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The map label for this gene is otsA [H]

Identifier: 158422106

GI number: 158422106

Start: 539221

End: 540696

Strand: Reverse

Name: otsA [H]

Synonym: AZC_0482

Alternate gene names: 158422106

Gene position: 540696-539221 (Counterclockwise)

Preceding gene: 158422107

Following gene: 158422105

Centisome position: 10.07

GC content: 65.04

Gene sequence:

>1476_bases
GTGTCGCGCTTGATCGTCGTCTCCAATCGCGTTGCCGTCCCCGAGGCAGGCAAGGTCTCCGCCGGCGGACTGGCCGTCGC
CGTGAATGCGGCGCTCAAGGAGCGCTCGGGCATCTGGTTCGGCTGGAGCGGCAAGATCACCGAGCAGCCCTCCGACCAGC
CCGCGATTTCCATGCGGAACGGCGTGAAATATGCCGTGGTGGACCTCAAGGAGGATGACTACCAGGAATATTACAACGGC
TTCGCCAACCGGGTGCTGTGGCCGATCCTGCATTACCGCGTGGATCTGGCGGAGTTCGTGCGCTCGGACCTGTCCGGCTA
CATCCGGGTCAACAAAAACTTCGCCGAGCACGTCTCCAAGATCATCGAGGAAGACGACATCATCTGGGTCCACGACTATC
ACATGATGCCGCTGGCCCGGTATCTGCGGGAGCGCGGGCACAAGAACCGCATCGGCTTCTTTTTGCACATCCCCCTGCCG
CCGCCCGACCTCATCCAGGCCCTGCCGCGACACGGCGAGATCGTCGGCTCGCTGGTGCATTACGATCTCGTCGGCTTCCA
GACCGAGAGCGACAGGGACAATTTCGGCCGCTATCTGGAAGGCCGCGGGGCACGCCCCGGCAATGACGGCACCTCCTATG
AGATCGCCGGCCGCCGTGTGCGCATCGGCGCCTTCCCCGTCGCCATCGAGACGGAGAGCTTCGCCCGCCGCGCCCGCCAC
GCCATGCGCACCAATTTCATGAAGGATTTCCGTGCCAGCCTCACCGGCAAGAAGATGGTGCTGGGGGTGGACAGGCTCGA
TTATTCCAAGGGCATCCCGAACCGCCTGGAAGCCTTCGAGACGCTGCTGCGCACCGCCCCGGAATGGCACGAGCGCGTGA
CCTTCGTGCAGATCACGCCCAAGAGCCGGACCTCCGTGCCGGAATATGCGGAGATGGACCGCTATGTCTCCACCAAGGCG
GGACAGATCAATGGCGAATTCGGCGACGTCTCGTGGACGCCGCTGCGCTATGTGAACAAGACCTATTCCCGCTCAGCCCT
CGCCGGCCTCTACCGGGCAGCGGACGTGGCCATGGTGACGCCGCTGCGCGACGGCATGAACCTCGTCGCCAAGGAATTCG
TAGCCGCGCAGGATCCGGAGGACCCCGGCGTGCTGCTGCTCTCGCAGTTTGCCGGGGCGGCGGCCGAGATGGGCGGCGGT
GCGCTCATGGTGAACCCGCACGAGGTGGAAGGCGTCGCCTCGGCGCTCCGCCGTGCGCTGGAGATGCCGCTCGCGGAACG
CCGGGATCGCCACGCGCGGATGATGACCGTGCTCAACACCAACACCATCGATCATTGGGCGCAGAACTTCCTTGATGCCC
TCACCGACCCGCCGGCGCTCGACGCTTTATTGAGCGCGCGATCCAATGGGTTACGACCCTCGGCCCCTGCCTGCCTGATG
GACTGGCAGGGGCTGCATCGCGCTCAGGATCTGTAG

Upstream 100 bases:

