Click here to switch to the map view.

The map label for this gene is mmsB [H]

Identifier: 158422201

GI number: 158422201

Start: 640336

End: 641229

Strand: Direct

Name: mmsB [H]

Synonym: AZC_0577

Alternate gene names: 158422201

Gene position: 640336-641229 (Clockwise)

Preceding gene: 158422200

Following gene: 158422205

Centisome position: 11.92

GC content: 70.47

Gene sequence:

>894_bases
ATGGCCGTCATCGCCTTCATCGGGCTCGGCAACATGGGCGGGCCGATGGCCGCCAATCTGGTCAAGGCAGGGCACGCGGT
GCACGGCTTTGACCTCTCCGAGGCCGCCAAGGCGGCGGCCGCCGCGCGCGGCGTCTCGCTGGCGGTAAGTGCGGCCGAGG
CGGTGAAGGCGGCGGACGTGGTCGTCACCATGCTGCCCTCCGGCCCGCATGTGCTGGGGGCGGTCACGGGTGCGGATGGC
CTGTTCGCGGCGGCGCGGCCCGGCACGCTGTTCATCGACAGCTCAACCATCGACGTGGAGAGCGCCCGCGCCTTCCATGC
GGGGGCCGAGGCGGCCGGCATGGCCAGCCTTGATGCGCCCGTTTCCGGTGGCGTCGGCGGTGCGGAGGCGGGCACGCTCA
CCTTCATGGCGGGTGGCTCCGAGGCGGCATTCTCGGCGGCGAAGCCCCATCTTGAGCGGATGGGCAAGAAGATCGTCCAT
TGCGGCGGCGCGGGCGCGGGGCAGGCGGCGAAGATCTGCAACAACATGATCCTCGGCATCTCCATGATCGCGGTGAGCGA
GGCCTTCGTGCTGGGCGAGAAGCTGGGCCTTTCCCATCAGGCGCTGTTCGACGTGGCTTCCACCTCGTCCGGCCAGTGCT
GGTCGCTCACCACCTATTGCCCGGTGCCGGGGCCGGTGCCCACGAGCCCCGCGAACCGCGAGTATCAGCCCGGCTTTGCC
GCCGCGCTGATGCTCAAGGATCTCAAGCTCTCGCAGCAGGCGGCGGATCAGGCGGGGGCGGCCACACGGCTCGGCGCCGA
GGCGGCTGCCCTCTATTCTGCCTTCGAGGCGGAAGGCAATGCGGGCCGGGATTTCTCCGGCATCATCGAGTGGATCCGCG
CCCGCACGGCCTGA

Upstream 100 bases:

>100_bases
AGATCATGCGCGTCATCATTGCGCGCGGATTGGTGGCGCGGGGCAACTGAAGCCCGCCGCGCCCATCCAACAGAACACTG
CATCCTTAAGGGAGGACACC

Downstream 100 bases:

>100_bases
CGCGGGGATCCCTCAGGCGCTCGCCGCGTCGAGCGCGGCGATCTCCTCCGCCGATAACTGGAGATGGACGGCACGGATGA
GCCCGTCCATCTGCGCGAGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAVIAFIGLGNMGGPMAANLVKAGHAVHGFDLSEAAKAAAAARGVSLAVSAAEAVKAADVVVTMLPSGPHVLGAVTGADG
LFAAARPGTLFIDSSTIDVESARAFHAGAEAAGMASLDAPVSGGVGGAEAGTLTFMAGGSEAAFSAAKPHLERMGKKIVH
CGGAGAGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANREYQPGFA
AALMLKDLKLSQQAADQAGAATRLGAEAAALYSAFEAEGNAGRDFSGIIEWIRARTA

Sequences:

>Translated_297_residues
MAVIAFIGLGNMGGPMAANLVKAGHAVHGFDLSEAAKAAAAARGVSLAVSAAEAVKAADVVVTMLPSGPHVLGAVTGADG
LFAAARPGTLFIDSSTIDVESARAFHAGAEAAGMASLDAPVSGGVGGAEAGTLTFMAGGSEAAFSAAKPHLERMGKKIVH
CGGAGAGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANREYQPGFA
AALMLKDLKLSQQAADQAGAATRLGAEAAALYSAFEAEGNAGRDFSGIIEWIRARTA
>Mature_296_residues
AVIAFIGLGNMGGPMAANLVKAGHAVHGFDLSEAAKAAAAARGVSLAVSAAEAVKAADVVVTMLPSGPHVLGAVTGADGL
FAAARPGTLFIDSSTIDVESARAFHAGAEAAGMASLDAPVSGGVGGAEAGTLTFMAGGSEAAFSAAKPHLERMGKKIVHC
GGAGAGQAAKICNNMILGISMIAVSEAFVLGEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANREYQPGFAA
ALMLKDLKLSQQAADQAGAATRLGAEAAALYSAFEAEGNAGRDFSGIIEWIRARTA

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=43.7931034482759, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI40556376, Length=285, Percent_Identity=25.2631578947368, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI145693186, Length=285, Percent_Identity=33.6842105263158, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=34.5762711864407, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1786719, Length=294, Percent_Identity=33.6734693877551, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=28.5223367697594, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=299, Percent_Identity=42.1404682274247, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=45.2054794520548, Blast_Score=250, Evalue=8e-67,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=45.2054794520548, Blast_Score=250, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24655240, Length=64, Percent_Identity=50, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28574115, Length=291, Percent_Identity=20.9621993127148, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29527; Mature: 29396

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIAFIGLGNMGGPMAANLVKAGHAVHGFDLSEAAKAAAAARGVSLAVSAAEAVKAADV
CEEEEEEECCCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHCCHHEEEHHHHHHHHHCE
VVTMLPSGPHVLGAVTGADGLFAAARPGTLFIDSSTIDVESARAFHAGAEAAGMASLDAP
EEEECCCCCEEEEEEECCCCCEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCC
VSGGVGGAEAGTLTFMAGGSEAAFSAAKPHLERMGKKIVHCGGAGAGQAAKICNNMILGI
CCCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHH
SMIAVSEAFVLGEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANREYQPGFA
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHH
AALMLKDLKLSQQAADQAGAATRLGAEAAALYSAFEAEGNAGRDFSGIIEWIRARTA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AVIAFIGLGNMGGPMAANLVKAGHAVHGFDLSEAAKAAAAARGVSLAVSAAEAVKAADV
EEEEEEECCCCCCHHHHHHHHCCCCEECCCHHHHHHHHHHHCCHHEEEHHHHHHHHHCE
VVTMLPSGPHVLGAVTGADGLFAAARPGTLFIDSSTIDVESARAFHAGAEAAGMASLDAP
EEEECCCCCEEEEEEECCCCCEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCC
VSGGVGGAEAGTLTFMAGGSEAAFSAAKPHLERMGKKIVHCGGAGAGQAAKICNNMILGI
CCCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHH
SMIAVSEAFVLGEKLGLSHQALFDVASTSSGQCWSLTTYCPVPGPVPTSPANREYQPGFA
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHH
AALMLKDLKLSQQAADQAGAATRLGAEAAALYSAFEAEGNAGRDFSGIIEWIRARTA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]