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The map label for this gene is ligA

Identifier: 158421773

GI number: 158421773

Start: 178214

End: 180433

Strand: Reverse

Name: ligA

Synonym: AZC_0149

Alternate gene names: 158421773

Gene position: 180433-178214 (Counterclockwise)

Preceding gene: 158421775

Following gene: 158421772

Centisome position: 3.36

GC content: 69.32

Gene sequence:

>2220_bases
ATGACCGCGAACCGCCCCGCCCTTCCGACCCGCGACAAGGCCGTCTCCGACCTCTCGGCGACCGAGGCCTCCGACGAACA
TGCCGCACTCTCGCAGGAGATCGCGTCCCACGACGCGCGCTACTATCAGGAGGACGCGCCGGTCATTTCGGATGCCGATT
ACGACCGGCTGCGGCGGCGCTATGAAGACATCGAGGCGCGCTTCCCCGCGCTCAAGAATGACGACAGCCTCTCGCGCAAG
GTGGGCGCGGCGCCTTCGGCGAAATTCGCCAAGGTGGTCCATCAGGTGCCCATGCTCTCGCTCCAGAACGCCTTCTCCGA
CGAGGAGGTCGAGGAGTTCGTGGCCCGCGTGCGGCGCTTCCTCAATCTGGCCGAGAGCGCGGAACTCGCCTTCACCGCCG
AGCCGAAGATCGACGGCCTGTCCTGCTCCCTGCGCTACGAGGGCGGCGTTCTGGTGCGGGCGGCGACGCGCGGCGACGGG
ACGCAGGGCGAGGACGTGACCGCCAACGTGCGCACCATTGCCGAAATCCCCCATCGCCTGACGGGCGCGGGCGTGCCGGA
CATCATCGATATCCGGGGCGAGGTCTATATGGGCAAGGCGGACTTCCTCGCCCTCAACGCCCGGCAGGAAGAGGCCGGCA
AGCCGGTGTTCGCCAATCCGCGCAATGCGGCGGCGGGCTCGCTCCGCCAGCTCGATCCCGAAATCACCCGGTCGCGCCCC
TTGCGCTTCTTCGCTTATGCCTGGGGGCAGGCCGAGCCCGGCCCGCCCGGCGCCAGCCAGCATGAGGTGGTGGACGCCTT
CCGGCGCTTCGGCCTGCCCACCAATCCGCTCACCATCCGCGTCACCGATGCGGCGGGGCTGCTGGCCCATTACCGGAAGA
TCGGGGCGGAGCGCGCGAGCCTCGGCTATGACATCGACGGCGTGGTCTACAAGGTGGACAGCCTCGCGCTTCAGGAGCGC
CTCGGCTTCGTCTCCCGCTCGCCGCGCTGGGCCATCGCCCACAAGTTCGCCGCCGAGCAGGCGACCACGGTCATCGAGGC
CATCGAGATCCAGGTCGGCCGCACCGGGGCGCTCACGCCGGTGGCGAAGCTCACGCCCGTCACCGTGGGCGGCGTCGTCG
TCTCCAACGCGACGCTGCACAATGAGGATTACATCCGCGCCATCGGCGGCAATGGCGAAGAGATCCGCGAGGGCATCGAC
ATCCGCGTCGGCGACACCGTGGTGGTCCAGCGCGCCGGCGATGTGATCCCGCAGGTGGTCTCCGTGGTGCTGGACAAGCG
CCCGGCCGGGGCCACGGCCTATCAGTTCCCGCACCACTGCCCCGTCTGCAACAGCCAGGCGGTGCGCGAGGTGGACCCCA
AGACCGGCAAGCAGGATGCGGTGCGCCGCTGCACCGGCGGCCTCATCTGCCCGGCGCAGGCGGTGGAGCGGCTGCGCCAC
TTCGTCTCCCGCAACGCCTTCGACATCGAGGGGCTCGGCGAGAAGCAGGCCCACGCTTTCTATGAGTGGAAGCTGATCGC
GGAGCCGGCGGACATCTTCCGCCTTCAGGCCCGCAACGAACAGTCGCTCTCGAAGCTGGAAAACCGCGAAGGCTGGGGCA
GGACCTCGGCGCAGAACCTCTTCGCCGCCATCGAGGCCCGGCGCACCATCGCGCTCGACCGCTTCATCTTCGCCCTCGGC
ATCCGCCATGTGGGCGAGACCAATGCCAAGCGGCTCGCCCGCCATTACGGCTCGGTGGAGGCCCTGCGCGATGCCGCCAT
CGGCGCCGAGATGCCCACCGAGATGGAGGAGGCCTCCGAGGAGACCCCGCCCACCCGCCGCCGCAAGCCGCAGGGCAATG
AGGTGTGGCAGGAGATGACGGACATCGACGGCATCGGCGCCGTGGTGGCCGAGGCGGTGGTGGAGTTCTTCCGCGAGCCG
CACAACCGGCAGGTCGTGGAAGAGCTGCTGAAGGAAGTGACGCCCCAGCCGCTGGAGGCCGTCGCCTCAACGTCGCCCGT
CTCGGGCAAGACCGTGGTCTTCACCGGATCCTTGGAGCGCATGACCCGCGACGAGGCCAAAGCCATGGCCGAGCGCCTCG
GCGCCAAGGTCGCCGGCTCGGTCTCCGGCAAGACCGACCTTCTGGTCGCTGGCCCCGGCGCGGGCTCCAAGCTGGAGAAG
GCCCGCAGCCTCGGGGTGAAGACCATCACCGAGGACGAGTGGTTCGATCTGGTGGGCTGA

Upstream 100 bases:

>100_bases
TTTGCCTTGGGAGGTACATTGGACGGGTTACAAAAAAGAGACAATCGCCAGTTGCAGGCGCCCATTGCTGAACCGGGCGG
CTTCCAGCAAAAAGACGTCC

Downstream 100 bases:

>100_bases
GCCTGCCGCAGCTGACGTAAAGGTGGCGGCGCGGCATCGGCTTGGCTAAGGTGTCATCGGGAAGGTGCGGCCCTTTGGGG
CCGTGAAAAGGGAATCCGGT

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MTANRPALPTRDKAVSDLSATEASDEHAALSQEIASHDARYYQEDAPVISDADYDRLRRRYEDIEARFPALKNDDSLSRK
VGAAPSAKFAKVVHQVPMLSLQNAFSDEEVEEFVARVRRFLNLAESAELAFTAEPKIDGLSCSLRYEGGVLVRAATRGDG
TQGEDVTANVRTIAEIPHRLTGAGVPDIIDIRGEVYMGKADFLALNARQEEAGKPVFANPRNAAAGSLRQLDPEITRSRP
LRFFAYAWGQAEPGPPGASQHEVVDAFRRFGLPTNPLTIRVTDAAGLLAHYRKIGAERASLGYDIDGVVYKVDSLALQER
LGFVSRSPRWAIAHKFAAEQATTVIEAIEIQVGRTGALTPVAKLTPVTVGGVVVSNATLHNEDYIRAIGGNGEEIREGID
IRVGDTVVVQRAGDVIPQVVSVVLDKRPAGATAYQFPHHCPVCNSQAVREVDPKTGKQDAVRRCTGGLICPAQAVERLRH
FVSRNAFDIEGLGEKQAHAFYEWKLIAEPADIFRLQARNEQSLSKLENREGWGRTSAQNLFAAIEARRTIALDRFIFALG
IRHVGETNAKRLARHYGSVEALRDAAIGAEMPTEMEEASEETPPTRRRKPQGNEVWQEMTDIDGIGAVVAEAVVEFFREP
HNRQVVEELLKEVTPQPLEAVASTSPVSGKTVVFTGSLERMTRDEAKAMAERLGAKVAGSVSGKTDLLVAGPGAGSKLEK
ARSLGVKTITEDEWFDLVG

Sequences:

>Translated_739_residues
MTANRPALPTRDKAVSDLSATEASDEHAALSQEIASHDARYYQEDAPVISDADYDRLRRRYEDIEARFPALKNDDSLSRK
VGAAPSAKFAKVVHQVPMLSLQNAFSDEEVEEFVARVRRFLNLAESAELAFTAEPKIDGLSCSLRYEGGVLVRAATRGDG
TQGEDVTANVRTIAEIPHRLTGAGVPDIIDIRGEVYMGKADFLALNARQEEAGKPVFANPRNAAAGSLRQLDPEITRSRP
LRFFAYAWGQAEPGPPGASQHEVVDAFRRFGLPTNPLTIRVTDAAGLLAHYRKIGAERASLGYDIDGVVYKVDSLALQER
LGFVSRSPRWAIAHKFAAEQATTVIEAIEIQVGRTGALTPVAKLTPVTVGGVVVSNATLHNEDYIRAIGGNGEEIREGID
IRVGDTVVVQRAGDVIPQVVSVVLDKRPAGATAYQFPHHCPVCNSQAVREVDPKTGKQDAVRRCTGGLICPAQAVERLRH
FVSRNAFDIEGLGEKQAHAFYEWKLIAEPADIFRLQARNEQSLSKLENREGWGRTSAQNLFAAIEARRTIALDRFIFALG
IRHVGETNAKRLARHYGSVEALRDAAIGAEMPTEMEEASEETPPTRRRKPQGNEVWQEMTDIDGIGAVVAEAVVEFFREP
HNRQVVEELLKEVTPQPLEAVASTSPVSGKTVVFTGSLERMTRDEAKAMAERLGAKVAGSVSGKTDLLVAGPGAGSKLEK
ARSLGVKTITEDEWFDLVG
>Mature_738_residues
TANRPALPTRDKAVSDLSATEASDEHAALSQEIASHDARYYQEDAPVISDADYDRLRRRYEDIEARFPALKNDDSLSRKV
GAAPSAKFAKVVHQVPMLSLQNAFSDEEVEEFVARVRRFLNLAESAELAFTAEPKIDGLSCSLRYEGGVLVRAATRGDGT
QGEDVTANVRTIAEIPHRLTGAGVPDIIDIRGEVYMGKADFLALNARQEEAGKPVFANPRNAAAGSLRQLDPEITRSRPL
RFFAYAWGQAEPGPPGASQHEVVDAFRRFGLPTNPLTIRVTDAAGLLAHYRKIGAERASLGYDIDGVVYKVDSLALQERL
GFVSRSPRWAIAHKFAAEQATTVIEAIEIQVGRTGALTPVAKLTPVTVGGVVVSNATLHNEDYIRAIGGNGEEIREGIDI
RVGDTVVVQRAGDVIPQVVSVVLDKRPAGATAYQFPHHCPVCNSQAVREVDPKTGKQDAVRRCTGGLICPAQAVERLRHF
VSRNAFDIEGLGEKQAHAFYEWKLIAEPADIFRLQARNEQSLSKLENREGWGRTSAQNLFAAIEARRTIALDRFIFALGI
RHVGETNAKRLARHYGSVEALRDAAIGAEMPTEMEEASEETPPTRRRKPQGNEVWQEMTDIDGIGAVVAEAVVEFFREPH
NRQVVEELLKEVTPQPLEAVASTSPVSGKTVVFTGSLERMTRDEAKAMAERLGAKVAGSVSGKTDLLVAGPGAGSKLEKA
RSLGVKTITEDEWFDLVG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=714, Percent_Identity=46.6386554621849, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI87082305, Length=556, Percent_Identity=23.7410071942446, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_AZOC5 (A8IGY0)

Other databases:

- EMBL:   AP009384
- RefSeq:   YP_001523065.1
- ProteinModelPortal:   A8IGY0
- GeneID:   5691128
- GenomeReviews:   AP009384_GR
- KEGG:   azc:AZC_0149
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK979523
- BioCyc:   ACAU438753:AZC_0149-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 80557; Mature: 80425