>100_bases
CGGCCGCAACCTTCCCGTCGCCGATCTGGTGCTGCCCGAAGCGCAGGACGTGCGCGCCCTGCTCGCCACCCTTGTCGCCC
AACAGCCGGAGGAGTGACCC

Downstream 100 bases:

>100_bases
CGCACCCCTCTCGTACTCGACGTGAAGTGGCGGAGCGCAGACGCCGTCGTGCGGACGGCGCCGCTCCGCATCCGTATTTC
TTGTGACGGTGCAGGCTCGG

Product: OtsA trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSRLIVVSNRVAVPEAGKVSAGGLAVAVNAALKERSGIWFGWSGKITEQPSDQPAISMRNGVKYAVVDLKEDDYQEYYNG
FANRVLWPILHYRVDLAEFVRSDLSGYIRVNKNFAEHVSKIIEEDDIIWVHDYHMMPLARYLRERGHKNRIGFFLHIPLP
PPDLIQALPRHGEIVGSLVHYDLVGFQTESDRDNFGRYLEGRGARPGNDGTSYEIAGRRVRIGAFPVAIETESFARRARH
AMRTNFMKDFRASLTGKKMVLGVDRLDYSKGIPNRLEAFETLLRTAPEWHERVTFVQITPKSRTSVPEYAEMDRYVSTKA
GQINGEFGDVSWTPLRYVNKTYSRSALAGLYRAADVAMVTPLRDGMNLVAKEFVAAQDPEDPGVLLLSQFAGAAAEMGGG
ALMVNPHEVEGVASALRRALEMPLAERRDRHARMMTVLNTNTIDHWAQNFLDALTDPPALDALLSARSNGLRPSAPACLM
DWQGLHRAQDL

Sequences:

>Translated_491_residues
MSRLIVVSNRVAVPEAGKVSAGGLAVAVNAALKERSGIWFGWSGKITEQPSDQPAISMRNGVKYAVVDLKEDDYQEYYNG
FANRVLWPILHYRVDLAEFVRSDLSGYIRVNKNFAEHVSKIIEEDDIIWVHDYHMMPLARYLRERGHKNRIGFFLHIPLP
PPDLIQALPRHGEIVGSLVHYDLVGFQTESDRDNFGRYLEGRGARPGNDGTSYEIAGRRVRIGAFPVAIETESFARRARH
AMRTNFMKDFRASLTGKKMVLGVDRLDYSKGIPNRLEAFETLLRTAPEWHERVTFVQITPKSRTSVPEYAEMDRYVSTKA
GQINGEFGDVSWTPLRYVNKTYSRSALAGLYRAADVAMVTPLRDGMNLVAKEFVAAQDPEDPGVLLLSQFAGAAAEMGGG
ALMVNPHEVEGVASALRRALEMPLAERRDRHARMMTVLNTNTIDHWAQNFLDALTDPPALDALLSARSNGLRPSAPACLM
DWQGLHRAQDL
>Mature_490_residues
SRLIVVSNRVAVPEAGKVSAGGLAVAVNAALKERSGIWFGWSGKITEQPSDQPAISMRNGVKYAVVDLKEDDYQEYYNGF
ANRVLWPILHYRVDLAEFVRSDLSGYIRVNKNFAEHVSKIIEEDDIIWVHDYHMMPLARYLRERGHKNRIGFFLHIPLPP
PDLIQALPRHGEIVGSLVHYDLVGFQTESDRDNFGRYLEGRGARPGNDGTSYEIAGRRVRIGAFPVAIETESFARRARHA
MRTNFMKDFRASLTGKKMVLGVDRLDYSKGIPNRLEAFETLLRTAPEWHERVTFVQITPKSRTSVPEYAEMDRYVSTKAG
QINGEFGDVSWTPLRYVNKTYSRSALAGLYRAADVAMVTPLRDGMNLVAKEFVAAQDPEDPGVLLLSQFAGAAAEMGGGA
LMVNPHEVEGVASALRRALEMPLAERRDRHARMMTVLNTNTIDHWAQNFLDALTDPPALDALLSARSNGLRPSAPACLMD
WQGLHRAQDL

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=455, Percent_Identity=46.8131868131868, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI115533558, Length=420, Percent_Identity=29.0476190476191, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI115533560, Length=420, Percent_Identity=29.0476190476191, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71987012, Length=428, Percent_Identity=30.1401869158879, Blast_Score=171, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=33.1914893617021, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6323537, Length=449, Percent_Identity=28.0623608017817, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6320279, Length=401, Percent_Identity=26.9326683291771, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323917, Length=448, Percent_Identity=26.1160714285714, Blast_Score=179, Evalue=8e-46,
Organism=Drosophila melanogaster, GI19920676, Length=487, Percent_Identity=38.8090349075975, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 54962; Mature: 54831

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIVVSNRVAVPEAGKVSAGGLAVAVNAALKERSGIWFGWSGKITEQPSDQPAISMRN
CCCEEEEECCEECCCCCCCCCCCEEEEEHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHC
GVKYAVVDLKEDDYQEYYNGFANRVLWPILHYRVDLAEFVRSDLSGYIRVNKNFAEHVSK
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
IIEEDDIIWVHDYHMMPLARYLRERGHKNRIGFFLHIPLPPPDLIQALPRHGEIVGSLVH
HHCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHH
YDLVGFQTESDRDNFGRYLEGRGARPGNDGTSYEIAGRRVRIGAFPVAIETESFARRARH
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEEEEEEEECHHHHHHHHH
AMRTNFMKDFRASLTGKKMVLGVDRLDYSKGIPNRLEAFETLLRTAPEWHERVTFVQITP
HHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHCCCCHHHCEEEEEECC
KSRTSVPEYAEMDRYVSTKAGQINGEFGDVSWTPLRYVNKTYSRSALAGLYRAADVAMVT
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
PLRDGMNLVAKEFVAAQDPEDPGVLLLSQFAGAAAEMGGGALMVNPHEVEGVASALRRAL
CCHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHCCCCEEEECHHHHHHHHHHHHHHH
EMPLAERRDRHARMMTVLNTNTIDHWAQNFLDALTDPPALDALLSARSNGLRPSAPACLM
HCCHHHHHHHHHHEEHEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHH
DWQGLHRAQDL
HHHHHHHHCCC
>Mature Secondary Structure 
SRLIVVSNRVAVPEAGKVSAGGLAVAVNAALKERSGIWFGWSGKITEQPSDQPAISMRN
CCEEEEECCEECCCCCCCCCCCEEEEEHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHC
GVKYAVVDLKEDDYQEYYNGFANRVLWPILHYRVDLAEFVRSDLSGYIRVNKNFAEHVSK
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
IIEEDDIIWVHDYHMMPLARYLRERGHKNRIGFFLHIPLPPPDLIQALPRHGEIVGSLVH
HHCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHH
YDLVGFQTESDRDNFGRYLEGRGARPGNDGTSYEIAGRRVRIGAFPVAIETESFARRARH
HHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEEEEEEEECHHHHHHHHH
AMRTNFMKDFRASLTGKKMVLGVDRLDYSKGIPNRLEAFETLLRTAPEWHERVTFVQITP
HHHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCHHHHHHHHHHHCCCCHHHCEEEEEECC
KSRTSVPEYAEMDRYVSTKAGQINGEFGDVSWTPLRYVNKTYSRSALAGLYRAADVAMVT
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
PLRDGMNLVAKEFVAAQDPEDPGVLLLSQFAGAAAEMGGGALMVNPHEVEGVASALRRAL
CCHHHHHHHHHHHHHCCCCCCCCEEEHHHHCCHHHHCCCCEEEECHHHHHHHHHHHHHHH
EMPLAERRDRHARMMTVLNTNTIDHWAQNFLDALTDPPALDALLSARSNGLRPSAPACLM
HCCHHHHHHHHHHEEHEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHH
DWQGLHRAQDL
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]