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The map label for this gene is tolB [H]

Identifier: 158422148

GI number: 158422148

Start: 578761

End: 580113

Strand: Direct

Name: tolB [H]

Synonym: AZC_0524

Alternate gene names: 158422148

Gene position: 578761-580113 (Clockwise)

Preceding gene: 158422147

Following gene: 158422149

Centisome position: 10.78

GC content: 67.92

Gene sequence:

>1353_bases
ATGATCCATCTTCCGCGCCTGCTGACCGATCGCCTCGATCCACGCCTCTCGCGCCGGTCCGTCCTCGTCGGCGGCGCGGC
GGCGGCCGGGGCTTCGCTCCTCGGCGCCCCGGCGGCGCAGGCGGCGCTGAAGCTGGAGATTACCTCGGGCACGCCCCAGC
CCATTCCGATCGCCGTCACCGACTTCATCGGCGAGGGCGATCAGGGGCGGGCGATCAGCGGCGTCATCACGGCGGATCTC
AAGCGTTCCGGCCTGTTCGCGCCCATCGATCCCAAGGCCTTCGTGGAGCAGATCAGCAATCCCGATCTCGCCCCGCGCTT
CCAGGACTGGCGCGTCATCAATGCCCAGGGCCTTGTGACCGGTCGCGTCACCCGCCAGGGCGACGGGCGTCTGAAGGCGG
AATTCCGTCTGTGGGACGTGTTCGCCGGCCAGCAGCTCATGGGCCAGCAGTATTTCACGCAGCCGGAGAACTGGCGGCGT
GTCGCCCACATCATCGCCGACGCCATCTATGAGCGTCTTACCGGCGAGAAGGGCTATTTCGACACCCGCATCGTGTTCGT
GGATGAGAGCGGCGCCAAGGACCGGCGCGTGAAGCGGCTCGCCATCATGGACCAGGACGGCGCCAACGTGACCTATCTCA
CGCGCGGCGATGCCCTTGTCCTGACCCCCCGCTTCTCCCCGACCAGCCAGGAAATCACCTACATGTCCTATGCGCAGGGG
GAGCCGCGCGTCTTCCTGCTGAACATCGAGACCGGCCAGCGCGAGATCGTCGGCAACTTCCCCGGCATGAGCTTCGCTCC
GCGCTTCTCGCCGGACGGCCAGCGGGTGATCATGAGCCTCCAGCAGGGGGGCAATGCCAACATCTTCGTGATGGACCTGC
GCTCCAAGGCCACCACCCGCCTCACCGACACGCCGGCCATCGACACCGCGCCCTGCTATTCGCCGGACGGCCGGCAGATC
TGCTTCGAGAGCGATCGCGGCGGCTCCTCGCAGATCTATGTGATGAATGCGGACGGTTCGGGCCAGCGCCGCATCTCTTT
CGGCAACGCCCGCTACAACACGCCGGTCTGGTCGCCGCGCGGGGACGTGATCGCCTTCACCCGCCAGGCCGAGGGCAAGT
TCGCCATCGGCGTGATGCGGCCGGACGGGCAGGGCGAGCGCGTGCTGACCGAGGGCTATCATAATGAAGGCCCCACTTTC
GCGCCCAACGGCCGCGTCATCATGTTCTTCCGCGACCCGCCCGGCGGCGGCGGGCCGAACCTCTTCACCATCGACGTGAC
CGGCTACAACGAGCAGCGCGTGCCCACCCCGAGCTACGGCTCGGACCCGGCCTGGTCGCCCCTGCGCTCCTGA

Upstream 100 bases:

>100_bases
CCTTCCTTCCGGCCAACAAATATGCGGTCTGGAAGGAGATCGAGATGGGCTTCAGCCTGAAGGACATGCTCGGCCGGGCC
CACTAATCGAAGAGACTGCC

Downstream 100 bases:

>100_bases
GCGGCAGGCGGTGTGGCGCGCCGGCCACACGGGCCGCCGCCCCGGTGCGTTCATGCCTTTGCCATGATGGCGCCACGGGT
GGCGCCCACTCAAAGGCCCG

Product: twin-arginine translocation pathway signal precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MIHLPRLLTDRLDPRLSRRSVLVGGAAAAGASLLGAPAAQAALKLEITSGTPQPIPIAVTDFIGEGDQGRAISGVITADL
KRSGLFAPIDPKAFVEQISNPDLAPRFQDWRVINAQGLVTGRVTRQGDGRLKAEFRLWDVFAGQQLMGQQYFTQPENWRR
VAHIIADAIYERLTGEKGYFDTRIVFVDESGAKDRRVKRLAIMDQDGANVTYLTRGDALVLTPRFSPTSQEITYMSYAQG
EPRVFLLNIETGQREIVGNFPGMSFAPRFSPDGQRVIMSLQQGGNANIFVMDLRSKATTRLTDTPAIDTAPCYSPDGRQI
CFESDRGGSSQIYVMNADGSGQRRISFGNARYNTPVWSPRGDVIAFTRQAEGKFAIGVMRPDGQGERVLTEGYHNEGPTF
APNGRVIMFFRDPPGGGGPNLFTIDVTGYNEQRVPTPSYGSDPAWSPLRS

Sequences:

