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The map label for this gene is cfa [H]

Identifier: 158422153

GI number: 158422153

Start: 585990

End: 587249

Strand: Direct

Name: cfa [H]

Synonym: AZC_0529

Alternate gene names: 158422153

Gene position: 585990-587249 (Clockwise)

Preceding gene: 158422152

Following gene: 158422154

Centisome position: 10.91

GC content: 65.24

Gene sequence:

>1260_bases
ATGGAGGCCTTGCTTGAGCGTGTTTTGAAGCGCGTGATGACCCGTGGAAGTCTCGGCGTCACCACCGCTACCGGCCGGAC
CTATGTGGCCGGCACGGGTGATCCGAAGGTTCATATCCGTTTCACCACAAAGTCGGCCGAACGCGCGGTGCTGTTCGATC
CGGAACTCGAGATCGGCGAGGCGATCACCGACGGCACGCTGGTCATCGAGAAGGGCAGCATCACCGATTTTCTGGACCTC
GTGATGGCCCAACCCGGCGTCACCACGCCGACCATCGCCTCGCGCCTGATGCTGCTGGCCCGCATGGCCGGACGCCGCAT
CGCCCAGTTCAATCCGCGCGGCCGCTCCCAGCGCAACGTCGCGCACCATTACGATCTCGACGGACGCCTGTATTCGCTTT
TCCTTGATTCAGACCGCCAATATTCCTGCGCCTATTTCGAGCATCCGGACCAGTCTCTGGACGATGCCCAGCTTGCCAAG
AAGCGCCATCTCGCCGCCAAGCTGATGCTGGACCGGCCCGGCCTGAAGCTGCTCGACATCGGCTGCGGCTGGGGCGGCCT
CGGGCTCTACATGTCGGAGGTGGGCGGGGCCGATGTCACCGGCGTCACGCTCTCCAAGGAGCAGCTTGCGGTGGCCCAGG
GCCGGGTGAAGGAGCGCGGCCTCGACCGGCATGTGGACTTCCGCCTGCAGGACTATCGTGATGTGCCGGAGACGTTCGAC
CGCATCGTCTCGGTGGGCATGTTCGAGCACGTGGGCGTCGCCCATTACGGCACCTTCTTCCGGAAGGCGCATGACCTCCT
CAAGGAGGATGGCGTGATGATGCTGCACGCCATCGGCCGGCAGGAGCCGCCCGGCGTCACCAATCCCTGGATCGCCAAAT
ACATCTTCCCCGGCGGCTATATTCCCGCGCTCTCCGAAGTGCTCCCGCACATCGAGAAGGCGGGTCTCATCGTCACCGAC
ATCGAGATCCTGCGCCTGCATTATGCCGAAACGCTCAAGGCCTGGCGGGAGCGCTTCATGGCCCACCGGGAAGAGGCCAA
GCGCCTGACGGACGAGCGCTTCTGCCGCCTGTGGGAGTTTTATCTGGCGGGTTCGGAGATGGCCTTCCGCCACCAGAACA
TGATGGTGTTCCAGATCCAGCTGGCCCGCCGGCAGGAGGCCGTGCCGATCACCCGCGACTATCTCGCGGCGGCGGAAGAG
CGGCTGCGGGCGGCGGAGTTCGCCGTGCACGCGCCGCCGCTCCGGCTCGCCGGCGAATAG

Upstream 100 bases:

>100_bases
GCTGCTCTGGCCGCCATGGGGGCTGCCGAGCGACGTCCATTCCGCCGGGAGGGGAGGCCCGGGTCAGGGAGGCGGGCACA
TTTCAGATGGAGGCTGTGCA

Downstream 100 bases:

>100_bases
CGGCGGTACCCACCGGTTGCCCACAGCCCTGTGGGCAACCGGAAATCCAGGCGCCGTGCGGCCCGCAAGCGTGGAGGCCG
GCGCATCCCCACAATTCTTC

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MEALLERVLKRVMTRGSLGVTTATGRTYVAGTGDPKVHIRFTTKSAERAVLFDPELEIGEAITDGTLVIEKGSITDFLDL
VMAQPGVTTPTIASRLMLLARMAGRRIAQFNPRGRSQRNVAHHYDLDGRLYSLFLDSDRQYSCAYFEHPDQSLDDAQLAK
KRHLAAKLMLDRPGLKLLDIGCGWGGLGLYMSEVGGADVTGVTLSKEQLAVAQGRVKERGLDRHVDFRLQDYRDVPETFD
RIVSVGMFEHVGVAHYGTFFRKAHDLLKEDGVMMLHAIGRQEPPGVTNPWIAKYIFPGGYIPALSEVLPHIEKAGLIVTD
IEILRLHYAETLKAWRERFMAHREEAKRLTDERFCRLWEFYLAGSEMAFRHQNMMVFQIQLARRQEAVPITRDYLAAAEE
RLRAAEFAVHAPPLRLAGE

