Click here to switch to the map view.

The map label for this gene is pgk

Identifier: 158422126

GI number: 158422126

Start: 559584

End: 560774

Strand: Reverse

Name: pgk

Synonym: AZC_0502

Alternate gene names: 158422126

Gene position: 560774-559584 (Counterclockwise)

Preceding gene: 158422127

Following gene: 158422125

Centisome position: 10.44

GC content: 70.95

Gene sequence:

>1191_bases
ATGCCGACTTTCCGCACCCTCGATGAGGCGGACCTCGCCGGCAAGCGGGTCCTCGTCCGTGTCGACCTCAACGTCCCCAT
GGACGAAGGCCGCGTCACCGACGACACCCGCATCCAGGCCATCCTGCCCACCATCCGCGCCATCACGGAGAAGGGCGGAA
AGGCCATCCTGCTCTCCCACTTCGGACGCCCGAAAGGCCGGGACGCGAGCCAGTCGCTCGCCCCGGTCGCGGCTGCCGTC
GGGGAGCGCCTCGGCGCCCCCGTGGCGTTCGCCGGCGACTGCATCGGCTCGGATGCCGAGGCCGCCGTCTCCACCCTCGC
CCCCGGCGCCGTCCTGGTTCTGGAGAACACCCGCTTCCACGCGGGCGAGGAGAAGAACGCCGCCGATTTCGTCCAGCAGC
TCGCGGCCCTCGGCGATATCTATGTGAACGACGCCTTCTCGGCGGCCCACCGCGCCCATGCCTCCACCGAGGGGCTGGCG
CACAAGCTGCCCGCCTTCGCCGGCCGCAGCATGCAGAAGGAGCTGGAGGCGCTCGCCAAGGCCCTCGAGGCTCCGCAGCG
GCCGGTTCTGGCCGTGGTCGGCGGCGCCAAGGTGTCGAGCAAGCTCGAACTCCTCGGCAATCTCACCGCCAAGGTGGACA
TCCTCGTCATCGGCGGCGGCATGGCCAACACCTTCCTCGCCGCCAAGGGCGTGAAGGTGGGCAAGTCGCTGTGCGAGCAC
GACCTCGCCGACACCGCCCGCCAGATCATCGCCACCGCCGCCAGCCATGGCTGCGAGATCGTGCTGCCGGTGGACGCGGT
CGTCACCAAGACCTTCGCCGCCCATGCGGATCACCGCGTGGTGCCGGTGGACCAGGTGGCCGAGGACGAGATGATCCTCG
ATGCCGGCCCGGCCACCGTGGCGCTGGTGAGGGAGAAGCTGAAGGGCGCGAAGACCGTGGTCTGGAACGGCCCGTTCGGC
GCCTTCGAGCTGCCGCCCTTCGACGCCGCGACGGTTGCCGTGGCGAAGGCCGTGGCGGAGGCCACCAAGGCCGGCGCGCT
GCTGTCCGTCGCGGGTGGCGGCGACACGGTGGCGGCGCTCAACCATGCCGGCGCGGCCGGGGACTTCTCCTATGTCTCCA
CCGCCGGCGGTGCCTTCCTGGAATGGCTGGAAGGCAAGCAGTTGCCGGGGGTCGAAGCGCTGCGGCGCTGA

Upstream 100 bases:

>100_bases
CTGGTATGACAACGAGTGGGGCTTCTCCAACCGCATGTCCGACACCGCCGTCGCCCTGGCGAAGCTCGGTTGATCGGTTA
AAGCGAGGGAGAGCAGCATC

Downstream 100 bases:

>100_bases
GACCTCGGTGTTATACTCAACCCATAAAGTGCAGGCATCAGCCGCAGGGGCGGCTCCAAGGGGATAGGCAGACCATGAAC
GCTGAACTCGAGACGATCGC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPTFRTLDEADLAGKRVLVRVDLNVPMDEGRVTDDTRIQAILPTIRAITEKGGKAILLSHFGRPKGRDASQSLAPVAAAV
GERLGAPVAFAGDCIGSDAEAAVSTLAPGAVLVLENTRFHAGEEKNAADFVQQLAALGDIYVNDAFSAAHRAHASTEGLA
HKLPAFAGRSMQKELEALAKALEAPQRPVLAVVGGAKVSSKLELLGNLTAKVDILVIGGGMANTFLAAKGVKVGKSLCEH
DLADTARQIIATAASHGCEIVLPVDAVVTKTFAAHADHRVVPVDQVAEDEMILDAGPATVALVREKLKGAKTVVWNGPFG
AFELPPFDAATVAVAKAVAEATKAGALLSVAGGGDTVAALNHAGAAGDFSYVSTAGGAFLEWLEGKQLPGVEALRR

Sequences:

