| Definition | Azorhizobium caulinodans ORS 571, complete genome. |
|---|---|
| Accession | NC_009937 |
| Length | 5,369,772 |
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The map label for this gene is hypF [H]
Identifier: 158422221
GI number: 158422221
Start: 663003
End: 665294
Strand: Direct
Name: hypF [H]
Synonym: AZC_0597
Alternate gene names: 158422221
Gene position: 663003-665294 (Clockwise)
Preceding gene: 158422220
Following gene: 158422222
Centisome position: 12.35
GC content: 71.47
Gene sequence:
>2292_bases ATGGAGCGCACGGGCGAAGCTGAAGAGATCACGGTGCGCGGCATCGTGCAGGGGGTGGGCTTCCGCCCCTTCGTCTGGCG CCTCGCCCGCCGGTTCGGACTCGCGGGAAGCGTCAGCAATACCGGCGATGCGGTGCGCATCCATATCCGCGGGCCTGTCG AGGCCCGCGCGGCTTTCGTCGCCGCGCTCGTGGCCGAGGCTCCGCCGCTTGCCCGCGTCGAGCAGGTGACGCGCGTCCCT GCTGCCCCGGTCGAGGGGGCAGAGTTCCACATCGCGGAGAGTGGGCTGGGGACGGTGTCCGTGGGCATCGTGCCGGATCT CGCCACCTGTCCCGCCTGCCGTTCGGAGATCGCGGACCCGAAGGCCCGGCGCTTCCGCTATGCCTTCACCAATTGCACCG ATTGCGGCCCGCGCTTCTCCATCGTGGAAGGCCTGCCCTATGACCGCCCGCGCACCACCATGCGCGCCTTCGCCCTGTGC CCCGACTGTCGGCGCGAATATGACGACCCGGCCGACCGTCGCTTCCATGCCCAGCCCATCGCCTGCCCCACCTGCGGCCC GCGCCTGCGCTTCGGGGCCTTCGAGGGAGATGCGGCGCTGGCGGCGGCTGCGAGTGCTCTGAAGGCGGGCGCGATCCTGG CAGTGAAAGGCATCGGCGGCTTCCACATCGCCTGCGATGCCACCAGCGAGGCGGTGGTGGCGGAGCTGCGCCGCCGCAAG CAGCGTCCGGCCAAGCCCCTCGCCGTGATGGTGGCTGACCTCGCCGCTGCTCGTGCGTTGGCCGAAGTGTCAGCCCTTGA GGCGGAGGCTCTTTCGGACCCGTCCGCGCCCATCGTTCTGCTGCGCCGGCACCTTGATGGACCGCTCGCGCCCTCGGTTG CGCCGGGCCAGAGCCACGTGGGGCTGATGCTGACGTATACGCCCCTGCATCACCTGCTGCTGGCGAGCGTGGGTGGGCCG CTGGTGATGACCAGCGCCAACAGGGCCAATGCCCCGCAGGTGTTCCGCGACGAAGAGGCCGAGGCTGCGCTCGCCGGCAT CGTGGACGGCCTCCTCACCCACGACCGCCCCATCGCCCGGCGGTTGGAGGATGGCGTGGTGCGCATCGCCGCCGGCGGCG TGCGGGTGATGCGTCGGGGGCGCGGGCAGGCGCCCCGCGCCCTCGACTGGCCGGCGGATTTCGAGGGGGCGCCGCCCATC CTCGCGCTCGGGAGCGATCTCAAGAGCGCTGCCTGTCTCAGCCATGGCCGCAAGGCGCTGCTGACCCATCATCTGGGCGA CCTCGATACGCCCGCCGTGGAAGACGAGTTCGCCCGCGCGCTGGCCGATATGGCGGATGTGTTCGCGCATCATCCTGAGG CGGTGGCGGTGGACCTCCATCCCGGCTACCGCGCGACGCAGTTTGGCACGGCACTGGCGGTGGAGCGGGGCCTGCCGCTG CTGGCTATTCCGCACCATCACGCCCATATCGCCGCCACTCTGGCGGAAAACGGCTGGCCGCGTCTCGGGGGGCCGGTGGT GGGCCTTGCCCTCGACGGTCTCGGCCTCGGGGACGATGGCACGCTCTGGGGCGCCGAGGTGCTCGTCTGCGATTATCGGG AGAGCCGCCGCATAGCGCGCCTCGTCCCCATTCCGCTCGCCGGCGGGGATGTTGCGAGCCGGGAGCCGTGGCGCGTGCTG CTGGCTCATCTGGACACGGCGGTGGGGGAAGATGGCTTTCCGGCGGACCTTCTGGATGGAAAGCCGCTTGCCACCGTGCG GGCCATGATCGCCAAGGGGCTCAATGCGCCACTCGCCAGTTCCGCCGGGCGGCTGTTCGATGCCATGGCGGCGCTGCTTG