>Translated_450_residues
MIHLPRLLTDRLDPRLSRRSVLVGGAAAAGASLLGAPAAQAALKLEITSGTPQPIPIAVTDFIGEGDQGRAISGVITADL
KRSGLFAPIDPKAFVEQISNPDLAPRFQDWRVINAQGLVTGRVTRQGDGRLKAEFRLWDVFAGQQLMGQQYFTQPENWRR
VAHIIADAIYERLTGEKGYFDTRIVFVDESGAKDRRVKRLAIMDQDGANVTYLTRGDALVLTPRFSPTSQEITYMSYAQG
EPRVFLLNIETGQREIVGNFPGMSFAPRFSPDGQRVIMSLQQGGNANIFVMDLRSKATTRLTDTPAIDTAPCYSPDGRQI
CFESDRGGSSQIYVMNADGSGQRRISFGNARYNTPVWSPRGDVIAFTRQAEGKFAIGVMRPDGQGERVLTEGYHNEGPTF
APNGRVIMFFRDPPGGGGPNLFTIDVTGYNEQRVPTPSYGSDPAWSPLRS
>Mature_450_residues
MIHLPRLLTDRLDPRLSRRSVLVGGAAAAGASLLGAPAAQAALKLEITSGTPQPIPIAVTDFIGEGDQGRAISGVITADL
KRSGLFAPIDPKAFVEQISNPDLAPRFQDWRVINAQGLVTGRVTRQGDGRLKAEFRLWDVFAGQQLMGQQYFTQPENWRR
VAHIIADAIYERLTGEKGYFDTRIVFVDESGAKDRRVKRLAIMDQDGANVTYLTRGDALVLTPRFSPTSQEITYMSYAQG
EPRVFLLNIETGQREIVGNFPGMSFAPRFSPDGQRVIMSLQQGGNANIFVMDLRSKATTRLTDTPAIDTAPCYSPDGRQI
CFESDRGGSSQIYVMNADGSGQRRISFGNARYNTPVWSPRGDVIAFTRQAEGKFAIGVMRPDGQGERVLTEGYHNEGPTF
APNGRVIMFFRDPPGGGGPNLFTIDVTGYNEQRVPTPSYGSDPAWSPLRS

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=427, Percent_Identity=29.7423887587822, Blast_Score=179, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR006311
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 49309; Mature: 49309

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHLPRLLTDRLDPRLSRRSVLVGGAAAAGASLLGAPAAQAALKLEITSGTPQPIPIAVT
CCCCCHHHHHHCCCCCCCCEEEEECCHHCCHHHHCCCCCCEEEEEEEECCCCCCCEEEEE
DFIGEGDQGRAISGVITADLKRSGLFAPIDPKAFVEQISNPDLAPRFQDWRVINAQGLVT
EECCCCCCCCEEEEEEEECHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCEEE
GRVTRQGDGRLKAEFRLWDVFAGQQLMGQQYFTQPENWRRVAHIIADAIYERLTGEKGYF
EEEEECCCCEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
DTRIVFVDESGAKDRRVKRLAIMDQDGANVTYLTRGDALVLTPRFSPTSQEITYMSYAQG
EEEEEEEECCCCCCHHEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCCEEEEEECCC
EPRVFLLNIETGQREIVGNFPGMSFAPRFSPDGQRVIMSLQQGGNANIFVMDLRSKATTR
CCEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCCEEEEECCCCCCEE
LTDTPAIDTAPCYSPDGRQICFESDRGGSSQIYVMNADGSGQRRISFGNARYNTPVWSPR
ECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCCEECCCCCCCC
GDVIAFTRQAEGKFAIGVMRPDGQGERVLTEGYHNEGPTFAPNGRVIMFFRDPPGGGGPN
CCEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCCCCEECCCCCEEEEEECCCCCCCCC
LFTIDVTGYNEQRVPTPSYGSDPAWSPLRS
EEEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIHLPRLLTDRLDPRLSRRSVLVGGAAAAGASLLGAPAAQAALKLEITSGTPQPIPIAVT
CCCCCHHHHHHCCCCCCCCEEEEECCHHCCHHHHCCCCCCEEEEEEEECCCCCCCEEEEE
DFIGEGDQGRAISGVITADLKRSGLFAPIDPKAFVEQISNPDLAPRFQDWRVINAQGLVT
EECCCCCCCCEEEEEEEECHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCEEE
GRVTRQGDGRLKAEFRLWDVFAGQQLMGQQYFTQPENWRRVAHIIADAIYERLTGEKGYF
EEEEECCCCEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
DTRIVFVDESGAKDRRVKRLAIMDQDGANVTYLTRGDALVLTPRFSPTSQEITYMSYAQG
EEEEEEEECCCCCCHHEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCCEEEEEECCC
EPRVFLLNIETGQREIVGNFPGMSFAPRFSPDGQRVIMSLQQGGNANIFVMDLRSKATTR
CCEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCCEEEEECCCCCCEE
LTDTPAIDTAPCYSPDGRQICFESDRGGSSQIYVMNADGSGQRRISFGNARYNTPVWSPR
ECCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEECCCEECCCCCCCC
GDVIAFTRQAEGKFAIGVMRPDGQGERVLTEGYHNEGPTFAPNGRVIMFFRDPPGGGGPN
CCEEEEEEECCCEEEEEEECCCCCCCEEEECCCCCCCCEECCCCCEEEEEECCCCCCCCC
LFTIDVTGYNEQRVPTPSYGSDPAWSPLRS
EEEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA