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The map label for this gene is hupV [C]

Identifier: 158422220

GI number: 158422220

Start: 661523

End: 662995

Strand: Direct

Name: hupV [C]

Synonym: AZC_0596

Alternate gene names: 158422220

Gene position: 661523-662995 (Clockwise)

Preceding gene: 158422219

Following gene: 158422221

Centisome position: 12.32

GC content: 71.76

Gene sequence:

>1473_bases
ATGAGGGATGCGTCAGCGAAGGACGGGACGGCCCGGCTCATCGTCGGCCCGTTCAACCGCGTGGAGGGCGATCTTGAGGT
GCGCCTTGAGGTGGCGGACGGCGCAGTGCGCGCGGCCTTCGTCTCCTCGCCGCTGTTCCGCGGCTTCGAGCGCATATTGG
AGGGGCGCGATCCCTTCGACGCGCTGGTGATCGCCCCACGCATCTGCGGCATCTGTTCGGTGTCGCAATCGCAGGCGGCG
GCGCTGGCGCTGGCGCAATGGCAGGGCCTCGCGCCCACCGCCAACGGGCGCATCGCCACCAATCTCCTCGTGGCGGCGGA
GAACGTGGCCGACCACCTCACCCATTTCCACCTCTTCTTCATGCCGGATTTCGCCCGCGCGGCCTATGCGGACCGGCCGT
GGTTCGCCGCCGCCGAGGCGCGCTTCAAGGCGGGGCGAGGCACCAGCGCGCGGCGGCTCATCGAGGCGCGGGCGACGCTC
ATGCACGTCCTCGGCCTGCTGGCGGGGCGCTGGCCGCATACGCTTGCCATCCAGCCCGGCGGCGTGACGCGCCGGGCCGA
GCCGCGCGACCAGATGCGCCTTCTGGCGACGCTAGGCGCCGTGCGTGCGACGCTGGAACAAACCCTCTATGGCGCGCCCC
TGGAGCGGATCTCCGCTCTGGCGGACCCGCGGGAGTTGGAGGCGTGGCGCGCGTTGGGCCCGGATGGCGATTTCCGCCTG
TTCCTTGAAATCGCCGCCGACCTCGATCTCACCCACCTTGGGCGGGCCTATGACCGCTTCCTGAGCTTCGGTGCCTATCC
GGGCGAGGAGGGGCCGCATTACCCGGCCGGCACCTTCGCGGACGGGGTCTTGGGCGGCGTGGACGAGGCGGCCATCGCCG
AGGACCACACCTTCGCCCGCATGGAGGATCGCGCGGCCCCGCATCACCCCTTCGCCGGCTCCACCATTCCCGATGGCTTC
GACGAGACCGGCTACACCTGGTGCAAGGCGCCGCGCCTGGCCGGCCAGCCCTTCGAGACCGGCGCGCTGGCCCGCCAGTT
CATGGACGGCCAGCCGCTCGCCCGCGCGCTGGTGGCGGCGGGCGGCGGCAATGTCTTCAGCCGTGTCATCGGCCGGTTGA
TCGAGACCGCGCGCACCCTCATCGCCATGGAGGGCTGGGTGCGGGCGCTGGAGCCTGCGGAGCCCTGGTGCGCCCATGGG
ACGAACCCGTCCGAGGGGCGGGGCGCCGGGCTGACGGAAGCGGCGCGCGGGGCTCTGGGCCACTGGATGGAGGTCCGGAA
CGGGCGCATCGCGCGCTATCAGATCATCGCACCCACCACCTGGAATTTCTCGCCCCGCGACGCGGCGGGCGTGCCCGGCC
CGCTGGAGGCGGCGCTGGTGGGTGCTCCGGTGCAGGAGGGCGAGACGACACCGCTTTCCGTGCAGCACATCGTGCGCTCC
TTCGACCCCTGCATGGTCTGCACGGTGCATTGA

Upstream 100 bases:

>100_bases
TCTCCTCGCTCGCCAAGGCGGCAACGCCCGAGCGGCTGCGGCGCAATGCGGTGTCCGACCATGTGGTGATGCCGCCCACC
GTGCGGGACATCAAGCGCCG

Downstream 100 bases:

>100_bases
GGCGCCCATGGAGCGCACGGGCGAAGCTGAAGAGATCACGGTGCGCGGCATCGTGCAGGGGGTGGGCTTCCGCCCCTTCG
TCTGGCGCCTCGCCCGCCGG

Product: HupV protein

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRDASAKDGTARLIVGPFNRVEGDLEVRLEVADGAVRAAFVSSPLFRGFERILEGRDPFDALVIAPRICGICSVSQSQAA
ALALAQWQGLAPTANGRIATNLLVAAENVADHLTHFHLFFMPDFARAAYADRPWFAAAEARFKAGRGTSARRLIEARATL
MHVLGLLAGRWPHTLAIQPGGVTRRAEPRDQMRLLATLGAVRATLEQTLYGAPLERISALADPRELEAWRALGPDGDFRL
FLEIAADLDLTHLGRAYDRFLSFGAYPGEEGPHYPAGTFADGVLGGVDEAAIAEDHTFARMEDRAAPHHPFAGSTIPDGF
DETGYTWCKAPRLAGQPFETGALARQFMDGQPLARALVAAGGGNVFSRVIGRLIETARTLIAMEGWVRALEPAEPWCAHG
TNPSEGRGAGLTEAARGALGHWMEVRNGRIARYQIIAPTTWNFSPRDAAGVPGPLEAALVGAPVQEGETTPLSVQHIVRS
FDPCMVCTVH

