Click here to switch to the map view.

The map label for this gene is phaA [H]

Identifier: 158421930

GI number: 158421930

Start: 355974

End: 357152

Strand: Reverse

Name: phaA [H]

Synonym: AZC_0306

Alternate gene names: 158421930

Gene position: 357152-355974 (Counterclockwise)

Preceding gene: 158421932

Following gene: 158421929

Centisome position: 6.65

GC content: 68.62

Gene sequence:

>1179_bases
ATGACCGACGACGTCGTCATCGTCAGCGCCGCCCGCACCCCCGTGGGGTCCTTCAACGGCGCCCTGTCCACGCTTCCGGC
GCACCAGCTGGGCGCCATCGCCATCAAGGCCGCTCTGGAGCGGGCCGGCGTTGCTGCATCCGAAGTCAGCGAAGTGATTC
TCGGCCAGGTGCTGACCGCCGCCCAGGGCCAGAATCCCGCCCGCCAGGCCTCCATCGCCGCCGGCGTTCCGATCGAGAGC
CCGGCCTGGCAGGTCAACCAGGTGTGCGGCTCGGGGCTGCGTTCCGTTGCGCTTGGATATCAGGCCATCAAGAATGGCGA
CAGCTCGCTGGTGGTGGCCGGCGGCCAGGAATCCATGAGCCAGTCCACCCATGCCGCGCACCTGCGAAACGGCACCCGCA
TGGGCTCGCTCGACCTTGTTGACACCATGATCAAGGACGGCCTGTGGGACGCCTTCAACGGCTACCACATGGGCAACACC
GCCGAGAACGTGGCGACCCAGTTCCAGATCACCCGCGCCGAGCAGGACGAGTTCGCCGTCGCTTCCCAGCACAAGGCCGA
GGCGGCCCAGAAGGCCGGGCGCTTCAAGGAGGAAATCGTCCCCGTCACCATCTCCAGCCGCAAGGGCGACGTGGTGGTGG
ATACGGACGAATATCCCCGCCACGGCGCCACCATCGAGGCGATGCAGAAGCTGAAGCCGGCCTTCGTCAAGGACGGCACG
GTGACCGCCGCGAACGCCTCGGGCATCAATGACGGCGGCGCCGCCGTGGTGCTCATGAGCGCCGAGCGCGCCGCCAAGGA
AGGCAAGACCCCGCTCGCCCGCATCGTCTCCTGGGCGCAGGCCGGCGTCGATCCGGCCGTGATGGGCACCGGCCCGATCC
CCGCCTCCAAGCTGGCTCTGGAGAAGGCCGGCTGGACCGTCGATGATCTCGACCTCATCGAGGCCAACGAGGCGTTCGCC
GCCCAGGCCATCGCCGTCAACAAGGGCCTCGGCTGGGATACCGCGAAGGTCAATGTGAACGGCGGCGCCATCGCCATCGG
CCATCCCATCGGCGCCTCCGGCACCCGCATCCTCGTGACCCTGCTGCACGAGATGCAGAAGCGCGACGCCAAGAAGGGCC
TCGCCACGCTCTGCATCGGCGGCGGCATGGGCATCGCGCTCTGCGTCGAGCGCCCCTGA

Upstream 100 bases:

>100_bases
TGAACCACTGATCTGGATCATTGACAGCGGGTTCGCGGGGCAACCACGATGCGACCGCATCGAGTGCGGCGCACGCAAGG
CCGCCCCAGGAGAGAAGCCC

Downstream 100 bases:

>100_bases
ACCGGGGCCGGGACCCGCGCGGCCGCCGGGGGGCGGCCGCGGTTTTCCAGATGACGTTACCCGTTTGCGAGAAGCCCGAA
GATTGCCCGGCCTGTGCCGG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTDDVVIVSAARTPVGSFNGALSTLPAHQLGAIAIKAALERAGVAASEVSEVILGQVLTAAQGQNPARQASIAAGVPIES
PAWQVNQVCGSGLRSVALGYQAIKNGDSSLVVAGGQESMSQSTHAAHLRNGTRMGSLDLVDTMIKDGLWDAFNGYHMGNT
AENVATQFQITRAEQDEFAVASQHKAEAAQKAGRFKEEIVPVTISSRKGDVVVDTDEYPRHGATIEAMQKLKPAFVKDGT
VTAANASGINDGGAAVVLMSAERAAKEGKTPLARIVSWAQAGVDPAVMGTGPIPASKLALEKAGWTVDDLDLIEANEAFA
AQAIAVNKGLGWDTAKVNVNGGAIAIGHPIGASGTRILVTLLHEMQKRDAKKGLATLCIGGGMGIALCVERP

Sequences:

