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The map label for this gene is yefM [H]

Identifier: 158422162

GI number: 158422162

Start: 596971

End: 597243

Strand: Direct

Name: yefM [H]

Synonym: AZC_0538

Alternate gene names: 158422162

Gene position: 596971-597243 (Clockwise)

Preceding gene: 158422161

Following gene: 158422163

Centisome position: 11.12

GC content: 66.3

Gene sequence:

>273_bases
ATGATCCGACCCCGTGCCATGGACGTGCTGAGCTATTCCGACACCCGCGCCAACCTCAAAGAGGTGATGGACCGGGTGGT
GGAGGACCATGCGCCGGTAGTCATCACCCGCCAGAAGGCGGAGGCGGTGGTGATGGTCTCCTTGAGTGACTGGAGCGCCA
TGGAGGAGACGCTGCATCTGCTCTCCTCCCCCGCCAATGCGGCGCGGCTGCGCGAAGGCGTGGCCGAGCTTGAGGCCGGT
GGCGGCAGCGAACGCGACCTTGTGGTGCCGTGA

Upstream 100 bases:

>100_bases
CTGGTGCGCGACAATTACGAGAAGTGAGCGCGTGTCCCCGGCCGCTCCGGCGGCCGGGGTTGGTCTTGAGTTGTACGGAT
TTTCGTACATATAATCGCAT

Downstream 100 bases:

>100_bases
AGCTGGTGTTCGCCACCCGCGCCTGGGAGGATTATCTCCATTGGGTGGATGCGGACCGCAACGTGCTGGAGCGGCTGAAC
GCCTTGATCGAGGAATGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MIRPRAMDVLSYSDTRANLKEVMDRVVEDHAPVVITRQKAEAVVMVSLSDWSAMEETLHLLSSPANAARLREGVAELEAG
GGSERDLVVP

Sequences:

>Translated_90_residues
MIRPRAMDVLSYSDTRANLKEVMDRVVEDHAPVVITRQKAEAVVMVSLSDWSAMEETLHLLSSPANAARLREGVAELEAG
GGSERDLVVP
>Mature_90_residues
MIRPRAMDVLSYSDTRANLKEVMDRVVEDHAPVVITRQKAEAVVMVSLSDWSAMEETLHLLSSPANAARLREGVAELEAG
GGSERDLVVP

Specific function: Part of a toxin-antitoxin (TA) module. Antitoxin that counteracts the effect of the YoeB mRNA interferase. YefM binds to the promoter region of the yefM-yeoB operon to repress transcription, YeoB acts as a corepressor [H]

COG id: COG2161

COG function: function code D; Antitoxin of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YefM antitoxin family [H]

Homologues:

Organism=Escherichia coli, GI87082026, Length=82, Percent_Identity=48.780487804878, Blast_Score=92, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006442 [H]

Pfam domain/function: PF02604 PhdYeFM [H]

EC number: NA

Molecular weight: Translated: 9845; Mature: 9845

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPRAMDVLSYSDTRANLKEVMDRVVEDHAPVVITRQKAEAVVMVSLSDWSAMEETLHL
CCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHHHHHHHH
LSSPANAARLREGVAELEAGGGSERDLVVP
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIRPRAMDVLSYSDTRANLKEVMDRVVEDHAPVVITRQKAEAVVMVSLSDWSAMEETLHL
CCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCHHHHHHHHHH
LSSPANAARLREGVAELEAGGGSERDLVVP
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]