GCCTCGCGCCCGATCGCCTCTCCTTTGAGGGCGAGGCGGCGATGGCGCTGGAAGCCTGTGCGGGCGACATCATGGCCGCG CCCTATCCCATGGCGGTGCGGGAGGCCGACGGCCTCATGGAACTGGATCCGGCGCCGCTCTGGCGGACGGCATTGGCCGA CCGGGCAGCGGGCGTGCCCGCCCCCGCGATGGCGGCGCGATTCCATGCGGGTGTCGCCGCGGCCTTTGCCGACCTTGCCG CCTGGGTGGCAGAAGGCCATGGGCTATCCACCGTCGCTTTGTCCGGCGGCGTGTTTCAGAACGCCCGCTTGCTCGAAGAC GTGGCGGCCCGCCTGTCCGCCCATGGTCTTGCCGTGCTGCTGCCCGCCGAGGTGCCGGCCAATGACGGCGGCCTCGCCTT CGGGCAGGCGGCCATCGCGGCGGCGCGGCTGATGGCGTCAGAGGGCCGGTAA
Upstream 100 bases:
>100_bases GGTGCTCCGGTGCAGGAGGGCGAGACGACACCGCTTTCCGTGCAGCACATCGTGCGCTCCTTCGACCCCTGCATGGTCTG CACGGTGCATTGAGGCGCCC
Downstream 100 bases:
>100_bases GCGGCTTTCCGTCGCGCGTGGAAAGCGGCGTTCCGCTTTCCGCCGGTCGCACCGGTGCGAGAACGGAAAAGCCTTGATCT GGAACGCTTCCAGCCTGTGG
Product: hydrogenase maturation protein
Products: NA
Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]
Number of amino acids: Translated: 763; Mature: 763
Protein sequence:
>763_residues MERTGEAEEITVRGIVQGVGFRPFVWRLARRFGLAGSVSNTGDAVRIHIRGPVEARAAFVAALVAEAPPLARVEQVTRVP AAPVEGAEFHIAESGLGTVSVGIVPDLATCPACRSEIADPKARRFRYAFTNCTDCGPRFSIVEGLPYDRPRTTMRAFALC PDCRREYDDPADRRFHAQPIACPTCGPRLRFGAFEGDAALAAAASALKAGAILAVKGIGGFHIACDATSEAVVAELRRRK QRPAKPLAVMVADLAAARALAEVSALEAEALSDPSAPIVLLRRHLDGPLAPSVAPGQSHVGLMLTYTPLHHLLLASVGGP LVMTSANRANAPQVFRDEEAEAALAGIVDGLLTHDRPIARRLEDGVVRIAAGGVRVMRRGRGQAPRALDWPADFEGAPPI LALGSDLKSAACLSHGRKALLTHHLGDLDTPAVEDEFARALADMADVFAHHPEAVAVDLHPGYRATQFGTALAVERGLPL LAIPHHHAHIAATLAENGWPRLGGPVVGLALDGLGLGDDGTLWGAEVLVCDYRESRRIARLVPIPLAGGDVASREPWRVL LAHLDTAVGEDGFPADLLDGKPLATVRAMIAKGLNAPLASSAGRLFDAMAALLGLAPDRLSFEGEAAMALEACAGDIMAA PYPMAVREADGLMELDPAPLWRTALADRAAGVPAPAMAARFHAGVAAAFADLAAWVAEGHGLSTVALSGGVFQNARLLED VAARLSAHGLAVLLPAEVPANDGGLAFGQAAIAAARLMASEGR
Sequences:
>Translated_763_residues MERTGEAEEITVRGIVQGVGFRPFVWRLARRFGLAGSVSNTGDAVRIHIRGPVEARAAFVAALVAEAPPLARVEQVTRVP AAPVEGAEFHIAESGLGTVSVGIVPDLATCPACRSEIADPKARRFRYAFTNCTDCGPRFSIVEGLPYDRPRTTMRAFALC PDCRREYDDPADRRFHAQPIACPTCGPRLRFGAFEGDAALAAAASALKAGAILAVKGIGGFHIACDATSEAVVAELRRRK QRPAKPLAVMVADLAAARALAEVSALEAEALSDPSAPIVLLRRHLDGPLAPSVAPGQSHVGLMLTYTPLHHLLLASVGGP LVMTSANRANAPQVFRDEEAEAALAGIVDGLLTHDRPIARRLEDGVVRIAAGGVRVMRRGRGQAPRALDWPADFEGAPPI LALGSDLKSAACLSHGRKALLTHHLGDLDTPAVEDEFARALADMADVFAHHPEAVAVDLHPGYRATQFGTALAVERGLPL LAIPHHHAHIAATLAENGWPRLGGPVVGLALDGLGLGDDGTLWGAEVLVCDYRESRRIARLVPIPLAGGDVASREPWRVL LAHLDTAVGEDGFPADLLDGKPLATVRAMIAKGLNAPLASSAGRLFDAMAALLGLAPDRLSFEGEAAMALEACAGDIMAA PYPMAVREADGLMELDPAPLWRTALADRAAGVPAPAMAARFHAGVAAAFADLAAWVAEGHGLSTVALSGGVFQNARLLED