Sequences:

>Translated_419_residues
MEALLERVLKRVMTRGSLGVTTATGRTYVAGTGDPKVHIRFTTKSAERAVLFDPELEIGEAITDGTLVIEKGSITDFLDL
VMAQPGVTTPTIASRLMLLARMAGRRIAQFNPRGRSQRNVAHHYDLDGRLYSLFLDSDRQYSCAYFEHPDQSLDDAQLAK
KRHLAAKLMLDRPGLKLLDIGCGWGGLGLYMSEVGGADVTGVTLSKEQLAVAQGRVKERGLDRHVDFRLQDYRDVPETFD
RIVSVGMFEHVGVAHYGTFFRKAHDLLKEDGVMMLHAIGRQEPPGVTNPWIAKYIFPGGYIPALSEVLPHIEKAGLIVTD
IEILRLHYAETLKAWRERFMAHREEAKRLTDERFCRLWEFYLAGSEMAFRHQNMMVFQIQLARRQEAVPITRDYLAAAEE
RLRAAEFAVHAPPLRLAGE
>Mature_419_residues
MEALLERVLKRVMTRGSLGVTTATGRTYVAGTGDPKVHIRFTTKSAERAVLFDPELEIGEAITDGTLVIEKGSITDFLDL
VMAQPGVTTPTIASRLMLLARMAGRRIAQFNPRGRSQRNVAHHYDLDGRLYSLFLDSDRQYSCAYFEHPDQSLDDAQLAK
KRHLAAKLMLDRPGLKLLDIGCGWGGLGLYMSEVGGADVTGVTLSKEQLAVAQGRVKERGLDRHVDFRLQDYRDVPETFD
RIVSVGMFEHVGVAHYGTFFRKAHDLLKEDGVMMLHAIGRQEPPGVTNPWIAKYIFPGGYIPALSEVLPHIEKAGLIVTD
IEILRLHYAETLKAWRERFMAHREEAKRLTDERFCRLWEFYLAGSEMAFRHQNMMVFQIQLARRQEAVPITRDYLAAAEE
RLRAAEFAVHAPPLRLAGE

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=361, Percent_Identity=37.1191135734072, Blast_Score=217, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI133904226, Length=369, Percent_Identity=29.810298102981, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 47245; Mature: 47245

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEALLERVLKRVMTRGSLGVTTATGRTYVAGTGDPKVHIRFTTKSAERAVLFDPELEIGE
CHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCC
AITDGTLVIEKGSITDFLDLVMAQPGVTTPTIASRLMLLARMAGRRIAQFNPRGRSQRNV
EECCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
AHHYDLDGRLYSLFLDSDRQYSCAYFEHPDQSLDDAQLAKKRHLAAKLMLDRPGLKLLDI
CCEECCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
GCGWGGLGLYMSEVGGADVTGVTLSKEQLAVAQGRVKERGLDRHVDFRLQDYRDVPETFD
CCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHHH
RIVSVGMFEHVGVAHYGTFFRKAHDLLKEDGVMMLHAIGRQEPPGVTNPWIAKYIFPGGY
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEHHCCCCCCCCCCCHHHHEECCCCC
IPALSEVLPHIEKAGLIVTDIEILRLHYAETLKAWRERFMAHREEAKRLTDERFCRLWEF
HHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAGSEMAFRHQNMMVFQIQLARRQEAVPITRDYLAAAEERLRAAEFAVHAPPLRLAGE
HHCCCHHHHHHCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEALLERVLKRVMTRGSLGVTTATGRTYVAGTGDPKVHIRFTTKSAERAVLFDPELEIGE
CHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCCEEEEEEECCCCCEEEEECCCCCCCC
AITDGTLVIEKGSITDFLDLVMAQPGVTTPTIASRLMLLARMAGRRIAQFNPRGRSQRNV
EECCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
AHHYDLDGRLYSLFLDSDRQYSCAYFEHPDQSLDDAQLAKKRHLAAKLMLDRPGLKLLDI
CCEECCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
GCGWGGLGLYMSEVGGADVTGVTLSKEQLAVAQGRVKERGLDRHVDFRLQDYRDVPETFD
CCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHHH
RIVSVGMFEHVGVAHYGTFFRKAHDLLKEDGVMMLHAIGRQEPPGVTNPWIAKYIFPGGY
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEEHHCCCCCCCCCCCHHHHEECCCCC
IPALSEVLPHIEKAGLIVTDIEILRLHYAETLKAWRERFMAHREEAKRLTDERFCRLWEF
HHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAGSEMAFRHQNMMVFQIQLARRQEAVPITRDYLAAAEERLRAAEFAVHAPPLRLAGE
HHCCCHHHHHHCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]