>Translated_392_residues
MTDDVVIVSAARTPVGSFNGALSTLPAHQLGAIAIKAALERAGVAASEVSEVILGQVLTAAQGQNPARQASIAAGVPIES
PAWQVNQVCGSGLRSVALGYQAIKNGDSSLVVAGGQESMSQSTHAAHLRNGTRMGSLDLVDTMIKDGLWDAFNGYHMGNT
AENVATQFQITRAEQDEFAVASQHKAEAAQKAGRFKEEIVPVTISSRKGDVVVDTDEYPRHGATIEAMQKLKPAFVKDGT
VTAANASGINDGGAAVVLMSAERAAKEGKTPLARIVSWAQAGVDPAVMGTGPIPASKLALEKAGWTVDDLDLIEANEAFA
AQAIAVNKGLGWDTAKVNVNGGAIAIGHPIGASGTRILVTLLHEMQKRDAKKGLATLCIGGGMGIALCVERP
>Mature_391_residues
TDDVVIVSAARTPVGSFNGALSTLPAHQLGAIAIKAALERAGVAASEVSEVILGQVLTAAQGQNPARQASIAAGVPIESP
AWQVNQVCGSGLRSVALGYQAIKNGDSSLVVAGGQESMSQSTHAAHLRNGTRMGSLDLVDTMIKDGLWDAFNGYHMGNTA
ENVATQFQITRAEQDEFAVASQHKAEAAQKAGRFKEEIVPVTISSRKGDVVVDTDEYPRHGATIEAMQKLKPAFVKDGTV
TAANASGINDGGAAVVLMSAERAAKEGKTPLARIVSWAQAGVDPAVMGTGPIPASKLALEKAGWTVDDLDLIEANEAFAA
QAIAVNKGLGWDTAKVNVNGGAIAIGHPIGASGTRILVTLLHEMQKRDAKKGLATLCIGGGMGIALCVERP

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=59.9489795918367, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=43.6224489795918, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI4557237, Length=391, Percent_Identity=45.0127877237852, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI4501853, Length=397, Percent_Identity=39.2947103274559, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=32.1513002364066, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI194353979, Length=392, Percent_Identity=30.1020408163265, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=56.8123393316195, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1788554, Length=390, Percent_Identity=57.4358974358974, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1787663, Length=398, Percent_Identity=42.2110552763819, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=39.8514851485149, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788683, Length=412, Percent_Identity=32.7669902912621, Blast_Score=194, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=45.5012853470437, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=41.2987012987013, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17535921, Length=395, Percent_Identity=40.253164556962, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=30.188679245283, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=390, Percent_Identity=32.0512820512821, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=43.7185929648241, Blast_Score=293, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=36.6666666666667, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=59.020618556701, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=47.1938775510204, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=43.8775510204082, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=31.924882629108, Blast_Score=188, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40426; Mature: 40295

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDVVIVSAARTPVGSFNGALSTLPAHQLGAIAIKAALERAGVAASEVSEVILGQVLTA
CCCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AQGQNPARQASIAAGVPIESPAWQVNQVCGSGLRSVALGYQAIKNGDSSLVVAGGQESMS
HCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHH
QSTHAAHLRNGTRMGSLDLVDTMIKDGLWDAFNGYHMGNTAENVATQFQITRAEQDEFAV
HHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHEEEEEECCCCHHHH
ASQHKAEAAQKAGRFKEEIVPVTISSRKGDVVVDTDEYPRHGATIEAMQKLKPAFVKDGT
HHHHHHHHHHHHCCHHHHEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHCCCHHCCCCE
VTAANASGINDGGAAVVLMSAERAAKEGKTPLARIVSWAQAGVDPAVMGTGPIPASKLAL
EEECCCCCCCCCCCEEEEEECHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
EKAGWTVDDLDLIEANEAFAAQAIAVNKGLGWDTAKVNVNGGAIAIGHPIGASGTRILVT
HHCCCCCCCCHHEECCHHHHHHEEEEECCCCCCEEEEEECCCEEEECCCCCCCCHHHHHH
LLHEMQKRDAKKGLATLCIGGGMGIALCVERP
HHHHHHHHHHHCCCEEEEEECCCEEEEEEECC
>Mature Secondary Structure 
TDDVVIVSAARTPVGSFNGALSTLPAHQLGAIAIKAALERAGVAASEVSEVILGQVLTA
CCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AQGQNPARQASIAAGVPIESPAWQVNQVCGSGLRSVALGYQAIKNGDSSLVVAGGQESMS
HCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHH
QSTHAAHLRNGTRMGSLDLVDTMIKDGLWDAFNGYHMGNTAENVATQFQITRAEQDEFAV
HHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHEEEEEECCCCHHHH
ASQHKAEAAQKAGRFKEEIVPVTISSRKGDVVVDTDEYPRHGATIEAMQKLKPAFVKDGT
HHHHHHHHHHHHCCHHHHEEEEEEECCCCCEEECCCCCCCCCCHHHHHHHCCCHHCCCCE
VTAANASGINDGGAAVVLMSAERAAKEGKTPLARIVSWAQAGVDPAVMGTGPIPASKLAL
EEECCCCCCCCCCCEEEEEECHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHH
EKAGWTVDDLDLIEANEAFAAQAIAVNKGLGWDTAKVNVNGGAIAIGHPIGASGTRILVT
HHCCCCCCCCHHEECCHHHHHHEEEEECCCCCCEEEEEECCCEEEECCCCCCCCHHHHHH
LLHEMQKRDAKKGLATLCIGGGMGIALCVERP
HHHHHHHHHHHCCCEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566717 [H]