VAARLSAHGLAVLLPAEVPANDGGLAFGQAAIAAARLMASEGR >Mature_763_residues MERTGEAEEITVRGIVQGVGFRPFVWRLARRFGLAGSVSNTGDAVRIHIRGPVEARAAFVAALVAEAPPLARVEQVTRVP AAPVEGAEFHIAESGLGTVSVGIVPDLATCPACRSEIADPKARRFRYAFTNCTDCGPRFSIVEGLPYDRPRTTMRAFALC PDCRREYDDPADRRFHAQPIACPTCGPRLRFGAFEGDAALAAAASALKAGAILAVKGIGGFHIACDATSEAVVAELRRRK QRPAKPLAVMVADLAAARALAEVSALEAEALSDPSAPIVLLRRHLDGPLAPSVAPGQSHVGLMLTYTPLHHLLLASVGGP LVMTSANRANAPQVFRDEEAEAALAGIVDGLLTHDRPIARRLEDGVVRIAAGGVRVMRRGRGQAPRALDWPADFEGAPPI LALGSDLKSAACLSHGRKALLTHHLGDLDTPAVEDEFARALADMADVFAHHPEAVAVDLHPGYRATQFGTALAVERGLPL LAIPHHHAHIAATLAENGWPRLGGPVVGLALDGLGLGDDGTLWGAEVLVCDYRESRRIARLVPIPLAGGDVASREPWRVL LAHLDTAVGEDGFPADLLDGKPLATVRAMIAKGLNAPLASSAGRLFDAMAALLGLAPDRLSFEGEAAMALEACAGDIMAA PYPMAVREADGLMELDPAPLWRTALADRAAGVPAPAMAARFHAGVAAAFADLAAWVAEGHGLSTVALSGGVFQNARLLED VAARLSAHGLAVLLPAEVPANDGGLAFGQAAIAAARLMASEGR
Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb
COG id: COG0068
COG function: function code O; Hydrogenase maturation factor
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 YrdC-like domain [H]
Homologues:
Organism=Escherichia coli, GI2367152, Length=770, Percent_Identity=45.8441558441558, Blast_Score=555, Evalue=1e-159,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001792 - InterPro: IPR017968 - InterPro: IPR004421 - InterPro: IPR017945 - InterPro: IPR006070 - InterPro: IPR011125 [H]
Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]
EC number: NA
Molecular weight: Translated: 79682; Mature: 79682
Theoretical pI: Translated: 6.41; Mature: 6.41
Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MERTGEAEEITVRGIVQGVGFRPFVWRLARRFGLAGSVSNTGDAVRIHIRGPVEARAAFV CCCCCCCHHEEHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHH AALVAEAPPLARVEQVTRVPAAPVEGAEFHIAESGLGTVSVGIVPDLATCPACRSEIADP HHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCC KARRFRYAFTNCTDCGPRFSIVEGLPYDRPRTTMRAFALCPDCRREYDDPADRRFHAQPI HHHHEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCHHHHHHCCCCHHCCCCCCCC ACPTCGPRLRFGAFEGDAALAAAASALKAGAILAVKGIGGFHIACDATSEAVVAELRRRK CCCCCCCCCEECCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHHHH QRPAKPLAVMVADLAAARALAEVSALEAEALSDPSAPIVLLRRHLDGPLAPSVAPGQSHV CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC GLMLTYTPLHHLLLASVGGPLVMTSANRANAPQVFRDEEAEAALAGIVDGLLTHDRPIAR EEEEEEHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCHHHH RLEDGVVRIAAGGVRVMRRGRGQAPRALDWPADFEGAPPILALGSDLKSAACLSHGRKAL HHHCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCHHHH LTHHLGDLDTPAVEDEFARALADMADVFAHHPEAVAVDLHPGYRATQFGTALAVERGLPL HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCE LAIPHHHAHIAATLAENGWPRLGGPVVGLALDGLGLGDDGTLWGAEVLVCDYRESRRIAR EEECCCCHHEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHCCCEEE LVPIPLAGGDVASREPWRVLLAHLDTAVGEDGFPADLLDGKPLATVRAMIAKGLNAPLAS EEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHCCCCCHHH SAGRLFDAMAALLGLAPDRLSFEGEAAMALEACAGDIMAAPYPMAVREADGLMELDPAPL HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCHHHCCCCCEECCCCCH WRTALADRAAGVPAPAMAARFHAGVAAAFADLAAWVAEGHGLSTVALSGGVFQNARLLED HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH VAARLSAHGLAVLLPAEVPANDGGLAFGQAAIAAARLMASEGR HHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC >Mature Secondary Structure MERTGEAEEITVRGIVQGVGFRPFVWRLARRFGLAGSVSNTGDAVRIHIRGPVEARAAFV CCCCCCCHHEEHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHHHHHHH AALVAEAPPLARVEQVTRVPAAPVEGAEFHIAESGLGTVSVGIVPDLATCPACRSEIADP HHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHHHCCCC KARRFRYAFTNCTDCGPRFSIVEGLPYDRPRTTMRAFALCPDCRREYDDPADRRFHAQPI HHHHEEEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCHHHHHHCCCCHHCCCCCCCC ACPTCGPRLRFGAFEGDAALAAAASALKAGAILAVKGIGGFHIACDATSEAVVAELRRRK CCCCCCCCCEECCCCCCHHHHHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHHHHHHHH QRPAKPLAVMVADLAAARALAEVSALEAEALSDPSAPIVLLRRHLDGPLAPSVAPGQSHV CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC GLMLTYTPLHHLLLASVGGPLVMTSANRANAPQVFRDEEAEAALAGIVDGLLTHDRPIAR EEEEEEHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCHHHH RLEDGVVRIAAGGVRVMRRGRGQAPRALDWPADFEGAPPILALGSDLKSAACLSHGRKAL HHHCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCHHHH LTHHLGDLDTPAVEDEFARALADMADVFAHHPEAVAVDLHPGYRATQFGTALAVERGLPL HHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCE LAIPHHHAHIAATLAENGWPRLGGPVVGLALDGLGLGDDGTLWGAEVLVCDYRESRRIAR EEECCCCHHEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHCCCEEE LVPIPLAGGDVASREPWRVLLAHLDTAVGEDGFPADLLDGKPLATVRAMIAKGLNAPLAS EEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHCCCCCHHH SAGRLFDAMAALLGLAPDRLSFEGEAAMALEACAGDIMAAPYPMAVREADGLMELDPAPL HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCHHHCCCCCEECCCCCH WRTALADRAAGVPAPAMAARFHAGVAAAFADLAAWVAEGHGLSTVALSGGVFQNARLLED HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHH VAARLSAHGLAVLLPAEVPANDGGLAFGQAAIAAARLMASEGR HHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190; 9492269 [H]