Sequences:

>Translated_490_residues
MRDASAKDGTARLIVGPFNRVEGDLEVRLEVADGAVRAAFVSSPLFRGFERILEGRDPFDALVIAPRICGICSVSQSQAA
ALALAQWQGLAPTANGRIATNLLVAAENVADHLTHFHLFFMPDFARAAYADRPWFAAAEARFKAGRGTSARRLIEARATL
MHVLGLLAGRWPHTLAIQPGGVTRRAEPRDQMRLLATLGAVRATLEQTLYGAPLERISALADPRELEAWRALGPDGDFRL
FLEIAADLDLTHLGRAYDRFLSFGAYPGEEGPHYPAGTFADGVLGGVDEAAIAEDHTFARMEDRAAPHHPFAGSTIPDGF
DETGYTWCKAPRLAGQPFETGALARQFMDGQPLARALVAAGGGNVFSRVIGRLIETARTLIAMEGWVRALEPAEPWCAHG
TNPSEGRGAGLTEAARGALGHWMEVRNGRIARYQIIAPTTWNFSPRDAAGVPGPLEAALVGAPVQEGETTPLSVQHIVRS
FDPCMVCTVH
>Mature_490_residues
MRDASAKDGTARLIVGPFNRVEGDLEVRLEVADGAVRAAFVSSPLFRGFERILEGRDPFDALVIAPRICGICSVSQSQAA
ALALAQWQGLAPTANGRIATNLLVAAENVADHLTHFHLFFMPDFARAAYADRPWFAAAEARFKAGRGTSARRLIEARATL
MHVLGLLAGRWPHTLAIQPGGVTRRAEPRDQMRLLATLGAVRATLEQTLYGAPLERISALADPRELEAWRALGPDGDFRL
FLEIAADLDLTHLGRAYDRFLSFGAYPGEEGPHYPAGTFADGVLGGVDEAAIAEDHTFARMEDRAAPHHPFAGSTIPDGF
DETGYTWCKAPRLAGQPFETGALARQFMDGQPLARALVAAGGGNVFSRVIGRLIETARTLIAMEGWVRALEPAEPWCAHG
TNPSEGRGAGLTEAARGALGHWMEVRNGRIARYQIIAPTTWNFSPRDAAGVPGPLEAALVGAPVQEGETTPLSVQHIVRS
FDPCMVCTVH

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=565, Percent_Identity=26.3716814159292, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787207, Length=283, Percent_Identity=32.5088339222615, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 52730; Mature: 52730

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDASAKDGTARLIVGPFNRVEGDLEVRLEVADGAVRAAFVSSPLFRGFERILEGRDPFD
CCCCCCCCCCEEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
ALVIAPRICGICSVSQSQAAALALAQWQGLAPTANGRIATNLLVAAENVADHLTHFHLFF
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEE
MPDFARAAYADRPWFAAAEARFKAGRGTSARRLIEARATLMHVLGLLAGRWPHTLAIQPG
CCCHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
GVTRRAEPRDQMRLLATLGAVRATLEQTLYGAPLERISALADPRELEAWRALGPDGDFRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCCCEEE
FLEIAADLDLTHLGRAYDRFLSFGAYPGEEGPHYPAGTFADGVLGGVDEAAIAEDHTFAR
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCHHHH
MEDRAAPHHPFAGSTIPDGFDETGYTWCKAPRLAGQPFETGALARQFMDGQPLARALVAA
HHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHC
GGGNVFSRVIGRLIETARTLIAMEGWVRALEPAEPWCAHGTNPSEGRGAGLTEAARGALG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHH
HWMEVRNGRIARYQIIAPTTWNFSPRDAAGVPGPLEAALVGAPVQEGETTPLSVQHIVRS
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHC
FDPCMVCTVH
CCCEEEEEEC
>Mature Secondary Structure
MRDASAKDGTARLIVGPFNRVEGDLEVRLEVADGAVRAAFVSSPLFRGFERILEGRDPFD
CCCCCCCCCCEEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHH
ALVIAPRICGICSVSQSQAAALALAQWQGLAPTANGRIATNLLVAAENVADHLTHFHLFF
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEE
MPDFARAAYADRPWFAAAEARFKAGRGTSARRLIEARATLMHVLGLLAGRWPHTLAIQPG
CCCHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
GVTRRAEPRDQMRLLATLGAVRATLEQTLYGAPLERISALADPRELEAWRALGPDGDFRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCCCEEE
FLEIAADLDLTHLGRAYDRFLSFGAYPGEEGPHYPAGTFADGVLGGVDEAAIAEDHTFAR
EEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHCCCHHHH
MEDRAAPHHPFAGSTIPDGFDETGYTWCKAPRLAGQPFETGALARQFMDGQPLARALVAA
HHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHC
GGGNVFSRVIGRLIETARTLIAMEGWVRALEPAEPWCAHGTNPSEGRGAGLTEAARGALG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHH
HWMEVRNGRIARYQIIAPTTWNFSPRDAAGVPGPLEAALVGAPVQEGETTPLSVQHIVRS
HHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHC
FDPCMVCTVH
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]