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The map label for this gene is rstB [H]

Identifier: 158421789

GI number: 158421789

Start: 194784

End: 196136

Strand: Reverse

Name: rstB [H]

Synonym: AZC_0165

Alternate gene names: 158421789

Gene position: 196136-194784 (Counterclockwise)

Preceding gene: 158421790

Following gene: 158421786

Centisome position: 3.65

GC content: 72.43

Gene sequence:

>1353_bases
TTGACGCGCTTCAAGCTGCCGCTGCGGCTCGCGCTCATCATCGCGGGCGGCCTCGTCATCGCCCAGATGCTCATGACGGC
GGCCTATTTCACGGATCGGCGTCTGAACGGCGAAGGTGTCGCCGGCTCCATGGCGACGCAGGTGGGAGCCCTCGCGCAAC
TGCTGGACCGGCTGCCGGAGCCGGACCGCGCGCTTGCGCTCGATGCCGTCAGCGGCCCCGGCTTTCGCGCCCGCATCGCG
CCGCAGCCCGAGACAGGAGAGACGAAGCGCCCGCCGCTGCTGGAGCGTGTGCCCCTGCTCGAGCGCCTCATCCGGGCGCA
ACTGGGGAAGAACGATCCGCGCGAGGTGACCGTCGTCCTCGACACGGCCTTCGGCGGGCGCTGGATTCCGATGATGCTGT
CCTGGCCCGCACGGGAATTGCGCATGACCGTCGGGCTGGCCGATGGCCGCTTCCTGCTGGTGCGCGCCCGGGGGGAACTG
ACCGCGCGGCTTTTCGGCGTTCCCGTCGGCCTCGTGGCCGGGCTGCTGGGCATCGCGGTGGCGCTCGCCACAGTCCTCGC
GGTGCGGCGGGAAACCCGGCCGATTTCCGAGCTTTCGGCCGCGCTGGACCGCTTCGGCACCAGCCTTGAGCCCCAGCGTG
TGGAGCCCCGCGGCGCGCCGGACGTGCGGCGGCTGGCTGAGACCTTCGACGCCATGCAGCGGCGCATCGTCGAACTGGTG
CGCAACCGCAGCCTCGTGCTCGGGGCCATGAGCCATGACCTCGCCACCTATGTCACGCGGCTGCGCCTGCGCATCGCGCT
CCTGCCGGAGGGTCCGCAGACGGCCCGCGCCCAGCGCGACCTCGAGGAGATGCAGGCGCTGCTCGCCGACACGCTCGATT
TCGCGCGCGGCAGCTTCACGGGGCGCGACGGCGCGAGCACCGACTTTGCGGCGCTGGTGGCGGAGGAATGCGCGGGGCGA
GAGGCGGAAGGGGCCCCTGTCCATTTCAGCGCGCCGTCCGTTCCCTGCCGCGTCGCGGCGGCGCCCTCGGCTCTGAAGCG
TCTGGCGGCGAACCTCATCGGCAATGCCATCGCCTATGGCGGGCGGGCGGACGTCTCCCTCATGAGGGACGGGGATGCGG
TGGATCTGGTGGTGGAGGATCGTGGGCCCGGCGTCCCCGAGGCCGAGCGTGAGCGGGTGTTCGAGCCCTTCTACCGGCTC
GATGCCTCCCGCAGCCGCGAGACCGGCGGCGCGGGGCTGGGGCTCACCATCGTGCGGCAGATCACCGACAGCATCGGCGG
CACCGTCGGGATCGCGGACCGTCCCGGCGGCGGCGCGCGCGTGACGGCGCGCCTGCCTCTGGTGGCGGATTGA

Upstream 100 bases:

>100_bases
GCCGCCGCCTGTCCTTGGTCGCGCCGGAGGCCGCACAACTCATCAAGACCGTACGCAACGGCGGCTACATCTTCGCCCTT
CCTCCCGGAGGCAAAGGCAA

Downstream 100 bases:

>100_bases
CCCCGCGGGGGATCACTGGCGCATGCGCGCCAGCACGTCGAGGTTCGGGTAGCCGTCCGGGATCATGCCCGCGCGGGTCT
GGAAGTCCTGCACGGCCACG

Product: histidine kinase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTRFKLPLRLALIIAGGLVIAQMLMTAAYFTDRRLNGEGVAGSMATQVGALAQLLDRLPEPDRALALDAVSGPGFRARIA
PQPETGETKRPPLLERVPLLERLIRAQLGKNDPREVTVVLDTAFGGRWIPMMLSWPARELRMTVGLADGRFLLVRARGEL
TARLFGVPVGLVAGLLGIAVALATVLAVRRETRPISELSAALDRFGTSLEPQRVEPRGAPDVRRLAETFDAMQRRIVELV
RNRSLVLGAMSHDLATYVTRLRLRIALLPEGPQTARAQRDLEEMQALLADTLDFARGSFTGRDGASTDFAALVAEECAGR
EAEGAPVHFSAPSVPCRVAAAPSALKRLAANLIGNAIAYGGRADVSLMRDGDAVDLVVEDRGPGVPEAERERVFEPFYRL
DASRSRETGGAGLGLTIVRQITDSIGGTVGIADRPGGGARVTARLPLVAD

Sequences:

>Translated_450_residues
MTRFKLPLRLALIIAGGLVIAQMLMTAAYFTDRRLNGEGVAGSMATQVGALAQLLDRLPEPDRALALDAVSGPGFRARIA
PQPETGETKRPPLLERVPLLERLIRAQLGKNDPREVTVVLDTAFGGRWIPMMLSWPARELRMTVGLADGRFLLVRARGEL
TARLFGVPVGLVAGLLGIAVALATVLAVRRETRPISELSAALDRFGTSLEPQRVEPRGAPDVRRLAETFDAMQRRIVELV
RNRSLVLGAMSHDLATYVTRLRLRIALLPEGPQTARAQRDLEEMQALLADTLDFARGSFTGRDGASTDFAALVAEECAGR
EAEGAPVHFSAPSVPCRVAAAPSALKRLAANLIGNAIAYGGRADVSLMRDGDAVDLVVEDRGPGVPEAERERVFEPFYRL
DASRSRETGGAGLGLTIVRQITDSIGGTVGIADRPGGGARVTARLPLVAD
>Mature_449_residues
TRFKLPLRLALIIAGGLVIAQMLMTAAYFTDRRLNGEGVAGSMATQVGALAQLLDRLPEPDRALALDAVSGPGFRARIAP
QPETGETKRPPLLERVPLLERLIRAQLGKNDPREVTVVLDTAFGGRWIPMMLSWPARELRMTVGLADGRFLLVRARGELT
ARLFGVPVGLVAGLLGIAVALATVLAVRRETRPISELSAALDRFGTSLEPQRVEPRGAPDVRRLAETFDAMQRRIVELVR
NRSLVLGAMSHDLATYVTRLRLRIALLPEGPQTARAQRDLEEMQALLADTLDFARGSFTGRDGASTDFAALVAEECAGRE
AEGAPVHFSAPSVPCRVAAAPSALKRLAANLIGNAIAYGGRADVSLMRDGDAVDLVVEDRGPGVPEAERERVFEPFYRLD
ASRSRETGGAGLGLTIVRQITDSIGGTVGIADRPGGGARVTARLPLVAD

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=295, Percent_Identity=29.1525423728814, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789808, Length=267, Percent_Identity=30.3370786516854, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=32.034632034632, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789149, Length=306, Percent_Identity=26.4705882352941, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=25.5737704918033, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=255, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786912, Length=272, Percent_Identity=28.6764705882353, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=24.7967479674797, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081816, Length=365, Percent_Identity=25.2054794520548, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=114, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=220, Percent_Identity=22.7272727272727, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=288, Percent_Identity=28.125, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790551, Length=295, Percent_Identity=25.7627118644068, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=299, Percent_Identity=24.0802675585284, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=74, Percent_Identity=41.8918918918919, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48218; Mature: 48087

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFKLPLRLALIIAGGLVIAQMLMTAAYFTDRRLNGEGVAGSMATQVGALAQLLDRLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PDRALALDAVSGPGFRARIAPQPETGETKRPPLLERVPLLERLIRAQLGKNDPREVTVVL
CCCEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
DTAFGGRWIPMMLSWPARELRMTVGLADGRFLLVRARGELTARLFGVPVGLVAGLLGIAV
EECCCCEEEHHHCCCCHHHHEEEEEECCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHH
ALATVLAVRRETRPISELSAALDRFGTSLEPQRVEPRGAPDVRRLAETFDAMQRRIVELV
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RNRSLVLGAMSHDLATYVTRLRLRIALLPEGPQTARAQRDLEEMQALLADTLDFARGSFT
HCCCEEEEHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GRDGASTDFAALVAEECAGREAEGAPVHFSAPSVPCRVAAAPSALKRLAANLIGNAIAYG
CCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCC
GRADVSLMRDGDAVDLVVEDRGPGVPEAERERVFEPFYRLDASRSRETGGAGLGLTIVRQ
CCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
ITDSIGGTVGIADRPGGGARVTARLPLVAD
HHHHHCCEEEECCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TRFKLPLRLALIIAGGLVIAQMLMTAAYFTDRRLNGEGVAGSMATQVGALAQLLDRLPE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PDRALALDAVSGPGFRARIAPQPETGETKRPPLLERVPLLERLIRAQLGKNDPREVTVVL
CCCEEEEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEE
DTAFGGRWIPMMLSWPARELRMTVGLADGRFLLVRARGELTARLFGVPVGLVAGLLGIAV
EECCCCEEEHHHCCCCHHHHEEEEEECCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHH
ALATVLAVRRETRPISELSAALDRFGTSLEPQRVEPRGAPDVRRLAETFDAMQRRIVELV
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RNRSLVLGAMSHDLATYVTRLRLRIALLPEGPQTARAQRDLEEMQALLADTLDFARGSFT
HCCCEEEEHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GRDGASTDFAALVAEECAGREAEGAPVHFSAPSVPCRVAAAPSALKRLAANLIGNAIAYG
CCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCC
GRADVSLMRDGDAVDLVVEDRGPGVPEAERERVFEPFYRLDASRSRETGGAGLGLTIVRQ
CCCCEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
ITDSIGGTVGIADRPGGGARVTARLPLVAD
HHHHHCCEEEECCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]