>Translated_396_residues
MPTFRTLDEADLAGKRVLVRVDLNVPMDEGRVTDDTRIQAILPTIRAITEKGGKAILLSHFGRPKGRDASQSLAPVAAAV
GERLGAPVAFAGDCIGSDAEAAVSTLAPGAVLVLENTRFHAGEEKNAADFVQQLAALGDIYVNDAFSAAHRAHASTEGLA
HKLPAFAGRSMQKELEALAKALEAPQRPVLAVVGGAKVSSKLELLGNLTAKVDILVIGGGMANTFLAAKGVKVGKSLCEH
DLADTARQIIATAASHGCEIVLPVDAVVTKTFAAHADHRVVPVDQVAEDEMILDAGPATVALVREKLKGAKTVVWNGPFG
AFELPPFDAATVAVAKAVAEATKAGALLSVAGGGDTVAALNHAGAAGDFSYVSTAGGAFLEWLEGKQLPGVEALRR
>Mature_395_residues
PTFRTLDEADLAGKRVLVRVDLNVPMDEGRVTDDTRIQAILPTIRAITEKGGKAILLSHFGRPKGRDASQSLAPVAAAVG
ERLGAPVAFAGDCIGSDAEAAVSTLAPGAVLVLENTRFHAGEEKNAADFVQQLAALGDIYVNDAFSAAHRAHASTEGLAH
KLPAFAGRSMQKELEALAKALEAPQRPVLAVVGGAKVSSKLELLGNLTAKVDILVIGGGMANTFLAAKGVKVGKSLCEHD
LADTARQIIATAASHGCEIVLPVDAVVTKTFAAHADHRVVPVDQVAEDEMILDAGPATVALVREKLKGAKTVVWNGPFGA
FELPPFDAATVAVAKAVAEATKAGALLSVAGGGDTVAALNHAGAAGDFSYVSTAGGAFLEWLEGKQLPGVEALRR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=42.5790754257908, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=42.1951219512195, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1789294, Length=394, Percent_Identity=45.4314720812183, Blast_Score=312, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=44.2260442260442, Blast_Score=318, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6319857, Length=408, Percent_Identity=41.6666666666667, Blast_Score=292, Evalue=6e-80,
Organism=Drosophila melanogaster, GI17136394, Length=408, Percent_Identity=42.8921568627451, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24581203, Length=410, Percent_Identity=41.7073170731707, Blast_Score=282, Evalue=3e-76,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_AZOC5 (A8IM75)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523418.1
- ProteinModelPortal:   A8IM75
- SMR:   A8IM75
- GeneID:   5688336
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0502
- HOGENOM:   HBG453500
- OMA:   SESHATH
- ProtClustDB:   CLSK979906
- BioCyc:   ACAU438753:AZC_0502-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 40771; Mature: 40640

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTFRTLDEADLAGKRVLVRVDLNVPMDEGRVTDDTRIQAILPTIRAITEKGGKAILLSH
CCCCCCCCHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
FGRPKGRDASQSLAPVAAAVGERLGAPVAFAGDCIGSDAEAAVSTLAPGAVLVLENTRFH
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCC
AGEEKNAADFVQQLAALGDIYVNDAFSAAHRAHASTEGLAHKLPAFAGRSMQKELEALAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHH
ALEAPQRPVLAVVGGAKVSSKLELLGNLTAKVDILVIGGGMANTFLAAKGVKVGKSLCEH
HHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHH
DLADTARQIIATAASHGCEIVLPVDAVVTKTFAAHADHRVVPVDQVAEDEMILDAGPATV
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCEECHHHHCCCCEEEECCCHHH
ALVREKLKGAKTVVWNGPFGAFELPPFDAATVAVAKAVAEATKAGALLSVAGGGDTVAAL
HHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEHH
NHAGAAGDFSYVSTAGGAFLEWLEGKQLPGVEALRR
HCCCCCCCCHHHHHCCHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
PTFRTLDEADLAGKRVLVRVDLNVPMDEGRVTDDTRIQAILPTIRAITEKGGKAILLSH
CCCCCCCHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
FGRPKGRDASQSLAPVAAAVGERLGAPVAFAGDCIGSDAEAAVSTLAPGAVLVLENTRFH
CCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCC
AGEEKNAADFVQQLAALGDIYVNDAFSAAHRAHASTEGLAHKLPAFAGRSMQKELEALAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHH
ALEAPQRPVLAVVGGAKVSSKLELLGNLTAKVDILVIGGGMANTFLAAKGVKVGKSLCEH
HHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHH
DLADTARQIIATAASHGCEIVLPVDAVVTKTFAAHADHRVVPVDQVAEDEMILDAGPATV
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCCEECHHHHCCCCEEEECCCHHH
ALVREKLKGAKTVVWNGPFGAFELPPFDAATVAVAKAVAEATKAGALLSVAGGGDTVAAL
HHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEHH
NHAGAAGDFSYVSTAGGAFLEWLEGKQLPGVEALRR
HCCCCCCCCHHHHHCCHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA