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The map label for this gene is iorA [H]

Identifier: 158422044

GI number: 158422044

Start: 476097

End: 476558

Strand: Reverse

Name: iorA [H]

Synonym: AZC_0420

Alternate gene names: 158422044

Gene position: 476558-476097 (Counterclockwise)

Preceding gene: 158422045

Following gene: 158422043

Centisome position: 8.87

GC content: 69.91

Gene sequence:

>462_bases
ATGCCCATCACCCTTTCCATCAACGGCCAGACCCGGACGGTCGAGGCCGAGCCGGACACGCCCCTTCTCTGGGTGCTGCG
CGACGAACTGGACCTCACCGGCACCAAGTTCGGCTGCGGCCAGGCCCTCTGCGGCGCCTGCACGGTGCATGTGGACGGCG
CCGCCGTTCGCTCCTGCCAGACCGCCATCGGCGACGTTGGCGACGCCAAGGTCACCACCATCGAGGGCCTGAAGAGCCGC
GAGGCCGAGGCCGTCCAGGCGGCCTGGCGTCAGCTCGATGTGGTCCAGTGCGGCTATTGCCAGTCCGGCCAGATCATGTC
CGCCGTGGCGCTGCTCACCGACAACAAGAAGCCCACGGACGCCGACATCGACGCCGCCATGGACGGCAATGTCTGCCGCT
GTGCCACCTACCAGCGCGTCCGCGCCGCCATCCACGACGCCGCCCGCCGGCTGGAGGCCTGA

Upstream 100 bases:

>100_bases
GCAGCATTTCGAAATCACACTCTGGAACGCCTCCAGGTTGTGATTACGTAAGGCTCGTTTTTCGAACCGCCGCGCCTCAG
CCGGCGGCAGGGAGCCCGCC

Downstream 100 bases:

>100_bases
GCCATGAATGCCATTCTCAAAGCCGCCGAAGCCGGTCTCTCGCGCCGCGCCATCCTCAGGGCCATGGGCGGGGTCGGCAT
CGGCCTCGTCATCGGCACCG

Product: isoquinoline 1-oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPITLSINGQTRTVEAEPDTPLLWVLRDELDLTGTKFGCGQALCGACTVHVDGAAVRSCQTAIGDVGDAKVTTIEGLKSR
EAEAVQAAWRQLDVVQCGYCQSGQIMSAVALLTDNKKPTDADIDAAMDGNVCRCATYQRVRAAIHDAARRLEA

Sequences:

>Translated_153_residues
MPITLSINGQTRTVEAEPDTPLLWVLRDELDLTGTKFGCGQALCGACTVHVDGAAVRSCQTAIGDVGDAKVTTIEGLKSR
EAEAVQAAWRQLDVVQCGYCQSGQIMSAVALLTDNKKPTDADIDAAMDGNVCRCATYQRVRAAIHDAARRLEA
>Mature_152_residues
PITLSINGQTRTVEAEPDTPLLWVLRDELDLTGTKFGCGQALCGACTVHVDGAAVRSCQTAIGDVGDAKVTTIEGLKSRE
AEAVQAAWRQLDVVQCGYCQSGQIMSAVALLTDNKKPTDADIDAAMDGNVCRCATYQRVRAAIHDAARRLEA

Specific function: Specific towards N-containing N-heterocyclic substrates, including isoquinoline, isoquinolin-5-ol, phthalazine and quinazoline [H]

COG id: COG2080

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, small subunit CoxS/CutS homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 2Fe-2S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI91823271, Length=157, Percent_Identity=35.6687898089172, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786480, Length=169, Percent_Identity=41.4201183431953, Blast_Score=118, Evalue=1e-28,
Organism=Escherichia coli, GI1789232, Length=150, Percent_Identity=37.3333333333333, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17540638, Length=158, Percent_Identity=34.1772151898734, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17539860, Length=155, Percent_Identity=34.1935483870968, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32566215, Length=161, Percent_Identity=34.7826086956522, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17737937, Length=147, Percent_Identity=36.734693877551, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647197, Length=164, Percent_Identity=32.3170731707317, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24647195, Length=164, Percent_Identity=32.3170731707317, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24647199, Length=158, Percent_Identity=32.9113924050633, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24647193, Length=160, Percent_Identity=32.5, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24647201, Length=158, Percent_Identity=30.379746835443, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002888
- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR001041 [H]

Pfam domain/function: PF00111 Fer2; PF01799 Fer2_2 [H]

EC number: =1.3.99.16 [H]

Molecular weight: Translated: 16281; Mature: 16149

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
5.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITLSINGQTRTVEAEPDTPLLWVLRDELDLTGTKFGCGQALCGACTVHVDGAAVRSCQ
CEEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHCEEEEEECCHHHHHHH
TAIGDVGDAKVTTIEGLKSREAEAVQAAWRQLDVVQCGYCQSGQIMSAVALLTDNKKPTD
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHEEHCCCCCCCC
ADIDAAMDGNVCRCATYQRVRAAIHDAARRLEA
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PITLSINGQTRTVEAEPDTPLLWVLRDELDLTGTKFGCGQALCGACTVHVDGAAVRSCQ
EEEEEECCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHCEEEEEECCHHHHHHH
TAIGDVGDAKVTTIEGLKSREAEAVQAAWRQLDVVQCGYCQSGQIMSAVALLTDNKKPTD
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHEEHCCCCCCCC
ADIDAAMDGNVCRCATYQRVRAAIHDAARRLEA
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7782304; 8157655 [H]