGATCGCCCACCGCACCAGCGCCCGCGCGCCCGGTACGCCGGCCCACGTCAT
CAATTTCTCCCTGCTGCCGCACACGCCGGAAGACCTCGTGCTGATCGATGAGGTGCTGGGGCAGGGGCACCTCACCATTC
TCTCGCGCGGCTATGGCAATTGCCGGATCGATGCCGCCGCGCGGCGGGATGTGTGGCGGGTGCGCTATTACAATTCCACC
GACATGCTCATCCTCGACACGCTGGAGATCACGTCTCTGCCGGAGGTGGCGCAGGCCGCGCCCGAGGACATTGCCGACAG
CGCCGCGCGCCTTGCGCAGGTGCTGGACGCTCTGGCGTGA

Upstream 100 bases:

>100_bases
CGGAACGCCCATCGGCTCCGTCGCCCGCATGCGCGACTGGGATGAATATCTCGCCCGCCTGAGCGCCGTCCTCGGCGCGG
CCACCCACTGAGGAGCCTCC

Downstream 100 bases:

>100_bases
GGAGACCCGCGTGACCGCCTCCCTGCGCTTTGAAGGCTCCTATCGCGGTCAGGCCGCGACGCTCGACCCGGCGACCCGGC
TCGAATGCAAGATCTGCTGG

Product: hydrogenase expression/formation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MASPFAIQPPLGFGPGSQSGDEEGLAYLPMPSGMRVYEPHLLDADVAGPCAAALATLAAVRDALARWQPGGHESFSLAGL
DARNRALIDEALGEGEVAVRVTGVVPLEIQEATFAGVWRVRGAESVDRIEVGAFPRGALLRAFPAEWQMDLEDIARPRPG
VVNAPPILAEIAHRTSARAPGTPAHVINFSLLPHTPEDLVLIDEVLGQGHLTILSRGYGNCRIDAAARRDVWRVRYYNST
DMLILDTLEITSLPEVAQAAPEDIADSAARLAQVLDALA

Sequences:

>Translated_279_residues
MASPFAIQPPLGFGPGSQSGDEEGLAYLPMPSGMRVYEPHLLDADVAGPCAAALATLAAVRDALARWQPGGHESFSLAGL
DARNRALIDEALGEGEVAVRVTGVVPLEIQEATFAGVWRVRGAESVDRIEVGAFPRGALLRAFPAEWQMDLEDIARPRPG
VVNAPPILAEIAHRTSARAPGTPAHVINFSLLPHTPEDLVLIDEVLGQGHLTILSRGYGNCRIDAAARRDVWRVRYYNST
DMLILDTLEITSLPEVAQAAPEDIADSAARLAQVLDALA
>Mature_278_residues
ASPFAIQPPLGFGPGSQSGDEEGLAYLPMPSGMRVYEPHLLDADVAGPCAAALATLAAVRDALARWQPGGHESFSLAGLD
ARNRALIDEALGEGEVAVRVTGVVPLEIQEATFAGVWRVRGAESVDRIEVGAFPRGALLRAFPAEWQMDLEDIARPRPGV
VNAPPILAEIAHRTSARAPGTPAHVINFSLLPHTPEDLVLIDEVLGQGHLTILSRGYGNCRIDAAARRDVWRVRYYNSTD
MLILDTLEITSLPEVAQAAPEDIADSAARLAQVLDALA

Specific function: Not Known. Could Enhance The Incorporation Of Nickel To The Hydrogenase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=279, Percent_Identity=34.7670250896057, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 29744; Mature: 29613

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPFAIQPPLGFGPGSQSGDEEGLAYLPMPSGMRVYEPHLLDADVAGPCAAALATLAAV
CCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCEECCCCCCHHHHHHHHHHHH
RDALARWQPGGHESFSLAGLDARNRALIDEALGEGEVAVRVTGVVPLEIQEATFAGVWRV
HHHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCCEEEEEEEEEEEEECCCHHEEEEEE
RGAESVDRIEVGAFPRGALLRAFPAEWQMDLEDIARPRPGVVNAPPILAEIAHRTSARAP
CCCCCCCEEEECCCCCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
GTPAHVINFSLLPHTPEDLVLIDEVLGQGHLTILSRGYGNCRIDAAARRDVWRVRYYNST
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECHHHCCCEEEEEEECCC
DMLILDTLEITSLPEVAQAAPEDIADSAARLAQVLDALA
CEEEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ASPFAIQPPLGFGPGSQSGDEEGLAYLPMPSGMRVYEPHLLDADVAGPCAAALATLAAV
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEECCCEECCCCCCHHHHHHHHHHHH
RDALARWQPGGHESFSLAGLDARNRALIDEALGEGEVAVRVTGVVPLEIQEATFAGVWRV
HHHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCCEEEEEEEEEEEEECCCHHEEEEEE
RGAESVDRIEVGAFPRGALLRAFPAEWQMDLEDIARPRPGVVNAPPILAEIAHRTSARAP
CCCCCCCEEEECCCCCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCC
GTPAHVINFSLLPHTPEDLVLIDEVLGQGHLTILSRGYGNCRIDAAARRDVWRVRYYNST
CCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECHHHCCCEEEEEEECCC
DMLILDTLEITSLPEVAQAAPEDIADSAARLAQVLDALA
CEEEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]