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 136-136 BINDING 134-134 BINDING 157-157 BINDING 194-194 BINDING 311-311 BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANRPALPTRDKAVSDLSATEASDEHAALSQEIASHDARYYQEDAPVISDADYDRLRRR
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
YEDIEARFPALKNDDSLSRKVGAAPSAKFAKVVHQVPMLSLQNAFSDEEVEEFVARVRRF
HHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LNLAESAELAFTAEPKIDGLSCSLRYEGGVLVRAATRGDGTQGEDVTANVRTIAEIPHRL
HHHHHCCCEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
TGAGVPDIIDIRGEVYMGKADFLALNARQEEAGKPVFANPRNAAAGSLRQLDPEITRSRP
CCCCCCCEEECCCCEEECCCCEEEEECCHHHCCCCCCCCCCCCHHCHHHHCCCHHHHCCC
LRFFAYAWGQAEPGPPGASQHEVVDAFRRFGLPTNPLTIRVTDAAGLLAHYRKIGAERAS
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCHHHH
LGYDIDGVVYKVDSLALQERLGFVSRSPRWAIAHKFAAEQATTVIEAIEIQVGRTGALTP
CCCCCCHHEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
VAKLTPVTVGGVVVSNATLHNEDYIRAIGGNGEEIREGIDIRVGDTVVVQRAGDVIPQVV
HHHHCCEEECCEEEECCEECCCCCEEEECCCHHHHHCCCCEEECCEEEEEHHHHHHHHHH
SVVLDKRPAGATAYQFPHHCPVCNSQAVREVDPKTGKQDAVRRCTGGLICPAQAVERLRH
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
FVSRNAFDIEGLGEKQAHAFYEWKLIAEPADIFRLQARNEQSLSKLENREGWGRTSAQNL
HHCCCCCCCCCCCCHHHHHEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHH
FAAIEARRTIALDRFIFALGIRHVGETNAKRLARHYGSVEALRDAAIGAEMPTEMEEASE
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHC
ETPPTRRRKPQGNEVWQEMTDIDGIGAVVAEAVVEFFREPHNRQVVEELLKEVTPQPLEA
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
VASTSPVSGKTVVFTGSLERMTRDEAKAMAERLGAKVAGSVSGKTDLLVAGPGAGSKLEK
HHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHH
ARSLGVKTITEDEWFDLVG
HHHCCCCEECCCCHHHCCC
>Mature Secondary Structure 
TANRPALPTRDKAVSDLSATEASDEHAALSQEIASHDARYYQEDAPVISDADYDRLRRR
CCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
YEDIEARFPALKNDDSLSRKVGAAPSAKFAKVVHQVPMLSLQNAFSDEEVEEFVARVRRF
HHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LNLAESAELAFTAEPKIDGLSCSLRYEGGVLVRAATRGDGTQGEDVTANVRTIAEIPHRL
HHHHHCCCEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
TGAGVPDIIDIRGEVYMGKADFLALNARQEEAGKPVFANPRNAAAGSLRQLDPEITRSRP
CCCCCCCEEECCCCEEECCCCEEEEECCHHHCCCCCCCCCCCCHHCHHHHCCCHHHHCCC
LRFFAYAWGQAEPGPPGASQHEVVDAFRRFGLPTNPLTIRVTDAAGLLAHYRKIGAERAS
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHCCHHHH
LGYDIDGVVYKVDSLALQERLGFVSRSPRWAIAHKFAAEQATTVIEAIEIQVGRTGALTP
CCCCCCHHEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
VAKLTPVTVGGVVVSNATLHNEDYIRAIGGNGEEIREGIDIRVGDTVVVQRAGDVIPQVV
HHHHCCEEECCEEEECCEECCCCCEEEECCCHHHHHCCCCEEECCEEEEEHHHHHHHHHH
SVVLDKRPAGATAYQFPHHCPVCNSQAVREVDPKTGKQDAVRRCTGGLICPAQAVERLRH
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
FVSRNAFDIEGLGEKQAHAFYEWKLIAEPADIFRLQARNEQSLSKLENREGWGRTSAQNL
HHCCCCCCCCCCCCHHHHHEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHH
FAAIEARRTIALDRFIFALGIRHVGETNAKRLARHYGSVEALRDAAIGAEMPTEMEEASE
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHC
ETPPTRRRKPQGNEVWQEMTDIDGIGAVVAEAVVEFFREPHNRQVVEELLKEVTPQPLEA
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
VASTSPVSGKTVVFTGSLERMTRDEAKAMAERLGAKVAGSVSGKTDLLVAGPGAGSKLEK
HHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCHHHHH
ARSLGVKTITEDEWFDLVG
HHHCCCCEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA